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Exploring the intrinsic course of excitability in neurons

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Exploring the intrinsic course of excitability in neurons

出芽酵母Saccharomyces cerevisiae转录抑制因子Rdr1负调控细胞胁迫应答

中国神经科学学会第四次会员代表大会暨第七届全国学术会议

作者： San-Jue HU,Jing YANG,yi-hui liu Institute of Neuroscience,the Fourth Military Medical University,Xi''an 710032

＜正＞For a long time,the ability of neurons to respond to an electrical shock with an action potential is a property referred to as an *** threshold voltage or firing number is as an index measuring degrees of excitability. Actually,in view of neural information encoding the course of excitation is transferring stimulus into firing series which carry the stimulus information with certain firing patterns and it is inadequate to describe this information coding procedure by threshold or firing *** intrinsic course of excitability is still poorly *** tonic,irregular and bursting firing,we have observed various kinds of firing pattern in the dorsal root ganglion neurons,such as periodadding bifurcation,integral multiple phenomenon,slow wave oscillation and chaotic firing patterns,*** variability of firing pattern is confirmed to reflect some nonlinear features of neuronal *** also observed that the generation of firing patterns depends not only on the electrophysiological properties of neurons but also on their dynamical properties. Neurons possess similar electrophysiological features may respond to the same stimulation with very different firing patterns depending on their dynamical states,such as periodic or non-periodic（chaotic） states,or the distance to bifurcation point,*** novel phenomena have been observed respectively,and our terms for them are:"non-periodic sensitivity","critical sensitivity" and "trigger oscillation".Recently three classes of excitability and their transformation in the mesencephalic V neurons were discovered in our *** different types of bifurcation depending on dynamic change of the relative proportion of g4-AP and gNaP is documented as a crucial mechanism underlying the classification and its *** study on the relationship between excitability classes,bifurcations,and interaction of ionic currents will contribute to reveal the intrinsic course of excitability.

关键词： neurons excitability firing patterns bifurcation

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生物化学与生物物理进展 2009年第12期36卷 1544-1552页

作者：苗敏曹红平钟彦刘军王一慧刘昕张年辉刘科四川大学生命科学学院生物资源与生态环境教育部重点实验室成都610064

Rdr1是出芽酵母Saccharomyces cerevisiae的一个转录抑制因子,参与控制细胞的多重药物耐受性,并可能与细胞胁迫应答相关.利用PCR方法扩增RDR1基因片段,将其克隆至高拷贝表达载体pYES2/NTA上并诱导Rdr1蛋白在酵母细胞中过表达.为了揭示... 详细信息

Rdr1是出芽酵母Saccharomyces cerevisiae的一个转录抑制因子,参与控制细胞的多重药物耐受性,并可能与细胞胁迫应答相关.利用PCR方法扩增RDR1基因片段,将其克隆至高拷贝表达载体pYES2/NTA上并诱导Rdr1蛋白在酵母细胞中过表达.为了揭示转录抑制因子Rdr1在胁迫应答中的作用,比较了RDR1过表达细胞、RDR1缺失突变体细胞和野生型细胞在过氧化氢处理、热胁迫和高盐处理条件下的生长状态,结果显示,RDR1过表达导致细胞对上述3种胁迫作用更敏感,而RDR1缺失则使细胞对这些胁迫作用的耐受性不受影响或有一定增强.为了揭示上述不同细胞在胁迫条件下生长状态的差异与细胞内抗氧化酶活性之间的关系,测定并比较了RDR1过表达细胞、RDR1缺失突变体细胞和野生型细胞中超氧化物岐化酶(superoxide dismutase SOD)、过氧化氢酶、葡萄糖-6-磷酸脱氢酶(glucose-6-phosphate dehydrogenase G6PDH)、谷胱甘肽还原酶(glutathione reductase GR)的活性.结果表明,RDR1缺失突变体细胞具高活性的SOD、过氧化氢酶、G6PDH和GR,而Rdr1过表达细胞中SOD、过氧化氢酶、G6PDH和GR的活性较低.RDR1对SOD和过氧化氢酶活性的影响要大于G6PDH和GR.细胞抗氧化酶活性的变化初步揭示,RDR1过表达细胞对胁迫的敏感和RDR1缺失突变体细胞对胁迫耐受性增加的原因.为转录抑制因子Rdr1在胁迫应答中的负调控作用及其机理提供了初步的遗传学和生物化学证据.

关键词： Rdr1 转录因子胁迫应答抗氧化酶

蛋白激酶Cα对人正常肝和肝癌细胞中Ha-ras基因表达的影响

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生物化学与生物物理进展 2002年第5期29卷 781-785页

作者：冯怡柳惠图高萍北京师范大学生命科学学院细胞增殖及调控生物学教育部重点实验室北京100875

运用基因转染技术 ,将蛋白激酶C(PKCα)cDNA正向插入的真核表达重组质粒pXJ4 1 PKCα ,导入人正常肝细胞 (L 0 2 ) ,经蛋白质免疫印迹等检验 ,表明成功构建了稳定过表达PKCα的人正常肝细胞模型 (LT3) ,用LT3和表达反义PKCα的人肝癌细... 详细信息

运用基因转染技术 ,将蛋白激酶C(PKCα)cDNA正向插入的真核表达重组质粒pXJ4 1 PKCα ,导入人正常肝细胞 (L 0 2 ) ,经蛋白质免疫印迹等检验 ,表明成功构建了稳定过表达PKCα的人正常肝细胞模型 (LT3) ,用LT3和表达反义PKCα的人肝癌细胞HT6为细胞模型 ,通过RT PCR ,蛋白质印迹 (Westernblot)等分析和进一步运用自行构建的真核表达质粒prasGL3,进行荧光素酶活性检测表明 :过表达PKCα可以促进L 0 2细胞的增殖速率 ,并引起Ha ras基因转录水平上升和Ha ras启动子活性升高 ;反之 ,表达反义PKCα的BEL 74 0 2细胞增殖被抑制 ,Ha ras基因转录水平下降 ,Ha ras启动子活性降低 .通过实验我们首次观察到 ,PKCα亚类对Ha ras癌基因表达的影响与其对Ha ras启动子活性的作用有关 ,PKCα可能参与了对Ha

关键词：蛋白激酶Cα 人正常肝肝癌细胞 Ha-ras 基因表达

Recent progress in nuclear astrophysics research and its astrophysical implications at the China Institute of Atomic Energy

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Nuclear Science and Techniques 2024年第12期35卷 376-408页

作者： Wei-Ping liu Bing Guo Zhu An Bao-Qun Cui Xiao Fang Chang-Bo Fu Bin-Shui Gao Jian-Jun He Yu-Chen Jiang Chong Lv Er-Tao Li Ge-Xing Li Yun-Ju Li Zhi-Hong Li Gang Lian Wei-Ping Lin yi-hui liu Wei Nan Wei-Ke Nan Yang-Ping Shen Na Song Jun Su Liang-Ting Sun Xiao-Dong Tang Luo-Huan Wang Shuo Wang You-Bao Wang Di Wu Xiao-Feng Xi Sheng-Quan Yan Li-Yong Zhang China Institute of Atomic Energy Beijing 102413China Department of Physics Southern University of Science and TechnologyShenzhen 518055China Jinping Deep Underground Frontier Science and Dark Matter Key Laboratory of Sichuan Province Liangshan 615000China School of Physics Xi’an Jiaotong UniversityXi’an 710049China Key Laboratory of Radiation Physics and Technology of the Ministry of Education Institute of Nuclear Science and TechnologySichuan UniversityChengdu 610064China Sino-French Institute of Nuclear Engineering and Technology Sun Yat-sen UniversityZhuhai 519082China Key Laboratory of Nuclear Physics and Ion-beam Application(MoE) Institute of Modern PhysicsFudan UniversityShanghai 200433China Joint Department for Nuclear Physics Institute of Modern PhysicsChinese Academy of Sciences and Lanzhou UniversityLanzhou 730000China CAS Key Laboratory of High Precision Nuclear Spectroscopy Institute of Modern PhysicsChinese Academy of SciencesLanzhou 73000China School of Nuclear Science and Technology University of Chinese Academy of SciencesBeijing 100049China Key Laboratory of Beam Technology of Ministry of Education College of Nuclear Science and TechnologyBeijing Normal UniversityBeijing 100875China College of Physics and Optoelectronic Engineering Shenzhen UniversityShenzhenChina Key Laboratory of Beam Technology and Material Modification of Ministry of Education College of Nuclear Science and TechnologyBeijing Normal UniversityBeijing 100875China Institute of Modern Physics Chinese Academy of SciencesLanzhou 730000China School of Mathematics and Physics Handan UniversityHandan 056005China Shandong Provincial Key Laboratory of Optical Astronomy and Solar-Terrestrial Environment Institute of Space SciencesShandong UniversityWeihai 264209China State Key Laboratory of Nuclear Physics and Technology School of PhysicsCAPTPeking UniversityBeijing 100871China

Nuclear astrophysics is a rapidly developing interdisciplinary feld of research that has received extensive attention from the scientifc community since the midtwentieth ***,it uses the laws of extremely small atomic nuclei to explain the evolution of the *** to the complexity of nucleosynthesis processes and our limited understanding of nuclear physics in astrophysical environments,several critical astrophysical problems remain *** achieve a better understanding of astrophysics,it is necessary to measure the cross sections of key nuclear reactions with the precision required by astrophysical *** measurement of nuclear reaction cross sections is an important method of investigating how nuclear reactions infuence stellar *** the challenges involved in measuring the extremely low crosssections of nuclear reactions in the Gamow peak and preparing radioactive targets,indirect methods,such as the transfer reaction,coulomb dissociation,and surrogate ratio methods,have been developed over the past several *** are powerful tools in the investigation of,for example,neutron-capture(n,r)reactions with short-lived radioactive ***,direct measurement is still preferable,such as in the case of reactions involving light and stable *** an essential part of stellar evolution,these low-energy stable nuclear reactions have been of particular interest in recent *** overcome the diffculties in measurements near or deeply within the Gamow window,the combination of an underground laboratory and high-exposure accelerator/detector complex is currently the optimal ***,underground experiments have emerged as a new and promising direction of *** addition,to better simulate the stellar environment in the laboratory,research on nuclear physics under laser-driven plasma conditions has gradually become a frontier *** recent years,the CIAE team conducted a series of distinctive nuclea

关键词： Nuclear astrophysics Indirect method Underground laboratory Direct measurement Lowenergy nuclear reaction

PI3K/AKT signaling and neuroprotection in ischemic stroke:molecular mechanisms and therapeutic perspectives

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Neural Regeneration Research 2025年第10期20卷 2758-2775页

作者： Tianlong liu Xiaolin Li Xiaowei Zhou Wei Chen Aidong Wen Minna liu yi Ding Department of Pharmacy Xijing HospitalFourth Military Medical UniversityXi’anShaanxi ProvinceChina Department of Pharmacy The 940 Hospital of Joint Logistics Support Forces of Chinese PLALanzhouGansu ProvinceChina Department of Pharmacy The 944 Hospital of Joint Logistics Support Forces of Chinese PLAJiuquanGansu ProvinceChina Department of Nephrology The 940 Hospital of Joint Logistics Support Forces of Chinese PLALanzhouGansu ProvinceChina

It has been reported that the PI3K/AKT signaling pathway plays a key role in the pathogenesis of ischemic *** a result,the development of drugs targeting the PI3K/AKT signaling pathway has attracted increasing attention from *** article reviews the pathological mechanisms and advancements in research related to the signaling pathways in ischemic stroke,with a focus on the PI3K/AKT signaling *** key findings include the following:(1)The complex pathological mechanisms of ischemic stroke can be categorized into five major types:excitatory amino acid toxicity,Ca^(2+)overload,inflammatory response,oxidative stress,and apoptosis.(2)The PI3K/AKT-mediated signaling pathway is closely associated with the occurrence and progression of ischemic stroke,which primarily involves the NF-κB,NRF2,BCL-2,mTOR,and endothelial NOS signaling pathways.(3)Natural products,including flavonoids,quinones,alkaloids,phenylpropanoids,phenols,terpenoids,and iridoids,show great potential as candidate substances for the development of innovative anti-stroke medications.(4)Recently,novel therapeutic techniques,such as electroacupuncture and mesenchymal stem cell therapy,have demonstrated the potential to improve stroke outcomes by activating the PI3K/AKT signaling pathway,providing new possibilities for the treatment and rehabilitation of patients with ischemic *** investigations should focus on the direct regulatory mechanisms of drugs targeting the PI3K/AKT signaling pathway and their clinical translation to develop innovative treatment strategies for ischemic stroke.

关键词： apoptosis autophagy inflammation ischemic stroke neuroprotection oxidative stress pathogenesis phosphatidylinositol 3-kinase protein kinase B therapeutics

Unraveling brain aging through the lens of oral microbiota

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Neural Regeneration Research 2025年第7期20卷 1930-1943页

作者： Qinchao Hu Si Wang Weiqi Zhang Jing Qu Guang-hui liu State Key Laboratory of Membrane Biology Institute of ZoologyChinese Academy of SciencesBeijingChina Hospital of Stomatology Guanghua School of StomatologyGuangdong Provincial Key Laboratory of StomatologySun Yat-sen UniversityGuangzhouGuangdong ProvinceChina Advanced Innovation Center for Human Brain Protection and National Clinical Research Center for Geriatric Disorders Xuanwu HospitalCapital Medical UniversityBeijingChina Aging Translational Medicine Center International Center for Aging and CancerBeijing Municipal Geriatric Medical Research CenterXuanwu HospitalCapital Medical UniversityBeijingChina CAS Key Laboratory of Genomic and Precision Medicine Beijing Institute of GenomicsChinese Academy of Sciences and China National Center for BioinformationBeijingChina University of Chinese Academy of Sciences BeijingChina Institute for Stem Cell and Regeneration Chinese Academy of SciencesBeijingChina Aging Biomarker Consortium BeijingChina State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijingChina Beijing Institute for Stem Cell and Regenerative Medicine BeijingChina

The oral cavity is a complex physiological community encompassing a wide range of *** of oral microbiota can lead to various oral infectious diseases,such as periodontitis and tooth decay,and even affect systemic health,including brain aging and neurodegenerative *** studies have highlighted how oral microbes might be involved in brain aging and neurodegeneration,indicating potential avenues for intervention *** this review,we summarize clinical evidence demonstrating a link between oral microbes/oral infectious diseases and brain aging/neurodegenerative diseases,and dissect potential mechanisms by which oral microbes contribute to brain aging and *** also highlight advances in therapeutic development grounded in the realm of oral microbes,with the goal of advancing brain health and promoting healthy aging.

关键词： Alzheimer's disease brain aging multiple sclerosis neurodegeneration neurodegenerative diseases oral microbiota Parkinson's disease periodontitis bacteria Porphyromonas gingivalis

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掺金单晶硅特性的研究

物理学报 1992年第3期41卷 491-499页

作者：陈敏锐沈华刘士毅厦门大学物理系厦门361005

本文研究单晶硅掺金前后的表面光伏和红外吸收谱,证实在相同表面状况下,红外吸收谱的基线与少子扩散长度的对应关系;由半导体统计,推导出简并因子不等于1时扩金硅的统计公式,以及金受主简并因子gAu,≠1,金施主简并因子gAu,d≠1硅双重能... 详细信息

本文研究单晶硅掺金前后的表面光伏和红外吸收谱,证实在相同表面状况下,红外吸收谱的基线与少子扩散长度的对应关系;由半导体统计,推导出简并因子不等于1时扩金硅的统计公式,以及金受主简并因子gAu,≠1,金施主简并因子gAu,d≠1硅双重能级复合理论公式,由此计算的少子寿命值与测量值之比在1.64—0.745之间。

关键词：单晶硅掺杂表面光伏红外吸收谱

正清风痛宁缓释片治疗强直性脊柱炎随机对照临床研究Meta分析

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中国中药杂志 2018年第16期43卷 3382-3390页

作者：王媛刘春香张俊华秦艺珊王辉刘源天津中医药大学天津300193 天津中医药大学循证医学中心天津300193

该文通过系统检索相关电子数据库，中文检索中国知识资源总库，万方学术期刊数据库，维普数据库；英文检索PubMed，EMbase，Cochrane Library。检索时间均从建库至2017年9月。文献筛选和质量评价由2名评价员独立完成，采用cochrance系... 详细信息

该文通过系统检索相关电子数据库，中文检索中国知识资源总库，万方学术期刊数据库，维普数据库；英文检索PubMed，EMbase，Cochrane Library。检索时间均从建库至2017年9月。文献筛选和质量评价由2名评价员独立完成，采用cochrance系统评价方法评价纳入研究的方法学质量，使用RevMan5．3软件对所纳入的研究正清风痛宁缓释片治疗强直性脊柱炎的临床疔效和安全性进行Meta分析。最终纳入7篇随机对照试验，共570例强直性脊柱炎患者，正清风痛宁治疗组294例，西药对照组276例。Meta分析结果显示：正清风痛宁缓释片单用治疗组总有效率优于柳氮磺胺吡啶对照组，相对于柳氮磺胺吡啶对照组能明显缩短晨僵时间、指地距，而枕臂试验数值、扩胸度、Schober试验数值、医生评分、患者评分SR，CRP指标水平无明显统计学意义；正清风痛宁缓释片联合西药治疗组总有效率优于西药对照组，相对于西药对照组能明显缩短晨僵时间，明显增大Schober试验数值，而枕臂试验数值，指地距，扩胸度，医生评分，患者评分，ESR，CRP指标，2组无明显统计学意义。正清风痛宁临床应用不良反应较少，单用不良反应以皮肤瘙痒或皮疹多见。该研究结果表明正清风痛宁缓释片可有效治疗强直性脊柱炎，安全性较高，但尚需高质量的临床研究以进一步证明其临床疗效和安全性。

关键词：正清风痛宁缓释片强直性脊柱炎随机对照系统评价 Meta分析

Real-world evidence on the efficacy and safety of vonoprazanamoxicillin dual therapy for Helicobacter pylori treatment in elderly patients

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World Journal of Gastroenterology 2025年第1期31卷 68-78页

作者： Wen Gao Jing-Wen Li hui Ye Xue-Zhi Zhang Jian-Xiang liu Hong Cheng Department of Gastroenterology Peking University First HospitalBeijing 100034China School of Medicine Tsinghua MedicineTsinghua UniversityBeijing 100034China Department of Traditional Chinese Medicine and Integrative Medicine Peking University First HospitalBeijing 100034China

BACKGROUND A dual therapy regimen containing amoxicillin is a common treatment option for the eradication of Helicobacter pylori(***).While substantial research supports the efficacy and safety of vonoprazan and amoxicillin(VA)dual therapy in the general population,there is still a lack of studies specifically focusing on its safety in elderly *** To evaluate efficacy and safety of VA dual therapy as first-line or rescue treatment for *** in elderly *** As a real-world retrospective study,data were collected from elderly patients aged 60 years and above who accepted VA dual therapy(vonoprazan 20 mg twice daily+amoxicillin 1000 mg thrice daily for 14 days)for *** eradication in the Department of Gastroenterology at Peking University First Hospital between June 2020 and January *** status was evaluated by^(13)C-urease breath test 6 weeks after *** adverse events(AEs)during treatment were *** In total,401 cases were ***-one cases were excluded due to loss to follow-up,lack of re-examination,or unwillingness to take *** total of 380 included cases comprised 250 who received VA dual therapy as first-line treatment and 130 who received VA dual therapy as rescue *** was successfully eradicated in 239 cases(95.6%)in the first-line treatment group and 116 cases(89.2%)in the rescue treatment *** overall incidence of AEs was 9.5%for both ***,9.2%of patients experienced an AE in the first-line treatment group and 10.0%in the rescue treatment *** patients discontinued treatment due to AE,with a discontinuation rate of 1.3%.No serious AE *** The VA dual therapy regimen as a first-line treatment and a rescue therapy was effective and safe for elderly patients aged 60 and older.

关键词： Helicobacter pylori Vonoprazan Amoxicillin Dual therapy Elderly