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Insights into the genetic covariation between harvest survival and growth rate in olive flounder(Paralichthys olivaceus)under commercial production environment

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Aquaculture and Fisheries 2023年第2期8卷 135-140页

作者： yangzhen li Yuanri Hu Yingming Yang Weiwei Zheng Shandong Key Laboratory of Marine Fisheries Biotechnology and Genetic Breeding Yellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao266071China Laboratory for Marine Fisheries Science and Food Production Processes Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao266237China National Demonstration Center for Experimental Fisheries Science Education Shanghai Ocean UniversityShanghai201306China

In aquaculture,selective breeding for survival till harvest have become an alternative strategy for improving disease resistance and ***,knowledge of genetic parameters of harvest survival,e.g.,heritability and genetic correlations between survival and growth rate traits,is still *** aims of this study were to estimate genetic parameters for harvest survival and growth rate traits under commercial farming conditions in olive flounder(Paralichthys olivaceus).Harvest survival was defined as a binary trait;growth traits were measured as average daily gain(ADG),specific growth rate(SGR),daily growth coefficient(DGC)and body weight(BW).Data from a population of 241 full-sib families(involving 39,904 individuals,four generations)were *** of survival were low but significant,which were 0.15±0.04 and 0.22±0.01 on observed and underlying scale,*** estimates for ADG,SGR and DGC were medium to high,which were 0.33±0.06,0.83±0.07,0.58±0.07,*** the heritability of BW is of low magnitude(0.17±0.08).The genetic correlations between harvest survival and three growth rate traits(i.e.,ADG,SGR and DGC)were very strong(ranging from 0.66 to 0.79),which is an exciting ***,the genetic correlation between harvest survival and BW was much lower(0.17±0.08).These results suggest that selection for harvest survival would consequentially result in concomitant increase of growth rate,and vice *** findings revealed novel insights into the genetic improvement of growth rate and harvest survival through genetic selection in olive flounder.

关键词： Heritability Genetic correlation Survival Growth Olive flounder Paralichthys olivaceus

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Estimationof genetic parameters for juvenile growth performance traits in oliveflounder(Paralichthys olivaceus)

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Aquaculture and Fisheries 2019年第2期4卷 48-52页

作者： yangzhen li Bo Zhang Yingming Yang Songlin Chen Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences(CAFS)Laboratory for Marine Fisheries Science and Food Production ProcessesQingdao National Laboratory for Marine Science and TechnologyQingdao266071China College of Fisheries and life Science Shanghai Ocean UniversityShanghai201306China China Key Laboratory for Sustainable Development of Marine Fisheries Ministry of AgricultureQingdao266071China Tianjin Bohai Sea Fisheries Research Institute Bohai Sea Fisheries Research Center of Chinese Academy of Fishery SciencesTianjin300457China

The aim of this study was to evaluate genetic parameters of growth traits for improving selective breeding in olive flounder(Paralichthys olivaceus).A total of 82 full-sib families from 2 year-classes with complete pedigree information were used to estimate heritabilities and genetic/phenotypic correlations for 4 growth performance traits(body weight BW,total length TL,body height BH and condition factor K)based on uni-trait and multi-trait linear mixed models,*** were analyzed by the restricted maximum likelihood(REML)*** heritabilities of BW,TL and BH were 0.81,0.70 and 0.61,which are relatively high,however,heritability for K is low(0.11).For all growth traits,the common full-sib effects(c^(2))were at a low to moderate level(0.14–0.20);the maternal effects(m^(2))of the traits(except K)were also detected(0.15–0.19).The phenotypic and genetic correlations among BW,TL and BH were highly positive(0.70–0.91),however,the correlations between K and the other 3 traits was low or negligible(−0.22–0.26).According to our results,these traits(except K)could be selected and enhanced *** addition,direct selection based on TL or BH,which is an easily measurable trait,will be more favorable than selection on ***,our results indicated that growth parameters estimated in the juvenile stage could be used as a predictor of growth performance at later growth stages.

关键词： Genetic parameter Growth trait Heritability Genetic correlation Olive flounder Paralichthys olivaceus

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Estimation of heritabilities of disease resistance to Edwardsiella tarda and genetic correlations between resistance and growth traits in Chinese tongue sole (Cynoglossus semilaevis)

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Aquaculture and Fisheries 2020年第6期5卷 289-293页

作者： Ming li Yingming Yang Weiwei Zheng Zhongming li Jiayu Cheng yangzhen li College of Fisheries and life Science Shanghai Ocean UniversityShanghai201306China Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences(CAFS)Laboratory for Marine Fisheries Science and Food Production ProcessesQingdao National Laboratory for Marine Science and TechnologyQingdao266071China Weifang Sanxin Aquatic Technology Development Co. Ltd.Weifang261311China Tangshan Weizhuo Aquaculture Co. Ltd.Tangshan063200China

Chinese tongue sole(Cynoglossus semilaevis)is an important marine flatfish which is widely cultured in coastal areas in *** bacterial diseases such as Edwardsiella tarda imposed serious threats to this species.A potential strategy to prevent this disease is to select resistance *** aim of the present work was to estimate the genetic variation of disease resistance to *** and to evaluate the genetic correlations between resistance traits and growth *** types of models were fitted by using different trait definitions(binary,continuous and categorical).After a 9-day challenge test,the overall survival was 75.4%(ranging from 6.4%to 100%in families),and at test day 6,the overall survival was 50.7%.We set test day 6 and 9 as cut-off point times *** heritabilities of survival traits were ranging from 0.10 to *** higher heritability values were obtained at day 6 than at day 9,regardless of which model or trait definition used(except Trait 2 in liN).The genetic correlations between disease resistance traits and growth traits(*** weight and total length)were low and not significant from zero(−0.12–0.24).There is a substantial re-ranking of families when defined resistance as categorical and continuous traits compared to binary *** results confirm the existence of genetic variation for resistance against *** and weak genetic correlations indicate that joint genetic improvement of *** resistance and growth is scarcely available.

关键词： Heritability Genetic parameter Disease resistance Genetic correlation Edwardsiella tarda Cynoglossus semilaevis

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Transcriptome analysis and candidate gene identification reveals insights into the molecular mechanisms of hypermelanosis in Chinese tongue sole(Cynoglossus semilaevis)

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Aquaculture and Fisheries 2022年第6期7卷 601-609页

作者： yangzhen li Peng Cheng Ming li Yuanri Hu Zhongkai Cui Chuantao Zhang Songlin Chen Key Laboratory for Sustainable Development of Marine Fisheries Ministry of AgricultureYellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao266071China Laboratory for Marine Fisheries Science and Food Production Processes Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao266237China National Demonstration Center for Experimental Fisheries Science Education Shanghai Ocean UniversityShanghai201306China

Blind-side hypermelanosis has emerged as a major concern in flatfish aquaculture worldwide,including tongue sole(Cynoglossus semilaevis)in *** causative gene and the molecular basis are still *** this study,comparative transcriptome analyses were performed using different skin tissues of tongue sole:ocular-side normal(pigmented)skin,blind-side normal(non-pigmented)skin and blind-side hypermelanotic(pigmented)***,60 key hypermelanosis-related genes were mined,providing potential candidate gene resources involved in blind-side *** genes were selected based on the log2(FoldChange)and false discovery rate(FDR)values(with corresponding P-Valueslidation of 16 differentially expressed genes(DEGs)showed that the data of qRT-PCR were consistent with those of *** analyses revealed that the selected DEGs were significantly overrepresented in several pigment metabolic processes and in the melanogenesis *** results may imply that blind-side hypermelanosis is a pattern of environmental regulation of gene expression and adaptation in ***brane transport proteins(such as OCA2 and SLC45A2)may serve as a“switch”for melanogenesis in tongue ***,this study provided novel insights into the molecular mechanism of hypermelanosis in flatfish species and will facilitate future selective breeding of tongue sole for this market-favoured trait in aquaculture.

关键词： Cynoglossus semilaevis Hypermelanosis Transcriptome Flatfish

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Effects of dietary supplementation of Lactobacillus plantarum and Bacillus subtilis on growth performance,survival,immune response,antioxidant capacity and digestive enzyme activity in olive flounder(Paralichthys olivaceus)

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Aquaculture and Fisheries 2021年第3期6卷 283-288页

作者： yangzhen li Yingming Yang limin Song Jialin Wang Yuanri Hu Qian Yang Peng Cheng Jianhai li Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences(CAFS)Laboratory for Marine Fisheries Science and Food Production ProcessesPilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao266071China Tianjin Fisheries Research Institute Tianjin300221China Sangtong Bio-engineering(Weifang)CO.Ltd Weifang261210China

In present study,a 10-month feeding trial was carried out to investigate effects of dietary Lactobacillus plantarum and Bacillus subtilis supplementation singly on growth performance,innate immune response,antioxidant capacity and digestive enzyme activity of olive flounder under a commercial production *** experimental groups fed basal diet with supplementation of ***(5.0×107 CFU/g diet)and ***(1.0×108 CFU/g diet)respectively,and control group fed with basal diet without probiotics *** harvest,serum immune parameters,liver antioxidant indices and digestive enzyme activities in liver,stomach and intestines were *** fed with *** supplemented diet showed the highest growth rate which was significantly higher than *** supplemented group(Plive flounder aquaculture,favorable effects of compounded supplementation of *** and *** are expected.

关键词： Antioxidant capacity Digestive enzyme Growth performance Immune response Olive flounder Probiotics

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iTRAQ-based analysis of 17β-estradiol induced proteome in Chinese tongue sole Cynoglossus semilaevis

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Journal of Oceanology and limnology 2019年第5期37卷 1659-1668页

作者： ZHU Ying li yangzhen li Hailong WANG Lei ZHANG Ning liU Yang MENG liang XU Xiwen DONG Zhongdian WEI Min GUO Hua CUI Zhongkai li Xihong SHAO Changwei XU Wenteng Key Laboratory of Sustainable Development of Marine Fisheries Ministry of AgricultureYellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071China Laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and TechnologyQingdao 266237China Research Institute of Metabolic Disease Qingdao UniversityQingdao 266003China

The phenomenon of sex dimorphism prevails among many fi sh species. It has attracted the general research interest due to its close relationship to fi sh growth and thus aquaculture productivity. In Chinese tongue sole ( Cynoglossus semilaevis ), 17β-estradiol (E2) can be used reportedly to induce the feminization of fi sh, although the detailed regulatory network remained elusive. We treated the tongue sole fry before sex diff erentiation with E2 (10 and 30 μg/L) to identify potential targets of E2 in Chinese tongue sole. The E2 treatment indeed induced genetic male fi sh sex-reversal to phenotypic female. Using an iTRAQ-based comparative proteomic analysis, 409 proteins that diff erentially expressed after E2 induction were identifi ed, including 259 up-regulated and 150 down-regulated proteins. Furthermore, 19 diff erentially expressed proteins identifi ed in the comparative proteomic analysis were selected to assess their transcription and eight of them exhibited the same tendency. Functions of the eight proteins included mainly nucleotide and protein binding. Interestingly, a far upstream element-binding protein 3-like isoform exhibited the signifi cant upregulation both at transcription and translation levels after E2 treatment. This work identifi ed a set of candidate proteins for E2 response and deepened our understanding of E2 regulatory mechanism.

关键词： Cynoglossus semilaevis 17β-estradiol sex reversal proteome iTRAQ analysis

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物流服务供应链可靠性影响因素识别研究

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物流技术 2013年第4期32卷 207-210页

作者：李阳珍西南交通大学交通运输与物流学院四川成都610041 西南民族大学管理学院四川成都610041

物流服务供应链可靠性是物流客户感知物流服务质量的核心,而可靠性影响因素可能导致物流服务故障,影响到系统的可靠性。因此,对系统可靠性影响因素识别是可靠性管理的基础。在分析物流服务供应链的运作基础上,运用故障树分析法来对系统... 详细信息

物流服务供应链可靠性是物流客户感知物流服务质量的核心,而可靠性影响因素可能导致物流服务故障,影响到系统的可靠性。因此,对系统可靠性影响因素识别是可靠性管理的基础。在分析物流服务供应链的运作基础上,运用故障树分析法来对系统可靠性影响因素进行识别分析,为系统的可靠性管理提供了决策依据。

关键词：物流服务供应链可靠性物流服务故障故障树最小割集

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Ba(1-3x/2)Lax(Mg(1/3)Ta(2/3))O3陶瓷的微波介电性能(英文)

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硅酸盐学报 2008年第6期36卷 739-742,747页

作者：董云飞李杨珍袁亮亮卞建江上海大学材料科学与工程学院上海200072

采用固相合成法制备了Ba1–3x/2Lax(Mg1/3Ta2/3)O3陶瓷,研究了La掺杂对钽镁酸钡的结构和微波介电性能的影响。结果表明:A位取代能改进其烧结性能。在x≤0.02时,烧结样品为单相的钙钛矿结构,B位离子1:2有序;当x>0.02时出现第二相Ba0.5TaO... 详细信息

采用固相合成法制备了Ba1–3x/2Lax(Mg1/3Ta2/3)O3陶瓷,研究了La掺杂对钽镁酸钡的结构和微波介电性能的影响。结果表明:A位取代能改进其烧结性能。在x≤0.02时,烧结样品为单相的钙钛矿结构,B位离子1:2有序;当x>0.02时出现第二相Ba0.5TaO3。B位离子有序度随着x的增大先增加后减小,在x=0.04时出现最大值。x≤0.02时介电常数变化较小,而后其值逐渐增大。品质因数与谐振频率的乘积(Q×f)值随着x的增大先增大后减小,在x=0.02时取得最大值;谐振频率温度系数(τf)值随着x增大而增大。

关键词：钽镁酸钡微波介电性能改性

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基于Markov过程的物流服务供应链可靠性分析

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重庆交通大学学报（自然科学版） 2012年第4期31卷 895-899页

作者：李阳珍张喜征西南交通大学交通运输与物流学院四川成都610031 西南民族大学管理学院四川成都610041 湖南大学工商管理学院湖南长沙410082

分析了物流供应能力与物流需求的关系,建立了物流服务供应链的二级结构图;提出了一种基于Markov过程的系统可靠性分析模型,对Markov状态转移方程进行求解,从而得到系统处于各状态的稳态概率。研究表明:物流服务供应链的可靠性受其结构... 详细信息

分析了物流供应能力与物流需求的关系,建立了物流服务供应链的二级结构图;提出了一种基于Markov过程的系统可靠性分析模型,对Markov状态转移方程进行求解,从而得到系统处于各状态的稳态概率。研究表明:物流服务供应链的可靠性受其结构的影响,串并联结构可靠性优于串联结构可靠性;各节点企业故障率与修复率也会影响物流服务的可靠性。

关键词：物流服务供应链物流能力可靠性马尔可夫过程

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固态发酵黑曲霉产单宁酶发酵条件研究

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食品与发酵工业 2008年第6期34卷 54-56,61页

作者：保玉心邱树毅李秧针黄永光贵州省发酵工程与生物制药重点实验室

经紫外线诱变黑曲霉菌株筛选出1株高产单宁酶的黑曲霉B0201,利用五倍子为诱导物,固态发酵该菌株得到的单宁酶活力有较大提高。单因素优化试验表明,五倍子用量为8%,初始水分含量为50%,初始pH6.0,温度30℃为优化产酶条件。在优化的条件下... 详细信息

经紫外线诱变黑曲霉菌株筛选出1株高产单宁酶的黑曲霉B0201,利用五倍子为诱导物,固态发酵该菌株得到的单宁酶活力有较大提高。单因素优化试验表明,五倍子用量为8%,初始水分含量为50%,初始pH6.0,温度30℃为优化产酶条件。在优化的条件下,培养96h后产单宁酶酶活力达到了58.2 U/g(干基)。因此,黑曲霉固态发酵产单宁酶具有很大的研究意义及广阔的应用前景。

关键词：黑曲霉单宁酶固态发酵

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