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Protection of the liver against CCl_4-induced injury by intramuscular electrotransfer of a kallistatin-encoding plasmid

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World Journal of Gastroenterology 2011年第1期17卷 111-117页

作者： yong diao Xiao-Feng Zhao Jun-Sheng Lin Qi-Zhao Wang Rui-An Xu Institute of Molecular Medicine Huaqiao University Quanzhou362021 Fujian Province China Centre for Reproduction and Genomics AgResearch Invermay Mosgiel 9053 New Zealand

AIM:To investigate the effect of transgenic expression of kallistatin(Kal) on carbon tetrachloride(CCl 4)induced liver injury by intramuscular(im) electrotransfer of a Kal-encoding plasmid formulated with poly-Lglutamate(PLG).METHODS:The pKal plasmid encoding Kal gene was formulated with PLG and electrotransferred into mice skeletal muscle before the administration of CCl *** expression level of Kal was *** serum biomarker levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),malonyldialdehyde(MDA),and tumor necrosis factor(TNF)-α were *** extent of CCl 4-induced liver injury was analyzed ***:The transgene of Kal was sufficiently expressed after an im injection of plasmid formulated with PLG followed by *** the Kal gene-transferred mice,protection against CCl 4-induced liver injury was reflected by significantly decreased serum ALT,AST,MDA and TNF-α levels compared to those in control mice(P < 0.01 to 0.05 in a dose-dependent manner).Histological observations also revealed that hepatocyte necrosis,hemorrhage,vacuolar change and hydropic degeneration were apparent in mice after CCl 4 *** contrast,the damage was markedly attenuated in the Kal gene-transferred *** expression of hepatic fibrogenesis marker transforming growth factor-β1 was also reduced in the pKal transferred ***:Intramuscular electrotransfer of plasmid pKal which was formulated with PLG significantly alleviated the CCl 4-induced oxidative stress and inflammatory response,and reduced the liver damage in a mouse model.

关键词： Kallistatin Gene delivery systems Electroporation Drug formulation Liver injury

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Culturable plant pathogenic fungi associated with sugarcane in southern China

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Fungal Diversity 2019年第6期99卷 1-104页

作者： Mubashar Raza Zhi-Feng Zhang Kevin D.Hyde yong-Zhao diao Lei Cai State Key Laboratory of Mycology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China Center of Excellence in Fungal Research Mae Fah Luang UniversityChiang Rai 57100Thailand College of Life Science University of Chinese Academy of SciencesBeijing 100049China

Sugarcane(Saccharum officinarum)is one of the oldest crops cultivated by *** fungal taxa have been reported from this host,although most were not identified beyond genus *** this study,we explored 31 sampling sites in Guangxi and Guangdong Provinces(China),and collected 370 diseased samples from leaves and roots of sugarcane,from which 762 strains were *** preliminary analysis based on internal transcribed spacer(ITS)sequences suggested that these isolates belonged to 143 species in 51 genera,but we could not assign 129 ***,Chaetomium,Curvularia,Phoma and Nigrospora represented the top five most common genera identified,while 27 rare genera comprised only one identified *** this study,we chose above mentioned common genera for in-depth morphological observations and multi-locus analyses,in order to identify these strains to species *** this paper,we described one new genus,32 new species,and reported 19 new records for China and the asexual morph of Chaetomium ***,this is the most comprehensive study with molecular identification and illustration of fungi associated with sugarcane,which greatly improves our understanding of culturable mycota associated with this host.

关键词： 33 new taxa Multi-locus phylogeny Pathogenic fungi Sugarcane diseases Taxonomy

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Associations between the use of red yeast rice preparations and adverse health outcomes:An umbrella review of meta-analyses of randomized controlled trials

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Journal of Integrative Medicine 2024年第2期22卷 126-136页

作者： Zhen-yu Ma Shu-ping Yang Ying Li Tian-tian Xu Ya-lin Yang Hui-yong Yang Heng-bing Li Le-jin Zhou yong diao Su-yun Li School of Medicine Huaqiao UniversityQuanzhou 362021Fujian ProvinceChina College of Traditional Chinese Medicine Shandong University of Traditional Chinese MedicineJinan 250355Shandong ProvinceChina Second Clinical Medical College Nanjing Medical UniversityNanjing 211166Jiangsu ProvinceChina

Background:Red yeast rice(RYR),a natural lipid-lowering agent,is widely used in clinical ***,the existing meta-analyses concerning the safety of RYR preparations have yielded inconsistent results,and the credibility of the evidence has not been ***:This study was designed to evaluate the existing evidence and offer a comprehensive understanding of the associations between the use of RYR preparations and various adverse health *** strategy:Seven literature databases were searched from inception to May 5,2023,using medical subject headings and free-text terms(e.g.,“red yeast rice,”“Xuezhikang,”and“Zhibitai”).Inclusion criteria:Meta-analyses that investigated and quantitatively estimated associations between the use of RYR preparations and adverse health outcomes were included in this *** extraction and analysis:Two researchers independently extracted data using a standardized data collection table;any disagreements were resolved by consulting a third *** on the participant,intervention,comparator and outcome(PICO)framework in each eligible meta-analysis,a series of unique associations between the use of RYR preparations and adverse health outcomes were *** associations’effect estimates were re-evaluated using random-effect ***:Fifteen meta-analyses,comprising 186(164 unique)randomized controlled trials,were *** on A Mea Surement Tool to Assess Systematic Reviews version 2,3(20%)and 12(80%)of these meta-analyses had low and critically low confidence,respectively.A total of 61 unique associations between the use of RYR preparations and adverse health outcomes were extracted from eligible *** on the random-effect models,10(16.4%)associations indicated a significant protective effect of RYR preparations against adverse health outcomes,while 5(8.2%)indicated an increased risk of adverse health outcomes related to uric acid,alanine transaminase and aspartate trans

关键词： Red yeast rice Safety Adverse health outcomes Umbrella review Meta-analysis Systematic review

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Clinical evaluation of sintilimab in conjunction with bevacizumab for advanced colorectal cancer with microsatellite stable-type after failure of first-line therapy

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World Journal of Gastrointestinal Surgery 2024年第10期16卷 3277-3287页

作者： Liang Wang yong-Zhi diao Xin-Fu Ma Yu-Shuang Luo Qi-Jing Guo Xiao-Qian Chen Department of Gastrointestinal Oncology Surgery Affiliated Hospital of Qinghai UniversityXining 810000Qinghai ProvinceChina Department of Gastroenterology The First People’s Hospital of XiningXining 810000Qinghai ProvinceChina Department of Medical Oncology Affiliated Hospital of Qinghai UniversityXining 810000Qinghai ProvinceChina

BACKGROUND At present,immune checkpoint inhibitors(ICIs)remain the 1st-line therapy me-thod for patients suffering from high microsatellite instability/deficient misma-tch repair metastatic colorectal cancer(mCRC).However,ICI treatments demon-strate minimal therapeutic efficacy against microsatellite stable(MSS)/proficient mismatch repair(pMMR)*** is mainly because this type of tumor is a“cold tumor”with almost no lymphocyte ***-angiogenic drugs have been found to improve the immune microenvironment by promoting many immune cells to enter the immune microenvironment,thereby exerting anti-tumor *** To investigate the effects of ICIs combined with bevacizumab monoclonal anti-body on tumor immune cells in MSS/pMMR advanced CRC patients with first-line treatment *** A total of 110 MSS/pMMR patients with advanced CRC after first-line treatment failure in the Affiliated Hospital of Qinghai University were enrolled for a ran-domized controlled *** short,patients in the experimental group(n=60)were given sintilimab plus bevacizumab for 4 cycles,and those in the control group(n=50)patients were treated with FOLFIRI combined with bevacizumab for 4 *** expression levels of cluster of differentiation(CD)8(+)T cells,tumor-associated macrophages(TAMs),and cancer-associated fibroblasts(CAFs)were comprehensively evaluated to assess the effects of sintilimab combined with bevacizumab on MSS/pMMR advanced CRC sufferers following failure of 1st-line *** The positive expression rates of CD8(+)T lymphocytes(30%vs 50%),TAMs(23.30%vs 60%),and CAFs(23.30%vs 50%)before and after treatment in both groups exhibited statistical significance(P<0.05).Additionally,the therapeutic effects of both groups(partial remission:26.67%vs 10%;objective response rate:26.70%vs 10%)were significantly different(P<0.05).Although the experimental group showed a higher progression-free survival,median progression-free survival,and disease control rate

关键词： Immune checkpoint inhibitors Bevacizumab Colorectal cancer Cytotoxic T lymphocytes Tumor-associated macrophages Cancer-associated fibroblasts

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基于模糊Petri网的三值产生式知识表示及推理

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模式识别与人工智能 2005年第3期18卷 340-344页

作者：刁永锋陈星西华师范大学计算机应用研究所南充637002

针对传统产生式规则无法进行不确定性知识表示和推理的局限,本文使用三值产生式规则,用-1表示前提、结论和它们之间的不确定性,提出一种基于模糊Petri网的三值产生式知识表示和不确定性推理算法。该算法充分利用推理过程中已得到的中间... 详细信息

针对传统产生式规则无法进行不确定性知识表示和推理的局限,本文使用三值产生式规则,用-1表示前提、结论和它们之间的不确定性,提出一种基于模糊Petri网的三值产生式知识表示和不确定性推理算法。该算法充分利用推理过程中已得到的中间结论,通过标识和关联矩阵的运算实现高速推理。

关键词：模糊Petri网多值逻辑知识表示不确定性推理

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仅基于RNA元件构建可诱导哺乳动物细胞基因表达的调控系统

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中国生物工程杂志 2011年第12期31卷 120-125页

作者：刁勇华侨大学分子药物学研究所泉州362021 分子药物教育部工程研究中心泉州362021

大量的临床前和临床研究结果已表明基因治疗是理想的疾病治疗手段,然而如何实现治疗基因表达的精确调控仍然是研究人员面临的主要挑战。目前临床前研究常用的基因调控系统多基于控制转录,对反式转录激活因子和专门启动子元件的依赖限制... 详细信息

大量的临床前和临床研究结果已表明基因治疗是理想的疾病治疗手段,然而如何实现治疗基因表达的精确调控仍然是研究人员面临的主要挑战。目前临床前研究常用的基因调控系统多基于控制转录,对反式转录激活因子和专门启动子元件的依赖限制了该系统的临床应用。最近,仅采用RNA元件构建的几种基因表达调控系统得到开发,其作用机制为核酶介导的RNA自我切割、RNA干扰、mRNA翻译启动或终止控制等。该类系统的调控活性由小分子配体反式控制,诱导基因表达的变化幅度可观,反应快速,在哺乳动物体内外均可实现。该系统结构模块化,调控活性可调节,可以克服现有转录调节系统的一些应用局限,对将来基因治疗的临床应用具有重要意义。

关键词：基因表达基因治疗哺乳动物细胞转录后控制

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Inhibition of angiogenesis and HCT-116 xenograft tumor growth in mice by kallistatin

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World Journal of Gastroenterology 2007年第34期13卷 4615-4619页

作者： yong diao Jian Ma Wei-Dong Xiao Jia Luo Xin-Yan Li Kin-Wah Chu Peter WC Fung Nagy Habib Farzin Farzaneh Rui-An Xu Molecular Medicine Engineering Research Center of the Ministry of Education Institute of Molecular Medicine Huaqiao University Quanzhou362021 Fujian Province China Department of Medicine Hong Kong University Hong Kong China Department of Pharmacy Chicago UniversityUnited States Department of Transplantation Imperial College London United Kingdom Department of Haematological & Molecular Medicine King's College London London SE5 9NUUnited Kingdom

AIM： TO investigate the inhibitory effect of kallistatin （KAL） on angiogenesis and HCT-116 xenograft tumor growth.METHODS： Heterotopic subcutaneous injection of 2 Seven days later, 2 x 1011 injected intratumorally （n tumors were induced by x 106 HCT-11 cells in mice. rAAV-GFP or rAAV-KAL was = 5 for each group）. The mice were sacrificed at d 28, by which time the tumors in the rAAV-GFP group had grown to beyond 5% of the total body weight. Tumor growth was measured by calipers in two dimensions. Tumor angiogenesis was determined with tumor microvessel density （MVD） by immunohistology. Tumor cell proliferation was assessed by Ki-67 staining.RESULTS： Intratumor injection of rAAV-KAL inhibited tumor growth in the treatment group by 78% （171 ＋ 52 mm^3） at d 21 after virus infection compared to the control group （776 ＋ 241 mm^3）. Microvessel density was significantly inhibited in tumor tissues treated with rAAV-KAL, rAAV-KAL also decreased the proportion of proliferating cells （Ki-67 positive cells） in tumors compared with the control group.CONCLUSION： rAAV-mediated expression of KAL inhibits the growth of colon cancer by reducing angiogenesis and proliferation of tumor cells, and may provide a promising anti-angiogenesis-based approach to the treatment of metastatic colorectal cancer.

关键词： Kallistatin Adeno-associated virus Angiogenesis inhibitors Colon Neoplasm

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中等收入群体的基本分析

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北京社会科学 2006年第3期 3-8页

作者：刁永祚首都师范大学法学院北京100037

积极扩大中等收入群体比重,是党的十六大提出的重要目标,是构建和谐社会的重要内容,也是建设现代社会的重要标志,对经济和社会发展意义重大。中等收入群体在不同的社会和同一社会发展的不同阶段,其界定的标准存在较大的差异,具有相对性... 详细信息

积极扩大中等收入群体比重,是党的十六大提出的重要目标,是构建和谐社会的重要内容,也是建设现代社会的重要标志,对经济和社会发展意义重大。中等收入群体在不同的社会和同一社会发展的不同阶段,其界定的标准存在较大的差异,具有相对性。科学认识和准确把握中等收入群体的划分原则和现实标准,同样是推进中等收入群体逐步扩大进程中所必需的。

关键词：中等收入现实标准和谐社会

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Nerve Growth Factor Promotes Angiogenesis and Skeletal Muscle Fiber Remodeling in a Murine Model of Hindlimb Ischemia

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Chinese Medical Journal 2016年第3期129卷 313-319页

作者： yong-Peng diao Feng-Kui Cui Sheng Yan Zuo-Guan Chen Li-Shan Lian Li-Long Guo yong-Jun Li Department of Vascular Surgery Beijing Shijitan Hospital Capital Medical University Beijing 100038 China Department of Vascular Surgery Peking Union Medical College Hospital Peking Union Medical College Chinese Academy of Medical Science Beijing 100730 China Department of Vascular Surgery Daqing Oilfield General Hospital Heilongjiang 163001 China

Background： Therapeutic angiogenesis has been shown to promote blood vessel growth and improve tissue *** growth factor （NGF） has been reported to play an important role in both physiological and pathological *** study aimed to investigate the effects of NGF on angiogenesis and skeletal muscle fiber remodeling in a murine model of hindlimb ischemia and study the relationship between NGF and vascular endothelial growth factor （VEGF） in ***： Twenty-four mice were randomly allocated to normal control group （n =6）, blank control group （n =6）, VEGF gene transfection group （n =6）, and NGF gene transfection group （n =6）.The model of left hindlimb ischemia model was established by ligating the femoral ***165 plasmid （125 μg） and NGF plasmid （125 μg） was injected into the ischemic gastrocnemius of mice from VEGF group and NGF group, *** hindlimb function and ischemic damage were assessed with terminal points at 21th day postischemia *** gastrocnemius of four groups was tested by hematoxylin-eosin staining, proliferating cell nuclear antigen and CD34 immunohistochemistry staining, and myosin ATPase *** and VEGF protein expression was detected by enzyme-linked immunosorbent ***： On the 21th day after surgery, the functional assessment score and skeletal muscle atrophy degree of VEGF group and NGF group were significantly lower than those of normal control group and blank control *** endothelial cell proliferation index and the capillary density of VEGF group and NGF group were significantly increased compared with normal control group and blank control group （P 〈 0.05）.The NGF and VEGF protein expression of NGF group showed a significant rise when compared with blank control group （P 〈 0.05）.Similarly, the VEGF protein expression of VEGF group was significantly higher than that of blank control group （P 〈 0.05）, but there was no significant difference of the NGF protein expressi

关键词： Angiogenesis Limb lschemia Muscle Fiber Type Nerve Growth Factor Vascular Endothelial Growth Factor

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互联网自治域名体系的构造及其自主性研究

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深圳大学学报（理工版） 2012年第4期29卷 347-352页

作者：刁玉平廖铭刁永平广东商学院信息学院广州510320 中国移动广州分公司广州510630 中国电信广州研究院广州510630

基于现有域名体系的树形结构,构造自主自治的可扩展域名体系和自治网络系统间的域名解释机制与层次结构.提出一种互联网自治域名体系的构造方法及实现方案,通过概率分析所提方案与现有方案的不同.研究既不需要从其他国家移植现有的根域... 详细信息

基于现有域名体系的树形结构,构造自主自治的可扩展域名体系和自治网络系统间的域名解释机制与层次结构.提出一种互联网自治域名体系的构造方法及实现方案,通过概率分析所提方案与现有方案的不同.研究既不需要从其他国家移植现有的根域名服务器,也无需改变互联网底层支持技术,就可拥有完全独立自主的根域名服务器,实现互联网自治的技术.结果表明,互联网自治域名体系自主性高、可扩展使用、改造代价小,且不改变现有协议和用户使用模式,无过渡期.该技术为以单边行动打破互联网垄断提供了积极的尝试.

关键词：通信技术互联网系统自治系统自治IP 域名系统根域名服务器

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