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On abiogenic natural gas

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Chinese Science Bulletin 1997年第16期42卷 1327-1337页

作者： Wang, XB Li, CY Chen, JF Xia, xy Guo, ZQ Xie, HS Chinese Acad Sci Lanzhou Inst Geol Lanzhou 730000 Peoples R China Daqing Petr Explorat Bur Daqing Inst Petr Explorat & Dev Daqing 163000 Peoples R China Chinese Acad Sci Inst Geochem Guiyang 550002 Peoples R China

NATURAL gases usually refer to the inflammable gases dominated by methane from the angle of research on energy gases. Three genetic types of natural gases can be recognized according to the process of methane generation: (ⅰ) biogenic (bacterial) gas, mainly the gas formed by the decomposition of organic matter in sediments under the action of anaerobic bacteria; (ⅱ) thermogenic gas, mainly the gas formed by the degradation of organic matter in sediments under the action of certain temperature and pressure; (ⅲ) abiogenic gas, mainly the

关键词： abiogenic natural gas.

Polishing technology of surface for diamond film using the method of laser ablation

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Chinese Science Bulletin 1997年第23期42卷 2010-2013页

作者： Gu, CZ Jin, ZS Wang, YG Zhuang, RS Lu, xy Zhao, ST Zou, GT Jilin Univ State Key Lab Superhard Mat Changchun 130023 Peoples R China

THE chemical vapor deposition (CVD) methods have been developed so much in the past tenyears that it has become less expensive to deposit a thick CVD diamond film than to polish thediamond. The polycrystalline films nomnally exhibit a pronounced roughness because there arelots of large pointed grains on the surface of the films. On the other hand, the thickness unityof diamond film is poor because of the effects of deposition condition, especially for those syn-thesized by the methods of hot filament CVD (HFCVD) and electron assistant CVD(EACVD). Poor thickness unity and rough surface of thick diamond film limits their poten-

关键词： diamond film laser ablation polishing of surface.

A clinical evaluation of serological diagnosis for pancreatic cancer

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World Journal of Gastroenterology 1998年第2期4卷 55-57页

作者： Zhao, xy Yu, SY Da, SP Bai, L Guo, XZ Dai, XJ Wang, YM Third Mil Med Univ Xinqiao Hosp Dept Gastroenterol Chongqing 400037 Peoples R China

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ＡｃｌｉｎｉｃａｌｅｖａｌｕａｔｉｏｎｏｆｓｅｒｏｌｏｇｉｃａｌｄｉａｇｎｏｓｉｓｆｏｒｐａｎｃｒｅａｔｉｃｃａｎｃｅｒＺＨＡＯＸｉａｏＹａｎ，ＹＵＳｈｉＹｕａｎ，ＤＡＳｈｉＰｉｎｇ，ＢＡＩＬｉ，ＧＵＯＸｉａｏＺｈｏｎｇ，ＤＡＩＸｉａｏＪ...

关键词： pancreatic neoplasms/diagnosis tumor markers, biological antigens, neoplasm/analysis CA 19 9 antigen/analysis pancreatopeptidase/analysis carcinoembryonic antigen/analysis alpha 1 antitrypsin/analysis enzyme linked immunosorbent assay radioim

Construction of human combinatorial antibody library and screening of monoclonal antibody Fabs to human immunodeficiency virus type Ⅰ

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Chinese Science Bulletin 1999年第4期44卷 352-356页

作者： Wang, X He, YX Zhou, Y Liu, S Yan, xy Tien, P Chinese Acad Sci Inst Microbiol Beijing 100080 Peoples R China Beijing Ditan Hosp Dept Virol Beijing 100011 Peoples R China

The heavy chain Fd genes and K chain genes of immunoglobulin were amplified by RT-PCR from PBL of three volunteer donors with HIV-positive. Phage antibody library was constructed with the Fd genes and K chain genes using pComb3 as vector. Three-round selection against coated gpl20 showed specific enrichment of phage antibodies. After the third round selection, 40 out of 50 clones exhibited gpl20 binding capacity. The specificity of the clones was verified by ELISA and competition inhibition ELISA. The V_H was derived from subgroups V_Ⅱ and V_Ⅲ, V_L belonged to subgroups V_K Ⅰ and V_K Ⅲ with DNA sequencing. These results suggest that the antibodies obtained are specific to gpi20.

关键词： immunoglobulin combinatorial library phage antibody Fab gp120 HIV-1.

Spectroscopic studies on interaction of hemoglobin and serum albumin with nicotine

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Chinese Science Bulletin 2002年第7期47卷 538-542页

作者： Wang, HF Wang, Y Cheng, Y Sun, HF Wang, xy Liu, YF Peking Univ Coll Chem & Mol Engn Dept Appl Chem Beijing 100871 Peoples R China

The interactions of nicotine and Hb/SA were studied in vitro by UV/Vis, fluorescence, 1H NMR and FT-IR spectroscopies. The UV/Vis absorbance of Hb/SA (200nm) shifted to red and decreased gradually with the addition of nicotine, indicating that the protein conformational change resulted from the chemical interaction. With increasing nicotine concentration, incubation of SA with nicotine caused the quenching of fluorescence typical of protein tryptophan residues, which meant that the vicinity of the tryptophan residues of SA was changed because of nicotine. FT-IR spectra showed that α-helix component of Hb/SA decreased, turn and β-structure components of Hb/SA increased in the presence of nicotine. In the 1H NMR spectra of nicotine, all proton peaks on pyrrolidinyl ring moved to downfield and the resonance emanating from nicotine was preferentially broadened while the concentration of Hb/SA increased. All these results indicate that nicotine and Hb/SA in vitro interact on each other, forming a new

关键词： hemoglobin (Hb) serum albumin (SA) nicotine spec-troscopic studies.

THE SYNTHESIS AND 2D NMR STUDY OF DELTORPHINS

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Chinese Chemical Letters 1995年第4期6卷 285-288页

作者： Wang, R Hu, xy Ji, H Xu, XJ Zhang, RH Lai, LH Lanzhou Univ Dept Biol Natl Lab Appl Organ Chem Lanzhou 730000 Peoples R China Beijing Univ Dept Chem Beijing 100831 Peoples R China

We synthesized Deltorphin I, Deltorphin II, and their L-Alanine analogs at position 2. The synthesis was carried out with SPPS and Boc strategy. The data, we got from FAB MS, AAA and sequence analysis were in agreement with the calculated values. We studied the 2D NMR for Deltorphin II and [L-Ala(2)]Deltorphin II in DMSO solution. The H-1-H-1 COSY and 2D NOESY spectra were resolved by computer treatment. The conformations of C-fragment tetrapeptides of Deltorphin II and its L-Ala analog might form a 3(10) alpha -helix. The molecular models of these two peptides were established.

关键词： NMR THE SYNTHESIS AND 2D NMR STUDY OF DELTORPHINS

Efficacies of β-L-D4A against Hepatitis B virus in 2.2.15 cells

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World Journal of Gastroenterology 2008年第8期14卷 1263-1267页

作者： Lin-Lin Gao, Xiao-Yan Wang, Ju-Sheng Lin, Ying-Hui Zhang, Yan Li, Institute of Liver Diseases, Tongji Hospital, Tongii Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China Author contributions:Gao LL and Wang xy contributed equally to this work Gao LL, Wang xy, Lin JS, Zhang YH, Li Y designed the research Gao LL and Wang xy performed the research Gao LL wrote the paper Institute of Liver Diseases Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 Hubei Province China.

AIM： To investigate the antiviral effect of beta-L- enantiomer of 2;3＇-didehydro-2＇,3″-dideoxyadenosine （13-L-D4A） on 2.2.15 cells transfected with the hepatitis B virus （HBV） genome.METHODS： Lamivudine （3TC） as a positive control. Then, HBV DNA in treated 2.2.15 cells and the Hepatitis B surface antigen （HBsAg） in the culture supernatants were detected to determine the inhibitory effect of β-L- D4A. At the same time, 3-（4,5-dimethylthiazol-2-yl）-2,5- diphenyltetrazolium bromide （MTT） was used to detect the survival ratio of 2.2.15 cells.RESULTS： β-L-D4A has a dose-dependent inhibitory effect on HBV DNA replication; this effect was apparent when the concentration was above 1 mol/L. When β-L- D4A was at the highest concentration, 100 mol/L, the HBsAg inhibition ratio was above 50%. The Therapeutic index （TI） of β-L-D4A was above 2.1.CONCLUSION： β-L-D4A has a dose-dependent inhibitory effect on the replication of HBV DNA and the secretion of HBsAg at low toxicity,

关键词： 2 3'-didehydro-2 3'-dideoxyadenosine 2.2.15 cells Hepatitis B virus DNA Hepatitis B surface antigen

Current status of radiology in China

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World Journal of Gastroenterology 2000年第2期6卷 193-197页

作者： Zeng SM Zhou KR Zhang ZY Peng WJ Yang FH Lin J Yang J Han xy Shanghai Med Univ Zhongshan Hosp Dept Radiol Shanghai 200032 Peoples R China

INTRODUCTIONRadiology has been greatly advanced in China sinceits founding in 1949 and has been developed fasterand further more since China adopted the policy ofsocioeconomic reform in *** plays anincreasingly important role in the medical healthcare and treatment in the country and has reachedthe world’s advanced level in certain *** nowbriefly review the history of China’s radiology so asto give a clear picture of its development.

关键词： radiology/history radiology/leducation radiology/trends China

The Effect of Cd on the Electrochromic Properties of NiO_xH_y film

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Chinese Chemical Letters 1998年第5期9卷 473-476页

作者： Cao, xy Zhang, WL Zhang, GY Zhang, WH Lin, YN Yuan, HT Zhou, ZX Zhang, YS Nankai Univ Inst New Energy Mat Chem Tianjin 300071 Peoples R China Nankai Univ Photon Ctr Tianjin 300071 Peoples R China

The effects of 2.8% Cd on the electrochromic properties of NiOxHy film were studied. The film was electrodeposited on both the polished Ni plate and conductive ITO glass to study its electrochemical and optical characteristics respectively. It was found that Cd could decrease the working voltage and increase the oxygen evolution voltage as well as the transmittance difference of colored and breached states of the film. However, it has little effect on the kinetic process.

关键词： NiOxHy film Cd electrochromic properties