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Identification and Characterization of Side Population Cells in Human Lung Adenocarcinoma SPC-A1 Cells

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Chinese Journal of Cancer Research 2010年第3期22卷 211-217页

作者： Yan-liang Zhu Long-bang Chen Jing-hua Wang xin-yi Xia Department of Oncology Department of Clinical Laboratory Centre Jinling HospitalMedical College of Nanjing University Nanjing 210002 China

Objective：There has been an increasing interest in recent years in the role of stem *** an extensive understanding of their biology,a major role for stem cells in the malignant process has been proposed and the existence of cancer stem cells（CSCs） has been confirmed in hematopoietic malignancies and solid organ malignancies including brain cancer,breast,prostate,colon,and pancreatic *** cancer is the leading cause of cancer mortality in most large cities of *** is possible that lung cancer contains cancer stem cells responsible for its *** aim of this study is to identify,characterize and enrich the CSC population that drives and maintains lung adenocarcinoma growth and ***：Side population（SP） cell analysis and sorting were applied on human lung adenocarcinoma cell line and an attempt to further enrich them by preliminary serum-free culture before fluorescence activated cell sorting（FACS） was *** cell properties of SP cells were evaluated by their proliferative index,colony-forming efficiency,tumorigenic potential,bi-differentiation capacity and the expression of common stem cell surface ***：Lung cancer cells could grow in a serum-free Medium（SFM） as non-adherent spheres similar to neurospheres or *** proportion of SP cells in cell spheres was significantly higher than that in cells grown as *** cells had a greater proliferative index,a higher colony-forming efficiency and a greater ability to form tumor in *** cells were both CCA positive and SP-C positive while non-SP cells were only SP-C *** cytometric analysis of cell phenotype showed that SP cells expressed CD133 and CD44,the common cell surface markers of cancer stem cells,while non-SP cells only expressed ***：SP cells existed in human lung adenocarcinoma cell lines and they could be further enriched by preliminary serum-free culture before FACS *** cells possessed the properties of cancer st

关键词： Cancer stem cells Side population SPC-A1 cell line

Thoracotomy of an asymptomatic, functional, posterior mediastinal paraganglioma: A case report

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World Journal of Clinical Cases 2019年第12期7卷 1529-1534页

作者： yi-Yu yin Bin Yang Yeni Ait Ahmed hua xin Department of Thoracic Surgery China-Japan Union Hospital of Jilin University Changchun 130000 Jilin Province China National Institutes on Alcohol Abuse and Alcoholism National Institutes of Health Bethesda MD 20892 United States

BACKGROUND Paragangliomas in the mediastinum are rare, accounting for only 1%-2% of all paragangliomas and < 0.3% of all mediastinal tumors. Most paragangliomas are nonfunctional, therefore, asymptomatic functional paragangliomas in the left posterior mediastinum are extremely rare. Perioperative management including preoperative preparation, careful intraoperative procedures, and strict postoperative care is important, and one-stage surgical resection should be performed only after appropriate perioperative measures are undertaken. Because those tumors are rare, it is necessary to report known cases to raise awareness regarding them. CASE SUMMARY We report the case of a 47-year-old male who was admitted to our hospital with the chief complaints of intermittent tearing pain on the left side of the chest and back for more than 10 mo. A chest contrast-enhanced computed tomography scan revealed a round, solid mass in the left posterior mediastinum, with low-density cystic lesions in the middle, and no enlarged lymph nodes in the hilum or mediastinum (Figure 1). After the diagnosis of paraganglioma, the patient was preoperatively given an oral adrenoceptor blocking drug (phenoxybenzamine), and intravenous fluid resuscitation for two weeks, subsequently the patient underwent a one-stage resection of lesions via left thoracotomy. The patient’s blood pressure increased to 220/120 mmHg when the tumor was touched, which could be relieved by symptomatic treatment such as accelerating liquid transfusion or other intervention to lower blood pressure. The patient recovered uneventfully after surgery, with no abnormal blood pressure or recurrence during one year of follow-up *** Surgical resection is the preferred treatment for asymptomatic functional paragangliomas.

关键词： Mediastinal tumor Paraganglioma Pheochromocytoma Hypertension Case report

Total Synthesis of (-)-Tetrahydrolipstatin by the Tandem Mukaiyama-aldol Lactonization

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Chinese Chemical Letters 2005年第11期16卷 1448-1450页

作者： Jian yiN Xiao Bo YANG Zai xin CHEN yi hua ZHANG Center of Drug Discovery China Pharmaceutical University Nanjing 210009 Jiangsu Yabang Pharm. Res. ＆ Dev. Co. Ltd Nanjing 210004

（-）-Tetrahydrolipstatin 1 was synthesized in three steps and 33.8% overall yield from aldehyde 6 by tandem Mukaiyama-aldol lactonization, which offered a concise, efficient and highly diastereoselective route to this antiobesity drug. We also presented a resolution method for preparation of the crucial intermediate, β- （t-butyldimethylsilyloxy）tetradecanal.

关键词： Tetrahydrolipstatin tandem Mukaiyama-aldol lactonization asymmetric synthesis,(R)-β-hydroxy tetradecanal resolution.

Application of Improved Fuzzy Immune PID Controller to Bending Control System

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Journal of Iron and Steel Research International 2011年第3期18卷 28-33页

作者： REN xin-yi DU Feng-shan huaNG hua-gui ZHANG Shang-bin Institute of Mechanical Engineering Yanshan UniversityQinhuangdao 066004HebeiChina

Based on the hydraulic bending control system,the electrohydraulic servo pressure control simulation model is *** into account of the inadequacy of P-type immune feedback controller,an improved fuzzy immune PID controller is put *** on immune feedback principle of biological immune system,the P-type immune feedback controller is connected with conventional PID controller in series and then in parallel with design fuzzy immune PID *** controller parameters can be adjusted on line by the rules of immune feedback controller and fuzzy *** order to gain the optimal parameters of the controller,the parameters of the controller are off-line optimized by the best multiple optimal model PSO *** simulation results indicate that the method has characteristics of small overshoot,short adjusting time and strong anti-interference ability and *** quality of the strip shape can be further improved.

关键词： electrohydraulic bending system immune feedback control fuzzy control PSO algorithm strip shape

Effects of icariin on erectile function and expression of nitric oxide synthase isoforms in castrated rats

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Asian Journal of Andrology 2005年第4期7卷 381-388,页

作者： Wu-Jiang Liu Zhong-Cheng xin hua xin yi-Ming Yuan Long Tian ying-Lu Guo Andrology Center of Peking University First Hospital Beijing 100009 China

Aim： To investigate the effect of icariin on erectile function and the expression of nitric oxide synthase （NOS） isoforms in castrated rats. Methods： Thirty-two adult male Wistar rats were randomly divided into one shamoperated group （A） and three castrated groups （B, C and D）. One week after surgery, rats were treated with normal saline （groups A and B） or oral icariin （1 mg/[kg·day] for group C and 5 mg/[kg·day] for group D） for 4 weeks. One week after treatment, the erectile function of the rats was assessed by measuring intracavernosal pressure （ICP） during electrostimulation of the cavernosal nerve. The serum testosterone （ST） levels, the percent of smooth muscle （PSM） in trabecular tissue, and the expression of mRNA and proteins of neuronal nitric oxide synthase （nNOS）, inducible nitric oxide synthase （iNOS）, endothelial nitric oxide synthase （eNOS） and phosphodiesterase V （PDES） in corpus cavernosum （CC） were also evaluated. Results： ICP, PSM, ST and the expression of nNOS, iNOS, eNOS and PDE5 were significantly decreased in group B compared with those in group A （P 〈 0.01）. However, ICE PSM and the expression of nNOS and iNOS were increased in groups C and D compared with those in group B （P 〈 0.05）. Changes in ST and the expression of eNOS and PDE5 were not significant （P 〉 0.05） in groups C and D compared with those in group B. Conclusion： Oral treatment with icariin （〉 98.6 % purity） for 4 weeks potentially improves erectile function. This effect is correlated with an increase in PSM and the expression of certain NOS in the CC of castrated rats. These results suggest that icariin may have a therapeutic effect on erectile dysfunction.

关键词： icariin corpus cavernosm intracavernosal pressure nitric oxide synthase erectile dysfunction

Laboratory simulation of soil respiration response to environmental conditions in intertidal zones of Jiangsu Province,China

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Water Science and Engineering 2022年第2期15卷 134-140页

作者： Peng Wang Xia Shen Zu-lin hua Xiao-dong Liu yi-xin Ma Key Laboratory of Integrated Regulation and Resource Development on Shallow Lake of Ministry of Education Hohai UniversityNanjing 210098China Yangtze Institute for Conservation and Development Hohai UniversityNanjing 210098China Key Laboratory of Ocean Space Resource Management Technology of Ministry of Natural Resources Hangzhou 310012China State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering Nanjing Hydraulic Research InstituteNanjing 210029China

As major contributor to the blue carbon sink,intertidal zones play a crucial role in the global carbon *** recent years,more attention has been given to the carbon cycle in intertidal ***,due to highly variable and uncertain environmental conditions,it is difficult to clarify the quantitative relationship between soil respiration and environmental factors through in-situ *** this study,the response of soil respiration characteristics to variations in the temperature and water table was investigated using a monitoring apparatus of CO_(2)flux at the soil-air interface in the intertidal *** results showed that soil respiration flux was significantly correlated with temperature,and the correlation best fitted the DoseResp ***,the respiration flux was enhanced with the descent of water table,a relationship could be described by a quadratic *** effect of the water table on soil respiration became more pronounced with the rise of *** results provide significant clarification of the impact of human activities on the carbon cycle in bare intertidal zones and as well as support for numerical simulations of the carbon cycle in bare intertidal zones.

关键词： Intertidal zone Carbon flux Soil respiration Human activities Water table

Correlation between Toll-like Receptor Gene Polymorphisms and Idiopathic Nephrotic Syndrome in Chinese Children

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Current Medical Science 2023年第3期43卷 585-591页

作者： Hui-hui GAO Wei LI xin-yi SHOU Jian-hua MAO Department of Pediatric and Adolescent Children's HospitalZhejiang University School of MedicineNational Clinical Research Center for Child HealthHangzhou 310052China Department of Clinical Laboratory Children's HospitalZhejiang University School of MedicineNational Clinical Research Center for Child HealthHangzhou 310052China Department of Nephrology Children's HospitalZhejiang University School of MedicineNational Clinical Research Center for Child HealthHangzhou 310052China

Objective Idiopathic nephrotic syndrome(INS)is the most common glomerular disease in ***-like receptors(TLRs)have been reported to be associated with response to steroid treatment in children with ***,the correlation between TLR genes and the progression of INS has not yet been *** present study aimed to investigate the association of single-nucleotide polymorphisms(SNPs)in TLR2,TLR4,and TLR9 with susceptibility to INS as well as the clinical phenotyping of steroid responsiveness in Chinese children with *** A total of 183 pediatric inpatients with INS were included and given standard steroid *** on their clinical response to steroids,the patients were classified into three groups:steroid-sensitive nephrotic syndrome(SSNS),steroid-dependent nephrotic syndrome(SDNS),and steroid-resistant nephrotic syndrome(SRNS).A total of 100 healthy children were employed as *** blood genome DNA was extracted from each *** SNPs(rs11536889,rs1927914,rs7869402,rs11536891,rs352140,and rs3804099)in TLR2,TLR4,and TLR9 were selected and detected by multiplex polymerase chain reaction with next-generation sequencing to assess TLR gene *** Among the 183 patients with INS,89(48.6%)had SSNS,73(39.9%)had SDNS,and 21(11.5%)had *** significant difference was found in the genotype distribution between healthy children and patients with ***,the genotype and allele frequencies of TLR4 rs7869402 were significantly different between SRNS and *** with patients with the C allele and CC genotype,patients with the T allele and CT genotype had an increased risk of *** TLR4 rs7869402 affected the steroid response in Chinese children with *** might be a predictor for the early detection of SRNS in this population.

关键词： children idiopathic nephrotic syndrome polymorphisms Toll-like receptor genes steroid resistance

Infinitely Many Solutions to a Class of p-Laplace Equations

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Acta Mathematicae Applicatae Sinica 2019年第4期35卷 770-779页

作者： yi-hua DENG College of Mathematics and Statistics Hengyang Normal University

In this paper, we study a class of p-Laplace equations. Using variational methods, we prove that there are two solutions and one of these solutions is nonnegative. Using recurrence method, we prove that there are infi... 详细信息

关键词： p-Laplace equation variational methods infinitely many solutions nonnegative solution

Membership determination of open cluster NGC 188 based on the DBSCAN clustering algorithm

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Research in Astronomy and Astrophysics 2014年第2期14卷 159-164页

作者： xin-hua Gao School of Information Science and Engineering Changzhou University

High-precision proper motions and radial velocities of 1046 stars are used to determine member stars using three-dimensional （3D） kinematics for open clus- ter NGC 188 based on the density-based spatial clustering of applications with noise （DBSCAN） clustering algorithm. By implementing this algorithm, 472 member stars in the cluster are obtained with 3D kinematics. The color-magnitude diagram （CMD） of the 472 member stars using 3D kinematics shows a well-defined main sequence and a red giant branch, which indicate that the DBSCAN clustering algorithm is very effective for membership determination. The DBSCAN clustering algorithm can ef- fectively select probable member stars in 3D kinematic space without any assumption about the distribution of the cluster or field stars. Analysis results show that the CMD of member stars is significantly clearer than the one based on 2D kinematics, which al- lows us to better constrain the cluster members and estimate their physical parameters. Using the 472 member stars, the average absolute proper motion and radial velocity are determined to be （PMα, PMδ） = （-2.58 ± 0.22, ＋0.17 ± 0.18） mas yr-1 and Vr = -42.35 ± 0.05 km s-1, respectively. Our values are in good agreement with values derived by other authors.

关键词： Galaxy: open clusters and associations: individual (NGC 188) -- stars:kinematics -- Hertzsprung-Russell diagram -- techniques: radial velocities