检索结果-南通市图书馆

Chemical Research in Chinese Universities 2002年第2期18卷 206-210页

作者： LI Bo yoshifumi koide Kunio ESUMI Toyota Central R&D Labs. Inc.411YokomichiNagakuteAichi 4801192Japan Departemnt of Applied Chemistry and Institute of Colloid and Interface Science Science University of Tokyo

Calix resorcinarenes bearing four hydrophobic side chains ( Ar-Rn, Ar-Ph, and Ar-N) orient stably on water oil interface and show high solubilization capacities for organic compounds, such as long chain alcohols, benzene, toluene and dyes. The capacities are high even near the cmc of polyalkylated calix resorcinarenes, but the solubilities of organic compounds(solubilizates) decrease with the increase of the size of the solubilizates. Ar-R 6, with a chain length of six carbons, is the most effective among Ar-R n , Ar-Ph, and Ar-N; 11 fold mol of hexanol is dissolved in 2×10 -3 mol/L Ar-R 6. Moreover, Ar-R n bearing four alkyl side chains solubilizes the chain alcohols of the same chain length mostly. The solubilization capacities are presumably brought by inclusion in a large cavity of the polyalkylated calix resorcinarenes and by an efficient orientation of the solubilizates.

关键词： Calix resorcinarene Hydrophobic chain Polyalkylate Solubilizate

维普期刊数据库

Evolution of the “Neutral” : Diverse Forms of Wide-Compatibility Haplotypes at the Locus for Reproductive Isolation

在线全文

学校读者我要写书评

暂无评论

Molecular Plant 2020年第4期13卷 539-540页

作者： Yohei koide Research Faculty of Agriculture Hokkaido UniversityKita 9 Nishi 9SapporoHokkaido 060-8589Japan

One of the biggest questions in evolutionary biology is how biological species *** biological species are groups of interbreeding natural populations reproductively isolated from other such groups(Mayer,1942),reproductive isolation is the key process of the *** various isolating barriers,postzygotic barriers ranging from maladaptation to inviability or sterility in the offspring have attracted biologists’attention because of the problem of the evolution of the“unfit”offspring.A clear model to solve this problem is the Dobzhansky-Muller(DM)model(Figure 1;Coyne and Orr,2004),in which incompatibilities are generally caused by an interaction between the genes that have functionally diverged in each population without selection opposing any intermediate step(Coyne and Orr,2004).

关键词： Figure Forms breeding

Identification of differentially expressed genes of primary spermatocyte against round spermatid isolated from human testis using the laser capture microdissection technique

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cell Research 2004年第6期14卷 507-512页

作者： S.S.koide 1230 York Avenue Center for Biomedical Research Population Council NY 10021 New York USA

The method of laser capture microdissection (LCM) combined with suppressive subtractive hybridization (SSH) was developed to isolate specific germ cells from human testis sections and to identify the genes expressed during differentiation and development. In the present study, over 10,000 primary spermatocytes and round spermatid cells were successfully isolated by LCM. Using the cDNAs from primary spermatocytes and round spermatids, SSH cDNAs library of primary spermatocyte-specific was constructed. The average insert size of the cDNA isolated from 75 randomly picked white clones was 500 bp, ranging from 250 bp to 1.7 kb. Using the dot-blot method, a total of 421 clones were examined, resulting in the identification of 390 positive clones emitting strong signals. Partial sequence of cDNAs prepared from each clone was determined with an overall success rate of 84.4%. Genes encoding cytochrome c oxidase II and the rescue factor-humanin were most frequently expressed in primary spermatocytes, suggesting th

关键词： laser capture microdissection suppressive subtractive hybridization spermatogenesis cytochrome c oxidase Ⅱ humanin.

Protein phase separation of plant nuclear pore complex

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2023年第6期16卷 973-974页

作者： Kentaro Tamura Masaya koide School of Food and Nutritional Sciences Department of Environmental and Life SciencesUniversity of ShizuokaShizuoka 422-8526Japan

The nuclear pore complex(NPC)is an evolutionally conserved channel that controls the exchange of macromolecules between the nucleus and *** pores of the NPC form a permeability barrier that allows free permeability of small molecules(<40 kDa or~5 nm in diameter)(Schmidt and Gorlich,2016).However,larger molecules must interact with nuclear transport receptors(NTRs)to overcome the NPC permeability barrier.

关键词： separation overcome pores

Straw return influences the structure and functioning of arbuscular mycorrhizal fungal community in a rice-wheat rotation system

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Pedosphere 2024年第2期34卷 339-350页

作者： Silong ZHAI Junjie XIE Zongyi TONG Bing YANG Weiping CHEN Roger Tkoide Yali MENG Xiaomin HUANG Atta Mohi Ud DIN Changqing CHEN Haishui YANG College of Agriculture Nanjing Agricultural UniversityNanjing 210095China Department of Biology Brigham Young UniversityProvo UT 84602USA Jiangsu Key Laboratory of Crop Genetics and Physiology/Jiangsu Key Laboratory of Crop Cultivation and Physiology Agricultural College of Yangzhou UniversityYangzhou 225009China National Research Center of Intercropping The Islamia University of BahawalpurBahawalpur 63100Pakistan Jiangsu Collaborative Innovation Center for Modern Crop Production Nanjing Agricultural UniversityNanjing 210095China

Straw return is a sustainable soil fertility-building practice,which can affect soil microbial ***,how straw return affects arbuscular mycorrhizal fungi(AMF)is not well ***,we studied the impacts of different straw management treatments over eight years on the structure and functioning of AMF communities in a rice-wheat rotation *** straw management treatments included no tillage with no straw(NTNS),rotary tillage straw return(RTSR),and ditch-buried straw return(DBSR).The community structure of AMF was characterized using high-throughput sequencing,and the mycorrhizal functioning was quantified using an in situ mycorrhizal-suppression *** straw management treatments formed unique AMF community structure,which was closely related to changes in soil total organic carbon,available phosphorus,total nitrogen,ammonium,and *** compared with NTNS,RTSR significantly increased Shannon diversity in 0–10 cm soil layer,while DBSR increased it in 10–20 cm soil layer;DBSR significantly increased hyphal length density in the whole ploughing layer(0–20 cm),but RTSR only increased it in the subsurface soil layer(10–20 cm).The mycorrhizal responses of shoot biomass and nutrient(N and P)uptake were positive under both straw return treatments(RTSR and DBSR),but negative under *** community composition of AMF was significantly correlated to hyphal length density,and the latter was further a positive predictor for the mycorrhizal responses of plant growth and nutrient *** findings suggest that straw return can affect AMF community structure and functioning,and farmers should manage mycorrhizas to strengthen their beneficial effects on crop production.

关键词： community structure hyphal length density shoot biomass shoot N uptake shoot P uptake soil physicochemical properties straw management tillage

维普期刊数据库

Assessing the supercooling of fresh-cut onions at−5℃ using electrical impedance analysis

在线全文

学校读者我要写书评

暂无评论

Food Quality and Safety 2020年第2期4卷 55-58页

作者： Shoji koide Ami Yoneyama Takahiro Orikasa Matsuo Uemura Faculty of Agriculture Iwate UniversityMoriokaJapan Iwate Agri-Innovation Center Iwate UniversityMoriokaJapan

We supercooled fresh-cut onion at−5℃ for 12 *** supercooling,the electric impedance properties of the samples were evaluated by electrical impedance spectroscopy over the frequency range of 42 Hz−5 *** time-temperature profiles of samples indicated that the freezing point and supercooling point were−2.3℃±0.7℃ and−6.9℃±1.0℃,*** results indicated that 34 of the 36 supercooled samples exhibited a definite circular arc in the Cole-Cole plot,which suggested that the cell membrane remained intact during *** the other two samples which did not exhibit a definite circular arc,the cell membrane had sustained serious damage during ***,there was large difference in drip loss percentage between supercooled samples exhibited a definite circular arc in the Cole-Cole plot and samples not exhibiting a definite circular *** results suggest that fresh-cut onions can be supercooled at−5℃.

关键词： supercooling fresh-cut onion electrical impedance Cole-Cole plot supercooling point

Surgical Closure of Coronary Cameral Fistula Draining into the Left Ventricle via a “Fistula Lake”

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Cardiovascular Diseases 2020年第8期10卷 545-549页

作者： Yuchen Cao Masaaki koide yoshifumi Kunii Minori Tateishi Kazumasa Watanabe Satoshi Okugi Yosuke Sakurai Risa Shimbori Division of Cardiovascular Surgery Seirei Hamamatsu General Hospital Hamamatsu Japan

A left-sided lesion of a coronary cameral fistula (CCF) is extremely rare. Surgical closure of the fistula is indicated when symptoms emerge or as a preventive strategy, while surgical approaches depend on the individual anatomical structures. In particular, a CCF forming a “fistula lake” with multiple inflow vessels is so unique that few studies have focused on the technique to close it. We report the successful management of a CCF originating from multiple coronary arteries and draining into the left ventricle via a “fistula lake” by ligation and clipping of associated communication tracts. On the postoperative coronary computed tomography angiography, the fistula lake and the small vessels entering it had all disappeared.

关键词： Coronary Cameral Fistula Fistula Lake Surgical Repair

Impact of blanching pretreatment on the drying rate and energy consumption during far-infrared drying of Paprika(Capsicum annuum L.)

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Food Quality and Safety 2018年第2期2卷 97-103页

作者： Takahiro Orikasa Naoki Ono Takashi Watanabe Yasumasa Ando Takeo Shiina Shoji koide Faculty of Agriculture Iwate UniversityMorioka IARC Kenpoku Agricultural Research Institute KunoheIwate Food Research Institute NAROTsukubaIbaraki Graduate School of Horticulture Chiba UniversityMatsudoJapan

We incorporated a superheated steam blanching pretreatment step into a paprika drying process and compared the far-infrared(FIR)drying rates,hardness of the sample surfaces,cell membrane stabilities,and energy consumption of blanched and non-blanched *** average drying rate of blanched paprika samples during FIR drying was higher than that of non-blanched *** hardness and cell membrane stability of dried blanched samples were lower than those of non-blanched *** estimated that the softening of the sample surfaces and injury to the cell membranes caused the drying rate to *** total energy consumption of the FIR drying of paprika was reduced by approximately 30%by introducing the blanching *** findings contribute to the development of environmentally friendly FIR drying techniques for paprika.

关键词： blanching cell membrane stability,energy consumption far-infrared drying hardness.

Effect of Clostridium butyricum on fecal flora in Helicobacter pylori eradication therapy

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2005年第47期11卷 7520-7524页

作者： Izumi Shimbo Taketo Yamaguchi Takeo Odaka Kenichi Nakajima Akinori koide Hidehiko Koyama Hiromitsu Saisho Department of Medicine and Clinical Oncology Graduate School of MedicineChiba University1-8-1 InohanaChuo-kuChiba-CityChiba260-0856Japan Tako Chuo Hospital Tako-machi Katori-CountryChiba289-2241Japan Kawasaki Chiba Hospital Minami-choChuo-kuChiba-CityChiba260-0842Japan International University of Health and Welfare Mita HospitalMita Minato-ku Tokyo108-8329Japan

AIM： To investigate the effect of probiotic bacterium, Clostridium butyricum MIYAIRI 588 strain （CBM） on the changes of the fecal flora in Helicobacter pylori （H pylon） treatment. METHODS： Thirty-five patients with gastric or duodenal ulcers positive for H pylori were randomized either to 1 wk amoxicillin, clarithromycin, lansoprazole （Group 1） or to the same regimen supplemented with CBM 7 d ahead of the triple therapy （Group 2）. Stool samples were collected before and 2, 4, 7, 15, and 22 d after the starting eradication therapy, and were examined intestinal flora. Patients were required to keep a diary record of their condition. RESULTS： Obligate anaerobes decreased significantly on d 2, 4, 8 and 15 in Group 1. On the other hand, they did not decrease significantly in Group 2. The Escherichia coli was dominant bacterium in Enterobacteriaceae, but that was replaced by other species such as Klebsiella and Enterobacter after eradication in Group 1. The change was suppressed in Group 2. Abdominal symptoms were less frequent in Group 2 than in Group 1. CONCLUSION： The combined use of CBM reduced the changes in the intestinal flora and decreased the incidence of gastrointestinal side effects.

关键词： Clostridium butyricum Intestinal flora Helicobacter pylori Eradication

Cloning of rat sp56, the homologue of mouse sperm ZP3 receptor-sp56

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cell Research 2003年第2期13卷 121-129页

作者： SS koide The Population Council Center for Biomedical Research 1230 York Avenue New York New York 100021 USA

Mouse sp56 is considered as one of the candidates for mouse zona pellucida 3 (mZP3) receptor. Up to date, its homologue has only been cloned from guinea pig. namely AM67. Based on the cDNA sequence of mouse sp56, we designed a pair of primer to amplify its homologue from rat testis cDNA. Using RT-PCR, two fragments of 743 bp and 938 bp were amplified. The PCR products show very high homology to mouse sp56. However, the 743 bp product completely lacks one of the seven Sushi domains of mouse sp56. Using the 743 bp product as the probe to detect the expression profile of sp56 in rat tissues, Northern blot shows that a -2.0 kb mRNA expresses specifically in testis. Employed the RACE method, two full cDNA sequences of rat sp56 were obtained. A Mr -42 KD band was detected in denatured and non-reducing protein sample of rat testis and sperm with anti-mouse sp56 monoclonal antibody by Western blot method. Rat sp56 was localized on rat sperm head by the indirect immunofluorescence method. Rat sp56 immunoreactivi

关键词： ZP3 receptor sp56 rat testis sperm cloning.