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Evaluation of the sperm DNA fragmentation index in infertile Japanese men by in-house flow cytometric analysis

Asian Journal of Andrology 2022年第1期24卷 40-44页

作者： Akiyoshi Osaka Hiroshi Okada Sae Onozuka Takashi Tanaka Toshiyuki Iwahata Yukihito Shimomura yoshitomo kobori Kazutaka Saito Kouhei Sugimoto Department of Urology Dokkyo Medical University Saitama Medical Center2-1-50 MinamikoshigayaKoshigayaSaitama 343-8555Japan International Center for Reproductive Medicine Dokkyo Medical University Saitama Medical Center2-1-50 MinamikoshigayaKoshigayaSaitama 343-8555Japan

Semen analysis has long been used to evaluate male ***,several sperm function tests have been *** those,the sperm DNA fragmentation index(DFI),which describes the status of the sperm DNA,is thought to be a suitable parameter for evaluating male ***,there have been no large-scale studies on the sperm DFI of Japanese ***,we investigated the feasibility of using an in-house flow cytometry-based sperm DFI analysis based on the sperm DNA fragmentation test of sperm chromatin structure assay(SCSA)to assess male fertility in *** study enrolled 743 infertile and 20 fertile Japanese *** evaluate reproducibility,inter-and intraobserver precision was analyzed.A receiver operating characteristic curve analysis was used to set a cutoff value for the sperm DFI to identify men who could father children by timed intercourse or intrauterine *** variability of the sperm DFI among fertile volunteers was *** relationship between semen parameters and the sperm DFI was assessed by Spearman’s rho test.A precision analysis revealed good reproducibility of the sperm *** cutoff value of sperm DNA fragmentation in infertile men was 24.0%.Semen volume had no relationship with the sperm *** concentration,sperm motility,total motile sperm count,and percentage of normal-shaped sperm were significantly and negatively correlated with the sperm *** median sperm DFI was smaller in fertile volunteers(7.7%)than that in infertile men(19.4%).Sperm DNA fragmentation analysis can be used to assess sperm functions that cannot be evaluated by ordinary semen analysis.

关键词： DNA fragmentation flow cytometry male infertility semen analysis spermatozoa

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Y chromosome microdeletion screening using a new molecular diagnostic method in 1030 Japanese males with infertility

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Asian Journal of Andrology 2020年第4期22卷 368-371页

作者： Masashi Iijima Kazuyoshi Shigehara Hideki Igarashi Koichi Kyono Yasuo Suzuki Yuji Tsuji yoshitomo kobori Hideyuki Kobayashi Atsushi Mizokami Department of Integrative Cancer Therapy and Urology Kanazawa University Graduate School of Medical ScienceKanazawa 920-8641Japan Kyono ART Clinic Sendai 980-0014Japan Kyono ART Clinic TakanawaTokyo 108-0074Japan Suzuki Lady's Hospital Kanazawa 921-8033Japan Ebisu Tsuji Clinic Tokyo 150-0021Japan Tenjin Tsuji Clinic Fukuoka 810-0001Japan Dokkyo Medical University Koshigaya Hospital Saitama 343-8555Japan Department of Urology Faculty of MedicineToho UniversityTokyo 143-8541Japan

The azoospermia factor(AZF)region is important for spermatogenesis,and deletions within these regions are a common cause of oligozoospermia and *** several studies have reported this cause,the present research,to the best of our knowledge,is the first large-scale study assessing this factor in *** this study,1030 male patients with infertility who were examined for Y chromosome microdeletion using the polymerase chain reaction-reverse sequence-specific oligonucleotide(PCR-rSSO)method,a newly developed method for Y chromosome microdeletion screening,were *** study enrolled 250 patients with severe oligospermia and 717 patients with *** the 1030 patients,4,4,10,and 52 had AZFa,AZFb,AZFb+c,and AZFc deletions,*** sperm recovery rate(SRR)of microdissection testicular sperm extraction in patients with AZFc deletions was significantly higher than that in those without AZF deletions(60.0%vs 28.7%,P=0.04).In patients with gr/gr deletion,SRR was 18.7%,which was lower than that in those without gr/gr deletion,but was not statistically *** conclusion,our study showed that the frequency of Y chromosome microdeletion in male patients in Japan was similar to that reported in patients from other countries,and SRR was higher in patients with AZFc deletion.

关键词： azoospermia factor Japanese infertile men sperm recovery rate Y chromosome microdeletion

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Genomics, Proteomics & Bioinformatics 2016年第2期14卷 103-112页

作者： Yusei kobori Satoshi Mizuta Graduate School of Science and Technology Hirosaki University

Graphical representation of DNA sequences is one of the most popular techniques for alignment-free sequence comparison. Here, we propose a new method for the feature extraction of DNA sequences represented by binary images, by estimating the similarity between DNA sequences using the frequency histograms of local bitmap patterns of images. Our method shows linear time complexity for the length of DNA sequences, which is practical even when long sequences, such as whole genome sequences, are compared. We tested five distance measures for the estimation of sequence similarities, and found that the histogram intersection and Manhattan distance are the most appropriate ones for phylogenetic analyses.

关键词： Genome sequence Mitochondria Bitmap image Occurrence frequency Distance measure

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Manipulation of Charge-Transfer States by Molecular Design:Perspective from“Dynamic Exciton”

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Accounts of Materials Research 2021年第7期2卷 501-514页

作者： Hiroshi Imahori Yasuhiro kobori Hironori Kaji Department of Molecular Engineering Graduate School of EngineeringKyoto UniversityNishikyokuKyoto 615-8510Japan Institute for Integrated Cell-Material Sciences(WPI-iCeMS) Kyoto UniversitySakyo-kuKyoto 606-8501Japan Molecular Photoscience Research Center and Department of Chemistry Graduate School of ScienceKobe UniversityNada-kuKobe 657-8501Japan Institute for Chemical Research Kyoto UniversityUjiKyoto 611-0011Japan

CONSPECTUS:“Dynamic exciton”,an umbrella term concept in photochemistry,plays an important role in nature,science,and technology,especially in photoinduced and electrically induced electron transfer(ET)between a donor(D)and an acceptor(A).Typically,an exciton in molecular D−A systems is considered a locally excited(LE)state of a donor(D*)or an acceptor(A*)molecule,but let us extend the terminology of“exciton”to an integrated class of LE,charge-transfer(CT),and charge-separated(CS)*** degree of CT(0−100%),spin multiplicity,and D−A interaction(i.e.,electronic coupling)are pivotal factors in the“exciton”.Another important aspect of the“exciton”is strongly related to the“dynamic”aspect of the“exciton”by movement of atomic nuclei(i.e.,vibration,rotation,and fluctuation)and their collective motions controlling behaviors of electrons and spins by the passage of *** concept of“dynamic exciton”should cover a wide variety of the photochemical phenomena that are all essential for the energy conversion devices and processes including various kinds of living *** these,a huge amount of the nuclear motional modes may cooperatively be entangled to electronic ***,the idea behind“dynamic exciton”includes usage of the cooperation between nuclear motions and the spin−orbital,as electron−phonon coupling for innovative designs of the energy conversion materials and *** this,it is particularly important to examine how this wide variety of the motions plays roles on electronic couplings,intermediate geometries and mobilities,exciton energies,CT characters,and so *** draw researchers’attention to this aspect of the vibronic effect,like the entropy role by collective movement of side chains of the conjugated polymer to modulate the electronic coupling time-dependently at the charge delocalization and dissociation,which is reinforced three-dimensionally at the D/A domain interface of organic photovoltaics(OPVs).In addition,to realize the ultimate high

关键词： excited vibrational collective

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Raman lasing and soliton mode-locking in lithium niobate microresonators

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Light(Science & Applications) 2020年第1期9卷 1925-1931页

作者： Mengjie Yu yoshitomo Okawachi Rebecca Cheng Cheng Wang Mian Zhang Alexander L.Gaeta Marko Loncar Department John A.Paulson School of Engineering and Applied Sciences Harvard UniversityCambridgeMA 02138USA Department of Applied Physics and Applied Mathematics Columbia UniversityNew YorkNY 10027USA Department of Electronic Engineering&State Key Laboratory of Terahertz and Millimeter Waves City University of Hong KongKowloonHong KongChina HyperLight Corporation 501 Massachusetts AvenueCambridgeMA 02139USA

The recent advancement in lithium-niobite-on-insulator(LNOI)technology is opening up new opportunities in optoelectronics,as devices with better performance,lower power consumption and a smaller footprint can be realised due to the high optical confinement in the *** LNOI platform offers both largeχ(2)andχ(3)nonlinearities along with the power of dispersion engineering,enabling brand new nonlinear photonic devices and applications for the next generation of integrated photonic ***,Raman scattering and its interaction with other nonlinear processes have not been extensively studied in dispersion-engineered LNOI *** this work,we characterise the Raman radiation spectra in a monolithic lithium niobate(LN)microresonator via selective excitation of Raman-active phonon *** dominant mode for the Raman oscillation is observed in the backward direction for a continuous-wave pump threshold power of 20mW with a high differential quantum efficiency of 46%.We explore the effects of Raman scattering on Kerr optical frequency comb *** achieve mode-locked states in an X-cut LNOI chip through sufficient suppression of the Raman effect via cavity geometry *** analysis of the Raman effect provides guidance for the development of future chip-based photonic devices on the LNOI platform.

关键词： scattering pump lithium

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Non-improvement of atrophic gastritis in cases of gastric cancer after successful Helicobacter pylori eradication therapy

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World Journal of Clinical Cases 2024年第14期12卷 2342-2349页

作者： Yuto Suzuki Yasumi Katayama Yo Fujimoto Ikuhiro kobori Masaya Tamano Department of Gastroenterology Dokkyo medical University Saitama Medical CenterKoshigayaSaitama 343-8555Japan Endoscopy Center Dokkyo Medical University Saitama Medical CenterKoshigayaSaitama 343-8555Japan

BACKGROUND Helicobacter pylori(***)infection is closely related to the development of gastric cancer(GC).However,GC can develop even after *** ***,it would be extremely useful if GC could be predicted after *** Kyoto classification score for gastritis(GA)is closely related to cancer ***,how the score for GC changes after eradication before onset is not well *** To investigate the characteristics of the progression of Kyoto classification scores for GC after *** *** Eradication of *** was confirmed in all patients using either the urea breath test or the stool antigen *** Kyoto classification score of GC patients was evaluated by endoscopy at the time of event onset and three years *** ad-dition,the modified atrophy score was evaluated and compared between the GC group and the control GA *** In total,30 cases of early GC and 30 cases of chronic GA were *** pathology of the cancer cases was differentiated adenocarcinoma,except for one case of undifferentiated *** total score of the Kyoto classifi-cation was significantly higher in the GC group both at the time of cancer onset and three years earlier(4.97 vs 3.73,P=0.0034;4.2 vs 3.1,P=0.0035,respectively).The modified atrophy score was significantly higher in the GC group both at the time of cancer onset and three years earlier and was significantly improved only in the GA group(5.3 vs 5.3,P=0.5;3.73 vs 3.1,P=0.0475,respectively).CONCLUSION The course of the modified atrophy score is useful for predicting the onset of GC after *** with severe atrophy after *** eradication require careful monitoring.

关键词： Helicobacter pylori Kyoto classification Gastritis Eradication therapy Gastric cancer

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Butyrate stimulates IL-32α expression in human intestinal epithelial cell lines

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World Journal of Gastroenterology 2010年第19期16卷 2355-2361页

作者： Ayako kobori Shigeki Bamba Hirotsugu Imaeda Hiromitsu Ban Tomoyuki Tsujikawa Yasuharu Saito Yoshihide Fujiyama Akira Andoh Division of Gastroenterology Department of Internal Medicine Shiga University of Medical Science Seta Tukinowa Otsu 520-2192 Japan Division of Mucosal Immunology Graduate School of Medicine Shiga University of Medical Science Seta Tukinowa Otsu 520-2192 Japan

AIM: To investigate the effects of butyrate on interleukin (IL)-32α expression in epithelial cell lines. METHODS: The human intestinal epithelial cell lines HT-29, SW480, and T84 were used. Intracellular IL- 32α was determined by Western blotting analyses. IL- 32α mRNA expression was analyzed by real-time poly-merase chain reaction. RESULTS: Acetate and propionate had no effects on IL-32α mRNA expression. Butyrate significantly enhanced IL-32α expression in all cell lines. Butyrate also up-regulated IL-1β-induced IL-32α mRNA expression. Butyrate did not modulate the activation of phosphatidylinositol 3-kinase (PI3K), a mediator of IL- 32α expression. Like butyrate, trichostatin A, a histone deacetylase inhibitor, also enhanced IL-1β-induced IL- 32α mRNA ***: Butyrate stimulated IL-32α expression in epithelial cell lines. An epigenetic mechanism, such as histone hyperacetylation, might be involved in the action of butyrate on IL-32α expression.

关键词： Cytokine Histone acetylation Inflammatory bowel disease

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Progress of ulcerative colitis patients during the COVID-19 pandemic

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World Journal of Clinical Cases 2023年第23期11卷 5462-5467页

作者： Toshikuni Suda Morio Takahashi Yasumi Katayama Koichi Soga Ikuhiro kobori Yumi Kusano Masaya Tamano Division of Gastroenterology Dokkyo Medical University Saitama Medical CenterSaitama 343-8555Japan Division of Gastroenterology Morio ClinicSaitama 343-0808Japan

BACKGROUND We have previously demonstrated that the first wave of the coronavirus disease 2019(COVID-19)pandemic caused exacerbations in ulcerative colitis(UC)patients,probably through psychological and physical ***,successive waves of the COVID-19 pandemic continuously followed the *** effects of this chronic stress on the disease condition in UC patients are of *** To clarify the effect of chronic stress from COVID-19 on disease condition in patients aggravated after the first *** Our previous study investigated 289 consecutive UC outpatients treated in one center during March and April 2020,the period of the first wave of the COVID-19 *** this study,an identical group of 289 UC patients was evaluated using UC-disease activity index(UC-DAI),endoscopic mucosal appearance score,and Matts pathological grade *** Of the 289 UC patients included in the study in 2020,10 patients dropped out as of 2021 and another 11 patients dropped out as of 2022,making three groups for 2020,2021 and *** significant differences in characteristics were found among the three ***-DAI scores had aggravated during the period of the first wave of the COVID-19 pandemic,but significantly recovered in 2021 and remained stable in *** grade scores significantly recovered in 2021 from those in 2020 and remained stable in *** Disease activity of UC patients recovered in 2021 and remained stable in 2022,aggravated by the stress of the first wave of COVID-19 in 2020 despite persistence of the pandemic.

关键词： Ulcerative colitis COVID-19 Exacerbation Stress Ulcerative colitis disease activity index Matts grade

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RANKL^(+) senescent cells under mechanical stress: a therapeutic target for orthodontic root resorption using senolytics

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International Journal of Oral Science 2023年第2期15卷 278-290页

作者： Yue Zhou Aki Nishiura Hidetoshi Morikuni Wenqi Deng Toru Tsujibayashi Yoshihiro Momota Yuki Azetsu Masamichi Takami yoshitomo Honda Naoyuki Matsumoto Department of Orthodontics Osaka Dental UniversityHirakataOsakaJapan Department of Physics Osaka Dental UniversityHirakataOsakaJapan Department of Anesthesiology Osaka Dental UniversityHirakataOsakaJapan Department of Pharmacology Showa University School of Dentistry1-5-8HatanodaiShinagawakuTokyoJapan Department of Oral Anatomy Osaka Dental University8-1 KuzuhahanazonochoHirakataOsakaJapan

In dentistry,orthodontic root resorption is a long-lasting issue with no effective treatment strategy,and its mechanisms,especially those related to senescent cells,remain largely ***,we used an orthodontic intrusion tooth movement model with an L-loop in rats to demonstrate that mechanical stress-induced senescent cells aggravate apical root resorption,which was prevented by administering senolytics(a dasatinib and quercetin cocktail).

关键词： stress therapeutic ortho

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Angiotensinogen Expression Is Enhanced in the Progression of Glomerular Disease

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International Journal of Clinical Medicine 2011年第4期2卷 378-387页

作者： Maki Urushihara Hiroyuki kobori Department of Physiology and Hypertension and Renal Center of Excellence Tulane University Health Sciences Center New Orleans USA.

Intrarenal renin-angiotensin system (RAS) activation plays a critical role in the development and progression of renal injury. In the kidney, all of the RAS components are present and intrarenal angiotensin II (Ang II) is formed by multiple independent mechanisms. Angiotensinogen (AGT) is the only known substrate for renin that is a rate-limiting enzyme of the RAS. Recently, enhanced intrarenal AGT levels have been shown to reflect the intrarenal RAS status in hypertension, chronic glomerular disease and diabetic nephropathy. In this review, we focus on AGT expression of the diseased glomeruli in the progression of glomerular disease. An anti-glomerular basement membrane nephritis rat model developed progressive proteinuria and glomerular crescent formation, accompanied by increased macrophage infiltration and glomerular expression of AGT and Ang II. The addition of Ang II type 1 receptor blocker to CC-chemokine recaptor 2 antagonist markedly attenuated the induction of macrophage infiltration, AGT and Ang II, and reduced glomerular crescent formation. Next, the levels of glomerular AGT expression and marker of reactive oxygen species in Zucker diabetic fatty (ZDF) obese rats were higher than those in ZDF lean rats. Hydrogen peroxide (H2O2) induced an increase in the AGT expression in primary rat mesangial cells. Furthermore, the H2O2-induced upregulation of AGT was inhibited by a mitogen-activated protein kinase kinase and a c-Jun N-terminal kinase inhibitor. These data suggest the potential contribution of enhanced AGT expression in glomeruli to the intrarenal RAS activation for the development of glomerular disease.

关键词： Renin-Angiotensin System Angiotensinogen Glomerulonephritis Glomerulosclerosis

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