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AN AMPEROMETRIC BIOSENSOR BASED ON COVALENT IMMOBILIZATION OF ASCORBATE OXIDASE ON BIOCOMPATIABLE AND LOW-TOXIC POLY(THIOPHENE-3-ACETIC ACID) MATRIX

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Chinese Journal of Polymer Science 2012年第5期30卷 705-718页

作者： Dong Li yang-ping wen 徐景坤贺浩华 Ming Liu Jiangxi Key Laboratory of Organic Chemistry Jiangxi Science and Technology Normal University Key Laboratory of Crop Physiology Ecology and Genetic BreedingMinistry of EducationKey Laboratory of PhysiologyEcology and Cultivation of Double Cropping RiceMinistry of AgricultureJiangxi Agricultural University

The biocompatiable and low-toxic poly（thiophene-3-aeetic acid）（PTAA） matrix was successfully electrosynthesized in ionic liquid 1-butyl-3-metbylimidazolium hexafluorophosphate （BmimPF6） in comparison with the electrosynthesis of PTAA matrix in acetonitrile （ACN）. Ascorbate oxidase （AO） was used as a model for the development and application of biosensor. Vitamin C （VC） biosensors were facilely fabricated by the covalent immobilization of AO molecules on PTAA matrices electrosynthesized in ACN containing tetrabutylammonium tetrafluoroborate and BmimPF6, respectively. Electrochemical impedance spectroscopy, scanning electron microscopy and FTIR spectroscopy indicated that AO molecules were covalently immobilized on PTAA matrices. Parameters of the as-obtained biosensors such as working potential, pH and temperature have been optimized. The amperometric biosensor based on PTAA matrix electrosynthesized in BmimPF6 exhibited wider linear range, lower detection limit, higher sensitivity and bioaffinity, and better operational and storage stability than that electrosynthesized in ACN under optimal conditions. The as-obtained biosensor based on PTAA matrix electrosynthesized in BmimPF6 was employed for the detection of VC content in comme：rcial juices, and the result was close to the data given by manufacturers. Excellent results indicate that the PTAA matrix electrosynthesized in ionic liquid is a promising platform for the covalent immobilization of biologically-active species and the development of biosensors.

关键词： Poly(thiophene-3-acetic acid) Ionic liquids Ascorbate oxidase Vitamin C Biosensor.

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POLY(THIOPHENE-3-ACETIC ACID)-PALLADIUM NANOPARTICLE COMPOSITE MODIFIED ELECTRODES FOR SUPERSENSITIVE DETERMINATION OF HYDRAZINE

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Chinese Journal of Polymer Science 2013年第3期31卷 419-426页

作者： Ou Zhang Hong-mei Yu Li-min Lu yang-ping wen 段学民徐景坤 School of Chemical Engineering University of Science and Technology Liaoning School of Pharmacy Jiangxi Science & Technology Normal University

A promising electrochemical sensor was fabricated by electrodeposition of Pd nanoparticles （PdNPs） on poly（thiophene-3-acetic acid）（PTAA）-modified glassy carbon electrode （GCE）, forming a PdNPs/PTAA composites-modified GCE （PdNPs/PTAA/GCE）. Scanning electron microscope （SEM） and electrochemical techniques were used for the characterization of these composites. It was found that the PdNPs/PTAA layer was very uniform. ]Electrochemical experiments showed that this proposed PdNPs/PTAA composites-modified electrode exhibited excellent electrocatalytic activity towards the oxidation of hydrazine. Under the optimum conditions, the proposed sensor can be applied to the quantification of hydrazine with a wide linear range from 8.0 × 10-9 mol/L to 1.0 × 10-5 mol/L with a low detection limit of 2.67 × 10-9 mol/L. The experiment results also showed that the sensor exhibited good reproducibility and long-term stability, as well as high selectivity with no interference from other potential competing species.

关键词： Sensor Hydrazine Nanocomposites Pd nanoparticles Conducting polymer.

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Electrochemical determination of quercetin by self-assembled platinum nanoparticles/poly(hydroxymethylated-3,4-ethylenedioxylthiophene) nanocomposite modified glassy carbon electrode

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Chinese Chemical Letters 2014年第4期25卷 505-510页

作者： Yuan-Yuan Yao Long Zhang Zi-Fei Wang Jing-Kun Xu yang-ping wen Jiangxi Key Laboratory of Organic Chemistry Jiangxi Science and Technology Normal University College of Science Jiangxi Agricultural University

A simple and sensitive platinum nanoparticles/poly（hydroxymethylated-3,4-ethylenedioxylthiophene） nanocomposite （PtNPs/PEDOT-MeOH） modified glassy carbon electrode （GCE） was successfully developed for the electrochemical determination of quercetin. Scanning electron microscopy and energy dispersive X-ray spectroscopy results indicated that the PtNPs were inserted into the PEDOT- MeOH layer. Compared with the bare GCE and poly（3,4-ethylenedioxythiophene）（PEDOT） electrodes, the PtNPs/PEDOT-MeOH/GCE modified electrode exhibited a higher electrocatalytic ability toward the oxidation of quercetin due to the synergic effects of the electrocatalytic activity and strong adsorption ability of PtNPs together with the good water solubility and high conductivity of PEDOT-MeOH. The electrochemical sensor can be applied to the quantification of quercetin with a linear range covering 0.04-91μmol L-1 and a low detection limit of 5.2 nmol L-1. Furthermore, the modified electrode also exhibited good reoroducibilitv and long-term stability, as well as high selectivity.

关键词： Poly( 3 4-ethylenedioxylthiophene methanol) Platinum nanoparticles Electropolymerization Differential pulse voltammetryQuercetin

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POLY(3,4-ETHYLENEDIOXYTHIOPHENE METHANOL)/ASCORBATE OXIDASE/NAFION-SINGLE-WALLED CARBON NANOTUBES BIOSENSOR FOR VOLTAMMETRIC DETECTION OF VITAMIN C

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Chinese Journal of Polymer Science 2012年第3期30卷 460-469页

作者： yang-ping wen Dong Li Yao Lu 贺浩华徐景坤 Xue-min Duan Ming Liu Jiangxi Agr Univ Key Lab Crop Physiol Ecol&Genet BreedingMinist EducNanchang 330045Peoples R China Jiangxi Agr Univ Key Lab Physiol Ecol&Cultivat Double Cropping RMinist AgrNanchang 330045Peoples R China Jiangxi Sci&Technol Normal Univ Jiangxi Key Lab Organ ChemNanchang 330013Peoples R China Jiangxi Sci&Technol Normal Univ Sch PharmNanchang 330013Peoples R China

Vitamin C （VC） content in commercial juices was voltammetrically determined using a highly selective and sensitive poly（3,4-ethylenedioxythiophene methanol）/ascorbate oxidase/Nafion-single-walled carbon nanotubes （PEDOTM/AO/Nation-SWCNT） biosensor. The biocompatible PEDOTM matrix was prepared facilely by the one-step electrochemical deposition technique in lithium perehlorate aqueous solutions. AO was dip-coated on the surface of the biocompatible PEDOTM matrix. The mixture of Nafion-SWCNT was dip-cast onto the surface of AO layer when it was obtained by blending Nation solution and SWCNT dispersion together in a volume ratio of 1：1. The prepared PEDOTM/AO/Nafion-SWCNT biosensor was used for the voltammetric determination of VC, which exhibited the good linear range （4.0 ~ 10-s-3 x 10-s mol/L）, low detection limit （13 I^mol/L）, pronounced sensitivity （1.4072 mA （mmol/L） i cm 2）, high bioaftinity （low apparent Michaelis-Menten constant）, good stability （good repeatability）, high specificity （good anti-interference ability） coupled with the good reliability and feasibility （the determination of VC in commercial juices）. Meanwhile, the good aqueous solubility and the low onset oxidation potential of EDOTM will be more beneficial to the application in biosensor field compared to 3,4-ethylenedioxythiophene. Moreover, the good biocompatibility of PEDOTM matrix and high selectivity of Nation-SWCNT films also provide a promising platform for the development of biosensing devices.

关键词： Biosensor Vitamin C Poly(3,4-ethylenedioxythiophene methanol) Nation Single-walled carbon nanotubes.

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Ascorbate oxidase electrochemical biosensor based on the biocompatible poly(3,4-ethylenedioxythiophene) matrices for agricultural application in crops

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Chinese Chemical Letters 2012年第2期23卷 221-224页

作者： yang ping wen Li Min Lu Dong Li Ming Liu Hao Hua He Jing KunXu Key Lab of Crop Physiology Ecology and Genetic BreedingMinistry of EducationJiangxi Agricultural UniversityNanchang 330045China Jiangxi Key Lab of Organic Chemistry Jiangxi Science and Technology Normal UniversityNanchang 330013China

The vitamin C（VC） in crops was successfully determined using ascorbate oxidase（AO） electrochemical biosensor based on the biocompatible poly（3,4-ethylenedioxythiophene）（PEDOT） matrices,which was easily prepared by one-step electrodeposition technique in ionic liquid *** fabricated biosensor displayed excellent bioelectrocatalytic performance to the oxidation of VC,wide linear range,low detection limit,fast response time,good operational and storage stability,the good results of the determination of VC in vegetable crops indicated that the fabricated biosensor will be a good candidate for the physiological and biochemical studies of crops in near future.

关键词： Biosensor Agricultural application Ionic liquid microemulsions Ascorbate oxidase Poly(3 4-ethyl-enedioxythiophene) Vitamin C

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Electrochemical sensor based on f-SWCNT and carboxylic group functionalized PEDOT for the sensitive determination of bisphenol A

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Chinese Chemical Letters 2014年第4期25卷 517-522页

作者： Long Zhang yang-ping wen Yuan-Yuan Yao Zi-Fei Wang Xue-Min Duan Jing-Kun Xu School of Pharmacy Jiangxi Science & Technology Normal University Jiangxi Key Laboratory of Organic Chemistry Jiangxi Science and Technology Normal University

A simple, sensitive, and reliable method for the voltammetric determination of bisphenol A （BPA） by using carboxylic group functionalized single-walled carbon nanotubes （f-SWCNT）/carboxylic-functionalized poly（3,4-ethylenedioxythiophene）（PC4） complex modified glassy carbon electrode （GCE） has been successfully developed. The electrochemical behavior of BPA at the surface of the modified electrode is investigated by electrochemical techniques. The cyclic voltammetry results show that the as-prepared electrode exhibits strong catalytic activity toward the oxidation of BPA with a well-defined anodic peak at 0.623 V in PBS （0.1 mol/L, pH 7.0）. The surface morphology of the 3D network of composite film is beneficial for the adsorption ofanalytes. Under the optimized conditions, the oxidation peak current is proportional to BPA concentration in the range between 0.099 and 5.794 μmol/L （R2 = 0.9989）, with a limit of detection of 0.032 μmol/L （SIN = 3）. The enhanced performance of the sensor can be attributed to the excellent electrocatalytic property off-SWCNT and the extraordinary conductivity of PC4. Furthermore, the proposed modified electrode displays high stability and good reproducibility. The good result on the voltammetric determination of BPA also indicates that the asfabricated modified electrode will be a good candidate for the electrochemical determination and analysis of BPA.

关键词： Bisphenol A Electrochemical sensor Voltammetric determination Poly(3 4-ethylenedioxythiophene) derivative

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One-step co-electrodeposition of graphene oxide doped poly(hydroxymethylated-3,4-ethylenedioxythiophene) film and its electrochemical studies of indole-3-acetic acid

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Chinese Chemical Letters 2014年第4期25卷 511-516页

作者： Zi-Lan Feng Yuan-Yuan Yao Jing-Kun Xu Long Zhang Zi-Fei Wang yang-ping wen Jiangxi Key Laboratory of Organic Chemistry Jiangxi Science and Technology Normal University College of Science Jiangxi Agricultural University

A novel graphene oxide （GO） doped poly（hydroxymethylated-3,4-ethylenedioxythiophene）（PEDOTM） film has been achieved via one-step co-electrodeposition and utilized for electrochemical studies of indole-3-acetic acid （IAA）. The incorporation of GO into PEDOTM film facilitated the electrocatalytic activity and exhibited a favorable interaction between the PEDOTM/GO film and the phytohormone during the oxidation of IAA. Under optimized conditions, differential pulse voltammetry and square wave voltammetry were used for the quantitative analysis of IAA, respectively, each exhibiting a wide linearity range from 0.6 μmol L-1 to 10 μmol L-1 and 0.05 μmol L-1 to 40 μmol L-1, good sensitivity with a low detection Iimit of 0.087 μmol L-1 and 0.033μmol L T, respectively, as well as good stability. With the notable advantages of a green, sensitive method, expeditious response and facile operation, the as-prepared PEDOTM/GO organic-inorganic composite film provides a promising platform for electrochemical studies of IAA.

关键词： Co-electrodeposition EDOT derivatives Graphene oxide Indole-3-acetic acid

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注盐法整治季节性铁路路基冻害可行性试验研究

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科学技术与工程 2019年第10期19卷 204-209页

作者：杨文平神华包神铁路集团有限责任公司鄂尔多斯017000

以青藏铁路鸟岛段季节性冻土区路基冻害为研究背景,在室内填筑路基实体模型,进行封闭系统中反复冻融循环条件下的模型试验,分析冻融循环条件下普通路基和注盐路基的温度和位移规律。结果表明:随着环境温度的周期性变化,普通段和注盐段... 详细信息

以青藏铁路鸟岛段季节性冻土区路基冻害为研究背景,在室内填筑路基实体模型,进行封闭系统中反复冻融循环条件下的模型试验,分析冻融循环条件下普通路基和注盐路基的温度和位移规律。结果表明:随着环境温度的周期性变化,普通段和注盐段的温度均呈现出正弦曲线变化规律,试验段温度波动范围为环境波动范围的1/3左右;普通段两个断面路基土体在固结沉降过程中呈现出有规律的抬升和下沉,注盐段呈缓慢下沉;普通段和注盐段两个试验段四个断面路基土体冻胀最大值分别为0. 23 mm、0. 10 mm和0. 06 mm、0. 05 mm,注盐之后路基冻胀减小平均约74%,说明注盐对于路基土体冻胀有很大的抑制的作用,注盐法整治路基冻害是可行的。

关键词：铁路路基冻胀融沉模型试验注盐法可行性研究

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闭口型压型钢板—混凝土组合板承载能力试验研究

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建筑科学 2008年第5期24卷 37-42,12页

作者：杨文平杨勇华侨大学土木工程学院福建泉州362021

闭口型压型钢板—混凝土组合板中,压型钢板能更好地起到受拉钢筋的作用,比开口型压型钢板—混凝土组合板有更多性能优势和更好的应用前景。通过八块采用新型BONDEKⅡ型闭口型压型钢板的组合板的静力性能试验研究和理论分析,着重考察剪... 详细信息

闭口型压型钢板—混凝土组合板中,压型钢板能更好地起到受拉钢筋的作用,比开口型压型钢板—混凝土组合板有更多性能优势和更好的应用前景。通过八块采用新型BONDEKⅡ型闭口型压型钢板的组合板的静力性能试验研究和理论分析,着重考察剪跨比和组合板端部栓钉布置对组合板受力性能、破坏形态和极限承载能力的影响,提出了该类闭口型压型钢板的组合板的受弯承载力、竖向受剪承载力和纵向剪切粘结破坏承载力的计算方法及公式,为组合板设计计算提供依据。

关键词：闭口型压型钢板组合板承载能力试验研究

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无人机三维实时航迹规划仿真平台

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计算机仿真 2011年第8期28卷 41-46页

作者：杨文平李俨西北工业大学自动化学院陕西西安710129

无人机航迹规划是任务规划中的关键技术,为得到一条既安全可靠又全局代价最优的三维航迹。针对实现实时动态规划最佳航迹,利用图形用户界面开发环境,规则网格的数字高程地图模型建立了基于地形因素的多种威胁源模型,采用改进型概率地图... 详细信息

无人机航迹规划是任务规划中的关键技术,为得到一条既安全可靠又全局代价最优的三维航迹。针对实现实时动态规划最佳航迹,利用图形用户界面开发环境,规则网格的数字高程地图模型建立了基于地形因素的多种威胁源模型,采用改进型概率地图法(probabilistic roadmap method,PRM)与最短路径搜索—Dijkstra算法相结合的方法规划了三维航迹。最终设计的平台能够实现多种航迹规划算法的搜索,二维与三维、实时与非实时、单机与多机的航迹规划仿真。通过平台的实际运行,证明了改进PRM算法的高效性,同时验证了该平台的有效性与实用性。

关键词：无人机威胁源模型航迹规划仿真平台

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