Identification and Characterization of Side Population Cells in Human Lung Adenocarcinoma SPC-A1 Cells
Identification and Characterization of Side Population Cells in Human Lung Adenocarcinoma SPC-A1 Cells作者机构:Department of Oncology Department of Clinical Laboratory Centre Jinling HospitalMedical College of Nanjing University Nanjing 210002 China
出 版 物:《Chinese Journal of Cancer Research》 (中国癌症研究(英文版))
年 卷 期:2010年第22卷第3期
页 面:211-217页
核心收录:
学科分类:1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题:Cancer stem cells Side population SPC-A1 cell line
摘 要:Objective:There has been an increasing interest in recent years in the role of stem *** an extensive understanding of their biology,a major role for stem cells in the malignant process has been proposed and the existence of cancer stem cells(CSCs) has been confirmed in hematopoietic malignancies and solid organ malignancies including brain cancer,breast,prostate,colon,and pancreatic *** cancer is the leading cause of cancer mortality in most large cities of *** is possible that lung cancer contains cancer stem cells responsible for its *** aim of this study is to identify,characterize and enrich the CSC population that drives and maintains lung adenocarcinoma growth and ***:Side population(SP) cell analysis and sorting were applied on human lung adenocarcinoma cell line and an attempt to further enrich them by preliminary serum-free culture before fluorescence activated cell sorting(FACS) was *** cell properties of SP cells were evaluated by their proliferative index,colony-forming efficiency,tumorigenic potential,bi-differentiation capacity and the expression of common stem cell surface ***:Lung cancer cells could grow in a serum-free Medium(SFM) as non-adherent spheres similar to neurospheres or *** proportion of SP cells in cell spheres was significantly higher than that in cells grown as *** cells had a greater proliferative index,a higher colony-forming efficiency and a greater ability to form tumor in *** cells were both CCA positive and SP-C positive while non-SP cells were only SP-C *** cytometric analysis of cell phenotype showed that SP cells expressed CD133 and CD44,the common cell surface markers of cancer stem cells,while non-SP cells only expressed ***:SP cells existed in human lung adenocarcinoma cell lines and they could be further enriched by preliminary serum-free culture before FACS *** cells possessed the properties of cancer st