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Activatable and Self-Monitoring Hydrogen Sulfide-Based Molecular Senomorphics for Visualized Regulation of Cellular Senescence

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CCS Chemistry 2023年第12期5卷 2897-2909页

作者： Jili Li Yuqi Xie Jian Wang Linlin Wang Yinghao Xia yanlan liu Weihong Tan Molecular Science and Biomedicine Laboratory(MBL) State Key Laboratory of Chemo/Biosensing and ChemometricsCollege of Chemistry and Chemical EngineeringAptamer Engineering Center of Hunan ProvinceHunan UniversityChangshaHunan 410082 The Cancer Hospital of the University of Chinese Academy of Sciences(Zhejiang Cancer Hospital) Hangzhou Institute of Medicine(HIM)Chinese Academy of SciencesHangzhouZhejiang 310022 Institute of Molecular Medicine(IMM) Renji HospitalShanghai Jiao Tong University School of MedicineCollege of Chemistry and Chemical EngineeringShanghai Jiao Tong UniversityShanghai 200240

Specific regulation of the senescence-associated secretory phenotype(SASP)is vital to block senescence-induced detrimental cellular plasticity.Recently,some chemical compounds called senomorphics have demonstrated such potential,but it remains challenging to achieve site-specific activation and real-time monitoring of the action of senomorphics,posing great obstacles for transformable applications.Here,we report a tailor-made hydrogen sulfide(H_(2)S)donor(Lyso-FH_(2)S-Gal)as a new class of molecule senomorphics for spatially controlled delivery of H_(2)S for visualization of regulation of cellular senescence.It comprises four functional moieties in a single molecular structure,including a lysosome-targeting group for cell recognition,a lysosomal enzyme-cleaved scaffold for site-specific activation,thiocarbamate as the H_(2)S precursor,and a switchable fluorophore for concurrent selfreporting of H_(2)S release and senescence imaging.Lyso-FH_(2)S-Gal exhibited remarkable response selectivity,sustained H_(2)S release,and 141-fold fluorescence enhancement.In cellular models,Lyso-FH_(2) S-Gal preferentially enriched in senescent cells over nonsenescent cells,and alleviated the levels of SASP and reactive oxygen species(ROS)in senescent cells,while remaining inert in nonsenescent cells.More impressively,it efficiently inhibited the SASPmediated crosstalk between senescent cells and surrounding nonsenescent cells,thereby preventing senescence propagation.This work offers a useful molecular tool with the hope for controlled intervention of senescence-related important biological processes.

关键词： cellular senescence hydrogen sulfide donor molecular senomorphics visualized release cellular communications

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Unlocking multiplexing in deep tissue

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Science China Chemistry 2019年第2期62卷 157-158页

作者： Yang Sun Zhengyu Deng yanlan liu Weihong Tan Institute of Molecular Medicine Renji Hospital School of Medicine and College of Chemistry and Chemical EngineeringShanghai Jiao Tong University Molecular Science and Biomedicine Laboratory (MBL) State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical Engineering College of Life Sciences Aptamer Engineering Center of Hunan Province Hunan University Department of Chemistry and Department of Physiology and Functional Genomics Center for Research at the Bio/Nano InterfaceHealth Cancer Center UF Genetics Institute Mc Knight Brain Institute University of Florida

In vivo fluorescence imaging in the second near-infrared (NIR-II)window (1000-1700 nm)has been emerging as a new powerful imaging technique and demonstrated tre- mendous potential in life sciences and biomedical applications,given its advances in reducing photon scattering,light absorption,and autofluorescence [1,2].In particular,extensive efforts have been made to produce various NIR-II probes with different emission wavelengths for multiplexing purposes,as practical applications typically require concurrent detection of multiple analytes.

关键词： Unlocking multiplexing deep tissue

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5种杂豆粉的理化性质及凝胶特性

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食品与机械 2024年第5期40卷 168-172页

作者：朴升虎袁洁瑶徐丽刘艳兰易翠平长沙理工大学食品与生物工程学院湖南长沙410114

目的:探究杂豆作为淀粉凝胶类食品配料的潜力。方法:分析了绿豆、豌豆、白芸豆、赤小豆、鹰嘴豆5种杂豆粉的基本成分、水合特性、糊化特性、凝胶特性等。结果:绿豆粉的吸水性指数(WAI)在90℃和水溶性指数(WSI)在40℃时最高,吸水性较好;... 详细信息

目的:探究杂豆作为淀粉凝胶类食品配料的潜力。方法:分析了绿豆、豌豆、白芸豆、赤小豆、鹰嘴豆5种杂豆粉的基本成分、水合特性、糊化特性、凝胶特性等。结果:绿豆粉的吸水性指数(WAI)在90℃和水溶性指数(WSI)在40℃时最高,吸水性较好;糊化结果中,绿豆粉的回生值最高,表现出良好的凝胶性;凝胶特性结果中,绿豆凝胶的硬度、咀嚼度显著高于其他杂豆粉,凝胶有一定弹性,咀嚼性较强;且绿豆凝胶无气泡、表面均匀、光滑度好,拉伸距离最长。结论:绿豆是5种杂豆中最适于做凝胶类食品配料的原料。

关键词：杂豆理化性质凝胶特性

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镁及镁合金植入物在运动医学中的应用研究进展

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中国修复重建外科杂志 2024年第3期38卷 380-386页

作者：吴雨宽白浪刘妍兰韩潜刘俏男艾义翔徐美光温暖洋单智伟尹战海西安交通大学第一附属医院骨科西安710061 西安交通大学材料科学与工程学院西安710049 西安交通大学陕西省镁基新材料工程研究中心西安710049

目的对镁及镁合金植入物在运动相关损伤修复重建中的应用研究进展进行综述。方法广泛查阅近年来国内外相关研究文献,对镁及镁合金材料特性和其用于不同部位运动损伤修复重建的研究进展进行总结。结果镁与镁合金材料在机械性能、生物... 详细信息

目的对镁及镁合金植入物在运动相关损伤修复重建中的应用研究进展进行综述。方法广泛查阅近年来国内外相关研究文献,对镁及镁合金材料特性和其用于不同部位运动损伤修复重建的研究进展进行总结。结果镁与镁合金材料在机械性能、生物安全性和促腱-骨界面愈合能力方面具有优势,目前有关镁与镁合金植入物修复重建运动相关损伤的临床前研究也取得了较好结果,但是其成功临床转化还需解决机械强度和降解行为问题,合金化和表面处理是可行的解决方案。结论镁及镁合金植入物应用于运动损伤修复重建有望实现临床转化,后续应优化其机械强度和降解行为,开展更大规模的生物安全性测试和开发新型含镁植入物是未来研究方向。

关键词：镁镁合金生物可降解植入物运动医学修复重建

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Author Spotlight

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CCS Chemistry 2023年第12期5卷 2679-2684页

作者： Tang, Junwang (John) Stuparu, Mihaiela C. Tian, Tian Xu, Jingsan liu, yanlan Acad Europe London England Tsinghua Univ Mat Chem & Catalysis Beijing Peoples R China UCL London England Nanyang Technol Univ Sch Chem Chem Engn & Biotechnol Singapore Singapore Wuhan Univ Coll Chem Wuhan Peoples R China Queensland Univ Technol Brisbane Australia Hunan Univ Mol Sci & Biomed Lab MBL State Key Lab Chemo Biosensing & Chemometr Changsha Peoples R China Hunan Univ Coll Chem & Chem Engn Changsha Peoples R China

Junwang(John)Tang(唐军旺).Prof.Junwang(John)Tang obtained his Ph.D.in physical chemistry from the Dalian Institute of Chemical Physics(DICP),Chinese Academy of Sciences,in 2001.He is currently a Member of the Academy of Europe,a Fellow of the European Academy of Sciences,a Fellow of the Royal Society of Chemistry(RSC),and a Fellow of the Institute of Materials,Minerals,and Mining(FIMMM).

关键词： Fellow Dalian currently

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Covalently Crosslinked siRNA Nanogels Hitchhike on Lysosomes for Transport,Reseponsive Release and Gene Silencing

Covalently Crosslinked siRNA Nanogels Hitchhike on Lysosomes...

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第十一届全国化学生物学学术会议

作者： Xiaoyan Han yanlan liu Molecular Science and Biomedicine Laboratory State Key Laboratory of Chemo/Biosensing and ChemometricsCollege of Chemistry and Chemical EngineeringHunan University

Most siRNAs intracellular delivered by various vectors were damaged by the endosome-lysosome pathway following receptor-mediated endocytosis.Effective delivery and release siRNA into cytoplasm remains challenging.Herein,we generated a ’carrier-free-siRNA’ nanogel to delivery siRNA without the requirement of a transfection agent,which was conjugated by NHS ester.1 In this study,annexin A11（ANXA11）,an RNA granule-associated phosphoinositide-binding protein,acts as a molecular tether that can dynamically couple siRNA nanogels with lysosomes.This membraneless siRNA nanogels can actively be transported into cancer cell by lysosomes depending on the intrinsic membrane-binding and phase separating properties of ANXA11.2 The siRNA release is governed by the degradation of ester linkages,which is responsive for intercellular high GSH level.The bioactive of released siRNA is confirmed by silencing of green fluorescent protein–expressing in A549 cells.This strategy of reversing intracellular process of lysosomes on siRNA provides a promising insight in the development of siRNA delivery systems.

关键词： siRNA nanogels ANXA11 endosomal-lysosomal degragation

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Kidney-accumulated Ultra-small Melanin Nanoparticles as multifunctional theranostic nanoplatform for AKI therapy

Kidney-accumulated Ultra-small Melanin Nanoparticles as mult...

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第十一届全国化学生物学学术会议

作者： Xi Yuan yanlan liu Weihong Tan Molecular Science and Biomedicine Laboratory State Key Laboratory of Chemo/Bio-Sensing and ChemometricsCollege of Chemistry and Chemical EngineeringAptamer Engineering Center of Hunan ProvinceHunan University

Oxidative stress imbalance has always been considered as a crucial mechanism going with myriads of clinical diseases. As antioxidant therapy is emerging as an innovative strategy, nanoantioxidants possess unparallel superiorities against natural enzymes in stabilities and diversities. [1] However, multifunctional nanoantioxidants, possessing active targeting without any modification remain elusive. Herein, we develop endogenous ultra-small melanin-based nanoparticles（UMPs） as highly potent antioxidant theranostic platform for acute kidney injury（AKI） therapy. AKI is an inflammation-related, life-threatening renal dysfunction disease, emerging as the most severe and common subtype in inpatients.[2] UMPs preferentially accumulate in damaged renal site with a longer retention time, ameliorating clinical symptoms of nephrotoxicity. Furthermore, UMPs possess unique photoacoustic property, which could non-invasively monitor the biodistribution of UMPs by photoacoustic imaging（PAI）. In vivo results further validate the potent protective effect of UMPs against AKI with negligible side effects, which pave the way for the exploration of endogenous agents for the theranostics of inflammation-related diseases.

关键词： antioxidant endogenous photoacoustic

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The Detection of Phosgene with the Near Infrared Fluorescent Probe

The Detection of Phosgene with the Near Infrared Fluorescent...

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第十一届全国化学生物学学术会议

作者： Dailiang Zhang yanlan liu Weihong Tan Molecular Science and Biomedicine Laboratory State Key Laboratory of Chemo/Bio-Sensing and Chemometrics Hunan University

Phosgene is one of the most toxic substances and this substance has gained infamy for it was a chemical weapon agent during the World War I.1 Phosgene has led to nearly 80% of the poison gas deaths and it threaten the safety of human body. However, the phosgene has contributed to human life as it is extensively used as a chemical intermediate in the industrial. Phosgene’s two faces of a colorless and highly toxic gas but a commonly used intermediate make it a serious threat to public health safety. Although there are many probes which have been design and synthesized for the detection of phosgene, there is no probe has been used with Near Infrared emission. NIR probe’s spectral interference caused by nonspecific absorption or the fluorescence of biological or environmental samples is generally lower than that in the visible range;therefore, the great shift of the spectrum in the NIR region is expected to allow high-sensitivity measurements to be achieved. Here, we design and synthesized a NIR fluorescent probe called 820 NN to detect the phosgene sensitively with little interference from background（Scheme 1）. The 820 NN could sensitively and quickly detect the phosgene specifically and we promise it will have a broad application in real life.

关键词： Phosgene NIR Probe

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谷物中多酚与多糖之间相互作用的研究进展

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中国粮油学报 2022年第4期37卷 187-193页

作者：易翠平刘爽林本平刘艳兰长沙理工大学食品与生物工程学院长沙410114

谷物中多酚与细胞壁多糖相互作用的束缚使得多酚在谷物细胞壁中的释放受到阻碍,因此破坏多酚与多糖的相互作用成为促进多酚释放的有效途径。本文综述了谷物中多酚与细胞壁多糖之间的共价相互作用与非共价相互作用。共价相互作用,如酯键... 详细信息

谷物中多酚与细胞壁多糖相互作用的束缚使得多酚在谷物细胞壁中的释放受到阻碍,因此破坏多酚与多糖的相互作用成为促进多酚释放的有效途径。本文综述了谷物中多酚与细胞壁多糖之间的共价相互作用与非共价相互作用。共价相互作用,如酯键、醚键以及糖苷键;非共价相互作用,如氢键和疏水相互作用。本文还介绍了能够破坏多酚与细胞壁多糖相互作用的多种加工方式,如发芽等非热加工和挤压等热加工方式,以期为提高谷物中多酚的利用提供理论参考。

关键词：谷物多酚多糖相互作用

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纤维蛋白原样蛋白1在透明细胞性肾细胞癌中的表达及其临床意义

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肿瘤研究与临床 2023年第8期35卷 573-577页

作者：刘静刘彦兰刘松李臣刘尧刘炜廊坊市中医医院治未病科廊坊065000 廊坊市中医医院病理科廊坊065000 廊坊市人民医院外科廊坊065000 天津市第三中心医院信息中心天津300000 河北医科大学第四医院病理科石家庄050000 廊坊市中医医院内镜科廊坊065000

目的探讨纤维蛋白原样蛋白1(FGL1)在透明细胞性肾细胞癌(ccRCC)中的表达及其与患者临床病理特征的相关性。方法回顾性分析2015年1月至2018年12月确诊并接受手术治疗的242例ccRCC患者的临床病理资料,收集患者癌组织和癌旁组织(距离癌组... 详细信息

目的探讨纤维蛋白原样蛋白1(FGL1)在透明细胞性肾细胞癌(ccRCC)中的表达及其与患者临床病理特征的相关性。方法回顾性分析2015年1月至2018年12月确诊并接受手术治疗的242例ccRCC患者的临床病理资料,收集患者癌组织和癌旁组织(距离癌组织边缘2 cm)。采用免疫组织化学法检测FGL1蛋白的表达情况,应用反转录-聚合酶链反应(RT-PCR)检测FGL1 mRNA的相对表达量,采用Cox比例风险模型对患者无进展生存(PFS)的影响因素进行单因素及多因素分析。结果癌组织中FGL1蛋白阳性率高于癌旁组织[28.5%(69/242)比2.1%(5/242)],差异有统计学意义(χ^(2)=65.34,P<0.001);癌组织中FGL1 mRNA相对表达量亦高于癌旁组织(1.67±0.12比0.60±0.15),差异有统计学意义(t=25.33,P<0.001)。FGL1的表达与病理分期(r=0.164,P=0.011)、肾血管瘤栓(r=0.130,P=0.043)、区域淋巴结转移(r=0.153,P=0.018)和远处转移(r=0.160,P=0.012)均呈正相关。单因素分析显示,肿瘤长径、区域淋巴结转移、病理分期、远处转移、FGL1表达均为患者PFS的影响因素(均P<0.05);多因素分析显示,FGL1高表达(HR=11.679,95%CI 7.432~15.673,P=0.015)、病理分期Ⅲ~Ⅳ期(HR=13.654,95%CI 8.765~18.761,P=0.013)、远处转移(HR=11.387,95%CI 7.662~14.831,P=0.038)均为患者PFS的独立危险因素。结论FGL1在ccRCC中高表达,且与病理分期、肾血管瘤栓、区域淋巴结转移和远处转移具有相关性,是影响患者预后的独立危险因素。

关键词：癌肾细胞纤维蛋白原样蛋白1 临床病理特征预后

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