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Overexpression pattern,function,and clinical value of proteasome 26S subunit non-ATPase 6 in hepatocellular carcinoma

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World Journal of Clinical Oncology 2025年第2期16卷 76-93页

作者： sheng-sheng Zhou Yu-Ping Ye yi chen Da-Tong Zeng Guang-Cai Zheng Rong-Quan He Bang-Teng Chi Lei Wang Qian Lin Qin-Yan Su yi-Wu Dang Gang chen Jia-Liang Wei Department of Medical Oncology The First Affiliated Hospital of Guangxi Medical UniversityNanning 530021Guangxi Zhuang Autonomous RegionChina Department of Clinical Laboratory The First Affiliated Hospital of Guangxi Medical UniversityNanning 530021Guangxi Zhuang Autonomous RegionChina Department of Pathology The First Affiliated Hospital of Guangxi Medical UniversityNanning 530021Guangxi Zhuang Autonomous RegionChina Department of Pathology Redcross Hospital of Yulin CityYulin 537000Guangxi Zhuang Autonomous RegionChina Department of Surgery Redcross Hospital of Yulin CityYulin 537000Guangxi Zhuang Autonomous RegionChina

BACKGROUND In recent years,many studies have shown that proteasome 26S subunit non-ATPase 6(PSMD6)plays an important role in the occurrence and development of malignant ***,there are no reports on the evaluation of the potential role of PSMD6 in hepatocellular carcinoma(HCC).AIM To comprehensively evaluate the overexpression pattern and clinical significance of PSMD6 in HCC *** This study integrated PSMD6 mRNA expression profiles from 4672 HCC and 3667 non-HCC tissues,along with immunohistochemical scores from 383 HCC and adjacent tissues,to assess PSMD6 overexpression in *** regularly interspaced short palindromic repeats knockout technology evaluated PSMD6’s essential role in HCC cell *** enrichment analysis explored the molecular mechanism of PSMD6 abnormalities in *** sensitivity analysis and molecular docking analysed the effect of abnormal expression of PSMD6 on the drug sensitivity of HCC *** The results of 41 external and two internal datasets showed that PSMD6 mRNA(SMD=0.26,95%CI:0.09-0.42,P<0.05)and protein(SMD=2.85,95%CI:1.19-4.50,P<0.05)were significantly overexpressed in HCC *** integrated analysis results showed that PSMD6 had a significant overexpression pattern in HCC tissues(SMD=0.40,95%CI:0.15-0.66,P<0.05).PSMD6 knockout inhibited HCC cell growth(chronos scores<-1).Functional enrichment implicated ribosome biogenesis and RNA *** enrichment of signalling pathways such as RNA degradation,ribosomes,and chemical carcinogenesis—reactive oxygen *** sensitivity analysis and a molecular docking model showed that high expression of PSMD6 was associated with the tolerance of HCC cells to drugs such as ML323,sepantronium bromide,and *** PSMD6 effectively distinguished HCC tissues(AUC=0.75,95%CI:0.71-0.79).CONCLUSION This study was the first to discover that PSMD6 was overexpressed in HCC ***6 is essential for the growth of HCC cell

关键词： Hepatocellular carcinoma Proteasome 26S subunit non-ATPase 6 Clustered regularly interspaced short palindromic repeats Ribosome biogenesis RNA splicing

Tribological properties in seawater for Ti/TiCN coatings on Ti6Al4V alloy by arc ion plating with different carbon contents

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Rare Metals 2017年第11期36卷 858-864页

作者： Jin-Long Li Gang-yi Cai Hua-sheng Zhong Yong-Xin Wang Jian-Min chen Key Laboratory of Marine Materials and Application Technologies Zhejiang Key Laboratory of Marine Materials and Protection Technologies Ningbo Institute of Materials Technologies and Engineering Chinese Academy of Sciences School of Mechanical and Electrical Engineering Henan University of Technology

TiCN coatings incorporated with Ti buffer layer were deposited on Ti6A14V alloy by arc ion plating. The carbon content in TiCN coatings was varied by controlling flow rates of C2H2 in reactive gas. The Ti/TiCN coatings have a typical structure of columnar crystal with a total thickness of about 2 pro. The elements of Ti, C and N are present as TiN and TiC in TiCN coatings. A little free carbon appears with carbon content increasing in TiCN coatings. For the TiCN coatings, the hardness, friction coefficient and wear rate decrease with the increase in carbon content. In seawater, both friction coefficient and wear rate have an obvious decrease at lower carbon content compared with those in atmosphere. However, the friction coefficient and wear rate only have a slight decrease, while the carbon content reaches or exceeds 10 at% in Ti/TiCN coatings.

关键词： Ti/TiCN coatings Arc ion plating Hardness Seawater Wear

HPPR encodes the hydroxyphenylpyruvate reductase required for the biosynthesis of hydrophilic phenolic acids in Salvia miltiorrhiza

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Chinese Journal of Natural Medicines 2017年第12期15卷 917-927页

作者： WANG Guo-Quan chen Jun-Feng yi Bo TAN He-Xin ZHANG Lei chen Wan-sheng School of Biomedical Sciences Huaqiao University Department of Pharmaceutical Botany School of Pharmacy Second Military Medical University Department of Pharmacy Changzheng Hospital Second Military Medical University

Salvia miltiorrhiza is a medicinal plant widely used in the treatment of cardiovascular and cerebrovascular diseases. Hydrophilic phenolic acids, including rosmarinic acid(RA) and lithospermic acid B(LAB), are its primary medicinal ingredients. However, the biosynthetic pathway of RA and LAB in S. miltiorrhiza is still poorly understood. In the present study, we accomplished the isolation and characterization of a novel S. miltiorrhiza Hydroxyphenylpyruvate reductase(HPPR) gene, Sm HPPR, which plays an important role in the biosynthesis of RA. Sm HPPR contained a putative catalytic domain and a NAD(P)H-binding motif. The recombinant Sm HPPR enzyme exhibited high HPPR activity, converting 4-hydroxyphenylpyruvic acid(p HPP) to 4-hydroxyphenyllactic acid(p HPL), and exhibited the highest affinity for substrate 4-hydroxyphenylpyruvate. Sm HPPR expression could be induced by various treatments, including SA, GA_3, Me JA and Ag^+, and the changes in Sm HPPR activity were correlated well with hydrophilic phenolic acid accumulation. Sm HPPR was localized in cytoplasm, most likely close to the cytosolic NADPH-dependent hydroxypyruvate reductase active in photorespiration. In addition, the transgenic S. miltiorrhiza hairy roots overexpressing Sm HPPR exhibited up to 10-fold increases in the products of hydrophilic phenolic acid pathway. In conclusion, our findings provide a new insight into the synthesis of active pharmaceutical compounds at molecular level.

关键词： Salvia miltiorrhiza Hydrophilic phenolic acids Rosmarinic acid Llithospermic acid B Biosynthesis pathway

LC-MS/MS analysis and pharmacokinetic study on five bioactive constituents of Tanreqing injection in rats

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Chinese Journal of Natural Medicines 2016年第10期14卷 769-775页

作者： ZHANG Feng SUN Liang GAO Shou-Hong chen Wan-sheng CHAI yi-Feng Department of Pharmacy Changzheng HospitalSecond Military Medical University Department of Pharmaceutical Analysis School of Pharmacy Second Military Medical University Department of Pharmacy The 98th Hospital of PLA

Tanreqing injection(TRQ), a well-known traditional Chinese medicine formula, is commonly used to treat respiratory diseases. In the present study, a rapid, selective, and sensitive liquid chromatography-tandem mass spectrometry(LC-MS/MS) method was developed and validated to simultaneously determinate the plasma contents of 5 major constituents of TRQ, including chlorogenic acid(CHA), caffeic acid(CFA), baicalin(BA), ursodeoxycholic acid(UDCA) and chenodeoxycholic acid(CDCA) in rats after intravenous administration of TRQ. Chromatographic separation was performed on an Agilent Zorbax SB-C_(18) column(3.5 μm, 100 mm × 2.1 mm), with acetonitrile and 0.1% aqueous formic acid as mobile phase at a flow rate of 0.3 m L·min^(^(-1)). The calibration curves were linear over the ranges of 27.0–13 333.0 ng·m L^(-1) for CFA, 30.0–14 933.0 ng·m L^(-1) for CHA, 50.0–50 333.0 ng·m L^(-1) for BA, 550.0–55 000.0 ng·m L^(-1) for UDCA, and 480.0–48 000.0 ng·m L^(-1) for CDCA, respectively. Intra- and inter-day precisions(relative standard deviations, RSDs) were from 3.11% to 14.08%. The extraction recoveries were greater than 71% and accuracy(relative recovery) was from 89% to 137% for all analytes, except endogenous bile acids. This validated method was successfully applied to the first pharmacokinetic study of CFA, CHA, BA, UDCA and CDCA in rat plasma after intravenous administration of TRQ.

关键词： Tanreqing Injection Flavones Phenolic acids Bile acids Pharmacokinetics

EXTRAOCULAR CELLULAR PHOTOTRANSDUCTION

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Journal of Innovative Optical Health Sciences 2009年第1期2卷 93-100页

作者： LING ZHU TIMON chenG-yi LIU MINWU JIAN-QIN YUAN TONG-sheng chen Laboratory of Laser Sports Medicine South China Normal University Guangzhou GD 510006China MOE Key Laboratory of Laser Life Science South China Normal University Guangzhou GD 510631China

Photobiomodulation(PBM)is a modulation of monochromatic light or laser irradiation(LI)on *** is reviewed from the viewpoint of extraocular phototransduction in this *** was found that LI can induce extraocular phototransduction,and there may be an exact correspondence relationship of LI at different wavelengths and in different dose zones,and cellular signal transduction *** signal transduction pathways can be classified into two types so that the Gs protein-mediated pathways belong to pathway 1,and the other pathways such as protein kinase Cs-mediated pathways and mitogen-activated protein kinase-mediated pathways belong to pathway *** all the present pathways found to mediate PBM belong to pathway 2,but there should be a pathway 1-mediated *** previous studies were rather preliminary,and therefore further work should be done.

关键词： Phototransduction photobiomodulation homeostasis

Multi-channel terahertz focused beam generator based on shared-aperture metasurface

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Chinese Physics B 2023年第12期32卷 341-347页

作者：李九生陈翊 Centre for THz Research China Jiliang UniversityHangzhou 310018China

Most of existing metasurfaces usually have limited channel behavior,which seriouslyhinders their development and *** this paper,we propose a multi-channel terahertz focused beam generator based on shared-aperture metasurface,and the generator consists of a top square metal strip,a middle layer of silica and a metal bottom *** changing the position and size of the shared-aperture array,the designed metasurface can generate any number of multi-channel focusing beams at different predicted *** addition,the energy intensity of focusing beams can be *** full-wave simulation results show that the metasurface achieves four-channel vortex focused beam generation with different topological charges,and five-,six-,eight-channel focused beam generation with different energy intensities at a frequency of 1 THz,which are in good agreement with the theoretically calculated *** work can provide a new idea for designing the terahertz multichannel devices.

关键词： terahertz wave multi-channel shared-aperture focused beam

Organoids revealed:morphological analysis of the profound next generation in-vitro model with artificial intelligence

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Bio-Design and Manufacturing 2023年第3期6卷 319-339页

作者： Xuan Du Zaozao chen Qiwei Li sheng Yang Lincao Jiang yi Yang Yanhui Li Zhongze Gu State Key Laboratory of Bioelectronics School of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096China State Key Laboratory for Novel Software Technology Nanjing UniversityNanjing 210008China Key Laboratory of Environmental Medicine Engineering Ministry of EducationSchool of Public HealthSoutheast UniversityNanjing 210009China

In modern terminology,“organoids”refer to cells that grow in a specific three-dimensional(3D)environment in vitro,sharing similar structures with their source organs or *** themorphology or growth characteristics of organoids through a microscope is a commonly used method of organoid ***,it is difficult,time-consuming,and inaccurate to screen and analyze organoids only manually,a problem which cannot be easily solved with traditional *** intelligence(AI)technology has proven to be effective in many biological and medical research fields,especially in the analysis of single-cell or hematoxylin/eosin stained tissue *** used to analyze organoids,AI should also provide more efficient,quantitative,accurate,and fast *** this review,we will first briefly outline the application areas of organoids and then discuss the shortcomings of traditional organoid measurement and analysis ***,we will summarize the development from machine learning to deep learning and the advantages of the latter,and then describe how to utilize a convolutional neural network to solve the challenges in organoid observation and ***,we will discuss the limitations of current AI used in organoid research,as well as opportunities and future research directions.

关键词： Artificial intelligence Organoids Morphology Growth characteristics Deep learning Convolutional neural network

Fractal model of spontaneous imbibition in low-permeability reservoirs coupled with heterogeneity of pore seepage channels and threshold pressure

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Petroleum Science 2024年第2期21卷 1002-1017页

作者： Ming-sheng Zuo Hao chen Xi-Liang Liu Hai-Peng Liu yi Wu Xin-Yu Qi National Key Laboratory of Petroleum Resources and Engineering China University of Petroleum(Beijing)Beijing102249China College of Safety and Ocean Engineering China University of Petroleum(Beijing)Beijing102249China

Spontaneous imbibition(SI)is an important mechanism for enhancing oil recovery in low-permeability *** to the strong heterogeneity,and the non-Darcy flow,the construction of SI model for lowpermeability reservoirs is extremely ***,traditional SI models based on single or averaged capillary tortuosity ignore the influence of heterogeneity of pore seepage channels and the threshold pressure(TP)on ***,in this work,based on capillary model and fractal theory,a mathematical model of characterizing SI considering heterogeneity of pore seepage channels is *** this basis,the threshold pressure was introduced to determine the pore radius at which the wetted phase can displace *** proposed new SI model was verified by imbibition experimental *** study shows that for weakly heterogeneous cores with permeability of 0-1 m D,the traditional SI model can characterize the imbibition process relatively accurately,and the new imbibition model can increase the coefficient of determination by 1.05 ***,traditional model has serious deviations in predicting the imbibition recovery for cores with permeability of 10-50 m *** new SI model coupling with heterogeneity of pore seepage channels and threshold pressure effectively solves this problem,and the determination coefficient is increased from 0.344 to 0.922,which is increased by2.68 *** low-permeability reservoirs,the production of the oil in transitional pores(0.01-0.1μm)and mesopores(0.1-1μm)significantly affects the imbibition recovery,as the research shows that when the heterogeneity of pore seepage channels is ignored,the oil recovery in transitional pores and mesopores decreases by 7.54%and 4.26%,*** analysis shows that increasing interfacial tension,decreasing contact angle,oil-water viscosity ratio and threshold pressure will increase imbibition *** addition,there are critical values for the influence of these factors o

关键词： Spontaneous imbibition Low-permeability reservoir Fractal model Threshold pressure Capillary tube

Roles of mechanosensitive channel Piezo 1/2 proteins in skeleton and other tissues

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Bone Research 2021年第4期9卷 456-472页

作者： Lei Qin Tailin He sheng chen Dazhi Yang Weihong yi Huiling Cao Guozhi Xiao Department of Orthopedics Huazhong University of Science and Technology Union Shenzhen HospitalShenzhenGuangdongChina Department of Biochemistry School of MedicineGuangdong Provincial Key Laboratory of Cell Microenvironment and Disease ResearchShenzhen Key Laboratory of Cell MicroenvironmentSouthern University of Science and TechnologyShenzhenGuangdongChina Department of Orthopedics Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubeiChina

Mechanotransduction is a fundamental ability that allows living organisms to receive and respond to physical signals from both the external and internal *** mechanotransduction process requires a range of special proteins termed mechanotransducers to convert mechanical forces into biochemical signals in *** Piezo proteins are mechanically activated nonselective cation channels and the largest plasma membrane ion channels reported thus *** regulation of two family members,Piezol and Piezo2#has been reported to have essential functions in mechanosensation and transduction in different organs and ***,the predominant contributions of the Piezo family were reported to occur in the skeletal system,especially in bone development and mechano-stimulated bone *** we review current studies focused on the tissue-specific functions of Piezol and Piezo2 in various backgrounds with special highlights on their importance in regulating skeletal cell *** this review,we emphasize the diverse functions of Piezol and Piezo2 and related signaling pathways in osteoblast lineage cells and *** also summarize our current understanding of Piezo channel structures and the key findings about PIEZO gene mutations in human diseases.

关键词： mec sensation organs