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Rapid full-color Fourier ptychographic microscopy via spatially filtered color transfer

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Photonics Research 2022年第10期10卷 2410-2421页

作者： JIURUN CHEN AIYE WANG AN PAN GUOAN ZHENG CAIWEN MA baoli yao Xi’an Institute of Optics and Precision Mechanics Chinese Academy of SciencesXi’an 710119China University of Chinese Academy of Sciences Beijing 100049China CAS Key Laboratory of Space Precision Measurement Technology Xi’an 710119China Department of Biomedical Engineering University of ConnecticutStorrsConnecticut 06269USA

Full-color imaging is of critical importance in digital pathology for analyzing labeled tissue *** our previous cover story[***:Phys.,***.64,114211(2021)],a color transfer approach was implemented on Fourier ptychographic microscopy(FPM)for achieving high-throughput full-color whole slide imaging without mechanical *** approach was able to reduce both acquisition and reconstruction time of FPM by three-fold with negligible trade-off on color ***,the method cannot properly stain samples with two or more dyes due to the lack of spatial constraints in the color transfer *** also requires a high computation cost in histogram matching of individual *** we report a modified full-color imaging algorithm for FPM,termed color-transfer filtering FPM(CFFPM).In CFFPM,we replace the original histogram matching process with a combination of block processing and trilateral spatial *** former step reduces the search of the solution space for colorization,and the latter introduces spatial constraints that match the low-resolution *** further adopt an iterative process to refine the *** show that this method can perform accurate and fast color transfer for various specimens,including those with multiple *** statistical results of 26 samples show that the average root mean square error is only 1.26%higher than that of the red-green-blue sequential acquisition *** some cases,CFFPM outperforms the sequential method because of the coherent artifacts introduced by dust *** reported CFFPM strategy provides a turnkey solution for digital pathology via computational optical imaging.

关键词： transfer matching constraints

Structured illumination microscopy for super-resolution and optical sectioning

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Chinese Science Bulletin 2014年第12期59卷 1291-1307页

作者： Dan Dan baoli yao Ming Lei State Key Laboratory of Transient Optics and Photonics Xi’an Institute of Optics and Precision Mechanics Chinese Academy of Sciences

Optical microscopy plays an essential role in biological studies due to its capability and compatibility of non-contact,minimally invasive observation and measurement of live ***,the conventional optical microscopy only has a spatial resolution about200 nm due to the Abbe diffraction limit,and also lacks the ability of three-dimensional ***-resolution farfield optical microscopy based on special illumination schemes has been dramatically developed over the last *** them,only the structured illumination microscopy(SIM)is of wide-field geometry that enables it easily compatible with the conventional optical *** this article,the principle of SIM was introduced in terms of point spread function(PSF)and optical transform function(OTF)of the optical *** SIM for super-resolution(SIM-SR)proposed by Gustafsson et *** the SIM for optical sectioning(SIM-OS)proposed by Neil et *** the most popular ones in the research community of *** have the same optical configuration,but with different data postprocessing *** mathematically described the basic theories for both of the SIMs,respectively,and presented some numerical simulations to show the effects of super-resolution and optical *** approaches to generation of structured illumination patterns were *** an example,a SIM system based on DMDmodulation and LED-illumination was demonstrated.A lateral resolution of 90 nm was achieved with gold *** optical sectioning capability of the microscope was demonstrated with Golgi-stained mouse brain neurons,and the sectioning strength of 930 nm was obtained.

关键词：光学显微镜光学切片超分辨率结构照明 SIM卡金纳米粒子成像能力光学传递函数

High-throughput fast full-color digital pathology based on Fourier ptychographic microscopy via color transfer

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Science China(Physics,Mechanics & Astronomy) 2021年第11期64卷 57-68页

作者： Yuting Gao Jiurun Chen Aiye Wang An Pan Caiwen Ma baoli yao Xi’an Institute of Optics and Precision Mechanics Chinese Academy of SciencesXi’an 710119China University of Chinese Academy of Sciences Beijing 100049China

The usage of full-color imaging in digital pathology produces significant *** with a grayscale image or a pseudocolor image containing contrast information,a full-color image can identify and detect the target object better with color texture *** ptychographic microscopy(FPM)is a high-throughput computational imaging technique that breaks the tradeoff between high resolution(HR)and a large field of *** also eliminates the artifacts of scanning and stitching in digital pathology and improves its imaging ***,the conventional full-color digital pathology based on FPM is still time-consuming because of the repeated experiments with tri-wavelengths.A color transfer FPM approach termed“CFPM”was *** color texture information of a low-resolution full-color pathologic image is directly transferred to the HR grayscale FPM image captured by only a single *** of the color space of FPM based on the standard CIE-XYZ color model and the display based on the standard RGB color space were *** FPM colorization schemes were analyzed and compared with 30 biological *** types of evaluation approaches were provided,including the root-mean-square error(RMSE),the difference maps,and the image histogram cosine *** average RMSE values of the conventional method and CFPM compared with the ground truth were 5.3%and 5.7%,***,the reconstruction time is significantly reduced by 2/3 with the sacrifice of precision of only 0.4%.The CFPM method is also compatible with advanced fast FPM approaches to further reduce computation time.

关键词： computational imaging Fourier ptychographic microscopy digital pathology high throughput transfer learning

Off-axis optical levitation and transverse spinning of metallic microparticles

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Photonics Research 2021年第11期9卷 2144-2151页

作者： YANSHENG LIANG SHAOHUI YAN ZHAOJUN WANG baoli yao MING LEI MOE Key Laboratory for Nonequilibrium Synthesis and Modulation of Condensed Matter Shaanxi Province Key Laboratory of Quantum Information and Quantum Optoelectronic DevicesSchool of PhysicsXi’an Jiaotong UniversityXi’an 710049China State Key Laboratory of Transient Optics and Photonics Xi'an Institute of Optics and Precision MechanicsChinese Academy of SciencesXi'an 710119China

Optical manipulation of metallic microparticles remains a significant challenge because of the strong scattering forces arising from the high extinction coefficient of the *** paper reports a new mechanism for stable confinement of metallic microparticles using a tightly focused linearly polarized Gaussian *** and experimental results demonstrate that metallic microparticles can be captured off the optical axis in such a ***,the three-dimensionally confined particles are observed spinning transversely as a response to the asymmetric force *** off-axis levitation and transverse spinning of metallic microparticles may provide a new way for effective manipulation of metallic microparticles.

关键词： particles. field. metallic

Digital holographic shape measurement using Fizeau microscopy

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Chinese Optics Letters 2015年第10期13卷 40-44页

作者： D.G.Abdelsalam Junwei Min Daesuk Kim baoli yao State Key Laboratory of Transient Optics and Photonics Xi'an Institute of Optics and Precision MechanicsChinese Academy of Sciences Engineering and Surface Metrology Laboratory National Institute of Standards Division of Mechanical System Engineering Chonbuk National University

We present a Fizeau interferometer using a microscopic objective as a tool for surface contouring without the need for a numerical lens for reconstruction. The interferometer is associated with a telescope system to feature the object with collimated light. The experiment is conducted on two objects possessing different step *** phase maps from the captured off-axis holograms are calculated numerically, which allows us to deduce the contours of the objects. The great advantages of the presented technique are that it can be done in real time and there is no need for numerical lenses for micro-objects reconstruction.

关键词： interferometer holographic captured reconstructed numerically microscopic Reconstruction compensate wavefront filtered

平场定量相位显微镜在骨髓间充质干细胞线粒体动力学观察中的应用研究(特邀)

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激光与光电子学进展 2024年第6期61卷 308-314页

作者：戴太强马英杜宇轩侯燕吕前欣康娟姚保利郜鹏孔亮口颌系统重建与再生全国重点实验室陕西西安710032 国家口腔疾病临床医学研究中心陕西西安710032 陕西省口腔疾病临床医学研究中心陕西西安710032 第四军医大学口腔医院颌面外科陕西西安710032 西安电子科技大学物理学院陕西西安710171 中国科学院西安光学精密机械研究所瞬态光学与光子技术国家重点实验室陕西西安710119

分离培养SD大鼠骨髓间充质干细胞,传代纯化后接种至共聚焦培养皿,置于自主研发搭建的平场定量相位显微镜下,利用荧光及相位双通道证实线粒体特征后,进行长时间无标记观察,分析平场定量相位显微镜观察到的线粒体分裂、融合过程,以及细胞... 详细信息

分离培养SD大鼠骨髓间充质干细胞,传代纯化后接种至共聚焦培养皿,置于自主研发搭建的平场定量相位显微镜下,利用荧光及相位双通道证实线粒体特征后,进行长时间无标记观察,分析平场定量相位显微镜观察到的线粒体分裂、融合过程,以及细胞凋亡过程中的线粒体变化。平场定量相位显微镜无标记观察到的线粒体可与荧光标记的线粒体完全重叠,表明平场定量相位显微镜可以清晰观察线粒体并进行无标记高分辨率成像。同时,平场定量相位显微镜可以对培养条件下的骨髓间充质干细胞进行长时间无标记观察,并高分辨率记录线粒体分裂、融合过程。此外,还利用平场定量相位显微镜首次完整记录了CCCP作用下线粒体的变化,该变化直观地呈现了线粒体途径的细胞凋亡过程。平场定量相位显微镜可以对培养的细胞进行长时间无标记高分辨率观察,为线粒体动力学的研究提供了一种新的观察手段。

关键词：无标记成像平场定量相位显微镜骨髓间充质干细胞线粒体动力学

Dual-wavelength in-line digital holography with untrained deep neural networks

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Photonics Research 2021年第12期9卷 2501-2510页

作者： CHEN BAI TONG PENG JUNWEI MIN RUNZE LI YUAN ZHOU baoli yao State Key Laboratory of Transient Optics and Photonics Xi’an Institute of Optics and Precision MechanicsChinese Academy of SciencesXi’an 710119China Xi’an Jiaotong University Xi’an 710049China Pilot National Laboratory for Marine Science and Technology(Qingdao) Qingdao 266200China

Dual-wavelength in-line digital holography(DIDH)is one of the popular methods for quantitative phase imaging of objects with non-contact and high-accuracy *** technical challenges in the reconstruction of these objects include suppressing the amplified noise and the twin-image that respectively originate from the phase difference and the phase-conjugated *** contrast to the conventional methods,the deep learning network has become a powerful tool for estimating phase information in DIDH with the assistance of noise suppressing or twin-image removing ***,most of the current deep learning-based methods rely on supervised learning and training instances,thereby resulting in weakness when it comes to applying this training to practical imaging *** this paper,a new DIDH network(DIDH-Net)is proposed,which encapsulates the prior image information and the physical imaging process in an untrained deep neural *** DIDHNet can effectively suppress the amplified noise and the twin-image of the DIDH simultaneously by automatically adjusting the weights of the *** obtained results demonstrate that the proposed method with robust phase reconstruction is well suited to improve the imaging performance of DIDH.

关键词： neural networks network

基于DMD调制的结构光照明超分辨和光切片显微技术研究进展(特邀)

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激光与光电子学进展 2024年第6期61卷 1-24页

作者：马旺千佳王思颖马睿李星党诗沛栗星柏晨但旦姚保利中国科学院西安光学精密机械研究所瞬态光学与光子技术国家重点实验室陕西西安710119 中国科学院大学北京100049

将普通光学显微镜的均匀照明替换为光场具有空间结构分布的照明,可为显微镜增添超分辨和光切片的新功能。结构光照明显微(SIM)技术与传统宽场光学显微镜具有良好的结构兼容性,继承了传统光学显微镜非侵入、低光毒性、低荧光漂白、快速... 详细信息

将普通光学显微镜的均匀照明替换为光场具有空间结构分布的照明,可为显微镜增添超分辨和光切片的新功能。结构光照明显微(SIM)技术与传统宽场光学显微镜具有良好的结构兼容性,继承了传统光学显微镜非侵入、低光毒性、低荧光漂白、快速成像的优点。其高时空分辨率和三维光切片能力非常适合活体细胞或组织的观测,受到生物医学和光学界的持续关注。快速产生高对比度、高频率的结构光场并进行快速相移和旋转调控是SIM的核心技术。近年来基于数字微镜器件(DMD)调制的SIM(DMD-SIM)发展迅速,它利用DMD高刷新率、高光通量、偏振不敏感的优势,克服了传统器件如物理光栅和液晶空间光调制器在调控速度上的缺点。本综述首先介绍了SIM超分辨和光切片的基本原理,然后着重阐述了DMD-SIM通过光投影和光干涉产生结构光照明及调控光场的方法,对当前的DMD-SIM研究进展进行了归纳评述,总结了DMD-SIM的优缺点,最后对DMD-SIM面临的挑战和发展趋势进行了展望。

关键词：光学显微结构光照明显微超分辨光切片数字微镜器件

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超构表面赋能光学微操控技术(特邀)

光学学报 2024年第5期44卷 1-18页

作者：徐孝浩高文禹李添悦邵天骅李星仪周源高歌泽王国玺严绍辉王漱明姚保利中国科学院西安光学精密机械研究所瞬态光学与光子技术国家重点实验室陕西西安710119 中国科学院大学北京100049 南京大学固体微结构物理国家重点实验室南京大学物理学院江苏南京210093 南京大学人工微结构科学与技术协同创新中心江苏南京210093 浙江大学光电科学与工程学院浙江杭州310027

光学超构表面凭借其小型化集成化的优势和对光场出色的调控能力,近年来已被深入应用于光学微操控技术研究,这标志着该交叉领域进入了新的发展阶段。特别地,由于超构表面的尺寸在亚波长级别,具有被光场驱动从而产生机械运动的潜力,这一... 详细信息

光学超构表面凭借其小型化集成化的优势和对光场出色的调控能力,近年来已被深入应用于光学微操控技术研究,这标志着该交叉领域进入了新的发展阶段。特别地,由于超构表面的尺寸在亚波长级别,具有被光场驱动从而产生机械运动的潜力,这一特性为新一代光驱动的人工微机器人提供了重要的理论基础和技术支撑。本文依次从光学微操控的基本原理和超表面的相位机制出发,详细回顾了基于超构表面的多种微操控器件,包括超构表面光镊、多功能微操控系统、超构机械等,并结合微纳结构的拓扑光学性质,对拓扑光操控等新奇效应进行了探讨。最后,本文展望了超构表面微操纵技术的未来发展方向和目标。

关键词：超构表面光学微操控光镊光子力学