检索结果-南通市图书馆

Single-cell RNA sequencing analysis of the retina under acute high intraocular pressure

Neural Regeneration Research 2024年第11期19卷 2522-2531页

作者： Shaojun Wang Siti Tong Xin Jin Na Li Pingxiu Dang yang Sui Ying Liu Dajiang Wang Division of Ophthalmology The Third Medical Center of PLA General HospitalBeijingChina Department of Ophthalmology Beijing Rehabilitation HospitalCapital Medical UniversityBeijingChina

High intraocular pressure causes retinal ganglion cell injury in primary and secondary glaucoma diseases,yet the molecular landscape characteristics of retinal cells under high intraocular pressure remain *** models of acute hypertension ocular pressure were established by injection of cross-linked hyaluronic acid hydrogel(Healaflow■).Single-cell RNA sequencing was then used to describe the cellular composition and molecular profile of the retina following high intraocular *** results identified a total of 12 cell types,namely retinal pigment epithelial cells,rod-photoreceptor cells,bipolar cells,Müller cells,microglia,cone-photoreceptor cells,retinal ganglion cells,endothelial cells,retinal progenitor cells,oligodendrocytes,pericytes,and *** single-cell RNA sequencing analysis of the retina under acute high intraocular pressure revealed obvious changes in the proportions of various retinal cells,with ganglion cells decreased by 23%.Hematoxylin and eosin staining and TUNEL staining confirmed the damage to retinal ganglion cells under high intraocular *** extracted data from retinal ganglion cells and analyzed the retinal ganglion cell cluster with the most distinct *** found upregulation of the B3gat2 gene,which is associated with neuronal migration and adhesion,and downregulation of the Tsc22d gene,which participates in inhibition of *** study is the first to reveal molecular changes and intercellular interactions in the retina under high intraocular *** data contribute to understanding of the molecular mechanism of retinal injury induced by high intraocular pressure and will benefit the development of novel therapies.

关键词： apoptosis axon degeneration high intraocular pressure microglia ocular hypertension photoreceptor cells retina retinal degeneration retinal ganglion cells single-cell RNA sequencing

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Metabolomic Analysis in Saliva and Different Brain Regions of Older Mice with Postoperative Delirium Behaviors

引用

Biomedical and Environmental Sciences 2024年第2期37卷 133-145页

作者： LIU Xiao CAO Ying LIN Xiao Wan GAO Dan yang MIAO Hui Hui LI Tian Zuo Department of Anesthesiology Beijing Shijitan HospitalCapital Medical UniversityBeijing 100038China

Objective Postoperative delirium(POD)has become a critical challenge with severe consequences and increased incidences as the global population ***,the underlying mechanism is yet *** study aimed to explore the changes in metabolites in three specific brain regions and saliva of older mice with postoperative delirium behavior and to identify potential non-invasive *** Eighteen-month-old male C57/BL6 mice were randomly assigned to the anesthesia/surgery or control *** tests were conducted 24 h before surgery and 6,9,and 24 h after *** C3(C3)and S100 calcium-binding protein B protein(S100beta)levels were measured in the hippocampus,and a metabolomics analysis was performed on saliva,hippocampus,cortex,and amygdala *** In total,43,33,38,and 14 differential metabolites were detected in the saliva,hippocampus,cortex,and amygdala,respectively.“Pyruvate”“alpha-linolenic acid”and“2-oleoyl-1-palmitoy-snglycero-3-phosphocholine”are enriched in one common pathway and may be potential non-invasive biomarkers for *** changes were observed in the three brain regions,with the upregulation of 1-methylhistidine and downregulation of *** Dysfunctions in energy metabolism,oxidative stress,and neurotransmitter dysregulation are implicated in the development of *** identification of changes in the level of salivary metabolite biomarkers could aid in the development of noninvasive diagnostic methods for POD.

关键词： Metabolomic Postoperative delirium(POD) Mechanism Biological marker

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A High-Contrast Autolysosome Probe for Detecting Interaction between Autophagosomes and Autolysosomes in Mitophagy

引用

Chinese Journal of Chemistry 2024年第8期42卷 858-866页

作者： Weihua Wang Chu Han Shengjie Xie Zisong Cong Zixuan yang Yuxin Feng Limin Xiang Heng Song College of Chemistry and Molecular Sciences Wuhan UniversityWuhanHubei430072 China

Comprehensive Summary,Autophagy is a multi-step cell metabolism process in which cells remove damaged and unwanted *** autophagy,autophagosomes fuse with lysosomes to form *** membrane components are recycled from autolysosomes through the autophagosomal components recycling(ACR),while lysosomal components circulate on the autolysosomal surface through the autophagic lysosome reformation(ALR)*** contain components from autophagosomes and ***,whether there is a fusion between autolysosome and autophagosome or lysosome at the organelle level remains *** this study,a pH and viscosity dual-controlled mitochondria-targeting fluorescent probe Mito-Q was designed based on an asymmetric norcyanine to achieve the high-contrast imaging of mitochondria-containing ***-Q not only effectively detected mitochondrial viscosity changes and mitophagy with high sensitivity,but more importantly,the fusion of mitochondria-containing autolysosomes and autophagosomes(FMAA)was observed during autophagy by the real-time confocal imaging of HeLa cells.

关键词： Mitophagy Autolysosomes Bioimaging Biosensors Fluorescence imaging

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Hexokinase 1 and 2 mediates glucose utilization to regulate the synthesis of kappa casein via ribosome protein subunit 6 kinase 1 in bovine mammary epithelial cells

引用

Animal Nutrition 2024年第1期16卷 338-349页

作者： Tianyu yang Jia Guo Han Song Osmond Datsomor Yuhang Chen Maocheng Jiang Kang Zhan Guoqi Zhao Institute of Animal Culture Collection and Application College of Animal Science and Technology Yangzhou University

Glucose plays a vital part in milk protein synthesis through the mTOR signaling pathway in bovine mammary epithelial cells(BMEC). The objectives of this study were to determine how glucose affects hexokinase(HK) activity in BMEC and investigate the regulatory effect of HK in kappa casein(CSN3)synthesis via the mechanistic target of rapamycin complex 1(mTORC1) signaling pathway in BMEC. For this, HK1 and HK2 were knocked out in BMEC using the CRISPR/Cas9 system. The gene and protein expression, glucose uptake, and cell proliferation were measured. We found that glucose uptake, cell proliferation, CSN3 gene expression levels, and expression of HK1 and HK2 increased with increasing glucose concentrations. Notably, glucose uptake was significantly reduced in HK2 knockout(HK2KO)BMEC treated with 17.5 mM glucose. Moreover, under the same glucose treatment conditions, the proliferative ability and abundance of CSN3 were significantly diminished in both HK1 knockout(HK1KO)and HK2KO BMEC compared with that in wild-type BEMC. We further observed that the phosphorylation levels of ribosome protein subunit 6 kinase 1(S6K1) were reduced in HK1KO and HK2KO BMEC following treatment with 17.5 mM glucose. As expected, the levels of glucose-6-phosphate and the m RNA expression levels of glycolysis-related genes were decreased in both HK1KO and HK2KO BMEC following glucose treatment. These results indicated that the knockout of HK1 and HK2 inhibited cell proliferation and CSN3 expression in BMEC under glucose treatment, which may be associated with the inactivation of the S6K1 and inhibition of glycolysis.

关键词： Glucose Hexokinase Milk protein Mechanistic target of rapamycin complex 1 signaling pathway Bovine mammary epithelial cell Kappa casein

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Mechanical behaviors of backfill-rock composites: Physical shear test and back-analysis

引用

Journal of Rock Mechanics and Geotechnical Engineering 2024年第3期16卷 807-827页

作者： Jie Xin Quan Jiang Fengqiang Gong Lang Liu Chang Liu Qiang Liu Yao yang Pengfei Chen State Key Laboratory of Geomechanics and Geotechnical Engineering Institute of Rock and Soil MechanicsChinese Academy of SciencesWuhan430071China University of Chinese Academy of Sciences Beijing100049China School of Civil Engineering Southeast UniversityNanjing211189China Energy School Xi’an University of Science and TechnologyXi’an710054China

The shear behavior of backfill-rock composites is crucial for mine safety and the management of surface *** exposing the shear failure mechanism of backfill-rock composites,we conducted shear tests on backfill-rock composites under three constant normal loads,compared with the unfilled *** investigate the macro-and meso-failure characteristics of the samples in the shear tests,the cracking behavior of samples was recorded by a high-speed camera and acoustic emission *** parallel with the experimental test,the numerical models of backfill-rock composites and unfilled rock were established using the discrete element method to analyze the continuous-discontinuous shearing *** on the damage mechanics and statistics,a novel shear constitutive model was proposed to describe mechanical *** results show that backfill-rock composites had a special bimodal phenomenon of shearing load-deformation curve,*** first shearing peak corresponded to rock break and the second shearing peak induced by the broken of aeolian sand-cement/fly ash paste ***,the shearing characteristic curves of the backfill-rock composites could be roughly divided into four stages,*** shear failure of the specimens experienced:stage I:stress concentration;stage II:crack propagation;stage III:crack coalescence;stage IV:shearing *** numerical simulation shows that the existence of aeolian sand-cement/fly ash paste backfill inevitably altered the coalescence type and failure mode of the specimens and had a strengthening effect on the shear strength of backfillrock *** on damage mechanics and statistics,a shear constitutive model was proposed to describe the shear fracture characteristics of specimens,especially the bimodal ***,the micro-and meso-mechanisms of shear failure were discussed by combining the micro-test and numerical *** research can advance the better understanding of the shear behavior of

关键词： Physical simulation Backfill-rock composites Shear failure Cracking Shear constitutive model

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

元宇宙背景下服装场景特性对购买意愿的影响研究——基于感知价值与临场感的中介效应

引用

丝绸 2024年第1期61卷 86-95页

作者：谭孝勤李沛上海工程技术大学纺织服装学院上海201620

移动互联时代下场景营销成为企业营销取胜的关键,元宇宙概念的提出也为服装行业带来更丰富的互动入口。为探索服装场景特性在元宇宙背景下对消费者的作用机制,本文通过文献归纳及案例分析,从体验视角将元宇宙背景下服装场景的特性划分... 详细信息

移动互联时代下场景营销成为企业营销取胜的关键,元宇宙概念的提出也为服装行业带来更丰富的互动入口。为探索服装场景特性在元宇宙背景下对消费者的作用机制,本文通过文献归纳及案例分析,从体验视角将元宇宙背景下服装场景的特性划分为场景依赖性、信息精准性、社会交互性、场景娱乐性四个特性,并构建理论模型。基于问卷调研数据,本文采用因子分析、回归分析和结构型模型结合的方法,实证检验了元宇宙背景下服装场景特性对购买意愿的影响。研究发现:场景依赖性、信息精准性、社会交互性和场景娱乐性、感知价值、临场感与购买意愿显著正相关;感知价值和临场感在服装场景特性与购买意愿之间具有中介作用;其中,消费者感知价值的中介效应较强。本文据此对企业在元宇宙背景下的场景营销战略提出对策与建议。

关键词：元宇宙服装场景特性感知价值临场感购买意愿回归分析

来源：

维普期刊数据库

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

Dysregulated N6-methyladenosine modification in peripheral immune cells contributes to the pathogenesis of amyotrophic lateral sclerosis

引用

Frontiers of Medicine 2024年第2期18卷 285-302页

作者： Di He Xunzhe yang Liyang Liu Dongchao Shen Qing Liu Mingsheng Liu Xue Zhang Liying Cui Department of Neurology Peking Union Medical College Hospital(PUMCH)Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing100730China Medical Doctor Program Chinese Academy of Medical Science and Peking Union Medical CollegeBeijing100730China McKusick-Zhang Center for Genetic Medicine State Key Laboratory of Medical Molecular BiologyInstitute of Basic Medical Sciences Chinese Academy of Medical SciencesSchool of Basic MedicinePeking Union Medical CollegeBeijing100730China Neuroscience Center Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing100730China

Amyotrophic lateral sclerosis(ALS)is a progressive neurogenerative disorder with uncertain *** evidence implicates N6-methyladenosine(m6A)modification in ALS *** RNA immunoprecipitation sequencing(MeRIP-seq)and liquid chromatography–mass spectrometry were utilized for m6A profiling in peripheral immune cells and serum proteome analysis,respectively,in patients with ALS(n=16)and controls(n=6).The single-cell transcriptomic dataset(GSE174332)of primary motor cortex was further analyzed to illuminate the biological implications of differentially methylated genes and cell communication *** of peripheral immune cells revealed extensive RNA hypermethylation,highlighting candidate genes with differential m6A modification and expression,including C-X3-C motif chemokine receptor 1(CX3CR1).In RAW264.7 macrophages,disrupted CX3CR1 signaling affected chemotaxis,potentially influencing immune cell migration in *** proteome analysis demonstrated the role of dysregulated immune cell migration in *** type-specific expression variations of these genes in the central nervous system(CNS),particularly microglia,were *** communication between neurons and glial cells was selectively altered in ALS *** integrated approach underscores m6A dysregulation in immune cells as a potential ALS contributor.

关键词： amyotrophic lateral sclerosis N^(6)-methyladenosine epi-transcriptome proteome single cell RNA sequencing analysis CX3CR1

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

How Rustic Videos Influence Youth’s linguistic Expression: An Analysis Based on Semiotics, Meme Theory and Media Spectacle Theory

引用

Open Journal of Applied Sciences 2024年第7期14卷 1648-1665页

作者： Jiaxin yang Faculty of Arts and Social Science The University of Sydney Sydney Australia

The surge in popularity of rustic videos has spawned a great number of Internet memes, such as Internet trendy words growing from dialects and strange pronunciations, picture memes made from video screenshots, and mesmerizing music with a vernacular flavor. Due to their reproducibility, social interaction, and involvement, these rustic videos adhere to the fundamental logic of the propagation of online memes. Rustic videos are widely disseminated as online memes on TikTok (the Chinese version), are often reproduced and used by young people in social contact, and have become a unique linguistic symbol in modern internet culture. As a symbolic carrier that transports the consciousness of the video creator and viewer, it is widely employed in the communication and engagement of young people on a regular basis, progressively altering their linguistic expression. This specific semiotic interaction has deconstructed and recreated the conventional media culture spectacle. This research examines the influence of rustic videos on TikTok on the linguistic expressions of modern youth from the perspectives of meme theory and semiotics, as well as the impact of rustic videos on the media spectacle from the standpoint of media spectacle theory. It also examines in depth the effects of the popularity of rustic videos on China’s economy and culture.

关键词： Rustic Videos linguistic Expression Semiotics Meme Media Spectacle

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The Media Logic and Cultural Impact of WeChat

引用

Open Journal of Applied Sciences 2024年第7期14卷 1754-1761页

作者： Jiaxin yang The Faculty of Arts and Social Science The University of Sydney Sydney Australia

Through its media logic, which is defined by programmability, pervasiveness, connectivity, and datafication, WeChat establishes a non-linear, interactive, and user-dominated media environment. This media logic enhances the influence of each user within WeChat’s narrative landscape and revolutionises traditional media narration methods by enabling them to generate and distribute content. In such an environment, users are able to edit, receive, and send information without constraints of time or distance, which enables delayed interactions that broaden the potential for human communication and dissemination. Additionally, WeChat partially replaces social behaviours and institutions, thereby modifying their original structures and characteristics. As individuals become more accustomed to this media environment, they progressively adjust to the forms that are appropriate for media representation on WeChat, thereby completing the mediatization of culture and society. In this process, individuals are becoming more dependent on media and media logic, with WeChat’s influence permeating social and cultural activities through its media logic. This interaction has a substantial impact on society and culture, thereby aiding in their reconstruction.

关键词： Social Media Media Logic Non-Linear Narratives User Engagement Mediatization

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

硬岩小净距隧道先行洞的爆破振动响应规律

引用

中南大学学报（自然科学版） 2024年第9期55卷 3354-3367页

作者：陈璐曹洪涛周子龙曾铃高山刘洋凌同华聂森林长沙理工大学土木工程学院湖南长沙410114 中南大学资源与安全工程学院湖南长沙410083 广西新发展交通集团有限公司广西南宁530029 精细爆破国家重点实验室湖北武汉430056

隧道爆破施工时,爆破破岩产生的振动效应对近邻隧道结构稳定性影响较大。为提高隧道爆破施工的安全性,基于广西某双线硬岩小净距隧道爆破开挖工程,通过现场爆破振动监测,分析后行洞爆破开挖时爆破振动的传播规律与质点峰值振动速度的分... 详细信息

隧道爆破施工时,爆破破岩产生的振动效应对近邻隧道结构稳定性影响较大。为提高隧道爆破施工的安全性,基于广西某双线硬岩小净距隧道爆破开挖工程,通过现场爆破振动监测,分析后行洞爆破开挖时爆破振动的传播规律与质点峰值振动速度的分布特征,并利用有限元软件LS-DYNA建立相应的数值模型,探索近邻先行隧道径向和轴向上质点峰值振动速度演化特征及分布规律,基于最大主应力原则,构建近邻隧道爆破振动安全判据。研究结果表明:对后行隧道爆破开挖时,水平径向的振动波对近邻隧道的影响起主导作用;在爆心距相同的情况下,隧道未开挖侧的爆破振动衰减速度比已开挖侧的小;近邻隧道迎爆侧边墙、拱脚和底脚是主要受扰动区,边墙的质点峰值振动速度最大,在施工中应当加强边墙的监测和防护。基于近邻隧道质点峰值振动速度与拉应力内在联系的回归分析,并考虑混凝土的动态抗拉强度,建议将本隧道爆破施工的质点峰值振动速度阈值确定为15.7 cm/s,以保证依托工程近邻隧道支护结构的安全。

关键词：近邻隧道质点振动峰值速度爆破振动时空响应

来源：

维普期刊数据库博看期刊

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：