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DNA vaccine prime and replicating vaccinia vaccine boost induce robust humoral and cellular immune responses against MERS-CoV in mice

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Virologica Sinica 2024年第3期39卷 490-500页

作者： Xiuli Shen Shuhui Wang Yanling Hao Yuyu Fu Li Ren Dan Li Wenqi Tang Jing Li Ran Chen Meiling Zhu Shuo Wang Ying Liu yiming shao National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases National Center for AIDS/STD Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Changping Laboratory Yard 28Science Park RoadChangping DistrictBeijing 102206China State Key Laboratory for Diagnosis and Treatment of Infectious Diseases National Clinical Research Center for Infectious DiseasesCollaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesThe First Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhou 310003China

As of December 2022,2603 laboratory-identified Middle East respiratory syndrome coronavirus(MERS-CoV)infections and 935 associated deaths,with a mortality rate of 36%,had been reported to the World Health Organization(WHO).However,there are still no vaccines for MERS-CoV,which makes the prevention and control of MERS-CoV *** this study,we generated two DNA vaccine candidates by integrating MERS-CoV Spike(S)gene into a replicating Vaccinia Tian Tan(VTT)*** to homologous immunization with either vaccine,mice immunized with DNA vaccine prime and VTT vaccine boost exhibited much stronger and durable humoral and cellular immune *** immunized mice produced robust binding antibodies and broad neutralizing antibodies against the EMC2012,England1 and KNIH strains of ***-Boost immunization also induced strong MERS-S specific T cells responses,with high memory and poly-functional(CD107a-IFN-γ-TNF-α)effector CD8t T *** conclusion,the research demonstrated that DNA-Prime/VTT-Boost strategy could elicit robust and balanced humoral and cellular immune responses against *** study not only provides a promising set of MERS-CoV vaccine candidates,but also proposes a heterologous sequential immunization strategy worthy of further development.

关键词： MERS-CoV VTT vaccine DNA vaccine Humoral and cellular immune responses Prime/boost strategy

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城市道路下分心行为对驾驶员驾驶绩效的影响

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中国安全科学学报 2024年第1期34卷 70-76页

作者：罗霜易鑫鑫邵毅明徐进重庆交通大学交通运输学院重庆400074 重庆长安汽车股份有限公司重庆400023

为探究城市道路条件下常见驾驶分心行为对驾驶绩效的影响,以16名青年志愿者为被试,开展实车驾驶试验,测量车辆纵向和横向行驶参数,对比分析正常驾驶和分心驾驶状态下的驾驶绩效,并探讨驾驶经验的影响。结果表明:3种分心行为均使横向加... 详细信息

为探究城市道路条件下常见驾驶分心行为对驾驶绩效的影响,以16名青年志愿者为被试,开展实车驾驶试验,测量车辆纵向和横向行驶参数,对比分析正常驾驶和分心驾驶状态下的驾驶绩效,并探讨驾驶经验的影响。结果表明:3种分心行为均使横向加速度标准差和纵向加速度标准差增加,而使速度均值降低,说明驾驶员通过速度和转向控制补偿分心状态下的驾驶绩效;交谈对速度均值和纵向加速度均值、阅读广告对速度标准差和横向加速度均值具有显著性影响(p<0.05);接听电话时,经验驾驶员速度标准差明显高于新手(p=0.021);接听电话和阅读广告次任务下,经验驾驶员的横向加速度均值均明显较高(p=0.003,p=0.004),说明经验丰富的驾驶员受分心行为的影响程度更低。

关键词：城市道路分心行为驾驶员驾驶绩效实车试验

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纯电动汽车电驱动系统故障诊断研究进展

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汽车工程 2024年第1期46卷 61-74页

作者：张鹏博陈仁祥邵毅明孙世政闫凯波重庆交通大学交通运输学院重庆400074 重庆交通大学交通工程应用机器人重庆市工程实验室重庆400074

为全面梳理纯电动汽车电驱动系统故障诊断的发展现状,明确未来发展趋势,本文首先介绍了纯电动汽车电驱动系统的基本架构、功能及发展历程;然后详细总结了纯电动汽车电驱动系统关键部件的故障类型及原因,分析了纯电动汽车电驱动系统关键... 详细信息

为全面梳理纯电动汽车电驱动系统故障诊断的发展现状,明确未来发展趋势,本文首先介绍了纯电动汽车电驱动系统的基本架构、功能及发展历程;然后详细总结了纯电动汽车电驱动系统关键部件的故障类型及原因,分析了纯电动汽车电驱动系统关键部件故障诊断方法的主要研究现状;接着将诊断方法从专家知识驱动、模型驱动、信号驱动和数据驱动4个方面详细综述了纯电动汽车电驱动系统国内外研究进展和发展动态,并针对不同方法的优缺点进行了对比;最后对纯电动汽车电驱动系统故障诊断所面临的问题及发展方向进行了分析和展望,进一步讨论并指出未来纯电动汽车电驱动系统故障诊断研究可以集中在变工况耦合故障诊断、微小故障诊断和前期故障诊断研究、实时在线故障诊断、基于故障诊断的智能运维、未知故障诊断与系统自愈技术等方面。

关键词：纯电动汽车电驱动系统故障诊断

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MicroRNA 31 inhibits CD8^(+) T-cell function by increasing its sensitivity to type I interferon signaling in chronic viral infection

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Cellular & Molecular Immunology 2018年第5期15卷 533-535页

作者： Mingming Jia yiming shao Kunxue Hong Division of Research on Virology and Immunology State Key Laboratory for Infectious Disease Prevention and ControlNational Center for AIDS/STD Control and PreventionChina CDCCollaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesBeijing 102206China Key Laboratory of Infection and Immunity Institute of BiophysicsChinese Academy of SciencesBeijing 100101China

Chronic viral infections and malignant tumors can cause T cells to become dysfunctional and exhausted through two different associated immune statuses:inflammation and *** immunity is a necessary step for the establishment of the immune response to control infection and ***,once a pathogen cannot be cleared in time or a tumor continues to progress,an immunosuppressive environment involving various immune cells and cytokines may form,thereby promoting the persistence of the pathogen or *** those immune factors,type I interferons play a dual role by driving inflammation and inducing a variety of suppressive factors that limit immune responses in chronic infection and cancer.

关键词： inflammation infection immunity

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入院前使用二甲双胍对糖尿病合并脓毒症患者临床结局的影响

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中国全科医学 2024年第9期27卷 1034-1041页

作者：何宇福佟文英王芳李丽霞何俊冰邵义明广东医科大学附属东莞第一医院重症医学科广东省东莞市523940 广东医科大学研究生院广东省湛江市524000 广东省揭阳市人民医院揭阳市医学科研中心实验室 522000

背景越来越多的证据表明,入院前使用二甲双胍可降低糖尿病合并脓毒症患者的死亡率,然而,二甲双胍对糖尿病合并脓毒症患者临床结局的影响仍存在争议,因此,有必要对现有研究进行系统回顾和荟萃分析,进一步评估二甲双胍是否有利于改善糖尿... 详细信息

背景越来越多的证据表明,入院前使用二甲双胍可降低糖尿病合并脓毒症患者的死亡率,然而,二甲双胍对糖尿病合并脓毒症患者临床结局的影响仍存在争议,因此,有必要对现有研究进行系统回顾和荟萃分析,进一步评估二甲双胍是否有利于改善糖尿病合并脓毒症患者死亡率等临床结局。目的系统评价入院前使用二甲双胍对糖尿病合并脓毒症患者死亡率、初始血浆乳酸水平和器官功能障碍等的影响。方法计算机检索PubMed、Embase、Cochrane Library、Web of Science、Scopus、中国生物医学文献服务系统、万方数据知识服务平台、中国知网以及其他数据库中二甲双胍对糖尿病合并脓毒症患者临床结局影响的相关中、英文文献,试验组(MET组)为入院前使用二甲双胍的糖尿病合并脓毒症的成年患者,对照组(NM组)为入院前未使用二甲双胍的糖尿病合并脓毒症的成年患者,检索时限为数据库建立至2023-03-15。由2名研究人员筛选、提取数据及评价文献质量后,采用RevMan 5.3软件进行Meta分析。结果本研究共纳入12篇文献,共12320例糖尿病合并脓毒症患者,纳入文献的纽卡斯尔-渥太华量表(NOS)评分为7~8分。Meta分析结果显示:MET组患者死亡率(OR=0.61,95%CI=0.46~0.80,P<0.001)、血管升压药的使用率(OR=0.83,95%CI=0.69~0.98,P=0.03)低于NM组;MET组与NM组患者初始血浆乳酸水平(MD=0.31,95%CI=-0.12~0.75,P=0.16)、血清肌酐水平(MD=-0.81,95%CI=-0.48~0.13,P=0.25)、初始血糖水平(MD=32.94,95%CI=-10.12~76.01,P=0.13)、机械通气(OR=0.90,95%CI=0.77~1.06,P=0.23)比较,差异无统计学意义。结论入院前使用二甲双胍可以降低糖尿病合并脓毒症患者的死亡率,并可减少相应患者血管升压药的使用率,为二甲双胍可降低糖尿病合并脓毒症患者死亡率提供了全新的证据。但对于糖尿病合并脓毒症患者能否降低脓毒症严重程度评分以及减少去甲肾上腺素最大剂量仍有待进一步的研究。

关键词：糖尿病脓毒症二甲双胍临床结局死亡率器官功能障碍 Meta分析

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From a Deadly Disease to a Manageable Chronic Disease,HIV/AIDS Remains a Challenge for Mankind

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Infectious Microbes & Diseases 2023年第2期5卷 41-43页

作者： Shimiao shao yiming shao State Key Laboratory for Diagnosis and Treatment of Infectious Diseases National Clinical Research Center for Infectious DiseasesCollaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesThe First Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhouChina State Key Laboratory for Infectious Disease Prevention and Control National Center for AIDS/STD Control and PreventionChinese Center for Disease Control and PreventionBeijingChina.

Acquired immune deficiency syndrome(AIDS)is caused by human immunodeficiency virus(HIV),which was found by French scientists 40 years ago,12 years after AIDS was firstly reported by US scientists.2 TheHIV/AIDS pandemic has spread to every country of theworldwith over 84 million infections and more than 40 million deaths since the start of the epidemic.3 According to Joint United Nations Programme on HIV/AIDS(UNAIDS),there are 38.4 million people living with HIV/AIDS(PLWHA)in 2021.4 In order to realize the Ending AIDS Plan by 2030,the UNAIDS made the intermediate target of less than 500,000 new HIV infections and 500,000 AIDS-related deaths in 2020.5 In the 2021 United Nations(UN)75th General Assembly,the General Secretary reported that the 2020 target of Ending AIDS Plan was not reached,6 with 1.7 million HIV infections and 690,000 AIDS-related deaths in 2019.7 In order to bring the Ending AIDS Plan back on track,UNAIDS setup a higher goal of“95-95-95,”meaning the percentage for all PLWHA who know their HIV status.

关键词： acquired immune deficiency syndrome human immunodeficiency virus combination antiretroviral therapy molecular transmission network

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Depletion and dysfunction of Vy,2Vo2 T cells in HIV disease： mechanisms, impacts and therapeutic implications

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Cellular & Molecular Immunology 2013年第1期10卷 42-49页

作者： Haishan Li Suchita Chaudry Bhawna Poonia yiming shao C David Pauza Institute of Human Virology and Department of Medicine University of Maryland School of Medicine Baltimore MD USA and State Key Laboratory for Infectious Disease Controland Prevention National Center for AIDS/STD Control and Prevention Chinese Center for Disease Control and Prevention Beijing 102206 China

Infection with human immunodeficiency virus （HIV） disrupts the balance among yT cell subsets, with increasing Vo1＋ cells and substantial depletion of circulating Vo2＋ cells. Depletion is an indirect effect of HIV in CD4-negative Vo2 cells, but is specific for phosphoantigen-responsive subpopulations identified by the Vy2-Jy1.2 （also called Vy9-JyP） T cell receptor rearrangement. The extent of cell loss and recovery is related closely to clinical status, with highest levels of functional V cells present in virus controllers （undetectable viremia in the absence of antiretroviral therapy）. We review the mechanisms and clinical consequences for V cell depletion in HIV disease. We address the question of whether HIV-mediated V cell depletion, despite being an indirect effect of infection, is an important part of the immune evasion strategy for this virus. The important roles for V cells, as effectors and immune regulators, identify key mechanisms affected by HIV and show the strong relationships between V62 cell loss and immunodeficiency disease. This field is moving toward immune therapies based on targeting V cells and we now have clear goals and expectations to Ruide interventional clinical trials.

关键词： HIV disease ST cells mechanisms impacts therapeutic implications

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SARS-CoV-2 vaccine research and immunization strategies for improved control of the COVID-19 pandemic

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Frontiers of Medicine 2022年第2期16卷 185-195页

作者： yiming shao Yingqi Wu Yi Feng Wenxin Xu Feng Xiong Xinxin Zhang Chinese Center for Disease Control and Prevention Beijing 102206China Changping Laboratory Beijing 102206China Department of Infectious Diseases Research Laboratory of Clinical VirologyNational Research Center for Translational Medicine at ShanghaiRuijin Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200025China Department of Policy Science Central Committee of the Chinese Peasants and Workers Democratic PartyBeijing 100011China

The record speed at which Chinese scientists identified severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)and shared its genomic sequence with the world,has greatly facilitated the development of coronavirus disease(COVID-19)diagnostics,drugs,and *** is unprecedented in pandemic control history to develop a dozen successful vaccines in the first year and to immunize over half of the global population in the second year,due to the efforts of the scientific community,biopharmaceutical industry,and regulatory agencies *** challenges are both great and multidimensional due to the rapid emergence of virus variants and waning of vaccine *** strategies need to adapt to these challenges to keep population immunity above the herd immunity threshold,by increasing vaccine coverage,especially for older adults and young people,and providing timely booster doses with homologous or heterologous vaccine *** research should be undertaken to develop more effective vaccines against SARS-CoV-2 variants and to understand the best prime-boost vaccine combinations and immunization strategies to provide sufficient and sustainable immune protection against COVID-19.

关键词： COVID-19 pandemics SARS-CoV-2 vaccination

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Low-cost organic photovoltaic materials with great application potentials enabled by developing isomerized non-fused ring acceptors

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Science China Chemistry 2023年第4期66卷 1101-1110页

作者： yiming shao Rui Sun Wei Wang Xinrong Yang Chenkai Sun Yongfang Li Jie Min The Institute for Advanced Studies Wuhan UniversityWuhan 430072China College of Chemistry Green Catalysis CenterZhengzhou UniversityZhengzhou 450001China Beijing National Laboratory for Molecular Sciences CAS Key Laboratory of Organic SolidsInstitute of ChemistryChinese Academy of SciencesBeijing 100190China

Benefitting from low cost and simple synthesis,simple structured non-fused ring acceptors(NFRAs)and polymer donors are crucial for the application of organic solar cells(OSCs).Herein,two isomerized NFRAs,namely 4T-FCl FCl and 4T-2F2Cl,are designed with end-group engineering,which modulates the electrostatic potential distributions and crystallinity of acceptors,and accordingly,the A/A and D/A intermolecular *** OSC based on 4T-2F2Cl with strong D/A interactions shows a record-high efficiency of 16.31%in blending with a low-cost polymer donor PTQ10,which shapes obviously improved bulkheterojunction(BHJ)networks blade-coated by non-halogenated solvent o-xylene,and thus significantly diminishes nonradiative recombination loss.A higher industrial figure of merit(i-FOM)of 0.46 for PTQ10:4T-2F2Cl in comparison with PTQ10:4T-FCl FCl(i-FOM=0.29)is demonstrated owing to its superior device efficiency and operational *** that the i-FOM of PTQ10:4T-2F2Cl is the highest value for OSCs reported so *** work deepens the synergistic effect of the A/A and D/A interactions on achieving desired bulk heterojunction morphology and demonstrates a printable photovoltaic system for low-cost,high-efficiency,stable,and eco-friendly OSCs.

关键词： low-cost photovoltaic materials non-fused ring acceptors intermolecular interactions non-halogenated solvent doctorblade coating

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Premature Stop Codon at Residue 101 within HIV-1 Rev Does Not Influence Viral Replication of Clade BC but Severely Reduces Viral Fitness of Clade B

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Virologica Sinica 2020年第2期35卷 181-190页

作者： Zheng Wang Xiaolin Ji Yanling Hao Kunxue Hong Liying Ma Dan Li yiming shao State Key Laboratory of Infectious Diseases Prevention and Control Collaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesChinese Center for Disease Control and PreventionBeijing 102206China Division of Research of Virology and Immunology National Center for AIDS/STD Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China

HIV-1 Rev is an accessory protein that plays a key role in nuclear exportation,stabilization,and translation of the viral *** of HIV-1 clade BC often shows a truncation of 16 AAs due to a premature stop codon at residue *** stop codon presents the highest frequency in clade BC and the lowest frequency in clade *** order to discover the potential biological effect of this truncation on Rev activity and virus replication of clade BC,we constructed Rev expression vectors of clade BC with or without 16 AAs within C-terminal separately,and replaced the stop codon by Q in a CRF07_BC infectious *** found that 16 AAs truncation had no effect on expression and activity of Rev in clade ***,the mutation from the stop codon to Q had no effect on virus replication of clade ***,to investigate the effect of this truncation on Rev activity and replication capacity of clade B,Rev expression vectors of clade B carrying or lacking 16 AAs in C-terminal were constructed respectively,and residue Q at position 101 within Rev was substituted by the stop codon in a clade B infectious *** was found that 16 AAs truncation significantly down-regulated Rev expression and impaired clade B Rev ***,a Q-to-stop codon substitution within Rev significantly reduced viral replication fitness of clade *** results indicate that the premature stop codon at residue 101 within Rev exerts diverse impact on viral replication among different HIV-1 clades.

关键词： HIV-1 CRF_BC Rev Premature stop codon Replication

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