检索结果-南通市图书馆

Unraveling the drug distribution in brain enabled by MALDI MS imaging with laser-assisted chemical transfer

在线全文

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2022年第4期12卷 2120-2126页

作者： Shuai Guo Kening Li yanwen chen Bin Li State Key Laboratory of Natural Medicines and School of Traditional Chinese Pharmacy China Pharmaceutical UniversityNanjing 210009China

Accurate localization of central nervous system(CNS)drug distribution in the brain is quite challenging to matrix-assisted laser desorption/ionization(MALDI)mass spectrometry imaging(MSI),owing to the ionization competition/suppression of highly abundant endogenous biomolecules and MALDI ***,we developed a highly efficient sample preparation technique,laser-assisted chemical transfer(LACT),to enhance the detection sensitivity of CNS drugs in brain tissues.A focused diode laser source transilluminated the tissue slide coated with α-cyano-4-hydroxycinnamic acid,an optimal matrix to highly absorb the laser radiation at 405 nm,and a very thin-layer chemical film mainly containing drug molecule was transferred to the acceptor glass ***,MALDI MSI was performed on the chemical film without additional sample *** major advantage of LACT is to minimize ionization competition/suppression from the tissue itself by removing abundant endogenous lipid and protein *** superior performance of LACT led to the successful visualization of regional distribution patterns of 16 CNS drugs in the mouse ***,the dynamic spatial changes of risperidone and its metabolite were visualized over a 24-h ***,the brain-to-plasma(B/P)ratio could be obtained according to MALDI MSI results,providing an alternative means to assess brain penetration in drug discovery.

关键词： Pharmaceutical analysis Laser-assisted chemical transfer Mass spectrometry imaging Drug distribution Brain penetration

An explanation to the high efficiency of m-THPC (tempor-fin) used in photodynamic therapy

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2001年第10期46卷 823-827页

作者： yanwen chen Lei Li Manhua Zhang Tao Shen Chinese Acad Sci Inst Chem Ctr Mol Sci Beijing 100080 Peoples R China

Meso-tetrahydroxylphenyl chlorin (m-THPC) is one of the most efficient prospective sensitizers used in photodynamic therapy (PDT). ESR spectroscopy, fluorescence quenching experiments and cyclic voltammogram measurement were used to study its redox properties. The results showed that the ability of m-THPC generating su-peroxide radical anions was very strong, and the rate constant of m-THPC fluorescence quenching by oxygen kq (O2)=1.46×l010 mol-1 . s-1. The values of fluorescence quenching rate constant of m-THPC by some other electron acceptors, such as methyl viologen (MV2+) and anthraquinone (An), were also measured. And they were kq, (MV2+)=5.51×l09 mol-1 . s-1, kq (An)=7.81×l09 mol-1 .s-1. The oxidation potential of m-THPC was examined to be +0.62 V (vs. NHE) in acetonitrile. All these suggested that m-THPC should be a much stronger electron donor than photofrin, the currently used in clinical photodrug, and may react easily through electron transfer with biological matter to yield various

关键词： meso-tetrahydroxylphenyl chlorin photodynamic therapy redox properties superoxide anion radical fluorescence quenching.

在线全文

学校读者我要写书评

暂无评论

甘肃碧口地区金铜矿地质特征及找矿潜力浅析

西北地质 2019年第1期52卷 206-220页

作者：陈彦文甘肃省地矿局第一地质矿产勘查院甘肃天水741020

碧口地区地处摩天岭地块,是中国重要的金铜成矿远景区、矿集区。笔者以甘肃碧口地区金铜矿成矿地质背景、矿床地质特征为基础,结合典型矿床剖析,重点从矿床成因、矿床类型、矿床规模、赋矿层位、围岩性质和矿石特征等方面,总结了矿床的... 详细信息

碧口地区地处摩天岭地块,是中国重要的金铜成矿远景区、矿集区。笔者以甘肃碧口地区金铜矿成矿地质背景、矿床地质特征为基础,结合典型矿床剖析,重点从矿床成因、矿床类型、矿床规模、赋矿层位、围岩性质和矿石特征等方面,总结了矿床的分布特征及规律,并提出了同类矿床的找矿标志和找矿潜力。

关键词：碧口地区金铜矿地质特征找矿潜力

在线全文

学校读者我要写书评

暂无评论

胎儿水肿综合征孕产妇的护理

护士进修杂志 2009年第12期24卷 1090-1091页

作者：陈艳雯广东省广州市妇婴医院广东广州510180

目的通过对胎儿水肿综合征孕产妇在产科住院阶段的观察处理和护理经验总结,减少出生缺陷,消除孕产妇产后出血及各种并发症。方法对46例孕产妇实施心理护理、技术护理、产程观察及预防产后出血等并发症的处理和分析总结。结果全部患者均... 详细信息

目的通过对胎儿水肿综合征孕产妇在产科住院阶段的观察处理和护理经验总结,减少出生缺陷,消除孕产妇产后出血及各种并发症。方法对46例孕产妇实施心理护理、技术护理、产程观察及预防产后出血等并发症的处理和分析总结。结果全部患者均康复出院,未发生死亡病例及手术切除出血情况。结论胎儿水肿综合征孕产妇分娩时易发生产时胎盘巨大难剥离、产后出血等,做好积极应对及抢救准备等是保障产妇生命的重要举措。

关键词：胎儿水肿护理

Leber Hereditary Optic Neuropathy in a Boy with Fibrous Boney Dysplasia

在线全文

学校读者我要写书评

暂无评论

Eye Science 2013年第1期28卷 48-50页

作者： Yi Du Benli Jiang Kaijun Li yanwen chen Jianfeng He Department of Ophthalmology First Affiliated Hospital of Guangxi Medical University State Key Laboratory of Ophthalmology Zhongshan Ophthalmic CenterSun Yat-sen University Department of Ophthalmology No.1 People's Hospital of Nanning

Purpose:To report a case of Leber hereditary optic neuropathy combined with fibrous boney dysplasia. Methods: Case report. Results:A 16-year-old boy presented with painless vision loss in both eyes. He had a history of a right humerus fracture and right femoral fracture surgery after an uncomplicated *** examination in our clinic, his visual acuity was counting fingers at 20 cm OD and counting fingers at 40 cm *** pupils reacted sluggishly to *** findings on slit-lamp examination and funduscopy after pupillary dilation were all unremarkable. Computed tomography scans demonstrated fibrous dysplasia involving the right frontal, temporal, parietal, and occipital bones but no stenosis of either optic canal. His serum alkaline phosphatase was 522 U/L (reference range: 40-150 U/L). His vision showed no improvement after intravenous methylprednisolone pulse ***,a 11778 mitochondrial DNA mutation was detected. He still had no visual recovery after treatment with oral coenzyme Q10,vitamin B1, and citicoline. Conclusion:Fibrous dysplasia of bone may be associated with Leber hereditary optic neuropathy,possibly due to the fact that it increases local oxygen consumption. (Eye Science 2013; 28:48-50)

关键词：发育不良视神经遗传性纤维病变计算机断层扫描血清碱性磷酸酶线粒体DNA

Mechanism of peripheral basophils decrease in systemic lupus erythematosus

在线全文

学校读者我要写书评

暂无评论

Mechanism of peripheral basophils decrease in systemic lupus...

第九届全国免疫学学术大会

作者： Qingjun Pan Zhenzhen Deng Lu Li yanwen chen Weijing Liu Huafeng Liu 广东医学院附属医院肾病内科

Objective:To investigate the mechanisms of peripheral basophils decreasing in SLE patients and further clarify the role of basophils in the pathogenesis of ***:Fifty SLE patients were selected and divided into active group(SLEDAI score,10,n=27)and inactive group(SLEDAI score<10,n=23).Normal health as control *** Caspase-3 and Fas expression of peripheral basophils were detected by flow *** levels of anti-dsDNA antibody were assayed by enzyme-linked immunosorbent assay(ELISA).Additionally,the migration of basophils in skin lesions of SLE patients was immunohistochemically analyzed using basophilspecific antibody 2D7,and the migration of basophils to lymph nodes of MRL/lpr mice were detected by flow cytometry(CD49bIgE).Results:Expression of Cleaved Caspase-3 in peripheral basophils from SLE group were significantly higher than that of control group,while there were no significant difference between SLE active group and SLE inactive *** with control group,the rates of Fas expression sigificantly increased in patients of active SLE;Expression of Cleaved Caspase-3 in peripheral basophils were negatively correlated with serum levels of anti-dsDNA antibody in active SLE patiens;Basophils were found in skin lesions of SLE patients staining with 2D7 positive which appears most in the superficial dermis and the inflammatory sites and exhibited both discrete cellular localization and diffuses extracellular staining,while it was not seen in ***,the migration of basophils to lymph nodes were found in MRL/lpr mice,but not in C57BL/6 ***:Migration of basophils to lymph nodes or skin lesions and acceleration of basophils apoptosis participated in the decrease of peripheral basophils in SLE patients,but the role of migrating of basophils in SLE need to be further studied.

关键词： Systemic lupus erythematosus Basophil Decrease,Mechanism

Factors affecting the hyperuricemia in IgA nephropathy patients with renal function compensatory phase in western part of Guangdong province

在线全文

cnki会议

学校读者我要写书评

暂无评论

Factors affecting the hyperuricemia in IgA nephropathy patie...

第十届全国免疫学学术大会

作者： yanwen chen Qiaofen Lan Huanjin Liao Jie Lu Qiuhua chen Tong Xie Huafeng Liu Qingjun Pan Institute of Nephrology Affiliated Hostiptal of Guangdong Medical College Department of Rheumatology and Immunology Affiliated Hospital of Guangdong Medical College

Objective:To analyze the risk factors of hyperuricemia occurrence in Ig A nephropathy patients with renal function compensatory phase in western part of Guangdong ***:The clinical and pathological data of 328 patients with Ig A nephropathy were selected to investigate the risk factors of ***:The incident of hyperuricemia in Ig A nephropathy was 38.4%.Univariate regression analysis suggested that the risk factors of hyperuricemia in patients with Ig A nephropathy were related to age,serum creatinine,blood urea nitrogen,serum triglyceride,serum total cholesterol,glomerulus sclerosis score,glomerular injury integral,tubular-interstitial injury score,renal small vessel injury score and Lee’s *** regression analysis showed that the independent risk factors for hyperuricemia in patients with Ig A nephropathy were serum triglyceride and renal small vessel injury ***:Among the multiple risk factors of hyperuricemia in Ig A nephropathy with renal function compensatory phase in western part of Guangdong province,the independent risk factors were serum triglyceride and renal small vessel injury *** obtaining therapy strategies with regional characteristics,also provide a reference for other Ig A nephropathy patients with renal function compensatory phase.

关键词： Ig A nephropathy hyperuricemia risk factor

Levels of autophagy in T cells and B cell of patients with systemic lupus erythematosus and its clinical significance

在线全文

cnki会议

学校读者我要写书评

暂无评论

Levels of autophagy in T cells and B cell of patients with s...

第十届全国免疫学学术大会

作者： Qiaofen Lan yanwen chen Huanjin Liao Tong Xie Huafeng Liu Qingjun Pan Department of Rheumatology and Immunology Affiliated Hospital of Guangdong Medical College Institute of Nephrology Affiliated Hostiptal of Guangdong Medical College

Objective:To investigate levels of autophagy in T cells and B cell of patients with systemic lupus erythematosus(SLE)and its clinical ***:68 SLE patients withought treatment within 4 weeks were enrolled in this *** accessed the levels of autophagy in T and B cells of 23 healthy controls and 68 patients before and after treatment by flow cytometry,and analyzed their correlations with serum levels of C3 and anti-ds DNA antibodies,SLEDAI score,et ***:Before treatment,a significantly increased levels of LC3-II was observed in SLE patients than healthy controls,the active group(SLEDAI score≥10)was significantly higher than the stable group(SLEDAI scorers=0.289,P<0.05),and the levels of C3 were positively correlated the levels of LC3-II in B lymphocyte(rs=0.371,P<0.01).After treatment for five days,levels of autophagy in T lymphocytes of SLE patients with good prognosis(SLEDAI score decreased≥4)significantly decreased(P<0.05);Also,three days after treatment,levels of autophagy in B lymphocytes of SLE patients with good prognosis were increased ***:Levels of autophagy in T and B lymphocytes of SLE patients were abnormal compared to healthy controls,and these changes were associated with disease ***,these changes are expected to be as indicators of disease activity and potential therapeutic targets in SLE.

关键词： Systemic lupus erythematous T lymphocytes B lymphocytes Autophagy LC3-II

Role of Basophils in Minimal Change Nephrotic Syndrome

在线全文

cnki会议

学校读者我要写书评

暂无评论

Role of Basophils in Minimal Change Nephrotic Syndrome

第九届全国免疫学学术大会

作者： Qingjun Pan Jing Wu Jingli Tao yanwen chen Weijing Liu Huafeng Liu 广东医学院附属医院肾病内科(肾脏疾病研究所)

A large amount of evidence has implicated minimal change nephrotic syndrome(MCNS)may result from the dysfunction of T cells and B cells,while the precise mechanism are not well *** has been widely known that MCNS is a Th2-dominant glomerular disease resulting from imbalanced Th1/Th2 immune *** the increasing levels ofTh2 cytokines,IL-4 and IL-13,are closely correlated with disease ***,basophils which can affect Th1/Th2balance by enhancing Th2 response and impairing Th1 response then be involved in the development of many diseases,however,whether basophils are vital in the pathogenesis of MCNS,remains *** has been reported that frequent positivity of the human basophil degranulation test in patients with ***,basophils should be analyzed in the pathogenesis of MCNS.

关键词： Minimal change nephrotic syndrome Th1/Th2 Basophils IgE Toll-like receptor