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Identification of novel mammalian viruses in tree shrews(Tupaia belangeri chinensis)

Zoological Research 2024年第2期45卷 429-438页

作者： Hong Zhou Ren-Rong Tian Xiu-Rong Wang Jin-xuan yang yun-Xiao Wang Ming-liang Zhao Xu-Dong Zhang yu-Hua Ma long-Bao lv Edward CHolmes yong-Tang Zheng Wei-Feng Shi School of Clinical and Basic Medical Sciences Shandong First Medical University&Shandong Academy of Medical SciencesJi’nanShandong 250117China Key laboratory of Emerging Infectious Diseases in universities of Shandong Shandong First Medical University and Shandong Academy of Medical SciencesTaianShandong 271000China Key laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences/Key laboratory of Bioactive Peptides of yunnan Province KIZ-CUHK Joint Laboratory of Bioresources and Molecular Research in Common DiseasesCenter for Biosafety Mega-ScienceKunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan 650223China School of Public Health Shandong First Medical University&Shandong Academy of Medical SciencesJi’nanShandong 250117China National Resource Center for Non-Human Primates Kunming Primate Research CenterKunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan 650107China Sydney Institute for Infectious Diseases School of Medical SciencesThe University of SydneySydneyNew South Wales 2006Australia Department of Infectious Diseases Ruijin HospitalShanghai Jiao Tong University School of MedicineShanghai 200025China Shanghai Institute of Virology Shanghai Jiao Tong University School of MedicineShanghai 200025China

The Chinese tree shrew(Tupaia belangeri chinensis),a member of the mammalian order Scandentia,exhibits considerable similarities with primates,including humans,in aspects of its nervous,immune,and metabolic *** similarities have established the tree shrew as a promising experimental model for biomedical research on cancer,infectious diseases,metabolic disorders,and mental health ***,we used metatranscriptomic sequencing to analyze plasma,as well as oral and anal swab samples,from 105 healthy asymptomatic tree shrews to identify the presence of potential zoonotic *** total,eight mammalian viruses with complete genomes were identified,belonging to six viral families,including Flaviviridae,Hepeviridae,Parvovirinae,Picornaviridae,Sedoreoviridae,and ***,the presence of rotavirus was recorded in tree shrews for the first *** viruses-hepacivirus 1,parvovirus,and picornavirus-exhibited low genetic similarity(usly reported viruses at the whole-genome scale,indicating ***,three other viruses-hepacivirus 2,hepatovirus A and hepevirus-exhibited high similarity(>94%)to known viral *** analyses also revealed that the rotavirus and mammalian orthoreovirus identified in this study may be novel *** findings provide insights into the diverse viral spectrum present in captive Chinese tree shrews,highlighting the necessity for further research into their potential for crossspecies transmission.

关键词： Tree shrew(Tupaia belangeri chinensis) Meta-transcriptomic sequencing Mammalian viruses Genomic analysis

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High formability Mg-Zn-Gd wire facilitates ACl reconstruction via its swift degradation to accelerate intra-tunnel endochondral ossification

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Journal of Magnesium and Alloys 2024年第1期12卷 295-315页

作者： xuan He ye li Hongwei Miao Jiankun Xu Michael Tim-yun Ong Chenmin Wang lizhen Zheng Jiali Wang le Huang Haiyue Zu Zhi yao Jie Mi Bingyang Dai Xu li Patrick Shu-hang yung Guangyin yuan ling Qin Musculoskeletal Research laboratory of Department of Orthopaedics&Traumatology and Innovative Orthopaedic Biomaterial and Drug Translational Research laboratory of li Ka Shing Institute of Health Sciences The Chinese University of Hong KongHong KongChina National Engineering Research Center of light Alloy Net Forming School of Materials Science&EngineeringShanghai Jiao Tong UniversityShanghaiChina Department of Orthopaedics and Traumatology Li Ka Shing Faculty of MedicineThe University of Hong KongHong KongChina Orthopaedic Department Peking University Third HospitalBeijingChina Department of Rehabilitation Sciences The Hong Kong Polytechnic UniversityHong KongChina

After reconstructing the anterior cruciate ligament(ACl),unsatisfactory bone tendon interface healing may often induce tunnel enlargement at the early healing *** good biological features and high formability,Magnesium-Zinc-Gadolinium(ZG21)wires are developed to bunch the tendon graft for matching the bone tunnel during ***,tensile strength,degradation,and cytotoxicity of ZG21 wire are *** rabbit model is used for assessing the biological effects of ZG21 wire by Micro-CT,histology,and mechanical *** SEM/EDS,immunochemistry,and in vitro assessments are performed to investigate the underlying *** tests demonstrate the high formability of ZG21 wire as surgical ***-CT shows ZG21 wire degradation accelerates tunnel bone formation,and histologically with earlier and more fibrocartilage regeneration at the healing *** mechanical test shows higher ultimate load in the ZG21 *** SEM/EDS presents ZG21 wire degradation triggered calcium phosphate(Ca-P)*** results demonstrate upregulation of Wnt3a,BMP2,and VEGF at the early phase and TGFβ3 and Type II collagen at the late phase of *** vitro tests also confirmed the Ca-P in the metal extract could elevate the expression of Wnt3a,βcatenin,ocn and opn to stimulate *** vivo tests of clinical samples indicated suturing with ZG21 wire did not weaken the ultimate loading of human tendon *** conclusion,the ZG21 wire is feasible for tendon graft *** degradation products accelerated intra-tunnel endochondral ossification at the early healing stage and therefore enhanced bone-tendon interface healing in ACl reconstruction.

关键词： Magnesium wire ACl reconstruction Magnesium alloy Biomaterials Endochondral ossification

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Determination of the number ofψ(3686)events taken at BESⅢ

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Chinese Physics C 2024年第9期48卷 8-20页

作者： M.Ablikim M.N.Achasov P.Adlarson O.Afedulidis X.C.Ai R.Aliberti A.Amoroso Q.An y.Bai O.Bakina I.Balossino y.Ban H.-R.Bao V.Batozskaya K.Begzsuren N.Berger M.Berlowski M.Bertani D.Bettoni F.Bianchi E.Bianco A.Bortone I.Boyko R.A.Briere A.Brueggemann H.Cai X.Cai A.Calcaterra G.F.Cao N.Cao S.A.Cetin J.F.Chang G.R.Che G.Chelkov C.Chen C.H.Chen Chao Chen G.Chen H.S.Chen H.y.Chen M.l.Chen S.J.Chen S.l.Chen S.M.Chen T.Chen X.R.Chen X.T.Chen y.B.Chen y.Q.Chen Z.J.Chen Z.y.Chen S.K.Choi G.Cibinetto F.Cossio J.J.Cui H.l.Dai J.P.Dai A.Dbeyssi R.E.de Boer D.Dedovich C.Q.Deng Z.y.Deng A.Denig I.Denysenko M.Destefanis F.De Mori B.Ding X.X.Ding y.Ding y.Ding J.Dong l.y.Dong M.y.Dong X.Dong M.C.Du S.X.Du y.y.Duan Z.H.Duan P.Egorov y.H.Fan J.Fang J.Fang S.S.Fang W.X.Fang y.Fang y.Q.Fang R.Farinelli l.Fava F.Feldbauer G.Felici C.Q.Feng J.H.Feng y.T.Feng M.Fritsch C.D.Fu J.l.Fu y.W.Fu H.Gao X.B.Gao y.N.Gao yang Gao S.Garbolino I.Garzia l.Ge P.T.Ge Z.W.Ge C.Geng E.M.Gersabeck A.Gilman K.Goetzen l.Gong W.X.Gong W.Gradl S.Gramigna M.Greco M.H.Gu y.T.Gu C.y.Guan Z.l.Guan A.Q.Guo l.B.Guo M.J.Guo R.P.Guo y.P.Guo A.Guskov J.Gutierrez K.l.Han T.T.Han F.Hanisch X.Q.Hao F.A.Harris K.K.He K.l.He F.H.Heinsius C.H.Heinz y.K.Heng C.Herold T.Holtmann P.C.Hong G.y.Hou X.T.Hou y.R.Hou Z.l.Hou B.y.Hu H.M.Hu J.F.Hu S.l.Hu T.Hu y.Hu G.S.Huang K.X.Huang l.Q.Huang X.T.Huang y.P.Huang T.Hussain F.Hölzken N.Hüsken N.in der Wiesche J.Jackson S.Janchiv J.H.Jeong Q.Ji Q.P.Ji W.Ji X.B.Ji X.l.Ji y.y.Ji X.Q.Jia Z.K.Jia D.Jiang H.B.Jiang P.C.Jiang S.S.Jiang T.J.Jiang X.S.Jiang y.Jiang J.B.Jiao J.K.Jiao Z.Jiao S.Jin y.Jin M.Q.Jing X.M.Jing T.Johansson S.Kabana N.Kalantar-Nayestanaki X.l.Kang X.S.Kang M.Kavatsyuk B.C.Ke V.Khachatryan A.Khoukaz R.Kiuchi O.B.Kol.u B.Kopf M.Kuessner X.Kui N.Kumar A.Kupsc W.Kühn J.J.lane P.larin l.lavezzi T.T.lei Z.H.lei M.lellmann T.lenz C.li C.li C.H.li Cheng li D.M.li F.li G.li H.B.li H.J.li H.N.li Hui li J.R.li J.S.li Ke li l.J.li l.K.li lei li M.H.li P.R.li Q.M.li Q.X.li R.li S.X.li T.li W.D.li W.G.li X.li X.H.li X.l.li X.Z.li Xi Institute of High Energy Physics Beijing 100049China Beihang university Beijing 100191China Bochum Ruhr-university D-44780BochumGermany Budker Institute of Nuclear Physics SB RAS(BINP) Novosibirsk 630090Russia Carnegie Mellon university PittsburghPennsylvania 15213USA Central China Normal.university Wuhan 430079China Central South university Changsha 410083China China Center of Advanced Science and Technology Beijing 100190China China university of Geosciences Wuhan 430074China Chung-Ang university Seoul06974Republic of Korea COMSATS university Islamabad Lahore CampusDefence RoadOff Raiwind Road54000LahorePakistan Fudan university Shanghai 200433China GSI Helmholtzcentre for Heavy Ion Research GmbH D-64291DarmstadtGermany Guangxi Normal.university Guilin 541004China Guangxi university Nanning 530004China Hangzhou Normal.university Hangzhou 310036China Hebei university Baoding 071002China Helmholtz Institute Mainz Staudinger Weg 18D-55099MainzGermany Henan Normal.university Xinxiang 453007China Henan university Kaifeng 475004China Henan university of Science and Technology Luoyang 471003China Henan university of Technology Zhengzhou 450001China Huangshan College Huangshan 245000China Hunan Normal.university Changsha 410081China Hunan university Changsha 410082China Indian Institute of Technology Madras Chennai 600036India Indiana university BloomingtonIndiana 47405USA INFN laboratori Nazionali di Frascati (A)INFN Laboratori Nazionali di FrascatiI-00044FrascatiItaly(B)INFN Sezione di PerugiaI-06100PerugiaItaly(C)University of PerugiaI-06100PerugiaItaly INFN Sezione di Ferrara (A)INFN Sezione di FerraraI-44122FerraraItaly(B)University of FerraraI-44122FerraraItaly Inner Mongolia university Hohhot 010021China Institute of Modern Physics Lanzhou 730000China Institute of Physics and Technology Peace Avenue 54BUlaanbaatar 13330Mongolia Instituto de Alta Investigación Universidad de TarapacáCasilla 7DArica 1000000Chile Jilin university Changchun 130012China Joha

The number ofψ(3686)events collected by the BESⅢdetector during the 2021 run period is determined to be(2259.3±11.1)×10~6 by counting inclusiveψ(3686)hadronic *** uncertainty is systematic and the statistical.uncertainty is ***,the numbers ofψ(3686)events collected during the 2009 and 2012run periods are updated to be(107.7±0.6)×10~6 and(345.4±2.6)×10~6,*** numbers are consistent with the previous measurements within one standard *** total number ofψ(3686)events in the three data samples is(2712.4±14.3)×10~6.

关键词： ψ(3686) inclusive process Hadronic events BESⅢdetector

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u型组合再生混凝土-高强混凝土梁抗火性能

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工程力学 2024年第6期41卷 9-18页

作者：肖建庄陈子璇同济大学土木工程学院上海200092 广西大学土木建筑工程学院南宁530004 广西大学双碳科学与技术研究院南宁530004

为突破单一混凝土材料在受力和抗火两方面的性能难以协同优化的难题,该文创新性地提出u型组合再生混凝土-高强混凝土梁(简称为RAC-HSC组合梁),系统地开展了火灾试验和常温、火后受弯试验,综合分析了不同工况下组合梁的性能表现。研究表... 详细信息

为突破单一混凝土材料在受力和抗火两方面的性能难以协同优化的难题,该文创新性地提出u型组合再生混凝土-高强混凝土梁(简称为RAC-HSC组合梁),系统地开展了火灾试验和常温、火后受弯试验,综合分析了不同工况下组合梁的性能表现。研究表明:火灾下,RAC-HSC组合梁的耐火性能显著优于高强混凝土整浇梁,其梁内温度较低,跨中挠度较小,跨中未出现明显裂缝,且未观察到爆裂现象;常温及高温后的受弯试验中,RAC-HSC组合梁的屈服荷载、极限承载力及初始刚度均显著优于再生混凝土梁,基本达到或超过普通混凝土整浇梁和高强混凝土整浇梁的水平。该文所提出的u型组合再生混凝土-高强混凝土梁是混凝土构件“抗火-受力一体化设计”的有益尝试。

关键词：组合混凝土梁再生混凝土高强混凝土抗火性能受力性能

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基于国产商用ClBO晶体的高转换效率、高功率深紫外266 nm激光

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中国激光 2024年第7期51卷 234-240页

作者：俞航航张志韬玄洪文广东大湾区空天信息研究院广东广州510700 广东省太赫兹量子电磁学重点实验室广东广州510700 中国科学院大学北京100049

深紫外激光具有光子能量高、波长短等特点,在激光加工、半导体光刻等领域中具有重要的应用价值。固体激光非线性频率变换是实现高功率、高相干性深紫外激光输出的主要方式之一。采用全固态532 nm激光作为基频光、国产商用CsliB_(6)O_(10... 详细信息

深紫外激光具有光子能量高、波长短等特点,在激光加工、半导体光刻等领域中具有重要的应用价值。固体激光非线性频率变换是实现高功率、高相干性深紫外激光输出的主要方式之一。采用全固态532 nm激光作为基频光、国产商用CsliB_(6)O_(10)(ClBO)晶体作为频率变换晶体,在基频光功率为34.2 W时,实现了平均功率为14 W、重复频率为100 kHz、脉冲宽度为1.8 ns的266 nm深紫外激光输出,光-光转换效率达到41%。该深紫外光源具有效率高、结构紧凑的优点,验证了国产商用ClBO晶体的实用性,可进一步获得更稳定、更高功率的深紫外激光输出。

关键词：激光器深紫外激光全固态激光 CsliB_(6)O_(10)晶体高功率

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Efficient and selective removal of Pb(Ⅱ) from landfill leachate using l-serine-modified polyethylene/polypropylene nonwoven fabric synthesized via radiation grafting technique

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Nuclear Science and Techniques 2024年第4期35卷 144-155页

作者： Xin-Xin Feng Cheng li xuan-Zhi Mao Wan-Ning Ren yang Gao yu-long He Zhe Xing Rong li Guo-Zhong Wu Shanghai Institute of Applied Physics Chinese Academy of SciencesShanghai 201800China university of Chinese Academy of Sciences Beijing 100049China School of Physical Science and Technology ShanghaiTech UniversityShanghai 201210China

In this study,to efficiently remove Pb(Ⅱ) from aqueous environments,a novel l-serine-modified polyethylene/polypropylene nonwoven fabric sorbent(NWF-serine)was fabricated through the radiation grafting of glycidyl methacrylate and subsequent l-serine *** effect of the absorbed dose was investigated in the range of 5–50 ***-serine was characterized by Fourier transform infrared spectroscopy,thermogravimetric analysis,and scanning electron *** adsorption tests were conducted to investigate the influences of pH,adsorption time,temperature,initial concentration,and sorbent dosage on the Pb(Ⅱ) adsorption performance of *** results indicated that Pb(Ⅱ) adsorption onto NWF-serine was an endothermic process,following the pseudo-second-order kinetic model and langmuir isotherm *** saturated adsorption capacity was 198.1 mg/***-serine exhibited Pb(Ⅱ) removal rates of 99.8% for aqueous solutions with initial concentrations of 100 mg/l and 82.1% for landfill leachate containing competitive metal ions such as Cd,Cu,Ni,Mn,and ***,NWF-serine maintained 86% of its Pb(Ⅱ) uptake after five use *** coordination of the carboxyl and amino groups with Pb(Ⅱ) was confirmed using X-ray photoelectron spectroscopy and extended X-ray absorption fine structure analysis.

关键词： landfill leachate Radiation grafting Polyethylene/polypropylene nonwoven fabric Pb(Ⅱ)removal

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Experimental Study of a Solar Thermal Incubator using a Phase-Change Material for Off-Grid use

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Open Journal of Applied Sciences 2024年第9期14卷 2471-2481页

作者： yéouélé Thio Bati Ernest Boya Bi Ekoun Paul Magloire Koffi Prosper Gbaha laboratoire des Procds Industriels de Synthse de lEnvironnement et des Energies Nouvelles Institut National Polytechnique Flix Houphout-Boigny Yamoussoukro Cte dIvoire

The aim of this study was to provide a simple, easy-to-use incubation system for small-scale rural poultry farmers far from the electricity grid. To this end, a naturally ventilated solar thermal incubator was built and experimentally tested. A u-shaped evacuated tube collector and a wooden crate holding 50 eggs were used to build the solar thermal incubator. Water was used as the heat transfer fluid, and an EPCM was integrated into the incubation chamber for operation at night or when the sun was hidden. The heat generated by the solar collector and stored in the heat transfer fluid is transported to the incubation chamber by thermosiphon to heat the chamber. Temperature and humidity probes powered by a solar panel were placed at various locations to monitor the thermo-hygrometric efficiency of the incubation system. The incubator, heated by natural convection, proved to function normally, and the incubation chamber was maintained throughout the incubation period within a temperature range of 35.53˚C to 39.53˚C and relative humidity averaging 49.4% to 68.5%. The experiment was carried out by introducing 30 eggs and the results of the experimental study showed that the incubator’s efficiency was 87%. The performance tests gave a fertility rate of 93% and a hatching rate of 93%, i.e. 28 fertile eggs and 26 hatched eggs, respectively.

关键词： Solar Incubator Vacuum Tube Collector Encapsulated PCM Poultry Eggs

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lncRNA CCRR maintains Ca^(2+)homeostasis against myocardial infarction through the FTO-SERCA2a pathway

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Science China(life Sciences) 2024年第8期67卷 1601-1619页

作者： Hua yang lina xuan Shengjie Wang Huishan luo Xiaomeng Duan Jianjun Guo Shijia Cui Jieru Xin Junwei Hao Xiufang li Jun Chen Feihan Sun Xiaolin Hu Siyun li ying Zhang lei Jiao Baofeng yang lihua Sun Department of Pharmacology Harbin Medical University(State Key Laboratory of Frigid Zone Cardiovascular Diseasethe State-Province Key Laboratories of Biomedicine-Pharmaceutics of ChinaKey Laboratory of Cardiovascular ResearchMinistry of EducationJoint International Research Laboratory of Cardiovascular Medicine ResearchMinistry of EducationChina)College of PharmacyHarbin Medical UniversityHarbin 150081China

Cardiac conduction regulatory RNA(CCRR)has been documented as an antiarrhythmic lncRNA in our earlier *** study aimed to evaluate the effects of CCRR on SERCA2a and the associated Ca^(2+)homeostasis in myocardial infarction(MI).Overexpression of CCRR via AAV9-mediated delivery not only partially reversed ischemia-induced contractile dysfunction but also alleviated abnormal Ca^(2+)homeostasis and reduced the heightened methylation level of SERCA2a following *** effects were also observed in CCRR overexpressing transgenic mice.A conserved sequence domain of CCRR mimicked the protective function observed with the full ***,silencing CCRR in healthy mice led to intracel.ular Ca^(2+)overloading of *** increased SERCA2a protein stability by upregulating FTO *** direct interaction between CCRR and FTO protein was characterized by RNA-binding protein immunoprecipitation(RIP)analysis and RNA pulldown *** of NFATc3 was identified as an upstream mechanism responsible for CCRR downregulation in *** study demonstrates that CCRR is a protective lncRNA that acts by maintaining the function of FTO,thereby reducing the m^(6)A RNA methylation level of SERCA2a,ultimately preserving calcium homeostasis for myocardial contractile function in ***,CCRR may be considered a promising therapeutic strategy with a beneficial role in cardiac pathology.

关键词： CCRR calcium homeostasis FTO myocardial infarction SERCA2a

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基于稀土上转换纳米粒子传感器的构建及其在食品添加剂检测中的应用

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光谱学与光谱分析 2024年第9期44卷 2459-2466页

作者：张璇王雅森温娜吕海霞李宝铭福州大学材料科学与工程学院分子材料与工程系福建福州350108

稀土上转换纳米粒子(uCNPs)因其具有毒性小、化学稳定性好和背景荧光低等优点被用于构建荧光纳米传感器。通过溶剂热法制备核壳uCNPs,采用柠檬酸钠对其进行表面配体交换得到水溶性核壳uCNPs(Cit-CS-uCNPs),以Cit-CS-uCNPs作为荧光传感... 详细信息

稀土上转换纳米粒子(uCNPs)因其具有毒性小、化学稳定性好和背景荧光低等优点被用于构建荧光纳米传感器。通过溶剂热法制备核壳uCNPs,采用柠檬酸钠对其进行表面配体交换得到水溶性核壳uCNPs(Cit-CS-uCNPs),以Cit-CS-uCNPs作为荧光传感器的能量供体,二氧化锰(MnO_(2))纳米片作为荧光传感器的能量受体,采用荧光共振能量转移(FRET)机理,构建了一种用于食品添加剂二氧化氢(H_(2)O_(2))和叔丁基对苯二酚(TBHQ)检测的荧光纳米传感器(Cit-CS-uCNPs-MnO_(2))。通过扫描电镜(SEM)、荧光光谱和紫外可见吸收光谱(uV-Vis)等分析测试手段对制备的纳米材料的形貌结构和性能进行表征。考察了猝灭剂浓度、孵育温度及孵育时间等实验条件对荧光传感体系检测性能的影响。荧光光谱和uV-Vis实验结果表明,Cit-CS-uCNPs最大发射峰位于654nm,Cit-CS-uCNPs与MnO_(2)结合后,核壳uCNPs发生荧光猝灭,当存在H_(2)O_(2)时,Cit-CS-uCNPs的荧光恢复,说明在此波段H_(2)O_(2)与MnO_(2)纳米片发生氧化还原反应,MnO_(2)纳米片被还原成Mn^(2+),逐渐从Cit-CS-uCNPs的表面解离下来;存在TBHQ时,Cit-CS-uCNPs-MnO_(2)与TBHQ体系峰位与TBHQ偏移到253nm,为TBHQ与MnO_(2)纳米片发生氧化还原反应,使得Cit-CS-uCNPs-MnO_(2)体系中FRET效应减弱,荧光强度回升。SEM结果表明,MnO_(2)纳米片均匀包覆在Cit-CS-uCNPs的周围,在水中仍可保持良好的分散性,表明MnO_(2)纳米片修饰到Cit-CS-uCNPs表面。对猝灭剂高锰酸钾(KMnO4)的浓度进行了优化。结果表明,当KMnO4浓度为10mol·l^(-1)时,猝灭效率可达90%。对检测条件进行优化,结果表明H_(2)O_(2)的检测孵育时间为25min时,MnO_(2)和H_(2)O_(2)之间氧化还原反应基本完全,Cit-CS-uCNPs-MnO_(2)荧光恢复值最大;TBHQ的检测孵育时间为30min。在最佳实验条件下,Cit-CS-uCNPs-MnO_(2)的荧光强度变化比率与H_(2)O_(2)浓度(0~1000μmol·l^(-1))及TBHQ浓度(0~0.6 mmol·l^(-1))分别具有良好的线性关系。保持最佳实验条件,选取食品中存在的代表性金属离子(如K+、Na+、Ca^(2+)和Mg^(2+))和常见的食品添加剂苯甲酸(BA)、D-葡萄糖(Glu)、山梨酸钾(PS)、蔗糖(Suc)、纳他霉素(Nat)和肌醇(Ino)作为研究对象进行选择性测试,发现与H_(2)O_(2)相比,Cit-CS-uCNPs-MnO_(2)对其他添加物质没有强烈的反应,传感器整体荧光信号波动不大,因此Cit-CS-uCNPs-MnO_(2)对H_(2)O_(2)和TBHQ可进行特异性检测。

关键词：上转换纳米粒子核壳结构荧光纳米传感器

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基于α-葡萄糖苷酶抑制活性评价青稞全粉提取物抑制效果及其相互作用

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粮油食品科技 2024年第3期32卷 132-139页

作者：任欣刘璇王补珍张敏王林萱北京工商大学食品与健康学院国家粮食产业(青稞深加工)技术创新中心北京市食品添加剂工程技术研究中心北京100048

青稞是我国青藏高原地区特有的粮食作物,具有良好的改善血糖代谢功效,但其具体降糖活性成分以及它们之间的配伍关系尚未明确。以青稞全粉为研究对象,提取青稞全粉中的多酚、多糖、多肽三种关键活性成分,探究青稞全粉提取物对α-葡萄糖... 详细信息

青稞是我国青藏高原地区特有的粮食作物,具有良好的改善血糖代谢功效,但其具体降糖活性成分以及它们之间的配伍关系尚未明确。以青稞全粉为研究对象,提取青稞全粉中的多酚、多糖、多肽三种关键活性成分,探究青稞全粉提取物对α-葡萄糖苷酶的抑制能力及其相互作用。通过单因素实验以及Box-Benhnken响应面优化实验,确定了利用微孔板法测定α-葡萄糖苷酶抑制活性的最佳反应体系,即酶浓度0.5 u/ml、缓冲液浓度80 mmol/l、底物浓度5 mmol/l。三种青稞全粉提取物均具有一定的α-葡萄糖苷酶抑制活性,其中青稞多酚的抑制活性最强,α-葡萄糖苷酶活性抑制率达到83.63%±2.92%,对应的IC_(50)值为0.18 mg/ml。三种青稞全粉提取物两两复配均具有一定的协同增效作用。结果可为血糖控制人群的膳食选择和功能性食品的复配开发提供理论指导。

关键词：青稞全粉活性组分 α-葡萄糖苷酶抑制活性协同配伍

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