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Single-nucleotide polymorphisms in HORMAD1 may be a risk factor for azoospermia caused by meiotic arrest in Japanese patients

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Asian Journal of Andrology 2012年第4期14卷 580-583页

作者： Toshinobu Miyamoto Aldra Tsujimura Yasushi Miyagawa Eitetsu Koh Mikio Namiki Michiharu Horikawa yasuaki saijo Kazuo Sengoku Department of Obstetrics and Gynecology Asahikawa Medical University Asahikawa Hokkaido 078-8510 Japan Department of Urology Osaka University Graduate School of Medicine Suita 565-0871 Japan Department of Integrated Cancer Therapy and Urology Kanazawa University Graduate School of Medical Science Kanazawa 920-1192 Japan Division of Community Medicine and Epidemiology Department of Health Science Asahikawa Medical University Asahikawa 078-8510 Japan

Genetic mechanisms are implicated as a cause of some male infertility, yet are poorly understood. Meiosis is unique to germ cells and essential for reproduction. The synaptonemal complex is a critical component for chromosome pairing, segregation and recombination. Hormadl is essential for mammalian gametogenesis as knockout male mice are infertile. Hormadl-deficient testes exhibit meiotic arrest in the early pachytene stage and synaptonema! complexes cannot be visualized. To analyze the hypothesis that the human HORMAD1 gene defects are associated with human azoospermia caused by meiotic arrest, mutational analysis was performed in all coding regions by direct sequence analysis of 30 Japanese men diagnosed with azoospermia resulting from meiotic arrest. By the sequence analysis, three polymorphism sites, Single Nucleotide Polymorphism 1 （c. 163A〉G）, SNP2 （c. 501T〉G） and SNP3 （c. 918C〉T）, were found in exons 3, 8 and 10. The 30 patients with azoospermia and 80 normal pregnancy-proven, fertile men were analyzed for HORMAD1 polymorphisms. Both SNP1 and SNP2 were associated with human azoospermia caused by complete early meiotic arrest （P〈0.0S）. We suggest that the HORMAD1 has an essential meiotic function in human spermatogenesis.

关键词： azoospermia HORMAD1 male infertility male meiosis SNP

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An association study of the single-nucleotide polymorphism c190C〉T （Arg64Cys） in the human testis-specific histone variant, H3t, of Japanese patients with Sertoli cell-only syndrome

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Asian Journal of Andrology 2018年第5期20卷 527-528页

作者： Toshinobu Miyamoto Masashi Iijima Takeshi Shin Gaku Minase Hiroto Ueda yasuaki saijo Hiroshi Okada Kazuo Sengoku Department of Obstetrics and Gynecology Asahikawa Medical University Asahikawa 078-8510 Japan Department of Urology Kanazawa University Graduate School of Medical Science Kanazawa 920-8641 Japan Department of Urology Dokkyo Medical University Koshigaya Hospital Koshigaya 343-8555 Japan Division of Public Health and Epidemiology Department of Social Medicine Asahikawa Medical University Asahikawa 078-8510 Japan

Dear Editor,Approximately 20% of men with nonobstructive azoospermia （NOA） are diagnosed with infertility caused by genetic defects.1 These include chromosomal abnormalities, Y-chromosome microdeletions, and several specific gene mutations/deletions, such as in DAZ, RBMY, USP9Y, SYCP3, HSF2, PLK4, and TEX11.1,2 Several histones have been detected in mammalian testes, and testis-specific variants are specifically and highly expressed during spermatogenesis^3.

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H pylori seropositivity and cytokine gene polymorphisms

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World Journal of Gastroenterology 2007年第33期13卷 4445-4451页

作者： yasuaki saijo Eiji Yoshioka Tomonori Fukui Mariko Kawaharada Fumihiro Sata Hirokazu Sato Reiko Kishi Department of Health Science Asahikawa Medical College Midorigaoka E2-1-1-1 Asahikawa Hokkaido 078-8510 Japan Department of Public Health Hokkaido University Graduate School of Medicine Kita 15 Nishi 7 Kita-ku Sapporo 060-8638 Japan Health Administration Department Sapporo Railway Hospital Kita 3 Higashi 1 Cyuo-ku Sapporo 060-0033Japan

AIM: To investigate whether the pro- and anti-inflammatory cytokine gene polymorphisms, IL1B-511C/T, IL1B-31C/T, IL6-634C/G, TNF-1031T/C, TNF-857C/T, and IL10-1082A/G, interact with smoking and drinking habits to influence infection with H ***: The subjects were 410 Japanese transit company employees. C-reactive protein and conventional cardiovascular risk factors were evaluated. Serum anti-H pylori antibodies were measured. The genotypes of IL1B-511C/T, IL1B-31C/T, IL6-634C/G, TNF-1031T/C, TNF-857C/T, and IL10-1082A/G polymorphisms were determined by allelic discrimination using fluorogenic probes and a 5′nuclease ***: In gender- and age-adjusted logistic analyses, the subjects with TNF-857T/T had a significantly lower odds ratio (OR) for H pylori seropositivity (reference -857C/C; OR = 0.15, 95% CI: 0.03-0.59, P = 0.007). After stratification according to smoking and drinking status, among never-smokers, the subjects with IL1B-511C/T had a significantly lower OR (reference -511C/C; OR = 0.30, 95% CI: 0.10-0.90, P = 0.032). Among drinkers in the 1-5 times/wk category, the subjects with IL1B-511T/T had a significantly lower OR (reference C/C; OR = 0.38, 95% CI: 0.16-0.95, P = 0.039), and the subjects with IL1B-31C/T and T/T had a significantly higher OR (reference C/C; C/T: OR = 2.59, 95% CI, P = 0.042: 1.04-6.47; C/C: OR = 3.17, 95% CI: 1.23-8.14, P = 0.017). Among current smokers, the subjects withIL6-634C/G had a significantly higher OR (reference C/C; OR = 2.28, 95% CI: 1.13-4.58, P = 0.021). However, the interactions terms between the aforementioned genotypes and lifestyles were not statistically ***: Contrary to previous findings, the results herein suggest that the TNF-857T/T genotype may be protective against chronic infection with H pylori. Drinking and smoking habits may influence the effect of cytokine gene polymorphisms. Further studies are required to clarify the effects of the pro- and anti-inflammatory cytokine

关键词：细胞活素类细胞基因医学研究

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Light chain cardiac amyloidosis in a nonagenarian

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Journal of Geriatric Cardiology 2022年第1期19卷 83-89页

作者： Koji Takahashi Mina Yamashita Tomoki Sakaue Daijiro Enomoto Shigeki Uemura Takafumi Okura Shuntaro Ikeda Takanori Senba Akira saijo Nobuhisa Yamamura Sohei Kitazawa Department of Community Emergency Medicine Ehime University Graduate School of MedicineEhimeJapan Department of Cardiology Yawatahama City General HospitalEhimeJapan Department of Hematology Yawatahama City General HospitalEhimeJapan Department of Clinical Pathology Yawatahama City General HospitalEhimeJapan Department of Molecular Pathology Ehime University Graduate School of MedicineEhimeJapan

Cardiac amyloidosis is an infiltrative and restrictive cardiomyopathy caused by the extracellular deposition of amyloid fib-rils within the heart as systemic amyloidosis,lead-ing to heart failure,reduced quality of life,and death.[1]There are two major amyloid fibril proteins that af-fect the heart:amyloid immunoglobulin light chain(AL)and amyloid transthyretin(ATTR).The latter is further subdivided into wild-type ATTR and variant types based on the presence of a mutation in the transthyretin gene.

关键词： amyloid death. cardiomyopathy

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Involvement of Dectin-2 in the Innate Inflammatory Response Triggered by Influenza Virus Hemagglutinin

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Advances in Infectious Diseases 2023年第3期13卷 478-497页

作者： Hideki Yamamoto Chikako Tomiyama Sho Yamasaki Shinobu saijo Yoichiro Iwakura Kazuyoshi Kawakami Department of Medical Technology Graduate School of Health Sciences Niigata University Niigata Japan Department of Molecular Immunology Research Institute for Microbial Diseases Osaka University Osaka Japan Laboratory of Molecular Immunology Immunology Frontier Research Center Osaka University Osaka Japan Project for Cytokine Research Division of Molecular Immunology Medical Mycology Research Center Chiba University Chiba Japan Center for Animal Disease Models Research Institute for Biomedical Sciences Tokyo University of Science Chiba Japan Department of Medical Microbiology Mycology and Immunology Tohoku University Miyagi Japan Department of Internal Medicine Hirose Hospital Miyagi Japan

C-type lectin receptors (CLRs) are representative pattern recognition receptors that recognize microbial polysaccharides expressed on antigen-presenting cells. In the present study, we carried out further detailed analysis on the involvement of Dectin-2, a CLR that senses high mannose polysaccharide, in innate immune responses induced by influenza virus hemagglutinin (HA). Treatment of HA with periodate or PNGase F induced lower interleukin (IL)-12p40 secretion by conventional dendritic cells (DCs) compared with the untreated group. In contrast, treatment with O-glycosidase did not affect cytokine production. Green fluorescent protein expression in canonical Dectin-2-transducing cells was approximately 3% - 12% following HA stimulation, except with the A/H1N1pdm09 subtype HA. This expression was markedly reduced in cells possessing mutated amino acids in the carbohydrate recognition domain of Dectin-2, especially following stimulation with HA derived from the A/H3N2 subtype. Interferon (IFN)-α production from CD11c+Siglec-H+PDCA-1+ plasmacytoid DCs was significantly increased in Dectin-2 knockout mice compared with wild-type mice upon stimulation with HA except for the B/Yamagata lineage HA. These results suggested that Dectin-2 is involved in initiating inflammatory responses via mannose polysaccharide on HA. However, other mechanisms may function in the antiviral response, including the type I IFN axis.

关键词： C-Type Lectin Receptors Influenza Virus Innate Immunity Type I Interferon

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ENVIRONMENTAL FRIENDLY ANODIZING ON AZ91D MAGNESIUM ALLOYS AND COATING CHARACTERISTICS

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Acta Metallurgica Sinica(English Letters) 2005年第3期18卷 411-415页

作者： A. saijo M. Hino M. Hiramatsu T. Kanadani Hori Metal Finishing Industry Co. Ltd. Abe Ochiai-cho Takahashi-shi Okayama 716-0061 Japan Industrial Technology Research Institute of Okayama Prefecture Haga Okayama-sh i Okayama 701-1296 Japan Industrial Technology Research Institute of Okayama Prefecture Haga Okayama-sh i Okayama 701-1296 Japan Graduate School of Okayama University of Science Ridai-cho Okayama-shi Okayam a 700-0005 Japan

An environmental friendly anodizing treatment (Anomag) from a phosphate-based so lution without heavy metals on AZ91D magnesium alloy was studied. The characteri stics of the coatings, such as structure, composition and corrosion resistance w ere investigated. The effects of this anodizing treatment on the mechanical prop erties were examined. X-ray diffraction (XRD) analysis revealed that the structu re of the coatings is amorphous or glassy. In salt spray tests coatings with an average thickness of 10μm had an anticorrosive performance of over 1000 hours. Fatigue tests revealed that anodizing onto AZ91D magnesium alloy does not affect the fatigue strength. These results demonstrate the utility of this anodizing t reatment on magnesium alloy for application as a structural material, such as in the automotive field.

关键词： magnesium alloy anodizing environmental friendliness

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ELECTROPLATED Zn-Ni-SiO_2 COMPOSITE COATINGS TREATED WITH A SILANE COUPLING AGENT TO REPLACE CHROMATING

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Acta Metallurgica Sinica(English Letters) 2005年第3期18卷 416-422页

作者： M. Hino M. Hiramatsu K. Murakami A. saijo T. Kanadani Industrial Technology Research Institute of Okayama Prefecture Haga Okayama-sh i Okayama 701-1296 Japan Industrial Technology Research Institute of Okayama Prefecture Haga Okayama-sh i Okayama 701-1296 Japan Industrial Technology Research Institute of Okayama Prefecture Haga Okayama-sh i Okayama 701-1296 Japan Hori Metal Finishing Industry Co.Ltd. Abe Ochiai-cho Takahashi-shi Okayama 716-0061 Japan Graduate School of Okayama University of Science Ridai-cho Okayama-shi Okayam a 700-0005 Japan

The fixing of a silane coupling agent to Zn-Ni-silica (SiO2) composite coatings was studied for the purpose of developing a coating process as an alternative to chromating. The corrosion resistance of Zn-Ni-silica composite coatings was rem arkably improved by the silica nanoparticles in the composite, which were disper sed in the surface of this film. The silane coupling agent formed chemical bonds with the inorganic silica particles during the silane coupling treatment on the se composite coatings. The treatment suppressed the formation of white corrosion products to the same extent as chromating, as measured in salt spray tests. It is concluded that treating Zn-Ni-silica composite coatings with silane coupling agents is a viable alternative technique to chromating.

关键词： electrodeposition Zn-Ni-SiO2 composite coating silane coupling

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Application of Scanning Acoustic Microscopy for Detection of Dental Caries Lesion

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Open Journal of Stomatology 2023年第1期13卷 12-24页

作者： Yukihiro Naganuma Kouki Hatori Masahiro Iikubo Masatoshi Takahashi Yoshihiro Hagiwara Kazuto Kobayashi Atsushi Takahashi Kumi Hoshi Yoshifumi saijo Keiichi Sasaki Clinics of Dentistry for Disabled Tohoku University Hospital Sendai Japan Department of Prosthetic Dentistry Ohu University School of Dentistry Koriyama Japan Division of Dental Informatics and Radiology Tohoku University Graduate School of Dentistry Sendai Japan Division of Dental Biomaterials Tohoku University Graduate School of Dentistry Sendai Japan Department of Orthopaedic Surgery Tohoku University School of Medicine Sendai Japan Honda Electronics Co. Ltd. Toyohashi Japan Biomedical Imaging Laboratory Graduate School of Biomedical Engineering Tohoku University Sendai Japan Division of Advanced Prosthetic Dentistry Tohoku University Graduate School of Dentistry Sendai Japan

Introduction: A scanning acoustic microscope (SAM) is an apparatus for imaging acoustic properties. This apparatus can non-invasively and rapidly evaluate the hardness of materials in the elastic region. This device shows great potential for the diagnosis of dental caries in the clinical setting. However, since the tissue elastic modulus measured using a SAM is a property of the elastic region and the Knoop hardness is a property of the plastic region, the hardness properties differ completely. Therefore, we investigated whether the acoustic impedance measured using a SAM is related to the Knoop hardness, which is used as the standard for removal of carious dentin. Method: Polished sections were prepared from 20 extracted carious wisdom teeth. The acoustic impedance and Knoop hardness were measured for each section. In addition to comparing carious and healthy dentin in SAM images, we evaluated the difference between the carious and healthy dentin in terms of the acoustic impedance and Knoop hardness. We also evaluated the correlation between the Knoop hardness and acoustic impedance. Results: The SAM images were visualized as two-dimensional color images based on the acoustic impedance values. The mean acoustic impedance of carious dentin was significantly lower than that of healthy dentin, showing a similar trend as Knoop hardness. A strong correlation was observed between the two. Discussion: The acoustic impedance values obtained through acoustic microscopy differed significantly between carious and sound dentin. Both types of dentins were visualized using two-dimensional color images. A strong correlation was observed between the acoustic impedance value, which indicates the hardness of the elastic region, and the Knoop hardness, which indicates the hardness of the plastic region. The results of the present study indicate that acoustic impedance accurately reflects the hardness of dentin.

关键词： Scanning Acoustic Microscope Acoustic Impedance Caries Knoop Hardness

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Biomedical Application of Acoustic Microscopy-Diagnosis,Assessing Echogenecity and Biomechanics

Biomedical Application of Acoustic Microscopy-Diagnosis,Asse...

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2008 IEEE International Ultrasonics Symposium

作者： Yoshifumi saijo Tohoku University Department of Biomedical ImagingSendai

＜正＞Background,Motivation and Objective SAM technology has been applied in medical and biological studies for 1)intra-operative pathological examinations since it does not require special staining techniques,2)deter...

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Inactivation of severe fever with thrombocytopenia syndrome virus for improved laboratory safety

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Journal of Biosafety and Biosecurity 2020年第1期2卷 31-35页

作者： Toshihiko Harada Shuetsu Fukushi Takeshi Kurosu Tomoki Yoshikawa Masayuki Shimojima Kiyoshi Tanabayashi Masayuki saijo Division of Biosafety Control and Research National Institute of Infectious DiseasesTokyoJapan Department of Virology 1 National Institute of Infectious DiseasesTokyoJapan

Severe fever with thrombocytopenia syndrome(SFTS),a tick-borne infectious disease with high mortality,is diagnosed by the serological testing of serum samples from patients in the acute and convalescent phases and/or by direct detection of the SFTS virus(SFTSV).Conventionally,heat and UV treatments have been used to inactivate the viruses present in serum samples before performing the serological ***,we examined the inactivation conditions optimal for SFTSV-containing serum samples to ensure the safety of laboratory workers while maintaining the accuracy of serological assay results *** human serum samples spiked with SFTSV to 60C for 30 min or exposing them to UV irradiation for 30 min,reduced the infectious virus titer below the limit of *** in sera from patients in the acute phase of SFTS was completely inactivated by heating to 60C for 30 min,followed by UV irradiation for 10 *** inactivation procedure had minimal impact on the performance of SFTSV antibody detection *** data provided herein can serve as a guide for laboratory workers and researchers working with SFTS serological tests.

关键词： Biosafety Inactivation Severe fever with thrombocytopenia syndrome(SFTSV) Serology

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