检索结果-南通市图书馆

Internet search data could Be used as novel indicator for assessing COVID-19 epidemic

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Infectious Disease Modelling 2020年第1期5卷 848-854页

作者： Kang Li Yanling Liang Jianjun Li Meiliang Liu Yi Feng yiming shao Key Laboratory of Molecular Microbiology and Technology Ministry of EducationCollege of Life SciencesNankai UniversityTianjinChina State Key Laboratory for Infectious Disease Prevention and Control National Center for AIDS/STD Control and PreventionChinese Center for Disease Control and PreventionBeijingChina Guangxi Center for Disease Prevention and Control NanningGuangxiChina School of Public Health Guangxi Medical UniversityNanningGuangxiChina

The pandemic of the coronavirus disease(COVID-19)poses a huge challenge all countries,since no one is well prepared for *** be better prepared for future pandemics,we evaluated association between the internet search data with reported COVID-19 cases to verify whether it could become an early indicator for emerging *** the keyword filtering and Index composition,we found that there were close correlations between Composite Index and suspected cases for COVID-19(r?0.921,P<0.05).The Search Index was applied for the Autoregressive Integrated Moving Average with Exogenous Variables(ARIMAX)model to quantify the *** with the model based on surveillance data only,the ARIMAX model had smaller Akaike Information Criterion(AIC?403.51)and the most accurate predictive ***,the Internet search data could serve as a convenient indicator for predicting the epidemic and to monitor its trends.

关键词： COVID-19 Internet data ARIMAX Predict

A new pattern-based method for identifying recent HIV-1 infections from the viral env sequence

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Science China(Life Sciences) 2012年第4期55卷 328-335页

作者： YANG Jing XIA XiaYu HE Xiang YANG SenLin RUAN YuHua ZHAO QuanBi WANG ZhiXin shao yiming PAN XianMing Key Laboratory of Bioinformatics Ministry of Education School of Life Sciences Tsinghua University Beijing 100084 China State Key Laboratory for Infectious Disease Prevention and Control National Center for AIDS/STD Control and Prevention Chinese Center for Disease Control and Prevention Beijing 102206 China

The long asymptomatic stage of HIV infection poses a great challenge in identifying recent HIV infections. This is a bottleneck for monitoring HIV epidemic trends and evaluating the effectiveness of national AIDS control programs. Several serological methods were used to address this issue with some success. Because of high false-positive rates in patients with advanced infection or in ART treatment, UNAIDS still hesitates to recommend their use in routine surveillance. We developed a new pattern-based method for measuring intra-patient viral genetic diversity for determination of recent infections and estimation of population incidence. This method is verified by using several datasets (424 subtype B and 77 CRF07_BC samples) with clearly identified HIV-1 infection times. Pattern-based diversities of recent infections are significantly lower than that of chronic ones. With larger window periods varying from 200 to 350 days, a higher accuracy (90% 95%) not affected by advanced disease nor ART treatment could be obtained. The pattern-based genetic method is supplementary to the existing serology-based assays, both of which could be suitable for use in low and high epidemic regions, respectively.

关键词： determination of HIV recent infections estimation of population incidence viral genetic diversity pattern-based distance

基于HIV-1的pol基因区序列分析我国CRF07_BC毒株的跨地区传播特征

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中国艾滋病性病 2024年第3期30卷 232-239页

作者：王栋冯毅郝静静胡红萍李方园李佳璐廖玲洁阮玉华邵一鸣邢辉中国疾病预防控制中心性病艾滋病预防控制中心北京102206

目的通过构建我国HIV-1的CRF07_BC毒株pol基因区序列的分子网络,分析CRF07_BC毒株在我国的跨地区传播特征,为促进我国HIV精准防控提供数据支持。方法使用FastTree与中国艾滋病病毒基因序列数据平台(https://***/hiv/)的亚型判别工具进... 详细信息

目的通过构建我国HIV-1的CRF07_BC毒株pol基因区序列的分子网络,分析CRF07_BC毒株在我国的跨地区传播特征,为促进我国HIV精准防控提供数据支持。方法使用FastTree与中国艾滋病病毒基因序列数据平台(https://***/hiv/)的亚型判别工具进行亚型判别,筛选出我国CRF07_BC毒株的pol基因序列构建分子网络,使用单因素与多因素Logistic回归分析入网率与跨地区连接影响因素,计算跨地区聚类系数等分子网络分析指标描述不同省份之间的传播连接和强度特征。结果共收集到我国HIV-1毒株pol基因区序列81612条,其中CRF07_BC毒株26181条序列,在0.5%基因距离阈值下构建分子网络,形成2028个分子网络,最大的分子网络含有6262个节点,命名为07-1-1,节点来自全国31个省(自治区、直辖市),以男男性行为传播途径为主(32.0%,2005/6262)。跨地区分析指标显示07-1-1分子网络中68.9%为跨地区连接边,80.6%为跨地区节点;跨地区聚类系数显示分子网络07-1-1的跨地区传播以广东省(66.6%,79114/118819)与北京市(27.9%,33159/118819)为中心,发生在我国华东地区、西南地区、华南地区和华中地区等地区间。结论我国HIV跨地区传播中CRF07_BC毒株起很强的主导作用,广东省与北京市处于跨地区传播的中心位置,有效控制HIV毒株在我国的跨地区传播,将成为我国艾滋病防控高质量发展的重要举措之一。

关键词： 1型艾滋病病毒跨地区传播分子网络聚类系数

IDM editorial statement on the 2019-nCoV

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Infectious Disease Modelling 2020年第1期5卷 233-234页

作者： yiming shao Jianhong Wu State Key Laboratory of Infectious Disease Prevention and Control Chinese Center for Disease Control and Prevention(China CDC)Collaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesBeijingChina Laboratory for Industrial and Applied Mathematics Centre for Disease ModellingDepartment of Mathematics and StatisticsYork UniversityTorontoONM3J 1P3Canada

In December of 2019,27 patients with viral pneumonia including 7 severe cases were reported inWuhan,the capital city of Hubei province and one of the largest cities of *** the morning of January 23,2020,the infected cases have increased to 571 including 17 deaths,and spread to 25 of 31 mainland provinces and municipals and Hong Kong,Macao and Taiwan as well as to 7 other countries,including South Korea,Japan,Thailand,Singapore,Philippines,Mexico and the United States of America.

关键词： 2019-nCoV Wuhan Novel coronavirus World Health Organization(WHO) SARS SARI Severe Acute Respiratory Syndrome

Comparison of different strategies for controlling HIV/AIDS spreading in MSM

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Infectious Disease Modelling 2018年第1期3卷 293-300页

作者： Jie Lou Jinjin Cheng Yan Li Chen Zhang Hui Xing Yuhua Ruan yiming shao Department of Mathematics Shanghai University99 Shangda RoadShanghaiChina Henan Cable TV Network Group Co. LtdZhengzhou BranchZhengzhouChina School of Nursing University of Rochester Medical Center255 Crittenden BlvdRm 2w218RochesterNY14642USA State Key Laboratory of Infectious Disease Prevention and Control(SKLID) Collaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesBeijingChina Guangxi Center for Disease Control and Prevention NanningChina

As proposed in the UNAIDSs 2014 report,to end global AIDS epidemic by 2030,90%of people living with HIV need to be diagnosed,90%of the diagnosed need to receive antiretroviral therapy(ART),and 90%of those on treatment need to achieve viral suppression(90-90-90 strategy).The strategies focus on the *** controls HIV spreading by reducing infectiousness of HIV infected individuals via diagnosis and *** this manuscript,we compared the effects of HIV/AIDS interventions that focus on different individuals in MSM population through a dynamics *** results showed that,the success or not of the”90-90-90”strategies depends on a very important factor:the infectious strength among individuals taking *** highly effective HIV treatment,the”90-90-90”strategies are likely to ***,we call for the combination of both primary prevention among the susceptible with the 90-90-90 strategy among the infected to curb the HIV epidemic in Chinese MSM.

关键词： HIV/AIDS Dynamics model Prevention and treatment strategies

Comparison between sepsis-induced coagulopathy and sepsis-associated coagulopathy criteria in identifying sepsis-associated disseminated intravascular coagulation

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World Journal of Emergency Medicine 2024年第3期15卷 190-196页

作者： Huixin Zhao yiming Dong Sijia Wang Jiayuan Shen Zhenju Song Mingming Xue Mian shao Department of Emergency Medicine Zhongshan HospitalFudan UniversityShanghai 200032China

BACKGROUND:Disseminated intravascular coagulation(DIC)is associated with increased mortality in sepsis *** this study,we aimed to assess the clinical ability of sepsis-induced coagulopathy(SIC)and sepsis-associated coagulopathy(SAC)criteria in identifying overt-DIC and preDIC status in sepsis ***:Data from 419 sepsis patients were retrospectively collected from July 2018 to December *** performances of the SIC and SAC were assessed to identify overt-DIC on days 1,3,7,or *** SIC status or SIC score on day 1,the SAC status or SAC score on day 1,and the sum of the SIC or SAC scores on days 1 and 3 were compared in terms of their ability to identify *** SIC or SAC status on day 1 was evaluated as a pre-DIC indicator for anticoagulant ***:On day 1,the incidences of coagulopathy according to overt-DIC,SIC and SAC criteria were 11.7%,22.0%and 31.5%,*** specificity of SIC for identifying overt-DIC was significantly higher than that of the SAC criteria from day 1 to day 14(P3 had a significantly higher sensitivity(72.00%)and area under the curve(AUC)(0.69)in identifying pre-DIC than did the SIC or SAC status(sensitivity:SIC status 44.00%,SAC status 52.00%;AUC:SIC status 0.62,SAC status 0.61).The sum of the SIC scores on days 1 and 3 had a higher AUC value for identifying the pre-DIC state than that of SAC(0.79 vs.0.69,P<0.001).Favorable effects of anticoagulant therapy were observed in SIC(adjusted hazard ratio[HR]=0.216,95%confidence interval[95%CI]:0.060–0.783,P=0.018)and SAC(adjusted HR=0.146,95%CI:0.041–0.513,P=0.003).CONCLUSION:The SIC and SAC seem to be valuable for predicting *** sum of SIC scores on days 1 and 3 has the potential to help identify pre-DIC.

关键词： Sepsis Disseminated intravascular coagulation Sepsis-induced coagulopathy Sepsis-associated coagulopathy

Impact of HIV Pretreatment Drug Resistance on Virological Failure After One-Year Antiretroviral Therapy--China,2018-2019

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China CDC weekly 2022年第25期4卷 535-540页

作者： Miaomiao Li Chang Song Aobo Dong Jing Hu Ruihua Kang Hui Xing Yuhua Ruan yiming shao Kunxue Hong Lingjie Liao State Key Laboratory of Infectious Disease Prevention and Control National Center for AIDS/STD Control and PreventionChinese Center for Disease Control and PreventionBeijingChina

Summary What is already known about this topic?While antiretroviral therapy(ART)has been rapidly scaled-up among the population living with human immunodeficiency virus or acquired immune deficiency syndrome(HIV/AIDS)... 详细信息

关键词： Therapy Impact patients

2004-2022年我国HIV-1 CRF08_BC亚型感染者抗病毒治疗前耐药分析

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疾病监测 2024年第5期39卷 634-638页

作者：辛沛贤宋畅胡婧刘秀王栋李苗苗李彦潼冯毅阮玉华邢辉邵一鸣廖玲洁传染病溯源预警与智能决策全国重点实验室中国疾病预防控制中心性病艾滋病预防控制中心北京102206

目的了解我国HIV-1 CRF08_BC未抗病毒治疗感染者对蛋白酶类抑制剂(PIs)、核苷类反转录酶抑制剂(NRTIs)、非核苷类反转录酶抑制剂(NNRTIs)的耐药情况。方法收集2004—2022年全国耐药调查以及Los Alamos HIV基因数据库中未治疗感染者的CRF... 详细信息

目的了解我国HIV-1 CRF08_BC未抗病毒治疗感染者对蛋白酶类抑制剂(PIs)、核苷类反转录酶抑制剂(NRTIs)、非核苷类反转录酶抑制剂(NNRTIs)的耐药情况。方法收集2004—2022年全国耐药调查以及Los Alamos HIV基因数据库中未治疗感染者的CRF08_BC亚型序列,利用美国斯坦福大学Los Alamos HIV基因数据库进行耐药判定,采用HIV-TRACE软件进行HIV-1耐药传播网络分析。结果收集HIV-1 CRF08_BC序列4539条,总耐药率为15.27%(693/4539)。PIs、NRTIs和NNRTIs的耐药率分别为1.70%(77/4539)、1.85%(84/4539)、12.91%(586/4539);其中利匹韦林(RPV)的耐药率最高,为11.28%(512/4539)。多重耐药率为0.1%(44/4539)。结论近年来我国CRF08_BC感染者耐药率不断升高,主要由NNRTIs药物耐药率逐年上升引起,其中RPV的耐药率最高,且存在耐药毒株的传播,治疗前耐药对我国抗病毒治疗效果的影响还需进一步监测和分析。

关键词：艾滋病病毒 CRF08_BC亚型抗病毒治疗前传播性耐药耐药突变

具有广谱中和活性的新型冠状病毒受体结合结构域抗体的筛选和鉴定

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中国热带医学 2024年第3期24卷 258-264页

作者：倪婉琦任莉靳昌忠杨馥榕申玉敏王硕胡彩琴郝彦玲刘颖朱彪邵一鸣李丹王铮传染病溯源预警与智能决策全国重点实验室中国疾病预防控制中心性病艾滋病预防控制中心北京102206 浙江大学医学院附属第一医院传染病诊治国家重点实验室浙江杭州310003 南开大学药学院天津300071 天津国际生物医药联合研究院天津300457

目的从2名新型冠状病毒感染康复者体内分离针对新型冠状病毒(severe acute respiratory syndrome coro‐navirus 2,SARS-CoV-2)受体结合结构域(receptor binding domain,RBD)的特异性单克隆B细胞,并筛选出具有广谱中和活性的SARS-CoV-2 RBD中和抗体。方法以生物素化的RBD为分子探针,利用流式细胞仪对康复者外周血单核细胞(peripheral blood mononuclear cells,PBMCs)进行单克隆B细胞分选,将获得的B细胞经裂解并完成逆转录后,采用随机引物对抗体的重链及轻链进行巢式PCR扩增,扩增产物分别克隆至对应的表达载体,随后相应配对的重轻链质粒共转染293F细胞中进行表达,利用Protein A柱纯化获得单克隆抗体。采用原始株(WT)假病毒开展中和实验挑选出半数抑制浓度(IC_(50))<0.1µg/mL的抗体,进一步在原始株(WT)、Beta株(B.1.351)和Delta株(B.1.617.2)活病毒以及目前的流行株XBB、BA.5、BF.7假病毒中进行中和能力及宽度测试。结果从2名康复者体内共获得21个RBD特异性单克隆B细胞,从中分离出13对抗体轻/重链,成功表达出9个抗体,P1-A1、P1-B6、P1-B9对原始株(WT)假病毒IC_(50)值均低于0.1µg/mL,其中P1-B6可以有效中和原始株(WT)、Beta株(B.1.351)和Delta株(B.1.617.2)活病毒,IC_(50)值均达到了0.01µg/mL。P1-B9可以有效中和原始株(WT)、Beta株(B.1.351)、Delta株(B.1.617.2)及Gamma株(P.1)活病毒,IC_(50)值分别为0.42、0.63、0.28、2.50µg/mL。此外,P1-B6对BA.5、BF.7假病毒具有较好的中和效果,IC_(50)值分别为0.06µg/mL和0.09µg/mL。结论SARS-CoV-2原始株感染可以诱导出具有广谱中和活性的抗体,且这些广谱中和抗体的产生不需要过高的体细胞高频突变率,获得的抗体可以作为诊断和预防新冠病毒感染的候选。

关键词：新型冠状病毒中和抗体受体结合结构域