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Lipase and photodecarboxylase coexpression: A potential strategy for alkane-based biodiesel production from natural triglycerides

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Chinese Journal of Chemical Engineering 2024年第3期67卷 238-246页

作者： yong-yi zeng Xin-yi Xu Jin-Xuan Xie Wen-Li Chen Lan Liu Xin-Jian yin Bi-Shuang Chen School of Marine Sciences Sun Yat-Sen UniversityZhuhai 519080China Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai) Zhuhai 519080China

Alkane-based biodiesel is considered the next generation of biodiesel owing to its potential environmental benefits and the fact that it exhibits much higher specific caloric values than traditional ***,the formidable obstacle impeding the commercialization of this cutting-edge fuel alternative lies in the cost associated with its *** this study,an engineered strain Escherichia coli(***)showcasing harmonized coexpression of a lipase(from Thermomyces lanuginosus lipase,TLL)and a fatty acid photodecarboxylase(from Chlorella variabilis,CvFAP)was first constructed to transform triglycerides into *** potential of *** BL21(DE3)/pRSFDuet-1-TLL-CvFAP for alkane synthesis was evaluated with tripalmitin as a model substrate under various process *** a comprehensive examination of the reaction parameters,the scope of the biotransformation was expanded to‘real’substrates(vegetable oils).The results showed that bioderived oils can be transformed into alkanes with high yields(0.80-10.20 mmol·L^(-1))under mild conditions(35℃,pH 8.0,and 36 h)and blue light *** selected processes were performed on an increased lab scale(up to 100 ml)with up to 24.77 mmol·L^(-1) tripalmitin,leading to a yield of 18.89 mmol·L^(-1) *** the employment of a method for efficiently producing alkanes under mild conditions and a simple procedure to isolate alkanes from the reaction system,the utilization of sustainable biomass as a fundamental feedstock emerges as the primary solution to lower the cost of alkane-based ***,this study proposes a readily implementable and highly effective approach for alkane-based biodiesel production.

关键词： Alkane-based biodiesel Renewable biomass Co-overexpression Lipase Photodecarboxylase

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Novel model combining contrast-enhanced ultrasound with serology predicts hepatocellular carcinoma recurrence after hepatectomy

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World Journal of Clinical Cases 2021年第24期9卷 7009-7021页

作者： Hai-Bin Tu Li-Hong Chen Yu-Jie Huang Si-yi Feng Jian-Ling Lin yong-yi zeng Department of Ultrasound Mengchao Hepatobiliary Hospital of Fujian Medical UniversityFuzhou 350025Fujian ProvinceChina Department of Hepatobiliary Surgery Mengchao Hepatobiliary Hospital of Fujian Medical UniversityFuzhou 350025Fujian ProvinceChina

BACKGROUND Surgery is the primary curative option in patients with hepatocellular carcinoma(HCC).However,recurrence within 2 years is observed in 30%–50%of patients,being a major cause of *** To construct and verify a non-invasive prediction model combining contrastenhanced ultrasound(CEUS)with serology biomarkers to predict the early recurrence of *** Records of 744 consecutive patients undergoing first-line curative surgery for HCC in one institution from 2016–2018 were reviewed,and 292 local patients were selected for *** characteristics including gender and age,CEUS liver imaging reporting and data system(LIRADS)parameters including wash-in time,wash-in type,wash-out time,and wash-out type,and serology biomarkers including alanine aminotransferase,aspartate aminotransferase,platelets,and alpha-fetoprotein(AFP)were *** analysis and multivariate Cox proportional hazards regression model were used to evaluate the independent prognostic factors for tumor *** a nomogram called CEUS model was *** CEUS model was then used to predict recurrence at 6 mo,12 mo,and 24 mo,the cut-off value was calculate by X-tile,and each C-index was *** Kaplan-Meier curve was compared by logrank *** calibration curves of each time were *** A nomogram predicting early recurrence(ER),named CEUS model,was formulated based on the results of the multivariate Cox regression *** nomogram incorporated tumor diameter,preoperative AFP level,and LIRADS,and the hazard ratio was 1.123(95%confidence interval[CI]:1.041-1.211),1.547(95%CI:1.245-1.922),and 1.428(95%CI:1.059-1.925),*** cut-off value at 6 mo,12 mo,and 24 mo was 100,80,and 50,and the C-index was 0.748(95%CI:0.683-0.813),0.762(95%CI:0.704-0.820),and 0.762(95%CI:0.706-0.819),*** model showed satisfactory results,and the calibration at 6 mo was desirable;however,the calibration at 12 and 24 mo should be improv

关键词： Hepatocellular carcinoma Recurrence Prediction Contrast-enhanced ultrasound Liver imaging reporting and data system Alpha-fetoprotein

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Prevalence,patterns,risk factors and outcomes of peritoneal metastases after laparoscopic hepatectomy for hepatocellular carcinoma:a multicenter study from China

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Hepatobiliary Surgery and Nutrition 2024年第1期13卷 3-15,I0001-I0004页

作者： Yuan-Xiang Lu Jian-Ping Zhao Guan-Dou Yuan Ming-Gen Hu Chuan-Dong Sun Kun-Lun Chen Yao Chen yong-yi zeng Zhi-ying Yang Wan-Guang Zhang Hepatic Surgery Center Tongji Hospital of Tongji Medical College of Huazhong University of Science and TechnologyWuhanChina Department of Hepatobiliary Surgery The First Affiliated Hospital of Guangxi Medical UniversityNanningChina Faculty of Hepato-Pancreato-Biliary Surgery Chinese PLA General HospitalBeijingChina Department of Hepatobiliary and Pancreatic Surgery The Affiliated Hospital of Qingdao UniversityQingdaoChina Department of Hepatobiliary and Pancreatic Surgery The First Affiliated Hospital of Zhengzhou UniversityZhengzhouChina Department of Radiology Intervention The Affiliated Tumor Hospital of Zhengzhou UniversityHenan Cancer HospitalZhengzhouChina Department of Hepatobiliary Surgery Mengchao Hepatobiliary Hospital of Fujian Medical UniversityFuzhouChina Department of General Surgery China-Japan Friendship HospitalBeijingChina

Background:We aim to investigate the prevalence,patterns,risk factors,and outcomes of peritoneal metastases(PM)after curative laparoscopic hepatectomy(LH)for hepatocellular carcinoma(HCC).Methods:A multicenter cohort of 2,138 HCC patients who underwent curative LH from August 2010 to December 2016 from seven hospitals in China was retrospectively *** incidence of PM following LH was evaluated and compared with that in open hepatectomy(OH)after 1:1 propensity score matching(PSM).Results:PM prevalence was 5.1%(15/295)in the early period[2010-2013],2.6%(47/1,843)in the later period[2014-2016],and 2.9%(62/2,138)in all LH patients,which was similar to 4.0%(59/1,490)in the OH *** recurrence patterns,timing,and treatment did not significantly vary between the LH and OH patients(P>0.05).Multivariate logistic regression revealed that tumor diameter>5 cm,non-anatomical resection,presence of microvascular invasion,and lesions<2 cm from major blood vessels were independent risk factors of PM after *** the 62 cases with PM,26(41.9%)had PM only,34(54.9%)had intrahepatic recurrence(IHR)and PM,and 2(3.2%)had synchronous extraperitoneal metastases(EPM).Patients with resectable PM had a 5-year overall survival(OS)of 65.0%compared to 9.0%for unresectable PM(P=0.001).Conclusions:The prevalence,patterns and independent risk factors of PM were identified for HCC patients after *** was not associated with increased incidence of PM in HCC patients for experienced *** re-excision of PM was associated with prolonged survival.

关键词： Prevalence hepatocellular carcinoma(HCC) peritoneal metastases(PM) laparoscopic hepatectomy(LH) open hepatectomy(OH)

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Grading severity of microscopic vascular invasion was independently associated with recurrence and survival following hepatectomy for solitary hepatocellular carcinoma

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Hepatobiliary Surgery and Nutrition 2024年第1期13卷 16-28页

作者： Lan-Qing Yao Chao Li yong-Kang Diao Lei Liang Hang-Dong Jia Shi-Chuan Tang yong-yi zeng Han Wu Ming-Da Wang Li-Hui Gu Timothy M.Pawlik Wan Yee Lau Cheng-Wu Zhang Feng Shen Kui Wang Tian Yang Department of Hepatobiliary Surgery Eastern Hepatobiliary Surgery HospitalSecond Military Medical University(Navy Medical University)ShanghaiChina Department of General Surgery Cancer CenterDivision of Hepatobiliary and Pancreatic SurgeryZhejiang Provincial People’s HospitalAffiliated People’s HospitalHangzhou Medical CollegeHangzhouChina Department of Hepatobiliary Surgery Mengchao Hepatobiliary HospitalFujian Medical UniversityFuzhouChina Department of Surgery Ohio State UniversityWexner Medical CenterColumbusOHUSA Faculty of Medicine the Chinese University of Hong KongShatinNew TerritoriesHong KongChina Eastern Hepatobiliary Clinical Research Institute Third Affiliated Hospital of Navy Medical UniversityShanghaiChina

Background:Hepatectomy is the preferred treatment for solitary hepatocellular carcinoma(HCC)without macrovascular invasion and distant metastasis,but long-term survival remains unsatisfactory in certain *** sought to identify whether the grading severity of microscopic vascular invasion(MVI)was associated with recurrence and survival among patients with solitary ***:Consecutive patients who underwent hepatectomy for solitary HCC were identified from a multicenter prospectively-collected *** were categorized into three groups according to the MVI grading system proposed by the Liver Cancer Pathology Group of China:M0(no MVI),M1(1-5 sites of MVI occurring≤1.0 cm away from the tumor),and M2(>5 sites occurring≤1.0 cm or any site occurring>1 cm away from the tumor).Recurrence-free survival(RFS)and overall survival(OS)were compared among the ***:Among 227 patients,97(42.7%),83(36.6%),and 47(20.7%)patients had M0,M1,and M2,*** RFS rates among patients with M0,M1,and M2 were 38.3,35.1,11.6 months,respectively,while OS rates were 66.8,62.3,30.6 months,respectively(both P<0.001).Multivariate Cox-regression analyses demonstrated that both M1 and M2 were independent risk factors for RFS(hazard ratio 1.20,95%CI:1.03-1.89,P=0.040;and hazard ratio 1.67,95%CI:1.06-2.64,P=0.027)and OS(hazard ratio 1.28,95%CI:1.05-2.07,P=0.035;and hazard ratio 1.97,95%CI:1.15-3.38,P=0.013).Conclusions:Grading severity of MVI was independently associated with RFS and OS after hepatectomy for solitary *** surveillance for recurrence and potentially adjuvant therapy may be considered for patients with MVI,especially individuals with more severe MVI grading(M2).

关键词： Hepatocellular carcinoma microscopic vascular invasion recurrence survival hepatectomy

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Diagnosis and Treatment of Cholangiocarcinoma: A Consensus from Surgical Specialists of China

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2014年第4期34卷 469-475页

作者： Jian-qiang Cai Shou-wang Cai Wen-ming Cong Min-shan Chen Ping Chen Xiao-ping Chen Yan-ling Chen yi-fa Chen Chao-liu Dai Qiang Huang Zhi-yong Huang Bo Jiang Bin Jiang Ke-wei Jiang Bo Li Zong-fangLi Li-jian Liang Bin Liu Hui-chun Liu Lian-xin Liu Qing-guang Liu Rong Liu ying-bin Liu Jian-guo Lu Shi-chun Lu yi Lu yi-lei Mao Bin Mei Jun Niu Bao-gang Peng Xiao Qin Yu-dong Qiu Guang-yi Wang Yao-don Wangg Zhi-ming Wang Ren-hua Wan Ya-fu Wu Bao-cai Xing Feng Xia Ge-liang Xu Jia-mei Yang Xiao-fang Yu yong zeng yong-yi zeng Bi-xiang Zhang Bin-hao Zhang Qi-yu Zhang Shui-jun Zhang Wan-guang Zhang yong-jie Zhang Zhi-wei Zhang Dong Zhou Wei-ping Zhou Cancer Institute and Hospital Chinese Academy of Medical Sciences Chinese PLA General Hospital Eastern Hepatobiliary Surgery Hospital of Second Military Medical University Sun Yat-sen University Cancer Center Daping Hospital of Third Military Medical University Tongji Hospital Tongji Medical CollegeHuazhong University of Science and Technology Fujian Medical University Union Hospital Shengjing Hospital of China Medical University Anhui Provincial Hospital Hunan Provincial People’s Hospital Taihe Hospital Affiliated to Hubei University of Medicine Peking University People’s Hospital West China Hospital Sichuan University The Second Affiliated Hospital of Xi’an Jiao Tong University The First Affiliated Hospital Sun Yat-sen University First Affiliated Hospital of Kunming Medical University The First Affiliated Hospital of Bengbu Medical College The First Affiliated Hospital of Harbin Medical University The First Affiliated Hospital of Xi’an Jiao Tong University Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Tangdu Hospital of Fourth Military Medical University Peking Union Medical College Hospital Qilu Hospital of Shandong University The First Affiliated Hospital of Guangxi Medical University Nanjing Drum Tower Hospital The First Affiliated Hospital of Jilin University Fujian Provincial Hospital Xiangya Hospital of Central South University The First Affiliated Hospital of Nanchang University Beijing Cancer Hospital Southwest Hospital of Third Military Medical University Shenzhen People’s Hospital The First Affiliated Hospital of Fujian Medical University The First Affiliated Hospital of Wenzhou Medical University The First Affiliated Hospital of Zhengzhou University Fujian Provincial Cancer Hospital

Cholangiocarcinoma refers to malignant tumors that develop in epithelial lining of biliary system, and it is divided into two categories according to tumor location, intrahepatic cholangiocarcinoma （ICC） and extrahepatic cholangiocarcinoma （ECC）. ICC occurs from the epithelial cells of the intrahepatic bile duct, its branches and interlobular biliary tree; and ECC is divided into hilar cholangiocarcinoma and distal cholangiocarcinoma by the circumscription at the confluence of cystic duct and the common hepatic duct.

关键词： A Consensus from Surgical Specialists of China Diagnosis and Treatment of Cholangiocarcinoma

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Association of Pringle maneuver with postoperative recurrence and survival following hepatectomy for hepatocellular carcinoma: a multicenter propensity score and competing-risks regression analysis

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Hepatobiliary Surgery and Nutrition 2024年第3期13卷 412-424,I0003-I0010页

作者： Shi-Chuan Tang yong-Kang Diao Kong-ying Lin Chao Li Xiao Xu Lei Liang Jie Kong Qing-Jing Chen Xian-Ming Wang Fu-Bao Liu Wei-Min Gu Ya-Hao Zhou ying-Jian Liang Hong-Zhi Liu Ming-Da Wang Lan-Qing Yao Timothy M.Pawlik Feng Shen Wan Yee Lau Tian Yang yong-yi zeng Department of Hepatobiliary Surgery Mengchao Hepatobiliary Hospital of Fujian Medical UniversityFuzhouChina Department of Hepatobiliary Surgery Eastern Hepatobiliary Surgery HospitalNavy Medical University(Second Military Medical University)ShanghaiChina Department of Hepatobiliary Surgery Affiliated Hospital of Nantong UniversityNantongChina Department of Hepatobiliary Surgery People’s Hospital of Zhejiang ProvincialPeople’s Hospital of Hangzhou Medical CollegeHangzhouChina Department of Hepatobiliary Heze Municiple HospitalHezeChina Department of General Surgery First Affiliated Hospital of Shandong First Medical University&Shandong Provincial Qianfoshan HospitalJinanChina Department of Hepatobiliary Surgery First Affiliated Hospital of Anhui Medical UniversityHefeiChina The First Department of General Surgery Fourth Hospital of HarbinHarbinChina Department of Hepatobiliary Surgery Pu’er People’s HospitalPu’erChina Department of Hepatobiliary Surgery First Affiliated Hospital of Harbin Medical UniversityHarbinChina Department of Surgery Ohio State UniversityWexner Medical CenterColumbusOHUSA Faculty of Medicine The Chinese University of Hong KongHong KongChina Department of Hepatopancreatobiliary Surgery First Affiliated Hospital of Fujian Medical UniversityFuzhouChina The Liver Disease Research Center of Fujian Province FuzhouChina

Background:The application of Pringle maneuver(PM)during hepatectomy reduces intraoperative blood loss and the need for perioperative transfusion,but its effect on long-term recurrence and survival for patients with hepatocellular carcinoma(HCC)remains *** sought to determine the association between the application of PM and post-hepatectomy oncologic outcomes for patients with ***:Patients who underwent curative hepatectomy for HCC at 9 Chinese hospitals from January 2010 to December 2018 were *** two propensity score methods[propensity score matching(PSM)and inverse probability of treatment weight(IPTW)],cumulative recurrence rate and cancer-specific mortality(CSM)were compared between the patients in the PM and non-PM *** competing-risks regression models were performed to adjust for the effect of non-cancer-specific mortality and other prognostic risk ***:Of the 2,798 included patients,2,404 and 394 did and did not adopt PM(the PM and non-PM groups),*** rates of intraoperative blood transfusion,postoperative 30-day mortality and morbidity were comparable between the two groups(all P>0.05).In the PSM cohort by the 1:3 ratio,compared to 382 patients in the non-PM group,1,146 patients in the PM group also had the higher cumulative 5-year recurrence rate and CSM(63.9%and 39.1%vs.55.3%and 31.6%,both Pyielded in the entire cohort and the IPTW *** competing-risks regression analyses demonstrated that no application of the PM was independently associated with lower recurrence rate and CSM based on various analytical cohorts[hazard ratio(HR),0.82 and 0.77 in the adjusted entire cohort,HR 0.80 and 0.73 in the PSM cohort,and HR 0.80 and 0.76 in the IPTW cohort,respectively].Conclusions:The findings suggested that no application of PM during hepatectomy for patients with HCC reduced the risk of postoperative recurrence and cancer-specific death by appr

关键词： Hepatocellular carcinoma(HCC) Pringle maneuver(PM) recurrence survival hepatectomy

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In Vitro Cultivation of Cells from Cercariae of Schistosoma japonicum

In Vitro Cultivation of Cells from Cercariae of Schistosoma ...

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中国动物学会第八次全国寄生虫学学术讨论会

作者： ZHANG Zhong-yong, yi Xin-yuan, zeng Xian-fang, LI Jing-ruLaboratory of Schistosomiasis, Xiangya Medical Collage,Central South University Changsha 410078, China

＜正＞ The ability to propagate parasites cells in vitro has proven to be a boon for studies in molecular、cellular、and developmental biology,pharmacology、immunology,and other areas,yet it has remeined aninsurmountable problem for certain *** parasites,many of which require more than onehost species for the completion of their life cycles,are particularly difficult in this *** on thesuccessful establishment of Cysticerous cellulosae cell line,we have recently made an effort to explore thepossibility of in vitro cultivation of Schistosoma japonicum cercaria *** are now reporting thepreliminary success in this regard.

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Surface embedded binary Co-Cu hydroxy phosphate over surface of oxy/hydroxides for efficient nitrate reduction to ammonia

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Nano Research 2025年第1期18卷 195-207页

作者： Chun Zhu yifei Yang yike Jing Zhen zeng Xuesong yi Wei Sun Fei Yang Key Laboratory of Agro-Forestry Environmental Processes and Ecological Regulation of Hainan Province School of Environmental Science and EngineeringHainan UniversityHaikou 570228China

Electrochemical nitrate reduction reaction(NO3RR)to ammonia is a promising approach to address excess Ncontamination in aqueous environment but suffers from a limited ammonia yield and selectivity due to the low concentration and sluggish *** work reports a three-dimensional porous electrode with binary Co_(2)Cu_(1)hydroxy phosphate embedded into the surface of Ni_(3)Co_(1)oxy/hydroxides(CoCu-PH@NiCo/Ni,“PH”means phosphate-hydroxide),which delivers a yield of 4.13 mg·h^(-1)·cm^(-2)with a high Faraday efficiency of 92.6%under a low nitrate concentration of 40 mM and provide an impressive *** embedded structure of the as-prepared catalyst exhibits unique metal coordination ligands and valence states different from the unitary *** electrochemical in situ infrared spectra and theoretical calculations revealed that the Co sites with the ability to dissociate water molecular to form adsorbed OH*can effectively provide the required protons for the deoxygenation and hydrogenation steps during NO3-conversion,thereby,effectively reducing the energy barrier of the key transition states in the NO3RR process to improve the intrinsic *** demonstrate that the rational design of binary components with the appropriate ability to activate water molecules as well as the threedimensional porous skeletal structure are expected to promote effectively the low-concentration nitrates conversion to ammonia.

关键词： nitrate reduction hydroxy phosphate surface embedded water dissociation active hydroxy adsorbed

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珠江三角州沙湾断裂带第四纪活动性研究

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中国地质 2016年第5期43卷 1803-1813页

作者：董好刚路韬何万双黎义勇曾敏中国地质调查局武汉地质调查中心中国人民解放军66259部队

沙湾断裂是珠江三角州北西向的主干断裂。为了查清其第四纪活动性,我们进行了1∶5万地质地貌填图、断层活动年代测定、氡气测量、浅层地震勘测和联合钻孔验证及构造解析等工作。第四纪地貌与断裂的耦合性及历史地震情况说明,沙湾断裂目... 详细信息

沙湾断裂是珠江三角州北西向的主干断裂。为了查清其第四纪活动性,我们进行了1∶5万地质地貌填图、断层活动年代测定、氡气测量、浅层地震勘测和联合钻孔验证及构造解析等工作。第四纪地貌与断裂的耦合性及历史地震情况说明,沙湾断裂目前仍有一定活动性;地质调查及勘测未发现断裂切割第四系,氡气测量结果表明断裂目前活动性中等以下,说明沙湾断裂目前活动性较弱;年代测量结果表明断裂在中更新世中期至晚更新世前期活动性较强,晚更新世以后活动性渐弱,仅在南段南沙区大岗镇罗汉山发现晚于100 ka剖面。罗汉山构造解析实例说明三角洲局部地段仍受断裂活动控制处于拉张环境,但全新世以来活动性渐弱。综合研究认为,沙湾断裂目前仍有一定活动性,但活动性较弱。

关键词：珠江三角洲沙湾断裂带第四纪地貌浅层地震探测年代学第四纪活动性

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Induced pluripotent stem cell-related approaches to generate dopaminergic neurons for Parkinson's disease

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Neural Regeneration Research 2025年第11期20卷 3193-3206页

作者： Ling-Xiao yi Hui Ren Woon Genevieve Saw Li zeng Eng King Tan Zhi Dong Zhou National Neuroscience Institute Singapore Nanyang Technology University Singapore Department of Neurology Singapore General HospitalSingapore Signature Research Program in Neuroscience and Behavioral Disorders Duke-NUS Medical SchoolSingapore

The progressive loss of dopaminergic neurons in affected patient brains is one of the pathological features of Parkinson's disease,the second most common human neurodegenerative *** the detailed pathogenesis accounting for dopaminergic neuron degeneration in Parkinson's disease is still unclear,the advancement of stem cell approaches has shown promise for Parkinson's disease research and *** induced pluripotent stem cells have been commonly used to generate dopaminergic neurons,which has provided valuable insights to improve our understanding of Parkinson's disease pathogenesis and contributed to anti-Parkinson's disease *** current review discusses the practical approaches and potential applications of induced pluripotent stem cell techniques for generating and differentiating dopaminergic neurons from induced pluripotent stem *** benefits of induced pluripotent stem cell-based research are *** dopaminergic neuron differentiation protocols from induced pluripotent stem cells are *** emerging three-dimension-based brain organoid models compared with conventional two-dimensional cell culture are ***,limitations,challenges,and future directions of induced pluripotent stem cell–based approaches are analyzed and proposed,which will be significant to the future application of induced pluripotent stem cell-related techniques for Parkinson's disease.

关键词： dopaminergic neurons induced pluripotent stem cells Parkinson's disease stem cell approaches

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