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The Susceptibilities of Oxya chinensis(Orthoptera:Acridoidea) to Malathion and Comparison of the Esterase Properties from Three Collected Populations in Tianjin Area,China

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Agricultural Sciences in China 2009年第1期8卷 76-82页

作者： WU Hai-hua yang mei-ling GUO Ya-ping MA En-bo Institute of Applied Biology Shanxi University Taiyuan 030006 P.R.China

The susceptibilities of Oxya chinensis to malathion were studied in three populations collected from outskirt of Tianjin, China, using bioassays and biochemical analysis. Populations were chosen to represent different exposure to insecticides： BDG （Beidagang; low exposure）, BD （Baodi; high exposure previously but low exposure now）, and JN （Jinnan; high exposure）. The results showed that the LD50 values of BD and JN populations were 3.95- and 12.02-fold and 3.64- and 10.07- fold higher than that of BDG population in females and males, respectively. The LD50 values in females were higher than those in males. The results of biochemical analysis indicated that the esterase （EST） activities in JN population were higher than those in BD and BDG populations. They showed that when α-NA, α-NB, and α-NA were used as substrates, females＇ EST activities of JN population were 1.11-, 1.30-, and 1.14-fold and 1.39-, 1.59-, and 1.54-fold higher than those of BD and BDG populations, respectively. When α-NA, α-NB, and β-NA were used as substrates, males＇ EST activities of JN population were 1.13-, 1.12-, and 1,00-fold and 1.20-, 1.14-, and 1.07-fold higher than those of BD and BDG populations, respectively. The results also showed that the specific activities of the females were higher than those of the males in the BD and JN populations, whereas the specific activities of the males were higher than those of the females in the BDG population. The results of bioassay were consistent with those of biochemical analysis. Thus, it was inferred that the elevated ESTs activities might play an important role in conferring the differences of susceptibility of O. chinensis to malathion in the three collected populations. Enzyme kinetic studies indicated that the Km and Vmax values were different among the three collected populations and between the females and the males. The observed changes in the kinetic parameters might be explained by differential expression patterns of isozymes so that th

关键词： Oxya chinensis esterase enzyme kinetics malathion susceptibility

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A Photosensitivity Insecticide, 5-Aminolevulinic Acid, Exerts EffectiveToxicity to Oxya chinensis (Orthoptera: Acridoidea)

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Agricultural Sciences in China 2011年第7期10卷 1056-1063页

作者： yang mei-ling YIN Kun GUO Ya-ping MA En-bo ZHANG Jian-zhen Research Institute of Applied Biology Shanxi University Taiyuan 030006 P.R.China Environmental Monitoring of China Beijing 100012 P.R.China School of Life Science and Technology Shanxi University Taiyuan 030006 P.R.China

5-Aminolevulinic acid （ALA）, a major photosensitivity insecticide, has attracted increasing attention as a new type of highly efficient, environmental friendly pesticide to be used to control the pest. To examine whether or not ALA acts effectively to grasshopper, Oxya chinensis and elucidate the detoxification mechanism of ALA, the susceptibility to ALA was assessed in O. chinensis and two major metabolic detoxification enzymes including glutathione S-transferases （GSTs） and general esterases （ESTs）-specific activities were compared in different development stages and different body sections of O. chinensis treated by ALA and the control. The results showed that the ALA exhibited obvious toxicity to the grasshopper in different development stages. In the low-dose treatment （0.0597 mmol L-1）, the mortalities of O. chinensis reached a significant level （55.5% in the 1st instar nymphs, 61.4% in the 2nd instar nymphs, 71.4% in the 3rd instar nymphs, and 64.4% in the 4th instar nymphs. But, there was no dose-dependent toxic effect. Thereby, we proposed that ALA has the potential for acting as photosensitivity insecticide for controlling O. chinensis. GSTs activity assays using CDNB and DCNB as substrates indicated that the thorax and abdomen of the different instar nymphs treated by ALA showed 1.52-5.56 fold significantly increased GSTs activities compared with the control. However, for the ESTs-specific activity assay, there was no significant difference between O. chinensis treated by ALA and the control within different instar nymphs, when a-NA, a-NB and b-NA were used as substrates. Therefore, GSTs-mediated metabolic detoxification as evidenced by significantly increased GSTs activities might contribute to protect against oxidative damage and oxidative stress by ALA in O. chinensis.

关键词： 5-aminolevulinic acid toxicity glutathione S-transferases general esterases Oxya chinensis

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Pharmacodynamic Mechanism of Kuanxiong Aerosol for Vasodilation and Improvement of Myocardial Ischemia

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Chinese Journal of Integrative Medicine 2022年第4期28卷 319-329页

作者： LU Yan yang mei-ling SHEN A-ling LIN Shan PENG mei-zhong WANG Tian-yi LU Zhu-qing WANG Yi-lian PENG Jun CHU Jian-feng Academy of Integrative Medicine Fujian University of Traditional Chinese MedicineFuzhou350122China Chen Keji Academic Thought Inheritance Studio Fujian University of Traditional Chinese MedicineFuzhou350122China Fujian Key Laboratory of Integrative Medicine on Geriatrics Fujian University of Traditional Chinese MedicineFuzhou350122China Department of Acupuncture and Moxibustion Third People’s Hospital Affiliated to Fujian University of Traditional Chinese MedicineFuzhou350122China

Objective: To explore the effect of Kuanxiong Aerosol(KXA)on isoproterenol(ISO)-induced myocardial injury in rat ***: Totally 24 rats were radomly divided into control,ISO,KXA low-dose and high-dose groups according to the randomized block design method,and were administered by intragastric administration for 10 consecutive days,and on the 9th and 10th days,rats were injected with ISO for 2 consecutive days to construct an acute myocardial ischemia model to evaluate the improvement of myocardial ischemia by *** addition,the diastolic effect of KXA on rat thoracic aorta and its regulation of ion channels were tested by in vitro vascular tension *** influence of KXA on the expression of calcium-CaM-dependent protein kinase Ⅱ(CaMK Ⅱ)/extracellular regulated protein kinases(ERK)signaling pathway has also been ***: KXA significantly reduced the ISO-induced increase in ST-segment,interventricular septal thickness,cardiac mass index and cardiac tissue pathological changes in ***,the relaxation of isolated thoracic arterial rings that had been precontracted using norepinephrine(NE)or potassium chloride(KCl)was increased after KXA treatment in an endothelium-independent manner,and was attenuated by preincubation with verapamil,but not with tetraethylammonium chloride,4-aminopyridine,glibenclamide,or barium *** pretreatment attenuated vasoconstriction induced by CaCl_(2)in Ca^(2+)-free solutions containing K^(+) or *** addition,KXA pretreatment inhibited accumulation of Ca^(2+)in A7r5 cells mediated by KCl and NE and significantly decreased p-CaMK Ⅱ and p-ERK ***: KXA may inhibit influx and release of calcium and activate the CaMK Ⅱ/ERK signaling pathway to produce vasodilatory effects,thereby improving myocardial injury.

关键词： Kuanxiong Aerosol myocardial ischemia vasodilation Ca^(2+) CaMKⅡ/ERK pathway

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On the relation between home numeracy activities and preschool children＇s numeracy development

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International English Education Research 2016年第2期 82-84页

作者： yang mei ling Foreign languages institute zhejiang university 310058

This mini-review focuses on the question of how home numeracy experience （i.e.,number-related activities at home） may affect a child＇s numeracy development. Evidence from young children suggests that trajectories of number learning differ for children having different home numeracy experiences. Children involved in higher frequency of direct numeracy practice tend to have higher numeracy skills, but the result becomes more complicated when considering the types of home numeracy activities. The relationship between the home numeracy experience and preschool children＇s nurneracy development may shed light on how children acquire number skills and develop their mathematics competence.

关键词： home numeracy experience preschool children numeracy development

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Characterization of Carboxylesterase Associated with Malathion Insensitivity in the Field Population of the Oriental Migratory Locust

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Agricultural Sciences in China 2008年第5期7卷 591-598页

作者： yang mei-ling ZHANG Jian-zhen ZHANG Jian-qin GUO Ya-ping MA En-bo Institute of Applied Biology Shanxi University Taiyuan 030006 P.R. China

Carboxylesterases （CarEs） from two field populations of the oriental migratory locust, Locusta migratoria manilensis （Meyen）, were examined to try to understand their contribution to malathion insensitivity. The CarEs activities in Wudi population （WD） were 1.75- and 1.50-fold significantly higher than those in Huangliu population （HL） when a-naphthyl acetate （a-NA） and [3-naphthyl acetate were used as substrates, respectively. Such elevated CarEs activities presented in the WD could be because of an increased staining intensity of the a-NA-hydrolyzing CarEs as shown on the nondenaturing polyacrylamide gel electrophoresis. Inhibition studies of CarEs using paraoxon and malaoxon indicated that CarE activities in the HL were more strongly inhibited than those in the WD. Furthermore, a 449-bp DNA fragment of CarE was obtained from L. migratoria manilensis. Hemiquantity reverse transcription-polymerase chain reaction analysis showed that CarE gene expression level in the WD was higher than that in the HL. The higher CarE activities and the increased CarE mRNA level in the WD appeared to be associated with decreased susceptibility to malathion in the WD due to the application of organophosphorus insecticides.

关键词： carboxylesterases enzyme kinetics enzyme inhibition RT-PCR Locusta migratoria manilensis

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Expression of Interleukin-6 and Interleukin-6 Receptor in Ovine Oocytes During In vitro Maturation

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Journal of Integrative Agriculture 2012年第8期11卷 1333-1339页

作者： ZHAO Xi-an CANG Ming GAO Xiao-yu yang mei-ling YUAN Jian-long ZHU Bing WANG Zhi-gang LIU Dong-jun Key Laboratory of Ministry of Education of China for Mammal Reproduction Biology and Biotechnology Inner Mongolia UniversityHohhot 010021P.R.China

To study the effects of interleukin-6 （IL-6） and its receptor （IL-6R） during in vitro maturation of ovine oocytes, the mRNA and protein expression levels of IL-6 and IL-6R, along with their localization, were examined during ovine oocytes maturation in vitro through real-time PCR, Western blotting, and immunohistochemistry. Specific patterns of expression of IL-6 and IL-6R were observed at both mRNA and protein levels at each stage of ovine oocytes maturation. IL-6 and IL-6R were distributed primarily on the surface of the cell membrane, with little expression in the cytoplasm or nucleus. IL-6 and IL-6R were expressed significantly at higher levels in the maturation around 4 h, and then decreased dramatically. However the level slightly elevated at 20-24 h. The role of IL-6 and IL-6R on oocytes maturation was studied through in vitro addition of recombinant human IL-6 in different concentrations. The addition of 10 ng mL-1 IL-6 significantly increased the rates of oocytes maturation （P〈0.05）, but did not affect the rates of development of the subsequence IVF ovine embryos. In summary, IL-6 is likely to play an important role in the early ovine oocytes maturation. The expression patterns of the IL-6 and IL-6R on the ovine oocytes maturation open up the possibility of regulatory role of the cytokine in ovine oocytes maturation.

关键词： IL-6 oocyte in vitro maturation real time PCR Western blotting

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Huoxin Pill(活心丸)Attenuates Cardiac Fibrosis by Suppressing TGF-β1/Smad2/3 Pathway in Isoproterenol-Induced Heart Failure Rats

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Chinese Journal of Integrative Medicine 2021年第6期27卷 424-431页

作者： PENG mei-zhong yang mei-ling SHEN A-ling ZHOU Xue-ling LU Yan LI Qi SHEN Zhi-qing HUANG Bin PENG Jun CHU Jian-feng Academy of Integrative Medicine Fujian University of Traditional Chinese MedicineFuzhou(350122)China Chen Keji Academic Thought Inheritance Studio Fujian University of Traditional Chinese MedicineFuzhou(350122)China Fujian Key Laboratory of Integrative Medicine on Geriatrics Fujian University of Traditional Chinese MedicineFuzhou(350122)China The Third People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine Fuzhou(350122)China Department of Research and Development Youcare Pharmaceutical Group Co.Ltd.Beijing(100176)China

Objective:To evaluate the effects of Huoxin Pill(活心丸,HXP)on cardiac fibrosis and heart failure(HF)in isoproterenol(ISO)-induced HF ***:Thirty Wistar rats were randomly divided into 5 groups including control,HF,isosorbide mononitrate(ISMN),HXP low(HXP-L),and HXP high(HXP-H)groups(n=6 for each group)according to the complete randomization *** were pretreated with ISMN(5 mg/kg daily),low concentration of HXP(10 mg/kg daily)or high concentration of HXP(30 mg/kg daily)or equal volume of saline by intragastric administration for 1 week,followed by intraperitoneal injection of ISO(10 mg/kg,14 days),and continually intragastric administrated with above medicines or saline for additional 6 *** effects of HXP treatment on the cardiac function,heart weight index(HWI),pathological changes,and collagen content were further ***,the role of HXP on activation of transforming growth factor-β1(TGF-β1)/Smads pathway was further explored using immunohistochemistry(IHC)and Westernblot ***:HXP treatment significantly alleviated the decrease of ejection fraction(EF)and fractional shortening(FS),while decreased the elevation of left ventricular end-systolic volume(LVESV)in ISO-induced HF rats(P<0.05).Moreover,HXP treatment obviously attenuated the increase of HWI and serum level of creatine kinase MB(CK-MB,P<0.05),as well as pathological changes in ISO-induced HF *** determination indicated that HXP treatment alleviated the elevation of collagenⅠand collagenⅢprotein expression in cardiac tissues of ISO-induced HF ***,HXP treatment significantly down-regulated the increase of TGF-β1 and p-Smad2/3 protein expression in cardiac tissues of HF rats(P<0.05),while did not affect the expression of total Smad2/***:HXP attenuated heart failure and cardiac fibrosis in ISO-induced HF rats by suppression of TGF-β1/Smad2/3 pathway.

关键词： Huoxin Pill heart failure cardiac fibrosis isoproterenol TGF-β1/Smad2/3 pathway

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高校图书馆读者借阅权限设定的实证分析

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图书馆论坛 2011年第3期31卷 50-53,122页

作者：杨玲梅华南农业大学图书馆广东广州510642

目前高校图书馆读者的借阅权限大多数都是按等级划分与同级平均分配的原则来设定的,然而读者需求量大小,各类文献的借阅周期不同才是决定读者借阅权限的重要因素。文章就上述两个因素进行定量分析,并与借阅权限作对比,针对存在的问题,... 详细信息

目前高校图书馆读者的借阅权限大多数都是按等级划分与同级平均分配的原则来设定的,然而读者需求量大小,各类文献的借阅周期不同才是决定读者借阅权限的重要因素。文章就上述两个因素进行定量分析,并与借阅权限作对比,针对存在的问题,提出合理设定高校图书馆读者借阅权限的办法,为充分开发与利用图书馆的文献资源提供参考。

关键词：读者需求高校图书馆借阅权限

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域名与商标权之冲突与协调

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中南民族大学学报（人文社会科学版） 2009年第4期29卷 113-117页

作者：杨玲梅汕头大学法学院广东汕头515063

域名是信息时代的产物,由于域名的特殊性质与技术特征,它与商标存在着密切的联系。因此,在商业活动中,不可避免地会出现域名与商标权相冲突的现象。当前,国际国内立法对域名与商标权冲突的协调还显得不足,应当通过完善该方面的立法来促... 详细信息

域名是信息时代的产物,由于域名的特殊性质与技术特征,它与商标存在着密切的联系。因此,在商业活动中,不可避免地会出现域名与商标权相冲突的现象。当前,国际国内立法对域名与商标权冲突的协调还显得不足,应当通过完善该方面的立法来促进我国知识经济的健康发展。

关键词：域名商标权知识经济

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单一和双重干扰源对混合型注意缺陷多动障碍儿童干扰控制的影响

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中国心理卫生杂志 2012年第5期26卷 345-349,401页

作者：杨美玲杨双苏州大学心理系江苏苏州215123

目的:探讨单一干扰源和双重干扰源对混合型注意缺陷多动障碍(ADHD)儿童的干扰控制的影响。方法:对25例符合美国精神障碍诊断与统计手册第4版(DSM-IV)诊断标准的门诊混合型ADHD儿童和25名性别、年龄和智力相匹配的对照组儿童,进行图形Str... 详细信息

目的:探讨单一干扰源和双重干扰源对混合型注意缺陷多动障碍(ADHD)儿童的干扰控制的影响。方法:对25例符合美国精神障碍诊断与统计手册第4版(DSM-IV)诊断标准的门诊混合型ADHD儿童和25名性别、年龄和智力相匹配的对照组儿童,进行图形Stroop测验,通过操纵图片的颜色和形状特征,设置了单一干扰和双重干扰两种条件,比较两组被试的冲突效应量。结果:在单一干扰条件下,ADHD组的冲突效应量显著大于对照组(P0.05)。结论:本研究提示,在单一干扰源条件下,混合型ADHD儿童表现出干扰控制缺陷,但在双重干扰源条件下,混合型ADHD儿童的干扰控制表现与正常儿童差异不显著。

关键词：注意缺陷多动障碍干扰控制 Stroop 试验性研究

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