Molecular mechanism of hypoxia and alpha-ketoglutaric acid on collagen expression in scleral fibroblasts
作者机构：School of Life Science and TechnologyShandong Second Medical UniversityWeifang 261053Shandong ProvinceChina
出 版 物：《International Journal of Ophthalmology(English edition)》 (国际眼科杂志（英文版）)
年 卷 期：2024年第17卷第10期
页 面：1780-1790页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：Supported by the Natural Science Foundation of Shandong Province,China(No.ZR2023MA069) the Medical and Health Technology Development Project of Shandong Province,China(No.202202050602) College Students’Innovation and Entrepreneurship Training Program(No.S202410438017) the Graduate Student Research Grant from Shandong Second Medical University
主 题：sclera scleral collagen expression hypoxia alpha-ketoglutaric acid myopia
摘 要：AIM:To investigate the molecular mechanisms underlying the influence of hypoxia and alpha-ketoglutaric acid(α-KG)on scleral collagen ***:Meta-analysis and clinical statistics were used to prove the changes in choroidal thickness(ChT)during *** establishment of a hypoxic myopia model(HYP)for rabbit scleral fibroblasts through hypoxic culture and the effects of hypoxia andα-KG on collagen expression were demonstrated by Sirius red *** analysis was used to verify the genes and pathways that hypoxia andα-KG affect collagen ***,real-time quantitative reverse transcription polymerase chain reaction(RT-qPCR)was used for reverse ***:Meta-analysis results aligned with clinical statistics,revealing a thinning of ChT,leading to scleral *** red staining indicated lower collagen expression in the HYP group and higher collagen expression in the HYP+α-KG group,showed that hypoxia reduced collagen expression in scleral fibroblasts,whileα-KG can elevated collagen expression under HYP *** analysis unveiled the related genes and signaling pathways of hypoxia andα-KG affect scleral collagen expression and the results were verified by ***:The potential molecular mechanisms through which hypoxia andα-KG influencing myopia is unraveled and three novel genes TLCD4,TBC1D4,and EPHX3 are *** findings provide a new perspective on the prevention and treatment of myopia via regulating collagen expression.
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