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Native and engineered exosomes for inflammatory disease

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Nano Research 2023年第5期16卷 6991-7006页

作者： Xiaoyi Ma Bingbing liu Limin Fan yiqiong liu Yuge Zhao Tianbin Ren Yan Li Yongyong Li Shanghai Skin Disease Hospital School of MedicineTongji UniversityShanghai 200092China

Exosomes are extracellular vesicles which carry specific molecular information from donor cells and act as an intercellular communication vehicle,which have emerged as a novel cell-free strategy for the treatment of many diseases including inflammatory ***,rising studies have developed exosome-based strategies for novel inflammation therapy due to their biocompatibility and *** not only use native exosomes as therapeutic agents for inflammation,but also strive to make up for the natural defects of exosomes through engineering methods to improve and update the property of exosomes for enhanced therapeutic *** engineered exosomes can improve cargo-loading efficiency,targeting ability,stability,etc.,to achieve combined and diverse treatment strategies in inflammation ***,a comprehensive overview of the recent advances in application studies of native and engineered exosomes as well as the engineered methods is ***,potential application prospects,possible challenges,the development of clinical researches of exosome treatment strategy are concluded from plentiful examples,which may be able to provide guidance and suggestions for the future research and application of exosomes.

关键词： inflammation exosome engineered exosome exosomes-based therapy

Cellular rejuvenation:molecular mechanisms and potential therapeutic interventions for diseases

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Signal Transduction and Targeted Therapy 2023年第4期8卷 1571-1609页

作者： Shuaifei Ji Mingchen Xiong Huating Chen yiqiong liu Laixian Zhou Yiyue Hong Mengyang Wang Chunming Wang Xiaobing Fu Xiaoyan Sun Research Center for Tissue Repair and Regeneration Affiliated to Medical Innovation Research Department and 4th Medical Center PLA General Hospital and PLA Medical CollegePLA Key Laboratory of Tissue Repair and Regenerative Medicine and Beijing Key Research Laboratory of Skin InjuryRepair and RegenerationResearch Unit of Trauma CareTissue Repair and RegenerationChinese Academy of Medical Sciences2019RU051Beijing 100048P.R.China State Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical SciencesUniversity of MacaoTaipa999078 Macao SARChina

The ageing process is a systemic decline from cellular dysfunction to organ degeneration,with more predisposition to deteriorated *** refers to giving aged cells or organisms more youthful characteristics through various techniques,such as cellular reprogramming and epigenetic *** great leaps in cellular rejuvenation prove that ageing is not a one-way street,and many rejuvenative interventions have emerged to delay and even reverse the ageing *** the mechanism by which roadblocks and signaling inputs influence complex ageing programs is essential for understanding and developing rejuvenative ***,we discuss the intrinsic and extrinsic factors that counteract cell rejuvenation,and the targeted cells and core mechanisms involved in this ***,we critically summarize the latest advances in state-of-art strategies of cellular *** rejuvenation methods also provide insights for treating specific ageing-related diseases,including cellular reprogramming,the removal of senescence cells(SCs)and suppression of senescence-associated secretory phenotype(SASP),metabolic manipulation,stem cells-associated therapy,dietary restriction,immune rejuvenation and heterochronic transplantation,*** potential applications of rejuvenation therapy also extend to cancer ***,we analyze in detail the therapeutic opportunities and challenges of rejuvenation *** rejuvenation interventions will provide further insights into anti-ageing and ageing-related disease treatment in clinical settings.

关键词： therapeutic mechanisms potential

Mutation inε-Sarcoglycan Induces a Myoclonus-Dystonia Syndrome-Like Movement Disorder in Mice

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Neuroscience Bulletin 2021年第3期37卷 311-322页

作者： Jiao Li yiqiong liu Qin Li Xiaolin Huang Dingxi Zhou Hanjian Xu Feng Zhao Xiaoxiao Mi Ruoxu Wang Fan Jia Fuqiang Xu Jing Yang Dong liu Xuliang Deng Yan Zhang State Key Laboratory of Membrane Biology College of Life SciencesPKU-IDG/McGovern Institute for Brain ResearchDepartment of Geriatric DentistryPeking University School and Hospital of StomatologyPeking UniversityBeijing 100871China Wuhan Institute of Physics and Mathematics Chinese Academy of SciencesWuhan 430071China College of Life Sciences Wuhan UniversityWuhan 430027China

Myoclonus dystonia syndrome(MDS)is an inherited movement disorder,and most MDS-related mutations have so far been found in theε-sarcoglycan(SGCE)coding *** generating SGCE-knockout(KO)and human 237 C>T mutation knock-in(KI)mice,we showed here that both KO and KI mice exerted typical movement defects similar to those of MDS *** promoted filopodia development in vitro and inhibited excitatory synapse formation both in vivo and in *** of function of SGCE leading to excessive excitatory synapses that may ultimately contribute to MDS ***,using a zebrafish MDS model,we found that among 1700 screened chemical compounds,Vigabatrin was the most potent in readily reversing MDS symptoms of mouse disease *** study strengthens the notion that mutations of SGCE lead to MDS and most likely,SGCE functions to brake synaptogenesis in the CNS.

关键词： SGCE MDS Filopodia Synapse Excitability

Sebaceous gland reprogramming with a single gene, PPARG, and small molecules

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Signal Transduction and Targeted Therapy 2023年第9期8卷 3913-3916页

作者： yiqiong liu Shuaifei Ji Huanhuan Gao Huating Chen Jiangbing Xiang Shaoyuan Cui Christos CZouboulis Aizhen Cai Xiaobing Fu Xiaoyan Sun Research Center for Tissue Repair and Regeneration affiliated to the Medical Innovation Research Department and 4th Medical Center PLA General Hospital and PLA Medical CollegePLA Key Laboratory of Tissue Repair and Regenerative Medicine and Beijing Key Research Laboratory of Skin InjuryRepairand RegenerationResearch Unit of Trauma CareTissue Repair and RegenerationChinese Academy of Medical Sciences2019RU051100048 BeijingP.R.China Department of Nephrology The First Medical CenterChinese PLA General HospitalState Key Laboratory of Kidney Diseases100048 BeijingChina Departments of Dermatology VenereologyAllergologyand ImmunologyStaedtisches Klinikum DessauBrandenburg Medical School Theodor Fontane and Faculty of Health Sciences BrandenburgDessauGermany Institute of General Surgery Department of General Surgerythe 1st Medical CenterPLA General Hospital28 Fu Xing Road100853 BeijingP.R.China

Dear Editor,Sebaceous glands(SG)contribute to 90%of the skin surface lipid production and play critical roles in regulating proper skin functions including epidermal barrier maintenance,dermal immune responses,and global body anti-microbial activities.1 Reduced sebaceous lipid has been implicated in diverse skin diseases,including scarring alopecia,inflammation,chronic itch,as well as premature aging of the skin.2 Especially,scar repair after deep burns or large-scale skin defect often leads to irreversible loss of *** to lacking SG,the regenerated tissues cannot fully recapitulate the structural and functional integrity of normal ***,generation of expandable SG cells with developmental and lipid-producing potentials represents a promising approach for treating diseased,damaged,or aged skin with better quality of ***,direct cellular reprogramming has been widely used for obtaining difficult-to-maintain human cells by cell type-specific transcription factors(TFs).

关键词： programming maintain irreversible

Enhancement of Aggression Induced by Isolation Rearing is Associated with a Lack of Central Serotonin

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Neuroscience Bulletin 2019年第5期35卷 841-852页

作者： yiqiong liu Yunong Sun Xiaoyan Zhao Ji-Young Kim Lu Luo Qian Wang Xiaolu Meng Yonghui Li Nan Sui Zhou-Feng Chen Chuxiong Pan Liang Li Yan Zhang State Key Laboratory of Membrane Biology College of Life Sciences Peking University Beijing 100871 China Department of Anesthesiology Beijing Tongren Hospital Capital Medical University Beijing 100005 China Department of Anesthesiology Department of Psychiatry Department of Developmental Biology Center for the Study of Itch Washington University School of Medicine Saint Louis MO 63110 USA School of Psychological and Cognitive Sciences Beijing Key Laboratory of Behavior and Mental Health Peking University Beijing 100871 China Beijing Institute for Brain Disorders Beijing 100069 China Key Laboratory of Mental Health Institute of Psychology Chinese Academy of Sciences Beijing 100101 China Hendrix College Conway AR 72032 USA PKU-IDG/McGovern Institute for Brain Research Beijing 100871 China

Isolation rearing(IR) enhances aggressive behavior, and the central serotonin(5-hydroxytryptamine,5-HT) system has been linked to IR-induced ***, whether the alteration of central serotonin is the cause or consequence of enhanced aggression is still unknown. In the present study, using mice deficient in central serotonin Tph2-/-and Lmx1 b-/-, we examined the association between central serotonin and aggression with or without social isolation. We demonstrated that central serotonergic neurons are critical for the enhanced aggression after IR. 5-HT depletion in wild-type mice increased aggression. On the other hand, application of 5-HT in Lmx1 b-/-mice inhibited the enhancement of aggression under social isolation conditions. Dopamine was downregulated in Lmx1 b-/-mice. Similar to 5-HT, L-DOPA decreased aggression in Lmx1 b-/-mice. Our results linkthe serotoninergic system directly to aggression and this may have clinical implications for aggression-related human conditions.

关键词： 5-HT Aggression Social isolation Dopamine Lmx1b

A volar skin excisional wound model for in situ evaluation of multiple-appendage regeneration and innervation

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Burns & Trauma 2023年第1期11卷 560-572页

作者： Huanhuan Gao yiqiong liu Ziwei Shi Hongliang Zhang Mengyang Wang Huating Chen Yan Li Shaifei Ji Jiangbing Xiang Wei Pi Laixian Zhou Yiyue Hong Lu Wu Aizhen Cai Xiaobing Fu Xiaoyan Sun Research Center for Tissue Repair and Regeneration Affiliated to Medical Innovation Research Department and 4^(th)Medical Center PLA General Hospital and PLA Medical CollegePLA Key Laboratory of Tissue Repair and Regenerative Medicine and Beijing Key Research Laboratory of Skin InjuryRepair and RegenerationResearch Unit of Trauma CareTissue Repair and RegenerationChinese Academy of Medical Sciences2019RU051Beijing 100048P.R.China Institute of Chemistry Chinese Academy of Sciences2 Beiyi StreetZhong guan cunBeijing 100190P.R.China Research Institute of General Surgery Department of General Surgerythe First Medical CenterPLA General Hospital28 Fu Xing RoadBeijing 100853P.R.China

Background:Promoting rapid wound healing with functional recovery of all skin appendages is the main goal of regenerative *** far current methodologies,including the commonly used back excisional wound model(BEWM)and paw skin scald wound model,are focused on assessing the regeneration of either hair follicles(HFs)or sweat glands(SwGs).How to achieve de novo appendage regeneration by synchronized evaluation of HFs,SwGs and sebaceous glands(SeGs)is still ***,we developed a volar skin excisional wound model(VEWM)that is suitable for examining cutaneous wound healing with multiple-appendage restoration,as well as innervation,providing a new research paradigm for the perfect regeneration of skin ***:Macroscopic observation,iodine-starch test,morphological staining and qRT-PCR anal-ysis were used to detect the existence of HFs,SwGs,SeGs and distribution of nerve fibres in the volar *** healing process monitoring,HE/Masson staining,fractal analysis and behavioral response assessment were performed to verify that VEWM could mimic the pathological process and outcomes of human scar formation and sensory function ***:HFs are limited to the *** are densely distributed in the footpads,scattered in the *** volar skin is richly *** wound area of the VEWM at 1,3,7 and 10 days after the operation is respectively 89.17%±2.52%,71.72%±3.79%,55.09%±4.94%and 35.74%±4.05%,and the final scar area accounts for 47.80%±6.22%of the initial *** the wound area of BEWM at 1,3,7 and 10 days after the operation are respectively 61.94%±5.34%,51.26%±4.89%,12.63%±2.86%and 6.14%±2.84%,and the final scar area accounts for 4.33%±2.67%of the initial *** analysis of the post-traumatic repair site for VEWM vs human was performed:lacunarity values,0.040±0.012 vs 0.038±0.014;fractal dimen-sion values,1.870±0.237 vs 1.903±*** nerve function of normal skin vs post-traumatic repair site was assessed

关键词： Wound healing Animal model Multiple appendages Innervation Regeneration evaluation Hair follicles Sweat gland Sebaceous gland

Cloning and Prokaryotic Expression of MAPKK kinase Gene from Dunaliella salina

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Cloning and Prokaryotic Expression of MAPKK kinase Gene from...

杜氏盐藻磷脂酶C基因DsPLC的原核表达与纯化

中国生物化学与分子生物学会2016年全国学术会议

作者： Chai Xiaojie liu yiqiong Cong Yuting Wang Yiyun Yue Jinrong Key Laboratory of Hydrobiology in Liaoning Province Dalian Ocean University

A MAPKK kinase gene was firstly cloned from Dunaliela salina by RT-PCR and RACE *** full length of c DNA for MAPKKK gene was 1 460 bp with a 27bp 5’UTR,a 554bp 3’UTR and a 879bp open reading frame coding 292 amino *... 详细信息

关键词： Dunaliella salina MAPKKK gene cloning bioinformatics prokaryotic expression western blotting

来源： cnki会议评论

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核农学报 2016年第9期30卷 1679-1683页

作者：刘丽颖柴晓杰丛玉婷武天祥刘艺琼大连海洋大学/农业部北方海水增养殖重点实验室/辽宁省海洋生物资源恢复与生境修复重点实验室辽宁大连116023

为了进一步研究磷脂酶C的生理功能,利用RT-PCR技术扩增了Ds PLC的开放阅读框序列,并与质粒p GS21a连接,构建了原核表达载体p GS21a-Ds PLC。将该重组质粒导入大肠杆菌BL21(DE3)感受态细胞,经IPTG(异丙基-β-D-硫代半乳糖苷)诱导表达出... 详细信息

为了进一步研究磷脂酶C的生理功能,利用RT-PCR技术扩增了Ds PLC的开放阅读框序列,并与质粒p GS21a连接,构建了原核表达载体p GS21a-Ds PLC。将该重组质粒导入大肠杆菌BL21(DE3)感受态细胞,经IPTG(异丙基-β-D-硫代半乳糖苷)诱导表达出融合蛋白。经SDS-PAGE检测,融合蛋白在包涵体和上清中均存在。可溶性蛋白经His柱纯化后进行电泳分析,结果表明,在96k Da左右有单一的蛋白条带,说明融合蛋白得到有效纯化。蛋白印迹结果显示在96kDa左右有明显的杂交条带,初步证明纯化的蛋白是带有His标签的磷脂酶C。盐藻磷脂酶C的成功表达与纯化,为深入探讨磷脂酶C的性质及功能奠定了基础。

关键词：盐藻磷脂酶C 原核表达蛋白纯化蛋白印迹

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油用牡丹籽饼粕中低聚茋类含量测定方法研究

中国粮油学报 2016年第4期31卷 138-141,146页

作者：刘普李小方王永威刘一琼邓瑞雪河南省伏牛山野生药材基源工程技术研究中心河南科技大学化工与制药学院洛阳471023

采用Folin-Ciocalteus比色法测定油用牡丹籽饼粕中低聚芪类化合物的含量。以没食子酸为标准物质，系统研究了Folin-Ciocalteus比色法测定油用牡丹籽饼粕中低聚芪类的方法。得到适宜的显色条件为：显色剂用量为5mL，碳酸钠溶液用量为15m... 详细信息

采用Folin-Ciocalteus比色法测定油用牡丹籽饼粕中低聚芪类化合物的含量。以没食子酸为标准物质，系统研究了Folin-Ciocalteus比色法测定油用牡丹籽饼粕中低聚芪类的方法。得到适宜的显色条件为：显色剂用量为5mL，碳酸钠溶液用量为15mL，反应时间为30min，反应温度为20～25℃。根据低聚芪类化合物与Folin-Ciocaheus反应后的紫外可见光区的特征吸收，由光谱扫描曲线可知最大吸收波长为750nm，通过绘制标准曲线，得到没食子酸的浓度与吸光度之间的线性关系。研究结果表明：在0．5～3．5μg／mL范围内，没食子酸吸光度与其质量呈良好线性关系，R2=0．9996，平均加样回收率99．8％，RSD为0．97％。该方法简便、快速、稳定性好，可以用于低聚芪类化合物的含量测定。

关键词： Folin-Ciocaheus比色法油用牡丹籽饼粕低聚茋类化合物