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Phenotype-Directed DNA Nanomachine for in Situ Analysis of Stem Cell-like Subpopulation in Breast Cancer

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CCS Chemistry 2024年第4期6卷 1034-1046页

作者： ya Cao Huiru Mao Lingjun sha Qi Liu Bing Han Jing Zhao Genxi Li Center for Molecular Recognition and Biosensing Shanghai Engineering Research Center of Organ RepairSchool of Life SciencesShanghai UniversityShanghai 200444 State Key Laboratory of Analytical Chemistry for Life Science School of Life SciencesNanjing UniversityNanjing 210023

The cancer stem cell hypothesis provides a basis for prediction of the recurrence and risk of metastasis in breast ***,the unique expression pattern of stemness markers and the presence of nonstem-like cancer cells with varied phenotypes have brought great challenges to the characterization of breast cancer stem *** address these challenges,a phenotype-directed DNA nanomachine has been designed for high-accuracy labeling and in situ analysis of the stem cell-like subpopulation in breast *** key for the design is to use cell surfaceanchored inputs to activate the nanomachine,which undergoes different branch migration pathways such that the signal strand can only be brought onto the cancer cells having the stem cell-like *** sensitive determination and single-step isolation of the stem cell-like subpopulation were achieved by incorporating functional groups into the signal strand such that the nanomachine was successfully applied in a tumor-bearing mouse ***,the approach provides for a substantial improvement in capability for the analysis of the breast cancer stem cell-like subpopulation,and it is expected that the new approach will advance the use of DNA nanomachines in cancer-related studies.

关键词： breast cancer DNA nanomachine stem cell-like subpopulation electrochemical determination single-step isolation phenotypic analysis

Molecular mechanism of hypoxia and alpha-ketoglutaric acid on collagen expression in scleral fibroblasts

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International Journal of Ophthalmology(English edition) 2024年第10期17卷 1780-1790页

作者： Yun Sun Zhuo-Zheng Li Jing yang ya-Ru sha Xin-Yu Hou Hong Fu Jia-Yin Li Shu-Chang Bai Yong-Fang Xie Guo-Hui Wang School of Life Science and Technology Shandong Second Medical UniversityWeifang 261053Shandong ProvinceChina

AIM:To investigate the molecular mechanisms underlying the influence of hypoxia and alpha-ketoglutaric acid(α-KG)on scleral collagen ***:Meta-analysis and clinical statistics were used to prove the changes in choroidal thickness(ChT)during *** establishment of a hypoxic myopia model(HYP)for rabbit scleral fibroblasts through hypoxic culture and the effects of hypoxia andα-KG on collagen expression were demonstrated by Sirius red *** analysis was used to verify the genes and pathways that hypoxia andα-KG affect collagen ***,real-time quantitative reverse transcription polymerase chain reaction(RT-qPCR)was used for reverse ***:Meta-analysis results aligned with clinical statistics,revealing a thinning of ChT,leading to scleral *** red staining indicated lower collagen expression in the HYP group and higher collagen expression in the HYP+α-KG group,showed that hypoxia reduced collagen expression in scleral fibroblasts,whileα-KG can elevated collagen expression under HYP *** analysis unveiled the related genes and signaling pathways of hypoxia andα-KG affect scleral collagen expression and the results were verified by ***:The potential molecular mechanisms through which hypoxia andα-KG influencing myopia is unraveled and three novel genes TLCD4,TBC1D4,and EPHX3 are *** findings provide a new perspective on the prevention and treatment of myopia via regulating collagen expression.

关键词： sclera scleral collagen expression hypoxia alpha-ketoglutaric acid myopia

Berberine Inhibits Ferroptosis and Stabilizes Atherosclerotic Plaque through NRF2/SLC7A11/GPX4 Pathway

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Chinese Journal of Integrative Medicine 2024年第10期30卷 906-916页

作者： WANG Ting-ting YU Li-li ZHENG Jun-meng HAN Xin-yi JIN Bo-yuan HUA Cheng-jun CHEN Yu-shan shaNG sha-sha LIANG ya-zhou WANG Jian-ru Heart Center the First Affiliated Hospital of Henan University of Chinese MedicineZhengzhou450000China

Objective:To investigate potential mechanisms of anti-atherosclerosis by berberine(BBR)using ApoE-/-***:Eight 8-week-old C57BL/6J mice were used as a blank control group(normal),and 568-week-old AopE-/-mice were fed a high-fat diet for 12 weeks,according to a completely random method,and were divided into the model group,BBR low-dose group(50 mg/kg,BBRL),BBR medium-dose group(100 mg/kg,BBRM),BBR high-dose group(150 mg/kg,BBRH),BBR+nuclear factor erythroid 2-related factor 2(NRF2)inhibitor group(100 mg/kg BBR+30 mg/kg ML385,BBRM+ML385),NRF2 inhibitor group(30 mg/kg,ML385),and positive control group(2.5 mg/kg,atorvastatin),8 in each *** 4 weeks of intragastric administration,samples were collected and serum,aorta,heart and liver tissues were *** kits were used to detect serum lipid content and the expression levels of malondialdehyde(MDA)and superoxide dismutase(SOD)in all experimental *** pathological changes of atherosclerosis(AS)were observed by aorta gross Oil Red O,aortic sinus hematoxylin-eosin(HE)and Masson *** lipopathy was observed in mice by HE *** morphology of mitochondria in aorta cells was observed under transmission electron *** cytometry was used to detect reactive oxygen species(ROS)expression in aorta of mice in each *** content of ferrous ion Fe^(2+)in serum of mice was detected by biochemical *** mRNA and protein relative expression levels of NRF2,glutathione peroxidase 4(GPX4)and recombinant solute carrier family 7 member 11(SLC7A11)were detected by quantitative real time polymerase chain reaction(RT-q PCR)and Western blot,***:BBRM and BBRH groups delayed the progression of AS and reduced the plaque area(P<0.01).The characteristic morphological changes of ferroptosis were rarely observed in BBR-treated AS mice,and the content of Fe^(2+)in BBR group was significantly lower than that in the model group(P<0.01).BBR decreased ROS and MDA levels in mouse

关键词： berberine ApoE−/− ferroptosis atherosclerosis oxidative stress NRF2/SLC7A11/GPX4 pathway

顾及土壤热传导-对流效应的InSAR冻土活动层厚度估计

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地球物理学报 2024年第7期67卷 2568-2581页

作者：杨沙王琪洁李佳晨张亚中南大学地球科学与信息物理学院长沙410083 中南大学有色金属成矿预测与地质环境监测教育部重点实验室长沙410083

冻土活动层厚度(Active Layer Thickness,ALT)的变化是反映青藏高原多年冻土发育情况及其状态的一个重要指标,监测活动层厚度变化对寒区景观稳定发展、碳循环等方面具有非常重要的意义.由于合成孔径雷达干涉测量(Interferometric Synthe... 详细信息

冻土活动层厚度(Active Layer Thickness,ALT)的变化是反映青藏高原多年冻土发育情况及其状态的一个重要指标,监测活动层厚度变化对寒区景观稳定发展、碳循环等方面具有非常重要的意义.由于合成孔径雷达干涉测量(Interferometric Synthetic Aperture Radar,InSAR)技术具有大范围、高精度、高时空分辨率等优势,近年来逐渐被用于反演活动层厚度.已有研究中基于InSAR和土壤一维热传导模型的活动层厚度估计方法,没有充分考虑到冻土中土壤水分对流引起的热量传递.因此本文提出了基于InSAR时序形变及土壤热传导-对流模型的活动层厚度估计方法,利用土壤热传导-对流模型建立InSAR探测的最大融沉形变与最高地温之间的滞后时间与活动层厚度之间的相关关系,实现了由滞后时间直接推算大范围、高分辨率的冻土活动层厚度.本文以青藏高原五道梁多年冻土区为例,利用116景Sentinel-1影像图作为实验数据,估计了该地区2017—2020年的平均活动层厚度.结果表明,活动层厚度值范围为0~7.0 m,平均活动层厚度为3.06 m,与已有研究中相近时间段相关成果及观测数据结果一致.本文方法兼顾冻土水热过程,证明了土壤热传导-对流模型在冻土活动层厚度反演中具有良好的应用前景,可用于青藏高原其他偏远地区的高空间分辨率活动层厚度反演.

关键词：冻土土壤热传导-对流模型青藏高原活动层厚度 InSAR

The application of metal-organic frameworks and their derivatives for lithium-ion capacitors

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新型炭材料（中英文） 2024年第5期39卷 872-895页

作者： ZHAO sha-sha ZHANG Xiong LI Chen AN ya-bin HU Tao WANG Kai SUN Xian-zhong MA yan-wei Key Laboratory of High Density Electromagnetic Power and Systems(Chinese Academy of Sciences) Institute of Electrical EngineeringChinese Academy of SciencesBeijing 100190China University of Chinese Academy of Sciences Beijing 100049China shandong Key Laboratory of Advanced Electromagnetic Conversion Technology Institute of Electrical Engineering and Advanced Electromagnetic Drive TechnologyQilu ZhongkeJinan 250013China

There is an urgent need for lithium-ion capacitors(LICs)that have both high energy and high power densities to meet the continuously growing energy storage *** effectively balance the high energy density of traditional rechargeable batteries with the superior power density and long life of supercapacitors(SCs).Nevertheless,the development of LICs is still hampered by limited kinetic processes and capacity mismatch between the cathode and ***-organic frameworks(MOFs)and their derivatives have received significant attention because of their extensive specific surface area,different pore structures and topologies,and customizable functional sites,making them compelling candidate materials for achieving high-performance ***-derived carbons,known for their exceptional electronic conductivity and large surface area,provide improved charge storage and rapid ion ***-derived transition metal oxides contribute to high specific capacities and improved electrochemical ***,MOF-derived metal compounds/carbons provide combined effects that increase both the capacitive and Faradaic reactions,leading to a superior overall *** review begins with an overview of the fundamental principles of LICs,followed by an exploration of synthesis strategies and ligand selection for MOF-based composite *** then analyzes the advantages of original MOFs and their derived materials,such as carbon materials and metal compounds,in enhancing LIC ***,the review discusses the major challenges faced by MOFs and their derivatives in LIC applications and offers future research directions and recommendations.

关键词： Lithium-ion capacitors MOFs Transition metal oxide Energy density Power density

Deep electrical structures of Qinzhou-Fangcheng Junction Zone in Guangxi and seismogenic environment of the 1936 Lingshan M6^(3/4)earthquake

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Science China Earth Sciences 2024年第2期67卷 584-603页

作者： Bin ZHOU Chunheng yaN yan ZHAN Xiangyu SUN sha LI Xiang WEN yajun MO Yongdong YUAN Yuan YUAN Meili HUANG Earthquake Agency of Guangxi Zhuang Autonomous Region Nanning530022China State Key Laboratory of Earthquake Dynamics Institute of GeologyChina Earthquake AdministrationBeijing100029China Guangxi Geophysical Survey Institute Liuzhou545005China

The tectonic position of the southwest section of the Qinzhou Bay-Hangzhou Bay Tectonic Junction Zone(QHTJZ)can be determined by examining the Qinzhou-Fangcheng Junction Zone(QFJZ)in *** zone is significant because it was the location of the largest earthquake ever recorded in the inland region of South China,specifically the 1936 Lingshan M6^(3/4)earthquake in ***,this region serves as an optimal location for researching the origins of intraplate earthquakes in South *** study presents a display of a broadband magnetotelluric(MT)prospecting profile that traverses the Guangxi QFJZ and the Lingshan earthquake zone,extending from the northwest(NW)to the southeast(SE).A resistivity structure model was generated using three-dimensional(3D)inversion technology along the *** main faults in QFJZ were analyzed in terms of their deep extension forms and tectonic *** analysis was performed by integrating the results obtained from geology,gravity,wave velocity ratio,Global Position System(GPS),and geothermal *** results showed that(1)the Dongzhong-Xiaodong fault(DXf),the eastern Fangcheng-Lingshan fault(FLf2),and the eastern Hepu-Beiliu fault(HBf2)were all trans-crustal deep faults,and crust-mantle ductile shear zones developed in the deep *** electrical boundary zones,DXf and HBf2,were *** inclined towards the northwest,while HBf2 inclined towards the *** FangchengLingshan fault(FLf)exhibits a tectonic style resembling a“flower”shape in the upper *** the deeper section,it is characterized by an electrical boundary zone that gradually slopes towards the southeast direction.(2)The Hunan-Guangxi Passive Continental Margin(HGPCM)on the NW side of DXf had a stratified resistivity structure and relatively stable Bouguer gravity anomalies,which conformed to the quasi-craton tectonic attribute of the local failure at the southeastern margin of the yangtze Block(YB).The southeastern side of this bloc

关键词： South China Block Qinzhou-Fangcheng Junction Zone Magnetotelluric 1936 Lingshan M6^(3/4)earthquake Deep seismogenic environment

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面向鲁棒图结构防御的过参数化图神经网络

软件学报 2024年第8期35卷 3878-3896页

作者：初旭马辛宇林阳王鑫王亚沙朱文武梅宏清华大学计算机科学与技术系北京100084 北京大学计算机学院北京100871 高可信软件技术教育部重点实验室(北京大学) 北京100871 清华大学北京信息科学与技术国家研究中心北京102206 北京大学软件工程国家工程研究中心北京100871

图数据在现实应用中普遍存在,图神经网络(GNN)被广泛应用于分析图数据,然而GNN的性能会被图结构上的对抗攻击剧烈影响.应对图结构上的对抗攻击,现有的防御方法一般基于图内聚先验进行低秩图结构重构.但是现有的图结构对抗防御方法无法... 详细信息

图数据在现实应用中普遍存在,图神经网络(GNN)被广泛应用于分析图数据,然而GNN的性能会被图结构上的对抗攻击剧烈影响.应对图结构上的对抗攻击,现有的防御方法一般基于图内聚先验进行低秩图结构重构.但是现有的图结构对抗防御方法无法自适应秩真值进行低秩图结构重构,同时低秩图结构与下游任务语义存在错配.为了解决以上问题,基于过参数化的隐式正则效应提出过参数化图神经网络(OPGNN)方法,并形式化证明所提方法可以自适应求解低秩图结构,同时证明节点深层表征上的过参数化残差链接可以有效解决语义错配.在真实数据集上的实验结果表明,OPGNN方法相对于现有基线方法具有更好的鲁棒性,同时,OPGNN方法框架在不同的图神经网络骨干上如GCN、APPNP和GPRGNN上显著有效.

关键词：图节点半监督分类图结构对抗防御过参数化隐式正则化图神经网络

Wnt3a减轻黑素细胞iMC65氧化应激损伤

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基础医学与临床 2017年第10期37卷 1389-1394页

作者：郑妍杨珂李娅莎董世访周丽娜重庆医科大学附属儿童医院儿科研究所儿童发育疾病研究教育部重点实验室儿童发育重大疾病国家国际科技合作基地重庆市干细胞治疗工程技术研究中心重庆400014

目的探讨Wnt3a对氧化应激损伤的i MC65黑素细胞的保护作用及机制。方法将黑素细胞分成对照组,Wnt3a组,H2O2处理组,Wnt3a干预组,750μmol/L的H2O2作用模拟黑素细胞的氧化应激损伤。MTT实验检测细胞活性,流式细胞术检测细胞凋亡率和细胞... 详细信息

目的探讨Wnt3a对氧化应激损伤的i MC65黑素细胞的保护作用及机制。方法将黑素细胞分成对照组,Wnt3a组,H2O2处理组,Wnt3a干预组,750μmol/L的H2O2作用模拟黑素细胞的氧化应激损伤。MTT实验检测细胞活性,流式细胞术检测细胞凋亡率和细胞产生活性氧(ROS),荧光素酶报告基因检测Nrf2/ARE通路的激活,Western blot检测Nrf2/ARE通路的相关蛋白表达。结果与对照组相比,H2O2处理组的细胞活性明显下降(P<0.01),凋亡比率明显上升(P<0.01),ROS的产生明显增加(P<0.01)。而Wnt3a干预组能显著缓解H2O2处理组细胞活性的降低(P<0.05)、降低凋亡比率(P<0.05),减少ROS的产生(P<0.05)。Wnt3a也能上调Nrf2和HO-1蛋白水平的表达。结论 Wnt3a可以保护氧化应激状态下的黑素细胞,其机制可能与激活Nrf2/ARE有关。

关键词： Wnt3a 黑素细胞 Nrf2 氧化应激

新型Cr-Mo-V型热作模具钢高温流变应力模型

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塑性工程学报 2024年第5期31卷 124-131页

作者：元亚莎石如星王文焱徐恩献王九花张玉栋元莎河南科技大学材料科学与工程学院河南洛阳471023 洛阳中重铸锻有限责任公司河南洛阳471039 中信重工机械股份有限公司河南洛阳471039 中国机械总院集团北京机电研究所有限公司北京100083

通过热压缩实验,获得实验钢在变形温度为950~1200℃、应变速率为0.001~5 s^(-1)和最大真应变为0.916条件下的真应力-真应变曲线。结果表明:在本实验选取的变形条件下,新型Cr-Mo-V钢呈现出典型的动态再结晶特征,变形温度高于1050℃且应... 详细信息

通过热压缩实验,获得实验钢在变形温度为950~1200℃、应变速率为0.001~5 s^(-1)和最大真应变为0.916条件下的真应力-真应变曲线。结果表明:在本实验选取的变形条件下,新型Cr-Mo-V钢呈现出典型的动态再结晶特征,变形温度高于1050℃且应变速率低于0.1 s^(-1)时,动态再结晶特征更明显;同一变形温度下,峰值应力和峰值应变随应变速率的提高呈增大趋势;同一应变速率下,峰值应力和稳态应力随变形温度的升高逐渐降低。采用多元线性回归法获得与峰值应力对应的Arrhenius方程模型参数,基于此建立了实验钢的高温流变应力本构模型。利用创建的不同应变下本构方程材料常数的八次多项式对实验钢的流变应力进行计算,应变补偿相关系数R和平均绝对相对误差e AARE分别为0.98902和3.21%,计算数据与实验数据吻合良好。

关键词：热作模具钢热压缩流变应力本构方程八次多项式