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Knockdown of the Premature Ovarian Insufficiency Candidate Gene NUP107 in Ovarian Granulosa Cells Affects Cell Functions, Including Receptor Expression and Estrogen Synthesis

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Reproductive and Developmental Medicine 2019年第3期3卷 133-140,I0001页

作者： Xiao-Cheng Liu Meng-Xin Han yan Xu hong-yan wang Bin Li Department of Obstetrics and Gynecology Obstetrics and Gynecology Hospital of Fudan UniversityShanghai 200011China Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases Fudan UniversityShanghai 200011China The Institute of Reproduction and Development Obstetrics and Gynecology HospitalInstitute of Reproduction and DevelopmentFudan UniversityShanghai 200438China

Objective:Mutations in NUP107 have been discovered in patients with premature ovarian insufficiency and may have tissue-specific effects in ovarian ***,the role of NUP107 in human granulosa cell(GC)function and female fertility still remains *** this study,we used RNA interference to investigate how NUP107 dysfunction influences GCs and ovarian ***:Immunohistochemical staining was used to detect the expression of NUP107 in *** counting kit-8 assay,real-time cell analysis,and flow cytometry were used to explore cell proliferation and apoptosis,and an enzyme-linked immunosorbent assay was used to assess the estrogen *** real-time reverse transcription-quantitative polymerase chain reaction and Western blot analyses were used to determine the expression of NUP107 and functional ***:Knockdown of NUP107 expression had little effect on the growth and number of *** study confirmed that knockdown of NUP107 may interfere with estrogen synthesis in GCs and their sensitivity to the regulation of follicle-stimulating hormone(FSH)by decreasing the expression of estrogen synthesis-related genes AR,CYP17A1,CYP19A1,STAR,and ***,knockdown of NUP107 decreased the expression of AMHR2,FSHR,LHR,and ESR1 in GCs,but had no effect on the expression of ***:These data revealed that NUP107 may impede follicle growth and maturation by regulating hormone synthesis,sensitivity to follicle-stimulating hormone,and expression of functional receptors in GCs,and may,therefore,interfere with female fertility.

关键词： Estrogen NUP107 Ovarian Granulosa Cells Premature Ovarian Insufficiency

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Safety and efficacy of coronary drug eluting stent for atherosclerotic stenosis of the small renal artery

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Chinese Medical Journal 2009年第10期122卷 1223-1226页

作者： LI Chun-jie WU Zheng yan hong-bing wang Jian ZHAO Han-jun The 28th Division Beijing Anzhen Hospital Capital Medical University Beijing 100029 China

Small diameter renal artery refers to the renal artery with the cross-section diameter less than 5 mm, the incidence of which is approximately 8%＇ Small diameter renal artery is common in patients with congenital multi-branch renal arteries, diabetes and multi-coronary artery lesions. Renal artery bare-mental stent （BMS） implantation is the standard treatment for ostial renal

关键词： drug eluting stent atherosclerotic stenosis small renal artery

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O-O bond formation mechanisms during the oxygen evolution reaction over synthetic molecular catalysts

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Chinese Journal of Catalysis 2021年第8期42卷 1253-1268页

作者： Xue-Peng Zhang hong-yan wang Haoquan Zheng Wei Zhang Rui Cao Key Laboratory of Applied Surface and Colloid Chemistry Ministry of EducationSchool of Chemistry and Chemical EngineeringShaanxi Normal UniversityXi’an 710119ShaanxiChina

Water oxidation is one of the most important reactions in natural and artificial energy conversion *** nature,solar energy is converted to chemical energy via water oxidation at the oxygen-evolving center of photosystem II to generate dioxygen,protons,and *** artificial energy schemes,water oxidation is one of the half reactions of water splitting,which is an appealing strategy for energy conversion via photocatalytic,electrocatalytic,or photoelectrocatalytic *** it is thermodynamically unfavorable and kinetically slow,water oxidation is the bottleneck for achieving large-scale water ***,developing highly efficient water oxidation catalysts has attracted the interests of researchers in the past *** formation of O-O bonds is typically the rate-determining step of the water oxidation catalytic ***,better understanding this key step is critical for the rational design of more efficient *** review focuses on elucidating the evolution of metal-oxygen species during transition metal-catalyzed water oxidation,and more importantly,on discussing the feasible O-O bond formation mechanisms during the oxygen evolution reaction over synthetic molecular catalysts.

关键词： Oxygen evolution reaction Water oxidation O-O bond formation Transition metal complex Molecular electrocatalysis Reaction mechanism

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Frequency of infarct-related artery with myocardial bridging in patients with ST-elevation myocardial infarction and its impact upon percutaneous coronary intervention

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Chinese Medical Journal 2006年第7期119卷 539-543页

作者： yan hong-bing wang Jian ZHU Xiao-ling GAO Hal LI Nan AI Hui Center for Heart Lung and Blood Vessel DiseasesAnzhen Hospital Capital University of Medical Sciences Beijing100029 China

Background Myocardial bridging （MB） as a congenital condition with a reported frequency of 5%-12% in diagnostic coronary angiography may be an important factor causing myocardial ischemia. However, its frequency in the infarct-related artery （IRA） of patients with ST-elevation myocardial infarction （STEMI） and the impact upon percutaneous coronary intervention （PCI） remain undetermined. In this study, we investigated MB frequency and its impact upon primary PCI in patients with STEMI. Methods The data of coronary angiography for 554 consecutive patients with STEMI who had undergone successful primary PCI were retrospectively analyzed to identify a frequency of MB in the IRA and its association with gender and age. According to the angiographic findings, the patients were divided into MB patients and non-MB patients. The endpoints of this study included immediate angiographic findings after primary PCI and 6-month major adverse cardiac events （MACE）（death, recurrent myocardial infarction, target lesion or vessel revascularization） between the MB patients and the non-MB patients. Results A frequency of MB in the IRA of 46 patients （8.3%） was identified in this series; it was more common in patients ≥65 years old （36/206） than in those 〈65 years old （10/348）（17.5% vs 2.9%, P〈0.001）. The trend of MB in the IRA was observed more frequently in women without significant difference than in men （10.2% vs 7.8%）. TIMI grade Ⅲ flow was achieved in 91.9% （509/554） of all patients following primary PCI, in 60.9% （28/46） of the MB patients and in 94.7% （481/508） of the non-MB patients respectively （P〈0.001）. The in-hospital mortality was 4.7% （26/554） in this series including 13.0% （6/46） of the MB patients and 3.9% （20/508） of the non-MB patients （P〈0.001）. A significant difference in 6 months MACE was seen between the MB patients （19%） and the non-MB patients （6.2%）（P〈0.001）. Conclusions MB in the IRA is relatively common in elderly patients with STEMI with a more evide

关键词： myocardial bndging myocardial infarction percutaneous coronary intervention

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Editorial

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Defence Technology（防务技术） 2014年第2期10卷 84-85页

作者： Hai-bin wang yan-hong wang Yong-xiang LI Yu-cun LIU School of Chemical and Environmental Engineering North University of China

2,6-diamino-3,5-dinitropyrazine-1-oxide(ANPZO), as an insensitive high explosive, with a high yield and excellent purity has been prepared at pilot plant scale by an improved method. The synthesized ANPZO is characterized by IR, laser granularity measurement, SEM and *** particle analysis revealed that the improved method could offer desired product with average particle size of 40 mm and high purity(>98.45%). The experimental parameters exhibited that the detonation velocity of the formulation based on ANPZO was higher than that of the corresponding TATB formulation. The DSC curve showed that the exothermic decomposition of the product occurred at the temperature between300.5 C and 360.4 C. Furthermore, the sensitivity test suggests its safe nature towards mechanical stimulus.

关键词：国际研讨会国防科技科学技术弹道学组成部分工程师科学家

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Establishment and Application of a TaqMan Real-Time Quantitative Reverse Transcription-Polymerase Chain Reaction Assay for Rubella Virus RNA

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Acta Biochimica et Biophysica Sinica 2006年第10期38卷 731-736页

作者： Li-hong ZHAO~1* Yu-yan MA~2 hong wang~1 Shu-Ping ZHAO~3 Wei-Ming ZHAO~1 Hua LI~2 Lei-Yi wang~2 1 Department of Microbiology,School of Medicine,Shandong University,Jinan 250012,China 2 Qilu Hospital,Shandong University,Jinan 250012,China 3 Tai'an central Hospital,Tai'an 271000,China 4 Department of Virology,School of Public Health,Shandong University,Jinan 250012,China Department of Microbiology School of Medicine Shandong UniversityJinan 250012 China Qilu Hospital Shandong UniversityJinan 250012 China Tai'an central Hospital Tai'an 271000 China

The aim of this study was to establish and apply a real-time quantitative reverse transcription-polymerase chain reaction （RT-PCR） for rubella virus （RV） ***,the primer and TaqMan probeconcentrations,as well as reaction temperatures were optimized to establish an efficient real-time quantitativeRT-PCR assay for RV ***,an RV-specific PCR amplicon was made as an external standard toestimate the linearity,amplification efficiency,analytical sensitivity and reproducibility of the real timequantitative ***,the assay was applied to quantify RV RNA in clinical samples for rubella *** RV-specific PCR amplicon was prepared for evaluation of the assay at 503 bp,and its original concentrationwas 2.75×10～9 copies/μ*** real time quantitative assay was shown to have good linearity （R2=0.9920）,highamplification efficiency （E=1.91）,high sensitivity （275 copies/ml）,and high reproducibility （variation coefficientrange,from 1.25% to 3.58%）.Compared with the gold standard,the specificity and sensitivity of the assayin clinical samples was 96.4% and 86.4%,***,the established quantitative RT-PCRmethod is a simple,rapid,less-labored,quantitative,highly specific and sensitive assay for RV RNA.

关键词： rubella virus PCR amplicon real-time fluorescence quantitative RT-PCR

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Molecular Analysis of Genetic Fidelity in Micropropagated Plants of <i>Stevia rebaudiana</i>Bert. Using ISSR Marker

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American Journal of Plant Sciences 2013年第5期4卷 964-971页

作者： Hemant Lata Suman Chandra Natascha Techen yan-hong wang Ikhlas A. Khan National Center for Natural Product Research Research Institute of Pharmaceutical Sciences School of Pharmacy The University of Mississippi Oxford USA

Inter-simple sequence repeat (ISSR) markers were used to evaluate the genetic fidelity of in vitro propagated and hardened plants of Stevia rebaudiana Bert. Nodal segments containing axillary buds were used as explant and inoculated on Murashige and Skoog’s (MS) medium containing 3% (w/v) sucrose, 0.8% (w/v) agar supplemented with various concentrations of benzyladenine (BA), kinetin (Kn) and thidiazuron (TDZ) ranging from 0.20 to 2.00 mg·L-1. Maximum multiple shoots (93%) were obtained in MS medium supplemented with 0.20 mg L-1 TDZ. The best in vitro root induction (87%) was achieved on half strength MS medium without any growth regulator. The rooted plantlets were successfully established in soil and grown to maturity at the survival rate of 96% in the indoor grow room. For ISSR analysis, total genomic DNA was extracted from 20 mg fresh leaves of mother and randomly selected in vitro propagated plants. Out of? fifteen arbitrary primers tested, each produced clear and scorable amplification products ranged in size from about 216 bp in UBC 811 to 1917 bp in (GGGGT)3M with an average of 4.5 products per primer. A total of 45 bands (number of plantlets analyzed multiplied by number of bands with all primers) were generated by the ISSR method. All the ISSR profiles from micropropagated plants were monomorphic and comparable to mother plants, confirming the genetic stability among micropropagated plants and mother plant. Chemical analysis, using high-performance liquid chromatography (HPLC), was done to further confirm the existence of qualitative and quantitative differences in the major secondary metabolites (rebaudioside A, stevioside and steviolbioside) between the mother plant and in vitro propagated plants. Our results clearly show similar chemical profiles and insignificant differences in the major secondary metabolites between the two types of plants. These results suggest that the micropropagation protocol followed in this study is appropriate and applicable fo

关键词： Asteraceae Genetic Fidelity HPLC ISSR Micropropagation Stevia rebaudiana Stevioside

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TDZ-Induced High Frequency Plant Regeneration through Direct Shoot Organogenesis in <i>Stevia rebaudiana</i>Bertoni: An Important Medicinal Plant and a Natural Sweetener

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American Journal of Plant Sciences 2013年第1期4卷 117-128页

作者： Hemant Lata Suman Chandra yan-hong wang Vijayasankar Raman Ikhlas A. Khan National Center for Natural Products Research School of Pharmacy The University of Mississippi University USA

An efficient high frequency plant regeneration protocol through direct organogenesis was developed for Sevia rebaudiana Bert. Nodal segments containing axillary buds were used as an explant and inoculated on Murashige and Skoog’s (MS) medium containing 3% (w/v) sucrose, 0.8% (w/v) agar supplemented with various concentrations of benzyladenine (BA), kinetin (Kn) and thidiazuron (TDZ) ranging from 1.00 to 9.00 μM. Maximum multiple shoots (96%) were obtained in MS medium supplemented with 1.0 μM TDZ with an average of 60 shoots per culture, having an average shoot length of 6.0 cm. The best in vitro root induction (89%) was achieved on half strength MS medium without any growth regulator with an average of 24 roots per culture and root length of7 cm. The rooted plantlets were successfully established in soil and grown to maturity at the survival rate of 95% in the indoor grow room. High-performance liquid chromatography was used to assess the stability in chemical profile and quantification of stevioside and rebaudioside A content of in vitro propagated S. rebaudiana plants and compared with their mother plant at the peak vegetative stage. Our results show no significant differences (p in vitro propagated plants. Furthermore, fully developed in vitro propagated S. rebaudiana plants were also compared with mother plant for their gas and water vapour exchange characteristics and leaf anatomy. The results show that in vitro propagated and hardened plants of S. rebaudiana are morphologically as well as functionally comparable to each other and to their mother plant.

关键词： Asteraceae Gas and Water Vapour Exchange In Vitro Propagation Leaf Anatomy Rebaudioside A Stevia rebaudiana Stevioside Thidiazuron

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Single-cell-resolution transcriptome map revealed novel genes involved in testicular germ cell progression and somatic cells specification in Chinese tongue sole with sex reversal

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Science China(Life Sciences) 2023年第5期66卷 1151-1169页

作者： hong-yan wang Xiang Liu Jian-yang Chen Yingyi Huang Yifang Lu Fujian Tan Qun Liu Mingming yang Shuo Li Xianghui Zhang Yating Qin Wenxiu Ma Yingming yang Liang Meng Kaiqiang Liu Qian wang Guangyi Fan Rafael H.Nóbrega Shanshan Liu Francesc Piferrer Changwei Shao Key Lab of Sustainable Development of Marine Fisheries Ministry of Agriculture and Rural AffairsYellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266072China Laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and TechnologyQingdao 266071China College of Fisheries and Life Science Shanghai Ocean UniversityShanghai 201306China BGI Research-Qingdao BGIQingdao 266555China BGI-Shenzhen Shenzhen 518083China School of Marine Sciences Ningbo UniversityNingbo 315211China College of Marine Technology and Environment Dalian Ocean UniversityDalian 116023China Qingdao-Europe Advanced Institute for Life Sciences BGI-ShenzhenQingdao 266555China College of Life Sciences Shandong Normal UniversityJinan 250014China Reproductive and Molecular Biology Group Department of Structural and Functional BiologyInstitute of BiosciencesSão Paulo State University(UNESP)BotucatuSão PauloBrazil Institut de Ciències del Mar(ICM) Spanish National Research Council(CSIC)BarcelonaSpain

Female-to-male sex reversals(pseudomales)are common in lower vertebrates and have been found in natural populations,which is a concern under rapid changes in environmental *** can exhibit altered ***,the regulatory mechanisms underlying pseudomale spermatogenesis remain ***,we characterized spermatogenesis in Chinese tongue sole(Cynoglossus semilaevis),a species with genetic and environmental sex determination,based on a highresolution single-cell RNA-seq atlas of cells derived from the testes of genotypic males and *** identified five germ cell types and six somatic cell types and obtained a single-cell atlas of dynamic changes in gene expression during spermatogenesis in Chinese tongue sole,including alterations in *** detected decreased levels of Ca^(2+)signaling pathway-related genes in spermatogonia,insufficient meiotic initiation in spermatocytes,and a malfunction of somatic niche cells in ***,a cluster of CaSR genes and MAPK signaling factors were upregulated in undifferentiated spermatogonia of ***,we revealed that Z chromosome-specific genes,such as piwil2,dhx37,and ehmt1,were important for *** results improve our understanding of reproduction after female-to-male sex-reversal and provide new insights into the adaptability of reproductive strategies in lower vertebrates.

关键词： Cynoglossus semilaevis spermatogenesis single-cell transcriptome sex reversal spermatogonia

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A Systematic Review and Meta-Analysis on Gualou Niubang Decoction for Acute Mastitis

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Journal of Clinical and Nursing Research 2022年第1期6卷 59-66页

作者： Tian-tian Gao Shuo Zhang Zhen-lin Chen Miao wang hong-yan wang Wen-jing Ma Lu wang Shannxi University of Chinese Medicine Xianyang 712046Shannxi ProvinceChina Beijing University of Chinese Medicine Beijing 100029China The Second Affiliated Hospital of Shannxi University of Chinese Medicine Xianyang 712000Shannxi ProvinceChina

Objective:To systematically evaluate the clinical efficacy of Gualou Niubang decoction in the treatment of acute ***:Randomized controlled trials of Gualou Niubang decoction in the treatment of acute mastitis included in PubMed,Springer,Embase,Cochrane Library,CNKI,Wanfang,Weipu,and China Biomedical Literature Database from January 2016 to *** Cochrane risk bias assessment tool was used to evaluate the risk of *** Revman 5.3 software for Meta ***:There are 8 articles that meet the inclusion criteria,comprising 919 subjects,the test group were 462,cases and control group were 457 *** results of statistical analysis showed that the clinical efficacy of the experimental group was significantly higher than which of the control group,[RR=1.19,95%CI(1.07,1.33),P=0.002],the difference was statistically *** the secondary outcome indicators,compared to the control group,Gualou Niubang decoction can effectively relieve the main symptoms of acute mastitis[MD=-1.96,95%CI(-2.27,-1.65),P<0.0001].Gualou Niubang decoction combined with antibiotics can shorten the length of hospital stay[MD=-5.23,95%CI(-6.02,-4.44),P<0.00001]and reduce white blood cell count[MD=-2.40,95%CI(-4.71,-0.08),P=0.04]are better than ***:The use of Gualou Niubang decoction to treat acute mastitis can not only improve clinical efficacy,but also effectively relieve clinical symptoms,shorten hospital stay and reduce white blood cell count.

关键词： Gualou Niubang decoction Acute mammary mastitis Milk carbuncle Meta analysis

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