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Self-assembly of Au/MoS_(2) quantum dots core-satellite hybrid as efficient electrocatalyst for hydrogen production

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Tungsten 2020年第2期2卷 194-202页

作者： xue‑ping yu Chan Yang Pin Song Juan Peng College of Chemistry and Chemical Engineering Ningxia UniversityYinchuan 750021China School of Materials Science and Engineering Nanyang Technological UniversitySingapore 639798Singapore

Ultra-small MoS_(2) quantum dots(QDs)with an average size of 2.48 nm coated on Au nanoparticles with a core-satellite structure(Au/MoS_(2) QDs)are successfully prepared by a self-assembly strategy through the strong bonding between Au and *** from the unique structure,the Au/MoS_(2) QDs hybrid exhibits outstanding electrocatalytic performance toward hydrogen evolution reaction(HER).The Au/MoS_(2) QDs hybrid needs overpotentials of 112,146,and 206 mV to approach current densities of 10,20,and 50 mA·cm^(−2) in 0.5 mol·L^(−1) H_(2)SO_(4)(pH=0.3),*** a low Tafel slope(83 mV·dec^(−1))is *** catalyst also displays satisfactory stability and durability,indicating a negligible current density loss after 20 h of *** high hydrogen generation activities and stability are attributed to the great number of active sites,high conductivity and synergistic effects between gold nanoparticles(Au NPs)and MoS_(2) *** but not least,this strategy opens up a favorable way to combine MoS_(2) QDs with other metals.

关键词： MoS_(2)QDs Gold nanoparticles Synergistic effect Hydrogen evolution reaction

Exonuclease Ⅰ and Ⅲ improve the detection efficacy of hepatitis B virus covalently closed circular DNA

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Hepatobiliary & Pancreatic Diseases International 2019年第5期18卷 458-463页

作者： Pei-xue Jiang Ri-Cheng Mao Min-Hui Dong xue-ping yu Qi Xun Jin-yu Wang Li Jing Deng Qiang Ji-Ming Zhang Department of Infectious Diseases Huashan HospitalFudan UniversityShanghaiChina Department of Pathobiology and Key Laboratory of Medical Molecular Virology School of Basic Medical SciencesFudan UniversityShanghaiChina

Background: Hepatitis B virus covalently closed circular DNA (HBV cccDNA) is an important biomarker of hepatitis B virus infection. However, the current methods are not specific and sensitive. The present study aimed to develop a specific and sensitive assay method for the quantification of HBV cccDNA. Methods: Exonuclease Ⅰ(Exo Ⅰ)& Exonuclease Ⅲ(Exo Ⅲ) and specific primer probes are used in real-time PCR. The virus particles isolated from peripheral blood mononuclear cells were used as negative control and HBV1.3 recombinant plasmid 3.2 kb circular DNA fragment was used as positive control. The methods of cccDNA detection were evaluated in cell lines, plasmid, animal model, patient serum and liver biopsies. Results: A linear range of 10 1 –10 7 copies/assay using specific primers for HBV cccDNA was established. HBV cccDNA were only detected in cell lines, animal model and liver tissue. It cannot be detected in serum samples. Intrahepatic HBV cccDNA level had good correlation with intrahepatic total HBV DNA level ( r = 0.765, P < 0.001). Conclusions: The real-time quantitative PCR is an effective and feasible method for sensitive and specific detection of low copy number of cccDNA. The novel detection method is fast, provides high sensitivity and specificity and can be used in clinical practice.

关键词： Hepatitis B virus cccDNA Selective Sensitive

Acupuncture inhibits Notch1 and Hes1 protein expression in the basal ganglia of rats with cerebral hemorrhage

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Neural Regeneration Research 2015年第3期10卷 457-462页

作者： Wei Zou Qiu-xin Chen Xiao-wei Sun Qing-bin Chi Hong-yu Kuang xue-ping yu Xiao-hong Dai Third Department of Acupuncture First Affiliated HospitalHeilongjiang University of Chinese Medicine Heilongjiang University of Chinese Medicine Department of Endocrinology First Affiliated HospitalHarbin Medical University

Notch pathway activation maintains neural stem cells in a proliferating state and increases nerve repair capacity. To date, studies have rarely focused on changes or damage to signal transduc- tion pathways during cerebral hemorrhage. Here, we examined the effect of acupuncture in a rat model of cerebral hemorrhage. We examined four groups： in the control group, rats received no treatment. In the model group, cerebral hemorrhage models were established by infusing non-hep-arinized blood into the Brain. In the acupuncture group, modeled rats had Baihui （DU20） and Qubin （GBT） acupoints treated once a day for 30 minutes. In the DAPT group, modeled rats had 0.15 μg/mL DAPT solution （10 mL） infused into the brain. Immunohistochemistry and western blot results showed that acupuncture effectively inhibits Notch 1 and Hesl protein expression in rat basal ganglia. These inhibitory effects were identical to DAPT, a Notch signaling pathway inhibitor. Our results suggest that acupuncture has a neuroprotective effect on cerebral hemorrhage by in- hibiting Notch-Hes signaling pathway transduction in rat basal ganglia after cerebral hemorrhage.

关键词： nerve regeneration acupuncture cerebral hemorrhage immunohistochemistry westernblot assay Notch 1 lIes l rats DAPT neural stem cells NSFC grant neural regeneration

Acupuncture promotes functional recovery after cerebral hemorrhage by upregulating neurotrophic factor expression

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Neural Regeneration Research 2020年第8期15卷 1510-1517页

作者： Dan Li Qiu-Xin Chen Wei Zou Xiao-Wei Sun xue-ping yu Xiao-Hong Dai Wei Teng Third Department of Acupuncture First Affiliated HospitalHeilongjiang University of Chinese MedicineHarbinHeilongjiang ProvinceChina Department of Acupuncture Third Affiliated HospitalBeijing University of Chinese MedicineBeijingChina

Acupuncture is widely used in the treatment of cerebral hemorrhage,and it improves outcomes in experimental animal models and ***,the mechanisms underlying the effectiveness of acupuncture treatment for cerebral hemorrhage are still *** this study,a model of intracerebral hemorrhage was produced by injecting 50μL autologous blood into the caudate nucleus in Wistar *** at Baihui(DU20)and Qubin(GB7)acupoints was performed at a depth of 1.0 inch,12 hours after blood injection,once every 24 *** needle was rotated at 200 r/min for 5 minutes,For each 30-minute session,needling at 200 r/min was performed for three sessions,each lasting 5 *** the positive control group,at 6 hours,and 1,2,3 and 7 days after induction of hemorrhage,the rats were intraperitoneally injected with 1 mL aniracetam(0.75 mg/mL),three times a *** Bederson behavioral test was used to assess palsy in the contralateral *** blot assay was used to examine the expression levels of Nestin and basic fibroblast growth factor in the basal *** was performed to count the number of Nestin-and glial cell line-derived neurotrophic factor-positive cells in the basal *** effectively reduced hemorrhage and brain edema,elevated the expression levels of Nestin and basic fibroblast growth factor in the basal ganglia,and increased the number of Nestin-and glial cell line-derived neurotrophic factor-positive cells in the basal ***,these findings suggest that acupuncture promotes functional recovery after cerebral hemorrhage by increasing the expression of neurotrophic *** study was approved by the Committee for Experimental Animals of Heilongjiang Medical Laboratory Animal Center(approval No.2017061001)on June 10,2017.

关键词： acupuncture basic fibroblast growth factor brain cell protection cerebral hemorrhage electron microscope glial cell line-derived neurotrophic factor immunohistochemistry Nestin western blot assay

Baihui(DU20)-penetrating-Qubin(GB7) acupuncture inhibits apoptosis in the perihemorrhagic penumbra

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Neural Regeneration Research 2018年第9期13卷 1602-1608页

作者： Beng Zhang Xiao-Hong Dai xue-ping yu Wei Zou Wei Teng Xiao-Wei Sun Wei-Wei yu Hao Liu Hui Wang Meng-Juan Sun Meng Li Heilongjiang University of Chinese Medicine Harbin Heilongjiang Province China First Affiliated Hospital Heilongjiang University of Chinese Medicine Harbin Heilongjiang Province China Clinical Key Laboratory of Integrated Traditional Chinese and Western Medicine Heilongjiang University of Chinese Medicine HarbinHeilongjiang Province China Department of Acupuncture and Moxibustion Tongde Hospital of Zhejiang Province Hangzhou Zhejiang Province China

Baihui（DU20）-penetrating-Qubin（GB7） acupuncture can inhibit inflammatory reactions and activate signaling pathways related to proliferation after intracerebral ***,there is no research showing the relationship between this treatment and cell *** models of intracerebral hemorrhage were established by injecting 60 μL of autologous blood into the right side of the *** hours later,the needle traveled subcutaneously from the Baihui acupoint to Qubin *** needle was alternately rotated（180 ± 10 turns/min） manually along clockwise and counter-clockwise *** lasted for 7 days,and was performed three times each for 6 minutes with 6-minute intervals between *** intraperitoneally receiving Sonic hedgehog pathway activator,purmorphamine（1 mg/kg per day）,served as positive *** and sensory function were assessed using the Ludmila Belayev *** of pathological changes were measured in the perihemorrhagic penumbra using hematoxylin-eosin *** was examined by terminal deoxynucleotidyl transferase（Td T）-mediated d UTP nick end labeling *** of smoothened（Smo） and glioma-associated homolog 1（Gli1） was determined by western blot *** results showed that Baihui-penetrating-Qubin acupuncture promoted recovery of motor and sensory function,reduced the apoptotic cell percentage in the perihemorrhagic penumbra,and up-regulated Smo and Gli1 *** conclude that Baihui-penetrating-Qubin acupuncture can mitigate hemorrhage and promote functional recovery of the brain in a rat model of intracerebral hemorrhage,possibly by activating the Sonic hedgehog pathway.

关键词： nerve regeneration acupuncture treatment intracerebral hemorrhage neurological function Ludmila Belayev test TUNEL assay purmorphamine Smo Ptchl Glil neural regeneration

Effect of liver inflammation on accuracy of FibroScan device in assessing liver fibrosis stage in patients with chronic hepatitis B virus infection

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World Journal of Gastroenterology 2021年第7期27卷 641-653页

作者： Ling-Ling Huang xue-ping yu Ju-Lan Li Hui-Ming Lin Na-Ling Kang Jia-Ji Jiang yue-Yong Zhu yu-Rui Liu Da-Wu Zeng Department of Hepatology Hepatology Research InstituteThe First Affiliated HospitalFujian Medical UniversityFuzhou 350005Fujian ProvinceChina Department of Infectious Diseases The First Hospital of Quanzhou Affiliated to Fujian Medical UniversityQuanzhou 362000Fujian ProvinceChina Fujian Key Laboratory of Precision Medicine for Cancer Fujian Key Laboratory of Laboratory MedicineThe First Affiliated HospitalFujian Medical UniversityFuzhou 350005Fujian ProvinceChina

BACKGROUND Transient elastography(FibroScan)is a new and non-invasive test,which has been widely recommended by the guidelines of chronic hepatitis B virus(HBV)management for assessing hepatic fibrosis ***,some confounders may affect the diagnostic accuracy of the FibroScan device in fibrosis *** To evaluate the diagnostic value of the FibroScan device and the effect of hepatic inflammation on the accuracy of FibroScan in assessing the stage of liver fibrosis in patients with HBV *** The data of 416 patients with chronic HBV infection who accepted FibroScan,liver biopsy,clinical,and biological examination were collected from two hospitals *** operating characteristic(ROC)curves were used to analyze the diagnostic performance of FibroScan for assessing the stage of liver *** discordance in fibrosis staging by FibroScan and pathological scores was statistically *** regression and ROC analyses were used to analyze the accuracy of FibroScan in assessing the stage of fibrosis in patients with different degrees of liver inflammation.A non-invasive model was constructed to predict the risk of misdiagnosis of fibrosis stage using *** In the overall cohort,the optimal diagnostic values of liver stiffness measurement(LSM)using FibroScan for significant fibrosis(≥F2),severe fibrosis(≥F3),and cirrhosis(F4)were 7.3 kPa[area under the curve(AUC)=0.863],9.7 kPa(AUC=0.911),and 11.3 kPa(AUC=0.918),*** rate of misdiagnosis of fibrosis stage using FibroScan was 34.1%(142/416 patients).The group of patients who showed discordance between fibrosis staging using FibroScan and pathological scores had significantly higher alanine aminotransferase and aspartate aminotransferase levels,and a higher proportion of moderate to severe hepatic inflammation,compared with the group of patients who showed concordance in fibrosis staging between the two *** inflammation activity over 2(O

关键词： Liver stiffness measurement Fibrosis stage Liver inflammation Hepatitis B virus FibroScan Predictive model

Liver Stiffness Measurement can Predict Liver Inflammation in Chronic Hepatitis B Patients with Normal Alanine Transaminase

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Journal of Clinical and Translational Hepatology 2023年第4期11卷 817-826页

作者： Ling-Ling Huang xue-ping yu Qing-Fa Ruan Yan-xue Lin Huan Li Wen Jin Rui-Feng Liu Yan-Lan Liang yu-Rui Liu yue-Yong Zhu Jia-Ji Jiang Ri-Cheng Mao Da-Wu Zeng Department of Hepatology Hepatology Research InstituteThe First Affiliated hospitalFujian Medical UniversityFuzhouFujianChina Department of Infectious Diseases The First Hospital of Quanzhou Affiliated to Fujian Medical UniversityQuanzhouFujianChina Hepatology Center Xiamen Hospital of Traditional Chinese MedicineXiamenFujianChina Department of Infectious Diseases Shanghai Key Laboratory of Infectious Diseases and Biosafety Emergency ResponseNational Medical Center for Infectious DiseasesHuashan HospitalFudan UniversityShanghaiChina Hepatology Mengchao Hepatobiliary Hospital of Fujian Medical UniversityFuzhouFujianChina Hepatology Fujian Medical University Xiamen Humanity HospitalXiamenFujianChina

Background and Aims:To determine whether liver stiffness measurement(LSM)indicates liver inflammation in chronic hepatitis B(CHB)with different upper limits of normal(ULNs)for alanine aminotransferase(ALT).Methods:We grouped 439 CHB patients using different ULNs for ALT:cohort I,≤40 U/L(439 subjects);cohort II,≤35/25 U/L(males/females;330 subjects);and cohort III,≤30/19 U/L(males/females;231 subjects).Furthermore,84 and 96 CHB patients with normal ALT(≤40 U/L)formed the external and prospective validation groups,respectively We evaluated the correlation between LSM and biopsy-confirmed liver inflammation,and determined diagnostic accuracy using area under the curve(AUC).A noninvasive LSM-based model was developed using multivariate logistic ***:Fibrosis-adjusted LSM values significantly increased with increasing *** AUCs of LSM in cohorts I,II,and III were 0.799,0.796,and 0.814,respectively,for significant inflammation(A≥2)and 0.779,0.767,and 0.770,respectively,for severe inflammation(A=3).Cutoff LSM values in all cohorts for A≥2and A=3 were 6.3 and 7.5 kPa,***,external,and prospective validations showed high diagnostic accuracy of LSM for A≥2 and A=3,and no significant differences in AUCs among the four *** and globulin independently predicted A≥*** AUC of an LSM-globulin model for A≥2 exceeded those of globulin,ALT,and AST,but was similar to that of ***:LSM predicted liver inflammation and guided the indication of antiviral therapy for CHB in patients with normal ALT.

关键词： Hepatitis B virus Liver inflammation Fibrosis Liver stiffness measurement Alanine aminotransferase

Acupuncture through Baihui (DU20) to Qubin (GB7) mitigates neurological impairment after intracerebral hemorrhage

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Neural Regeneration Research 2018年第8期13卷 1425-1432页

作者： Xiao-Ying Liu Xiao-Hong Dai Wei Zou xue-ping yu Wei Teng Ying Wang Wei-Wei yu Hui-Hui Ma Qiu-Xin Chen Peng Liu Rui-Qiao Guan Shan-Shan Dong Heilongjiang University of Chinese Medicine Harbin Heilongjiang Province China The First Affiliated Hospital of Heilongjiang University of Chinese Medicine Harbin Heilongjiang Province China Clinical Key Laboratory of Integrated Traditional Chinese and Western Medicine of Heilongjiang University of Chinese Medicine HarbinHeilongjiang Province China Department of Pharmacology of Dali University Dali Yunnan Province China

Inflammation plays an important role in nerve defects caused by intracerebral hemorrhage. Repairing brain damage by inhibiting the macrophage-inducible C-type lectin/spleen tyrosine kinase （Mincle/Syk） signaling pathway is a potential new target for treating cerebral hemorrhage. In this study, we aimed to determine whether acupuncture through Baihui （DU20） to Qubin （GBT） is an effective treatment for intracerebral hemorrhage through the Mincle/Syk signaling pathway. An intracerebral hemorrhage rat model was established by autol- ogous blood infusion into the caudate nucleus. Acupuncture through Baihui to Qubin was performed for 30 minutes, once every 12 hours, for a total of three times. Piceatannol （34.62 mg/kg）, a Syk inhibitor, was intraperitoneally injected as a control. Modified neurological severity score was used to assess neurological function. Brain water content was measured. Immunohistochemistry and western blot assay were used to detect immunoreactivity and protein expression levels of Minde, Syk, and CARD9. Real-time polymerase chain reaction was used to determine interleukin-1[~ mRNA levels. Hematoxylin-eosin staining was performed to observe histopathological changes. Our re- suits showed that acupuncture through Baihui to Qubin remarkably improved neurological function and brain water content, and inhibited immunoreactivity and expression of Mincle, Syk, CARDg, and interkeukin-1β Moreover, this effect was similar to piceatannol. These find- ings suggest that acupuncture through Baihui to Qubin can improve neurological impairment after cerebral hemorrhage by inhibiting the Mincle/Syk signaling pathway.

关键词： nerve regeneration intracerebral hemorrhage acupuncture Baihui (DU20) Qubin (GBT) inflammation Mincle/Syk signaling pathway nerve protection neural regeneration

Clinical cure and liver fibrosis reversal after postoperative antiviral combination therapy in hepatitis B-associated non-cirrhotic hepatocellular carcinoma: A case report

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World Journal of Clinical Cases 2021年第3期9卷 714-721页

作者： xue-ping yu Qi Lin Zhi-Peng Huang Wei-Shan Chen Ming-Hui Zheng Yi-Juan Zheng Ju-Lan Li Zhi-Jun Su Department of Infection Diseases First Hospital of Quanzhou Affiliated to Fujian Medical UniversityQuanzhou 362000Fujian ProvinceChina Department of Gastroenterology First Hospital of Quanzhou Affiliated to Fujian Medical UniversityQuanzhou 362000Fujian ProvinceChina Department of Pathology First Hospital of Quanzhou Affiliated to Fujian Medical UniversityQuanzhou 362000Fujian ProvinceChina

BACKGROUND The incidence of hepatocellular carcinoma(HCC)is high in China,and approximately 15%-20%of HCC cases occur in the absence of *** with patients with cirrhotic HCC,those with non-cirrhotic HCC have longer postoperative tumor-free ***,the overall survival time is not significantly increased,and the risk of postoperative recurrence *** to improve the postoperative survival rate in these patients are currently *** SUMMARY A 47-year-old man with a family history of HCC was found to have hepatitis B virus(HBV)infection 25 years *** 2000,he was administered lamivudine for 2 years,and entecavir(ETV 0.5 mg)was administered in *** October 2016,magnetic resonance imaging revealed a tumor in the liver(5.3 cm×5 cm×5 cm);no intraoperative hepatic and portal vein and bile duct tumor thrombi were found;and postoperative pathological examination confirmed a grade II HCC with no nodular cirrhosis(G1S3).ETV was continued,and no significant changes were observed on *** receiving pegylated interferon alfa-2b(PEG IFNα-2b)(180μg)+ETV in February 2019,the HBsAg titer decreased significantly within 12 *** receiving hepatitis B vaccine(60μg)in 12 wk,HBsAg serological conversion was realized at 48 *** the treatment,no obvious adverse reactions were observed,except for early alanine aminotransferase *** reexamination results of liver pathology were G2S1,and reversal of liver fibrosis was *** The therapeutic regimen of ETV+PEG IFNα-2b+hepatitis B vaccine for patients with HBV-associated non-cirrhotic HCC following hepatectomy can achieve an HBV clinical cure and prolong the recurrence-free survival.

关键词： Hepatocellular carcinoma Hepatitis B virus Entecavir Pegylated interferon alfa-2b Clinical cure Case report