Evaluation of the sperm DNA fragmentation index in infertile Japanese men by in-house flow cytometric analysis

作     者：Akiyoshi Osaka Hiroshi Okada Sae Onozuka Takashi Tanaka Toshiyuki Iwahata Yukihito Shimomura Yoshitomo Kobori Kazutaka Saito Kouhei Sugimoto Akiyoshi Osaka;Hiroshi Okada;Sae Onozuka;Takashi Tanaka;Toshiyuki Iwahata;Yukihito Shimomura;Yoshitomo Kobori;Kazutaka Saito;Kouhei Sugimoto

作者机构：Department of UrologyDokkyo Medical University Saitama Medical Center2-1-50 MinamikoshigayaKoshigayaSaitama 343-8555Japan International Center for Reproductive MedicineDokkyo Medical University Saitama Medical Center2-1-50 MinamikoshigayaKoshigayaSaitama 343-8555Japan 

出 版 物：《Asian Journal of Andrology》 (亚洲男性学杂志（英文版）)

年 卷 期：2022年第24卷第1期

页      面：40-44页

核心收录：

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

基　　金：The authors received funding from the Japan Agency for Medical Research and Development(AMED grant No.17930555)for this work 

主　　题：DNA fragmentation flow cytometry male infertility semen analysis spermatozoa 

摘      要：Semen analysis has long been used to evaluate male ***,several sperm function tests have been *** those,the sperm DNA fragmentation index(DFI),which describes the status of the sperm DNA,is thought to be a suitable parameter for evaluating male ***,there have been no large-scale studies on the sperm DFI of Japanese ***,we investigated the feasibility of using an in-house flow cytometry-based sperm DFI analysis based on the sperm DNA fragmentation test of sperm chromatin structure assay(SCSA)to assess male fertility in *** study enrolled 743 infertile and 20 fertile Japanese *** evaluate reproducibility,inter-and intraobserver precision was analyzed.A receiver operating characteristic curve analysis was used to set a cutoff value for the sperm DFI to identify men who could father children by timed intercourse or intrauterine *** variability of the sperm DFI among fertile volunteers was *** relationship between semen parameters and the sperm DFI was assessed by Spearman’s rho test.A precision analysis revealed good reproducibility of the sperm *** cutoff value of sperm DNA fragmentation in infertile men was 24.0%.Semen volume had no relationship with the sperm *** concentration,sperm motility,total motile sperm count,and percentage of normal-shaped sperm were significantly and negatively correlated with the sperm *** median sperm DFI was smaller in fertile volunteers(7.7%)than that in infertile men(19.4%).Sperm DNA fragmentation analysis can be used to assess sperm functions that cannot be evaluated by ordinary semen analysis.