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HvMPK4 phosphorylates HvWRKY1 to enhance its suppression of barley immunity to powdery mildew fungus

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Journal of Genetics and Genomics 2024年第3期51卷 313-325页

作者： Pengya Xue Ling Zhang Renchun Fan Yanan Li Xinyun Han Ting Qi Lifang Zhao deshui yu Qian-Hua Shen State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyInnovation Academy for Seed DesignChinese Academy of SciencesBeijing 100101China CAS Center for Excellence in Biotic Interactions College of Advanced Agricultural SciencesUniversity of Chinese Academy of SciencesBeijing 100049China Department of Nutritional Sciences&Toxicology University of CaliforniaBerkeleyCA 94720USA Lushan Botanical Garden Chinese Academy of ScienceJiujiangJiangxi 332900China

Mitogen-activated protein kinase(MAPK)cascades play important roles in disease resistance in model plant ***,the functions of MAPK signaling pathways in crop disease resistance are largely *** we report the function of HvMKK1-HvMPK4-HvWRKY1 module in barley immune ***4 is identified to play a negative role in barley immune response against Bgh,as virus-induced gene silencing of HvMPK4 results in enhanced disease resistance whilst stably overexpressing HvMPK4 leads to super-susceptibility to Bgh ***,the barley MAPK kinase HvMKK1 is found to specifically interact with HvMPK4,and the activated HvMKK1^(DD) variant specifically phosphorylates HvMPK4 in ***,the transcription factor HvWRKY1 is identified to be a downstream target of HvMPK4 and phosphorylated by HvMPK4 in vitro in the presence of HvMKK1^(DD).Phosphorylation assay coupled with mutagenesis analyses identifies S122,T284,and S347 in HvWRKY1 as the major residues phosphorylated by ***1 is phosphorylated in barley at the early stages of Bgh infection,which enhances its suppression on barley immunity likely due to enhanced DNA-binding and transcriptional repression *** data suggest that the HvMKK1-HvMPK4 kinase pair acts upstream of HvWRKY1 to negatively regulate barley immunity against powdery mildew.

关键词： Barley MPK4 WRKY1 Phosphorylation Disease resistance Powdery mildew

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流向图在行星齿轮箱故障诊断中的应用

中国机械工程 2018年第10期29卷 1208-1213页

作者：于军于德水哈尔滨理工大学自动化学院哈尔滨150080 哈尔滨工业大学机电学院哈尔滨150001

针对行星齿轮箱诊断过程复杂和诊断结果准确率低的问题,提出了一种基于流向图的行星齿轮箱故障诊断方法。该方法通过流向图构建算法直观地表示行星齿轮箱的故障诊断知识,利用流向图约简算法删除不必要的征兆属性节点,以简洁的形式表示... 详细信息

针对行星齿轮箱诊断过程复杂和诊断结果准确率低的问题,提出了一种基于流向图的行星齿轮箱故障诊断方法。该方法通过流向图构建算法直观地表示行星齿轮箱的故障诊断知识,利用流向图约简算法删除不必要的征兆属性节点,以简洁的形式表示属性之间的因果关系,利用流向图分类决策算法确定待诊实例的故障类型。仿真和实验结果证明了该方法的准确性和直观性,为行星齿轮箱的故障诊断提供了一种新颖的解决思路。

关键词：流向图行星齿轮箱故障诊断约简分类决策

Targeted Mutagenesis of NAC Transcription Factor Gene,OsN AC041,Leading to Salt Sensitivity in Rice

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Rice science 2019年第2期26卷 98-108,I0007-I0015页

作者： WANG Bo ZHONG Zhaohui ZHANG Huanhuan WANG Xia LIU Binglin YANG Lijia HAN Xiangyan yu deshui ZHENG Xuelian WANG Chunguo SONG Wenqin CHEN Chengbin ZHANG Yong College of Life Sciences Nankai University Department of Biotechnology School of Life Sciences and Technology Center for Informational Biology University of Electronic Science and Technology of China

Salinity is a major abiotic stress factor that seriously affects plant growth. Many genes are involved in the response to salt stress with various metabolism pathways. A number of plant transcription factor family genes have been found to be involved in the salt stress response, and NAM, ATAF and CUC(NAC) transcription factors are thought to act as active regulators during abiotic stress, especially salt stress. In this study, we detected a rice NAC transcription factor coding gene, OsNAC041, and confirmed that it influenced the germination of seeds under salt stress and salt tolerance of plants. OsNAC041 was primarily expressed in the leaves and located in the nucleus. Furthermore, the CRISPR/Cas9 method was used to obtain a targeted osnac041 mutant, of which the plant height was higher than that of the wild-type, showing increased salt sensitivity. Moreover, RNA-seq analysis revealed a number of differentially expressed genes(DEGs) involved in several important signaling pathways in the osnac041 mutant. Subsequently, Kyoto Encyclopedia of Genes and Genomes annotation also revealed differential expression of DEGs associated with mitogen-activated protein kinase signaling, peroxisome, eukaryotictype ABC transporters, photosynthesis and plant hormones, which are involved in stress-related signaling pathways. Overall, our study suggested that OsNAC041 was involved in the salt stress response in rice. These findings not only provide empirical evidence of OsNAC041 function, but also provide new insight into its potential application in rice resistance breeding.

关键词： rice NAC transcription factor CRISPR Cas9 RNA-seq salinity

HvWRKY2 acts as an immunity suppressor and targets HvCEBiP to regulate powdery mildew resistance in barley

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The Crop Journal 2023年第1期11卷 99-107页

作者： deshui yu Renchun Fan Ling Zhang Pengya Xue Libing Liao Meizhen Hu Yanjun Cheng Jine Li Ting Qi Shaojuan Jing Qiuyun Wang Arvind Bhatt Qian-Hua Shen Lushan Botanical Garden Chinese Academy of ScienceJiujiang 332900JiangxiChina State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyChinese Academy of SciencesInnovation Academy for Seed DesignBeijing 100101China CAS Center for Excellence in Biotic Interactions University of Chinese Academy of SciencesBeijing 100049China

Plants use a sophisticated immune system to perceive pathogen infection and activate immune responses in a tightly controlled *** barley,Hv WRKY2 acts as a repressor in barley disease resistance to the powdery mildew fungus,Blumeria graminis ***(Bgh).However,the molecular features of Hv WRKY2 in its DNA-binding and repressor functions,as well as its target genes,are *** show that the W-box binding of Hv WRKY2 requires an intact WRKY domain and an upstream sequence of~75 amino acids,and the Hv WRKY2 W-box binding activity is linked to its repressor function in disease *** immunoprecipitation(ChIP)-seq analysis identified HvCEBiP,a putative chitin receptor gene,as a target gene of Hv WRKY2 in overexpressing transgenic barley ***-qPCR and Electrophoretic Mobility Shift Assay(EMSA)verified the direct binding of Hv WRKY2 to a W-boxcontaining sequence in the HvCEBiP *** CEBiP positively regulates resistance against Bgh in *** findings suggest that Hv WRKY2 represses barley basal immunity by directly targeting pathogen-associated molecular pattern(PAMP)recognition receptor genes,suggesting that Hv CEBiP and likely chitin signaling function in barley PAMP-triggered immune responses to Bgh infection.

关键词： HvWRKY2 Basal immunity Chromatin immunoprecipitation(ChIP) HvCEBiP

抑制miR-429促进ZO-1、Occludin和Claudin-5蛋白表达改善血脊髓屏障通透性的体外实验研究

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中国修复重建外科杂志 2020年第9期34卷 1163-1169页

作者：孙瑞于德水锦州医科大学附属第一医院骨创伤科辽宁锦州121000 辽宁省健康产业集团阜新矿业集团总医院骨科辽宁阜新123000

目的探讨抑制miR-429改善体外血脊髓屏障(blood spinal cord barrier,BSCB)通透性的可行性及机制,为改善脊髓微环境提供新的基因治疗靶点。方法首先,取永生化人脑微血管内皮细胞系(hCMEC/D3),应用miR-429拮抗剂antagomiR-429及其阴性对... 详细信息

目的探讨抑制miR-429改善体外血脊髓屏障(blood spinal cord barrier,BSCB)通透性的可行性及机制,为改善脊髓微环境提供新的基因治疗靶点。方法首先,取永生化人脑微血管内皮细胞系(hCMEC/D3),应用miR-429拮抗剂antagomiR-429及其阴性对照antagomiR-429-NC进行转染,通过荧光显微镜观察以及实时荧光定量PCR检测miR-429表达,验证antagomiR-429转染效率。然后观察miR-429对体外BSCB通透性的影响。实验分为4组,其中空白对照组(A组)为正常hCMEC/D3细胞、Ha-sc细胞构建BSCB模型,低氧诱导组(B组)、低氧诱导+antagomiR-429-NC组(C组)、低氧诱导+antagomiR-429组(D组)分别采用正常、antagomiR-429-NC转染以及antagomiR-429转染的hCMEC/D3细胞,与Ha-sc细胞构建BSCB模型并低氧处理12 h。通过辣根过氧化物酶(horseradish peroxidase,HRP)通量测定体外BSCB通透性,实时荧光定量PCR、Western blot和免疫荧光染色观测内皮细胞中紧密连接蛋白ZO-1、Occludin、Claudin-5的表达水平。结果荧光显微镜下观察antagomiR-429及antagomiR-429-NC成功转染至hCMEC/D3细胞,转染效率约为90%;实时荧光定量PCR检测antagomiR-429组miR-429相对表达量为0.109±0.013,明显低于antagomiR-429-NC组的0.956±0.004(P0.05),D组尚未达A组水平(P<0.05)。免疫荧光染色观察示D组ZO-1、Occludin和Claudin-5蛋白在细胞膜边界免疫荧光较B、C组增强,但尚未达A组强度。结论通过抑制miR-429表达可促进微血管内皮细胞中ZO-1、Occludin和Claudin-5蛋白表达,进而改善因低氧导致的BSCB通透性增高。

关键词：血脊髓屏障 miR-429 脊髓损伤紧密连接蛋白脊髓微环境

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水包油乳状液的反相破乳剂选择与制备

油气田地面工程 2008年第7期27卷 31-33页

作者：于德水大庆职业学院

大庆油田近年来开展的三元(碱、表面活性剂、聚合物)复合驱矿场实验取得了比水驱和聚合物驱分别提高采收率17%～20%和10%的良好效果,但采出液中残留的碱、表面活性剂和聚合物导致油水乳化程度加大,给采出液的油水分离带来了困难。为此,... 详细信息

大庆油田近年来开展的三元(碱、表面活性剂、聚合物)复合驱矿场实验取得了比水驱和聚合物驱分别提高采收率17%～20%和10%的良好效果,但采出液中残留的碱、表面活性剂和聚合物导致油水乳化程度加大,给采出液的油水分离带来了困难。为此,根据大庆油田三元复合驱采出液的油水分离特性,以模拟采出液为介质,采用均匀设计方法研制了一种针对大庆油田三元复合驱采出液的破乳剂,并测试了该破乳剂对模拟三元复合驱采出液和实际三元复合驱采出液的破乳效果。

关键词：反相破乳剂改性制备测试

基于最先进的光钟直接演示自旋压缩引起的计量增强

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Science Bulletin 2024年第10期69卷 1359-1361页

作者：陈景标于得水 State Key Laboratory of Advanced Optical Communication System and NetworkSchool of ElectronicsPeking UniversityBeijing 100871China National Time Service Center Chinese Academy of SciencesXi’an 710600China Key Laboratory of Time Reference and Applications Chinese Academy of SciencesXi’an 710600China

Benefiting from the recent progress in precise engineering of quantum states and sub-10 mHz linewidth lasers,the stability and accuracy of optical atomic clocks have reached and even exceeded the 1018 level,surpassing their microwave counterparts by over two orders of magnitude[1,2].These advances lead to an anticipated redefinition of the international system of units(SI)second in terms of an optical reference transition as early as 2026.

关键词： optical 光钟自旋压缩

维普期刊数据库评论

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萨北油田曲流型河道砂体建筑结构研究

断块油气田 2011年第1期18卷 30-33页

作者：于德水大庆油田有限责任公司勘探开发研究院黑龙江大庆163712

面对高含水后期影响储层剩余油分布的砂体内部建筑结构等深层次的储层非均质性问题,特别对于曲流型河道砂体而言,其纵向上的非均质性及内部单砂体建筑结构是影响剩余油分布的主要因素。以现代沉积和野外露头理论模式为指导,应用Miall的... 详细信息

面对高含水后期影响储层剩余油分布的砂体内部建筑结构等深层次的储层非均质性问题,特别对于曲流型河道砂体而言,其纵向上的非均质性及内部单砂体建筑结构是影响剩余油分布的主要因素。以现代沉积和野外露头理论模式为指导,应用Miall的层次界面和结构要素分析法,采用"层次分析、模式预测"研究思路,结合油田密井网资料,对萨北油田北二西区萨Ⅱ1+2b沉积单元曲流型河道砂体建筑结构进行分级精细解剖。应用"废弃河道定边,砂体厚度定位"方法综合识别点坝砂体分布;详细分析砂体内部侧积体和侧积夹层空间分布规律,并求得点坝内部定量判断侧积层倾角的经验公式;在此基础上,建立基于井点数据的砂体内部三级界面-侧积夹层三维地质模型,为高含水后期综合调整,剩余油挖潜提供地质依据。

关键词：建筑结构河道砂体点坝侧积夹层

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提升烟草行业软实力的思考和建议

中国烟草学报 2020年第5期26卷 107-113页

作者：于得水李保江国家烟草专卖局烟草经济研究所(政策研究室) 100045

基于软实力理论研究成果、国家软实力建设要求和烟草行业软实力的实际现状,本文探讨了烟草行业软实力的内涵和构成要素,即文化感召力、战略领导力、学习创新力、社会责任力、舆论引导力和风险抵御力。遵循软实力建设规律,提出了把观念... 详细信息

基于软实力理论研究成果、国家软实力建设要求和烟草行业软实力的实际现状,本文探讨了烟草行业软实力的内涵和构成要素,即文化感召力、战略领导力、学习创新力、社会责任力、舆论引导力和风险抵御力。遵循软实力建设规律,提出了把观念变革作为先决条件,把顶层规划和机制建设作为前提支撑,把处理好“三个关系”作为重要原则,把加强智库建设作为重点工程,把提升烟草行业形象作为落脚点的基本要求,并从烟草行业软实力构成的六个方面分别给出了相应提升建议。

关键词：烟草行业软实力构成要素提升建议