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Identification and characterization of two novel cathelicidins from the frog Odorrana livida

Zoological Research 2019年第2期40卷 94-101页

作者： Ruo-Han Qi Yan Chen Zhi-Lai Guo Fen Zhang Zheng Fang Kai huang Hai-Ning Yu yi-Peng Wang College of Pharmaceutical Sciences Soochow University School of Biology & Basic Medical Sciences Medical College Soochow University Department of Bioscience and Biotechnology Dalian University of Technology

Antimicrobial peptides(AMPs) are a group of gene-encoded small peptides that play pivotal roles in the host immune system of multicellular *** are an important family of AMPs that exclusively exist in vertebrates. Many cathelicidins have been identified from mammals, birds, reptiles and fish. To date, however, cathelicidins from amphibians are poorly understood. In the present study, two novel cathelicidins(OL-CATH1 and 2) were identified and studied from the odorous frog Odorrana ***, the cDNAs encoding the OL-CATHs(780 and735 bp in length, respectively) were successfully cloned from a lung cDNA library constructed for the frog. Multi-sequence alignment was carried out to analyze differences between the precursors of the OL-CATHs and other representative *** peptide sequences of OL-CATH1 and 2 were predicted(33 amino acid residues) and their secondary structures were determined(OL-CATH1 showed a random-coil conformation and OL-CATH2 demonstrated α-helical conformation). Furthermore,OL-CATH1 and 2 were chemically synthesized and their in vitro functions were determined. Antimicrobial and bacterial killing kinetic analyses indicated that OL-CATH2 demonstrated relatively moderate and rapid antimicrobial potency and exhibited strong anti-inflammatory activity. At very low concentrations(10 μg/mL), OL-CATH2 significantly inhibited the lipopolysaccharide(LPS)-induced transcription and production of pro-inflammatory cytokines TNF-α, IL-1βand IL-6 in mouse peritoneal macrophages. In contrast, OL-CATH1 did not exhibit any detectableantimicrobial or anti-inflammatory activities. Overall,identification of these OL-CATHs from O. livida enriches our understanding of the functions of cathelicidins in the amphibian immune system. The potent antimicrobial and anti-inflammatory activities of OL-CATH2 highlight its potential as a novel candidate in anti-infective drug development.

关键词： Antimicrobial peptides (AMPs) Cathelicidins Odorrana livida OL-CATHs Antimicrobial activity Anti-inflammatory activity

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水平圆管内磁性潜热型功能流体对流换热特性的数值模拟

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计算物理 2012年第1期29卷 87-94页

作者：黄勇宣益民李强南京理工大学能源与动力工程学院江苏南京210094

建立磁场作用下水平圆管内磁性潜热型功能流体对流换热的数学物理模型,分析磁场强度、磁性相变微胶囊体积分数、流体质量流量等因素对流体对流换热的影响.磁场对磁性潜热型功能流体的对流换热具有显著的强化作用,磁场强度愈大强化作用... 详细信息

建立磁场作用下水平圆管内磁性潜热型功能流体对流换热的数学物理模型,分析磁场强度、磁性相变微胶囊体积分数、流体质量流量等因素对流体对流换热的影响.磁场对磁性潜热型功能流体的对流换热具有显著的强化作用,磁场强度愈大强化作用愈明显,强化原因是磁性相变微胶囊受到磁力作用产生扰动.

关键词：磁性潜热型功能流体磁场对流换热磁性相变微胶囊

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Dental plaque-inspired versatile nanosystem for caries prevention and tooth restoration

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Bioactive Materials 2023年第2期20卷 418-433页

作者： Yue Xu Yuan You Luyao yi Xiaoyi Wu Yaning Zhao Jian Yu He Liu Ya Shen Jingmei Guo Cui huang The State Key Laboratory Breeding Base of Basic Science of Stomatology(Hubei-MOST)&Key Laboratory for Oral Biomedical Ministry of Education School&Hospital of StomatologyWuhan UniversityWuhanChina Division of Endodontics Faculty of DentistryThe University of British Columbi1Canada

Dental caries is one of the most prevalent human diseases resulting from tooth demineralization caused by acid production of bacteria *** remains challenges for current practice to specifically identify,intervene and interrupt the development of caries while restoring *** this study,inspired by natural dental plaque,a stimuli-responsive multidrug delivery system(PMs@NaF-SAP)has been developed to prevent tooth decay and promote enamel *** spherical core-shell structures of micelles dual-loaded with antibacterial and restorative agents are self-assembled into bacteria-responsive multidrug delivery system based on the pH-cleavable boronate ester bond,followed by conjugation with salivary-acquired peptide(SAP)to endow the nanoparticle with strong adhesion to tooth *** constructed PMs@NaF-SAP specifically adheres to tooth,identifies cariogenic conditions and intelligently releases drugs at acidic pH,thereby providing antibacterial adhesion and cariogenic biofilm resistance,and restoring the microarchitecture and mechanical properties of demineralized *** treatment with PMs@NaF-SAP effectively diminishes the onset and severity of caries without impacting oral microbiota diversity or surrounding mucosal *** findings demonstrate this novel nanotherapy has potential as a promising biomedical application for caries prevention and tooth defect restoration while resisting biofilm-associated diseases in a controlled manner activated by pathological bacteria.

关键词： Biofilms Dental caries Micelles Peptide Stimuli responsive polymers Tooth remineralization

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基于DNS流量的多层多域名检测与测量

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浙江大学学报（工学版） 2020年第12期54卷 2423-2429,2436页

作者：张伊璇龚俭东南大学网络空间安全学院江苏南京211100

为了研究DNS流量中的域名角色,为域名影响力分析提供一种域名定位和筛选的思路,设计一种基于DNS流量的多层多域名检测算法.在检测阶段,从CERNET主干网边界采集DNS流量,提取请求和应答序列.基于多层多域名的聚合特征及解析的并发性,检测... 详细信息

为了研究DNS流量中的域名角色,为域名影响力分析提供一种域名定位和筛选的思路,设计一种基于DNS流量的多层多域名检测算法.在检测阶段,从CERNET主干网边界采集DNS流量,提取请求和应答序列.基于多层多域名的聚合特征及解析的并发性,检测流量中存在的主从域名集合,并引入时间滑动窗口机制进行置信度测量.在测量阶段,对算法检测结果从多个角度进行分析,包括多层多域名集合的规模和相交情况、主从域名的标签级数、集合中从域名对应的资源类型等,并提供了2个存在多层多域名的典型网站案例.测量结果验证了多层多域名现象的存在以及多层多域名集合的特点,表明了此多层多域名检测算法的有效性.

关键词：多层多域名网络测量域名监测网站

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EUS for choosing best endoscopic treatment of mesenchymal tumors of upper gastrointestinal tract

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World Journal of Gastroenterology 2011年第13期17卷 1766-1771页

作者： Xin-Xin Zhou Feng Ji Liang Xu Lin Li yi-Peng Chen Jing-Jing Lu Chun-Wei Wang Wei huang Department of Gastroenterology the First Affiliated HospitalCollege of Medicine Zhejiang University Hangzhou 310003Zhejiang Province China Department of Gastroenterology Beilun ZongruiHospital Ningbo 315806 Zhejiang Province China Department of Gastroenterology the Tradi-tional Chinese Medicine Hospital of Ninghai Ninghai 315600Zhejiang Province China Department of Gastroenterology Jinhua WenrongHospital Jinhua 321017 Zhejiang Province China

AIM: To evaluate the value of endoscopic ultrasonography (EUS) in the choice of endoscopic therapy strategies for mesenchymal tumors of the upper gastrointestinal tract. METHODS: From July 2004 to September 2010, 1050 patients with upper gastrointestinal mesenchymal tumors (GIMTs) were diagnosed using EUS. Among them, 201 patients underwent different endoscopic therapies based on the deriving layers, growth patterns and lesion sizes. RESULTS: Using EUS, we found 543 leiomyomas and 507 stromal tumors. One hundred and thirty-three leiomyomas and 24 stromal tumors were treated by snare electrosection, 6 leiomyomas and 20 stromal tumorswere treated by endoloop, 10 stromal tumors were treated by endoscopic mucosal resection and 8 stromal tumors were treated by endoscopic submucosal dissection. Complete resection of the lesion was achieved in all cases. Of the mesenchymal tumors, 90.38% diagnosed by EUS were also identified by pathohistology. All wounds were closed up nicely and no recurrence was found in the follow-up after 2 mo. CONCLUSION: EUS is an effective means of diagnosis for upper GIMTs and is an important tool in choosing the endoscopic therapy for GIMTs, by which the lesions can be treated safely and effectively.

关键词： Leiomyoma Stromal tumor Endoscopic ultrasonography Endoscopic therapy

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错位双层膜结构全光谱减反射特性研究

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工程热物理学报 2017年第2期38卷 277-280页

作者：郑立凯宣益民南京航空航天大学能源与动力学院南京210016

随着光伏-热电耦合系统的提出,具有宽光谱减反射特性的表面微结构成为太阳能高效利用的关键。本文基于传统TiO_2/SiO_2减反射膜,结合硅纳米柱,提出一种错位膜层复合结构,并从电磁场理论出发,运用时域有限差分方法研究其光谱反射特性,并... 详细信息

随着光伏-热电耦合系统的提出,具有宽光谱减反射特性的表面微结构成为太阳能高效利用的关键。本文基于传统TiO_2/SiO_2减反射膜,结合硅纳米柱,提出一种错位膜层复合结构,并从电磁场理论出发,运用时域有限差分方法研究其光谱反射特性,并分析了结构参数对光谱特性的影响。研究结果表明,通过调节复合结构特征参数,可以得到具有宽光谱特性减反射结构,结构平均反射率约为0.032。

关键词：全光谱减反射缓变折射率微结构

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低浓度猪链球菌溶血素诱导猪肾细胞凋亡方式研究

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农业生物技术学报 2024年第2期32卷 355-366页

作者：戴世馨赵勤徐魁牟欣然杜森焱伍锐文翼平黄小波颜其贵曹三杰四川农业大学动物医学院/猪病研究中心/国家级动物类实验教学示范中心/农业农村部兽用药物与兽医诊断技术四川科学观测站

猪链球菌(Streptococcus suis)是一种危害严重的人畜共患病原菌，猪链球菌溶血素(suilysin, Sly)在猪链球菌的侵袭和致病中起关键作用，对多种类型的细胞具有毒性作用，但目前关于Sly的细胞毒性机制研究较少。为了探讨低浓度(0.3～0.6μ... 详细信息

猪链球菌(Streptococcus suis)是一种危害严重的人畜共患病原菌，猪链球菌溶血素(suilysin, Sly)在猪链球菌的侵袭和致病中起关键作用，对多种类型的细胞具有毒性作用，但目前关于Sly的细胞毒性机制研究较少。为了探讨低浓度(0.3～0.6μg/mL) Sly诱导猪肾细胞PK-15(porcine kidney-15)的死亡方式，本研究首先采用原核表达并纯化获得重组猪链球菌溶血素(recombinant suilysin, rSly)，通过乳酸脱氢酶(lactate dehydrogenase, LDH)释放检测rSly对PK-15细胞的毒性作用，并针对4种常见程序性细胞死亡方式，利用qPCR、间接免疫荧光、Werstern blot和流式细胞术对rSly作用于PK-15细胞12及24 h诱导的细胞死亡方式进行验证。结果显示，成功表达具有较高溶血活性(溶血单位为214)的rSly毒素蛋白，确定其对PK-15细胞的毒性作用呈浓度依赖性，并且发现rSly作用PK-15细胞12和24 h均引起PK-15细胞凋亡相关基因—半胱氨酸天冬氨酸蛋白酶3(cysteine aspartic acid specific protease 3, Caspase 3)、Caspase 9的转录水平显著升高(P<0.05)，细胞凋亡率与r Sly浓度呈正相关，0.6μg/mL rSly作用PK-15细胞24 h，细胞凋亡率从9.35%增至49.9%；自噬关键基因—微管相关蛋白轻链3B(microtubule associated protein 1 light chain 3β, LC3β)基因转录水平无明显变化且无自噬小体形成；焦亡关键基因—胃泌素D(gasdermin D,GSDMD)的转录水平无明显变化且GSDMD蛋白未发生明显切割；坏死性凋亡相关基因下调，且磷酸化混合系列蛋白激酶样结构域蛋白(phosphorylated mixed lineage kinase domain-like protein, p-MLKL)表达水平受到抑制。上述结果提示低浓度rSly诱导PK-15细胞死亡的方式主要是凋亡。本研究丰富了Sly毒性作用的细胞模型，有助于揭示参加猪链球菌感染发展的分子机制。

关键词：重组猪链球菌溶血素(rSly) 猪肾细胞(PK-15) 细胞毒性细胞死亡方式细胞凋亡

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External use of Ruyanneixiao cream efficiently blocks precancerous mammary lesions by interfering with glycolysis induced by inhibition of hypoxia inducible factor-1α, hexokinase 2, phosphofructokinase, and pyruvate kinase M2 expression

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Journal of Traditional Chinese Medicine 2017年第2期37卷 236-243页

作者： Li Xiaobo Ma Min Zhang Guijuan Ma yi Liao Rui Chen Ruixue Yan Xianxin Bie Fengjie huang Maojie Liang Shijie College of Traditional Chinese Medicine Jinan University Guangzhou510632 China the School Outpatient Department TheFirst Affiliated Hospital of Jinan University Guangzhou510632 China Bio-Engineering Institute of Jinan University Guangzhou 510632 China

OBJECTIVE:To investigate the effect of Ruyanneixiao cream(RYNX) on the expression of hypoxia inducible factor-1α(HIF-1α), hexokinase 2(HK2),phosphofructokinase(PFK), and pyruvate kinase M2(PKM2) mRNA and protein in MCF-10 AT cells and in an animal model of precancerous mammary ***:Following treatment of MCF-10 AT cells with RYNX, tamoxifen(TAM) and YC-1 for 48 h,HIF-1α, HK2, PFK, PKM2 mRNA and protein expression was *** female SD rats were randomly divided into control, model, TAM, and highand low-dose RYNX groups, with 10 rats in each group.A precancerous mammary lesion model was established for all groups except the control ***-and low-dose RYNX cream containing TAM was coated on the breasts of animals in the corresponding *** rat mammary tissue was removed in the 10 th week and HIF-1α, HK2, PFK,PKM2 mRNA and protein was ***:In vitro analyses demonstrated that, compared with the matrix group, HIF-1α, HK2, PFK,PKM2 mRNA and protein expression was significantly decreased in the RYNX group(P < 0.05).Compared with the YC-1 + RYNX group, HK2, PFK,and PKM2 protein expression was significantly reduced in the RYNX ***-1α, HK2, PFK, and PKM2 protein expression was increased significantly in the model group(P < 0.05) compared with the control group, while HIF-1α, HK2, PFK, and PKM2 mRNA and protein expression was significantly decreased in both the high-and low-dose RYNX groups(P < 0.05), with the effect being greater in the high-dose ***:RYNX can block precancerous breast lesions by decreasing the expression of HK2,PFK, and PKM2 mRNA and protein via inhibition of HIF-1α mRNA and protein overexpression in a dose-dependent manner.

关键词： Ruyanneixiao cream Precancerous mammary lesions Glycolysis Hypoxia-inducible factor 1 alpha subunit Hexokinase Phosphofructokinases Pyruvate kinase

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The plant circadian clock regulates autophagy rhythm through transcription factor LUX ARRHYTHMO

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Journal of Integrative Plant Biology 2022年第11期64卷 2135-2149页

作者： Ming-Kang Yang Xiao-Jie Zhu Chu-Min Chen Xu Guo Shu-xuan Xu Ya-Rou Xu Shen-Xiu Du Shi Xiao Bernd Mueller-Roeber Wei huang Liang Chen State Key Laboratory for Conservation and Utilization of Subtropical Agro‐Bioresources College of Life SciencesSouth China Agricultural UniversityGuangzhou 510642China Guangdong Laboratory for Lingnan Modern Agriculture Guangzhou 510642China Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms South China Agricultural UniversityGuangzhou 510642China State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources School of Life SciencesSun Yat‐Sen UniversityGuangzhou 510275China Department of Molecular Biology Institute of Biochemistry and BiologyUniversity of Potsdam14476 Potsdam‐GolmGermany Max Planck Institute of Molecular Plant Physiology 14476 Potsdam‐GolmGermany

Autophagy is an evolutionarily conserved degradation pathway in eukaryotes;it plays a critical role in nutritional stress *** circadian clock is an endogenous timekeeping system that generates biological rhythms to adapt to daily changes in the *** evidence indicates that the circadian clock and autophagy are intimately interwoven in ***,the role of the circadian clock in regulating autophagy has been poorly elucidated in ***,we show that autophagy exhibits a robust circadian rhythm in both light/dark cycle(LD)and in constant light(LL)in ***,autophagy rhythm showed a different pattem with a phase-advance shift and a lower amplitude in LL compared to ***,mutation of the transcription factor LUX ARRHYTHMO(LUX)removed autophagy rhythm in LL and led to an enhanced amplitude in *** represses expression of the core autophagy genes ATG2,ATG8 a,and ATG11 by directly binding to their *** analysis revealed that LUX is responsible for improved resistance of plants to carbon starvation,which is dependent on moderate autophagy *** transcriptomic analysis revealed that the autophagy rhythm is ubiquitous in *** together,our findings demonstrate that the LUXmediated circadian clock regulates plant autophagy rhythms.

关键词： autophagy carbon starvation circadian clock LUX rhythm

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杉木-阔叶树复层混交林凋落物和土壤养分及化学计量特征

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浙江林业科技 2024年第4期44卷 1-6页

作者：姜年春邱勇斌张能军宣铃娟郑伟健黄淦胡卫明开化县林场浙江开化324300 开化县林业发展有限公司浙江开化324300 开化县林业局浙江开化324300

杉木纯林改造为杉木-阔叶树复层混交林,对提高杉木林质量具有重要意义,但林分结构改变如何影响凋落物和土壤养分化学计量特征尚缺乏系统研究。以浙西南不同杉木Cunninghamia lanceolata-阔叶树复层混交林为研究对象,分析了杉木纯林、杉... 详细信息

杉木纯林改造为杉木-阔叶树复层混交林,对提高杉木林质量具有重要意义,但林分结构改变如何影响凋落物和土壤养分化学计量特征尚缺乏系统研究。以浙西南不同杉木Cunninghamia lanceolata-阔叶树复层混交林为研究对象,分析了杉木纯林、杉木+柃木Eurya japonica、杉木+蚊母树Distylium racemosum及杉木+闽楠Phoebe bournei混交林凋落物和0~40 cm土层土壤碳、氮、磷含量及其化学计量特征。结果表明:相对于杉木纯林,杉木+柃木混交林凋落物有机碳含量显著降低,杉木+闽楠凋落物总磷含量显著增加,不同林分类型之间凋落物碳、氮、磷化学计量比变异相对较小。林分类型和土层显著影响土壤养分含量,杉木-阔叶树混交林土壤养分含量普遍高于杉木纯林,0~20 cm土层的平均土壤有机碳、总氮和总磷含量分别比杉木纯林增加25.2%、48.8%和50.0%,20~40 cm土层的平均土壤有机碳、总氮和总磷含量分别比杉木纯林增加26.9%、45.4%和47.6%。林分类型显著影响土壤碳磷比和氮磷比,并与凋落物化学计量显著正相关。因此,将杉木纯林转换为杉木复层混交林有助于改善土壤养分状况,但改良效果取决于树种组成,研究结果对加强杉木阔叶树复层混交林经营管理具有重要意义。

关键词：杉木混交林凋落物土壤养分化学计量

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