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Lineage tracing reveals the origin of Nestin-positive cells are heterogeneous and rarely from ependymal cells after spinal cord injury

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Science China(Life Sciences) 2022年第4期65卷 757-769页

作者： Xiaoyu xue Muya Shu Zhifeng Xiao Yannan Zhao Xing Li Haipeng Zhang Yongheng Fan Xianming wu Bing Chen Bai Xu Yaming Yang Weiyuan Liu Sumei Liu Jianwu Dai State Key Laboratory of Molecular Developmental Biology Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing100190China University of Chinese Academy of Sciences Beijing100190China

Nestin is expressed extensively in neural stem/progenitor cells during neural development, but its expression is mainly restricted to the ependymal cells in the adult spinal cord. After spinal cord injury(SCI), Nestin expression is reactivated and Nestinpositive(Nestin;) cells aggregate at the injury site. However, the derivation of Nestin;cells is not clearly defined. Here, we found that Nestin expression was substantially increased in the lesion edge and lesion core after SCI. Using a tamoxifen inducible CreER(T2)-loxP system, we verified that ependymal cells contribute few Nestin;cells either to the lesion core or the lesion edge after SCI. In the lesion edge, GFAP+astrocytes were the main cell type that expressed Nestin;they then formed an astrocyte *** the lesion core, Nestin;cells expressed αSMA or Desmin, indicating that they might be derived from pericytes. Our results reveal that Nestin;cells in the lesion core and edge came from various cell types and rarely from ependymal cells after complete transected SCI, which may provide new insights into SCI repair.

关键词： Nestin ependymal cells spinal cord injury astrocytes pericytes

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云南省7个县村隐花青鹅膏的毒性差异研究

昆明医科大学学报 2024年第8期45卷 145-153页

作者：邱柏宁王跃兵龙武位斯杰王蕊马琳习严梅唐雪雷普平昆明医科大学法医学院云南昆明650500 云南省地方病防治所云南大理671000

目的对比云南省7个县村的隐花青鹅膏对小鼠肝脏和肾脏的毒性差异。方法将昆明小鼠随机分为7组,每组细分为6 h、3 d、7 d、14 d,4个时间点组,每个时间组包含5只小鼠。采用云南省7个县村的隐花青鹅膏进行灌胃实验,对照组(0 h)使用等量... 详细信息

目的对比云南省7个县村的隐花青鹅膏对小鼠肝脏和肾脏的毒性差异。方法将昆明小鼠随机分为7组,每组细分为6 h、3 d、7 d、14 d,4个时间点组,每个时间组包含5只小鼠。采用云南省7个县村的隐花青鹅膏进行灌胃实验,对照组(0 h)使用等量的生理盐水进行灌胃。在每次灌胃后不同时间点抽取眼球进行取血,检测血清中谷氨酰转移酶(ALT)、天门冬氨酸转氨酶(AST)、尿酸(UA)水平。同时称量小鼠肝脏和肾脏,测定其肝脏、肾脏指数,并利用HE染色观察肝、肾组织的情况。结果牟定县余丁村(MDYD)和禄劝县永翠村(LQYC)组的隐花青鹅膏对小鼠肝脏和肾脏造成急性损伤。在灌胃后6 h,小鼠血清生化指标开始上升,到第7天达到峰值,第14天开始下降,显示出损伤趋于缓解(P <0.05)。而腾冲市东华村(TCDH)组和禄劝县永翠村(LQYC)组的隐花青鹅膏仅对小鼠肝脏有损害,在第14天后血清中AST、ALT和UA水平依然呈上升趋势,表明该地区的隐花青鹅膏对肝脏影响时间较长(P <0.05)。鹤庆县磨光村(HQMG)组、南涧县德安村(NJDA)组以及牟定县高平村(MDGP)组的隐花青鹅膏对小鼠肝脏和肾脏都造成损伤,且在第14天后血清中AST、ALT和UA水平呈持续增高趋势(P <0.05),显示出小鼠的肝脏和肾脏损害并未出现改善。结论云南省不同县村的隐花青鹅膏均存在不同程度的肝脏、肾脏毒性,对肝肾有潜在危害,建议在食用隐花青鹅膏时谨慎对待。

关键词：隐花青鹅膏肝毒性肾毒性云南

Influence of Aluminum and Cadmium Stresses on Mineral Nutrition and Root Exudates in Two Barley Cultivars

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Pedosphere 2007年第4期17卷 505-512页

作者： GUO Tian-Rong ZHANG Guo-Ping ZHOU Mei-xue wu Fei-Bo CHEN Jin-Xin Agronomy Department Zhejiang University Huajiachi Campus Hangzhou 310029 （China）. University of Tasmania Tasmanian Institute of Agricultural Research Kings Meadows Tasmania 7249 （Australia） College of Life Science Shaoxing University Shaoxing 312000 （China）

A hydroponic experiment was carried out to study the effect of aluminum （Al） and cadmium （Cd） on Al and mineral nutrient contents in plants and Al-induced organic acid exudation in two barley varieties with different Al tolerance. Al- sensitive cv. Shang 70-119 had significantly higher Al content and accumulation in plants than Al-tolerant cv. gebeina, especially in roots, when subjected to low pH （4.0） and Al treatments （100 μmol L^-1 Al and 100 μmol L^-1 Al ＋1.0 μmol L^-1 Cd）. Cd addition increased Al content in plants exposed to Al stress. Both low pH and Al treatments caused marked reduction in Ca and Mg contents in all plant parts, P and K contents in the shoots and leaves, Fe, Zn and Mo contents in the leaves, Zn and B contents in the shoots, and Mn contents both in the roots and leaves. Moreover, changes in nutrient concentrations were greater in the plants exposed to both Al and Cd than in those exposed only to Al treatment. A dramatic enhancement of malate, citrate, and succinate was found in the plants exposed to 100 μmol L^-1 Al relative to the control, and the Al-tolerant cultivar had a considerable higher exudation of these organic acids than the Al-sensitive one, indicating that Al-induced enhancement of these organic acids is very likely to be associated with Al tolerance.

关键词： aluminum barley (Hordeum vulgate L.) cadmium nutrient content organic acids

核电厂大LOCA始发严重事故下氢气源项的敏感性分析

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核动力工程 2007年第5期28卷 69-74,108页

作者：郭连城曹学武上海交通大学机械与动力工程学院 200240

采用MELCOR程序,以600MW级核电厂为研究对象,在以大破口失水事故为始发事件的严重事故中,针对不同的破口尺寸及破口位置对堆芯内锆-水反应及堆腔内熔融堆芯与堆腔混凝土之间的相互作用(MCCI)中氢气源项的影响进行敏感性分析。结果表明,... 详细信息

采用MELCOR程序,以600MW级核电厂为研究对象,在以大破口失水事故为始发事件的严重事故中,针对不同的破口尺寸及破口位置对堆芯内锆-水反应及堆腔内熔融堆芯与堆腔混凝土之间的相互作用(MCCI)中氢气源项的影响进行敏感性分析。结果表明,在大破口始发的严重事故中,不同的破口尺寸对氢气源项的影响不大;而在破口尺寸相同的情况下,破口发生在主管道热段时,产氢速率的峰值最大;破口发生在主管道冷段时,累积的总产氢量最大。

关键词： MELCOR程序氢气源项敏感性分析

行业版ERP构建策略下的若干关键技术问题研究

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中国机械工程 2007年第2期18卷 187-192页

作者：吴士亮薛恒新南京财经大学南京210046 南京理工大学南京210094

针对目标行业构建行业版ERP系统是解决目前商品化ERP系统普遍存在行业针对性差、系统复杂、实施周期长等诸多问题的有效途径。给出了行业版ERP的定义,重点针对行业版ERP构建中的三类关键技术,即ERP软件系统的组件化技术、业务基础软件... 详细信息

针对目标行业构建行业版ERP系统是解决目前商品化ERP系统普遍存在行业针对性差、系统复杂、实施周期长等诸多问题的有效途径。给出了行业版ERP的定义,重点针对行业版ERP构建中的三类关键技术,即ERP软件系统的组件化技术、业务基础软件平台技术和行业版ERP软件厂商的机构与过程管理技术进行了研究。首先提出一种业务过程驱动ERP系统组件化的系统化方法,进而给出一个面向行业版ERP构建的、以系统化软件复用为主要途径的、层次化的业务基础软件平台框架,最后对行业版ERP构建策略下的软件企业的机构与过程管理问题进行了探讨。

关键词：行业版ERP 业务组件软件复用业务软件基础平台

Effective and biocompatible antibacterial surfaces via facile synthesis and surface modification of peptide polymers

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Bioactive Materials 2021年第12期6卷 4531-4541页

作者： Ziyi Lu Yueming wu Zihao Cong Yuxin Qian xue wu Ning Shao Zhongqian Qiao Haodong Zhang Yunrui She Kang Chen Hengxue Xiang Bin Sun Qian Yu Yuan Yuan Haodong Lin Meifang Zhu Runhui Liu State Key Laboratory of Bioreactor Engineering College of ChemistryChemical Engineering and Materials ScienceEast China University of Science and TechnologyShanghai200237China State Key Laboratory for Modification of Chemical Fibers and Polymer Materials College of Materials Science and EngineeringDonghua UniversityShanghai201620China Jiangsu Key Laboratory of Advanced Functional Polymers Design and Application Soochow UniversitySuzhou215123China Department of Orthopedic Surgery Shanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai200080China Key Laboratory for Ultrafine Materials of Ministry of Education Frontiers Science Center for Materiobiology and Dynamic ChemistryResearch Center for Biomedical Materials of Ministry of EducationSchool of Materials Science and EngineeringEast China University of Science and TechnologyShanghai200237China

It is an urgent need to tackle drug-resistance microbial infections that are associated with implantable biomedical *** defense peptide-mimicking polymers have been actively explored in recent years to fight against drug-resistant *** recent report on lithium hexamethyldisilazide-initiated superfast polymerization on amino acid N-carboxyanhydrides enables the quick synthesis of host defense peptide-mimicking peptide *** we reported a facile and cost-effective thermoplastic polyurethane(TPU)surface modification of peptide polymer(DLL:BLG=90:10)using plasma surface activation and substitution reaction between thiol and bromide *** peptide polymer-modified TPU surfaces exhibited board-spectrum antibacterial property as well as effective contact-killing ability in ***,the peptide polymer-modified TPU surfaces showed excellent biocompatibility,displaying no hemolysis and *** vivo study using methicillin-resistant Staphylococcus aureus(MRSA)for subcutaneous implantation infectious model showed that peptide polymer-modified TPU surfaces revealed obvious suppression of infection and great histocompatibility,compared to bare TPU *** further explored the antimicrobial mechanism of the peptide polymer-modified TPU surfaces,which revealed a surface contact-killing mechanism by disrupting the bacterial *** results demonstrated great potential of the peptide-modified TPU surfaces for practical application to combat bacterial infections that are associated with implantable materials and devices.

关键词： Peptide polymer Host defense peptide Antimicrobial surface MRSA Subcutaneous infection

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经皮穴位电刺激治疗嗓音疲劳的应用价值

中国中西医结合耳鼻咽喉科杂志 2024年第4期32卷 273-278页

作者：杨雪吴飞虎沈海龙徐可韩燕勋袁星月王琴刘业海安徽医科大学第一附属医院合肥230031 安徽中医药大学第一附属医院合肥

目的探讨经皮穴位电刺激(TEAS)在嗓音疲劳(VF)中的治疗作用。方法选取2021年4~12月就诊于安徽医科大学第一附属医院门诊并确诊为嗓音疲劳的60例患者作为研究对象,并将其随机分为两组,每组各30例,对照组行嗓音训练治疗,试验组行经皮电刺... 详细信息

目的探讨经皮穴位电刺激(TEAS)在嗓音疲劳(VF)中的治疗作用。方法选取2021年4~12月就诊于安徽医科大学第一附属医院门诊并确诊为嗓音疲劳的60例患者作为研究对象,并将其随机分为两组,每组各30例,对照组行嗓音训练治疗,试验组行经皮电刺激人迎穴及天突穴联合嗓音训练治疗,两组患者均予以治疗8周。记录两组干预前后嗓音障碍指数(VHI)、嗓音疲劳指数(VFI)及嗓音嘶哑评估表(GRBAS-G),对比干预前后的电子喉镜结果。结果两组患者治疗前VHI、VFI、GRBAS-G评分无统计学差异。治疗8周后,两组患者VHI、VFI、GRBAS-G评分较治疗前均好转,组内治疗前后对比差异有统计学意义(P<0.05)。治疗后组间比较显示试验组嗓音分值优于对照组,但VFI量表第一和第二部分及GRBAS-G评分无明显统计学差异。此外试验组总有效率显著高于对照组(P<0.05)。结论经皮无创穴位电刺激对于治疗嗓音疲劳具有良好的效果,值得进一步临床探索。

关键词：嗓音疲劳经皮穴位电刺激人迎穴天突穴

Analyzing Differences between Online Learner Groups during the COVID-19 Pandemic through K-Prototype Clustering

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Journal of Data Analysis and Information Processing 2022年第1期10卷 22-42页

作者： Guanggong ge Quanlong Guan Lusheng wu Weiqi Luo Xingyu Zhu College of Cyber Security Jinan University Guangzhou China College of Information Science and Technology Jinan University Guangzhou China Institute of Smart Education of Guangdong Jinan University Guangzhou China Science and Technology Department of Jinan University Guangzhou China

Online learning is a very important means of study, and has been adopted in many countries worldwide. However, only recently are researchers able to collect and analyze massive online learning datasets due to the COVID-19 epidemic. In this article, we analyze the difference between online learner groups by using an unsupervised machine learning technique, i.e., k-prototypes clustering. Specifically, we use questionnaires designed by domain experts to collect various online learning data, and investigate students’ online learning behavior and learning outcomes through analyzing the collected questionnaire data. Our analysis results suggest that students with better learning media generally have better online learning behavior and learning result than those with poor online learning media. In addition, both in economically developed or undeveloped regions, the number of students with better learning media is less than the number of students with poor learning media. Finally, the results presented here show that whether in an economically developed or an economically undeveloped region, the number of students who are enriched with learning media available is an important factor that affects online learning behavior and learning outcomes.

关键词： Online Learning K-Prototypes Clustering Economically Developed Region Data Analysis Different Groups Learning Behavior Learning Media

基于时空变换恒定磁化的起始磁化曲线推算方法

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物理学报 2015年第23期64卷 292-302页

作者：邓东阁武新军华中科技大学机械科学与工程学院武汉430074

起始磁化曲线作为铁磁材料磁学特性的重要表征,研究其获取方法具有重要意义.现有方法采用随时间变化磁场作为激励磁场,通过改变激励磁场大小,逐步改变试件内的磁场及磁感应强度并进行测量以得到起始磁化曲线,效率较低,准备繁琐.为此,本... 详细信息

起始磁化曲线作为铁磁材料磁学特性的重要表征,研究其获取方法具有重要意义.现有方法采用随时间变化磁场作为激励磁场,通过改变激励磁场大小,逐步改变试件内的磁场及磁感应强度并进行测量以得到起始磁化曲线,效率较低,准备繁琐.为此,本文从基本的磁学物理定理出发,提出一种基于时空变换的起始磁化曲线推算方法.该方法以细长棒状试件或环形试件作为被测试件;采用恒定磁化在被测试件内产生空间变化磁场作为激励磁场;通过测量试件表面的磁场值来推算试件内磁场值,从而获取铁磁材料起始磁化曲线.直流线圈恒定磁化环形和棒状试件仿真实验验证了该方法的理论正确性.进一步地,考虑实际应用限制因素的推算结果表明了该方法在实际应用中是可行的,可为探索新的起始磁化曲线测量方法提供理论指导.

关键词：时空变换恒定磁化起始磁化曲线