Acupuncture through Baihui (DU20) to Qubin (GB7) mitigates neurological impairment after intracerebral hemorrhage

作     者：Xiao-Ying Liu Xiao-Hong Dai Wei Zou Xue-Ping Yu Wei Teng Ying Wang Wei-Wei Yu Hui-Hui Ma Qiu-Xin Chen Peng Liu Rui-Qiao Guan Shan-Shan Dong 

作者机构：Heilongjiang University of Chinese Medicine Harbin Heilongjiang Province China The First Affiliated Hospital of Heilongjiang University of Chinese Medicine Harbin Heilongjiang Province China Clinical Key Laboratory of Integrated Traditional Chinese and Western Medicine of Heilongjiang University of Chinese Medicine HarbinHeilongjiang Province China Department of Pharmacology of Dali University Dali Yunnan Province China 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2018年第13卷第8期

页      面：1425-1432页

核心收录：

学科分类：1005[医学-中医学] 100512[医学-针灸推拿学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China,No.81473764,81273824 the Key Project of Natural Science Foundation of Heilongjiang Province of China,No.ZD201204 the Doctoral Fund Program of Ministry of Education of China,No.20102327110003 

主　　题：nerve regeneration intracerebral hemorrhage acupuncture Baihui (DU20) Qubin (GBT) inflammation Mincle/Syk signaling pathway nerve protection neural regeneration 

摘      要：Inflammation plays an important role in nerve defects caused by intracerebral hemorrhage. Repairing brain damage by inhibiting the macrophage-inducible C-type lectin/spleen tyrosine kinase （Mincle/Syk） signaling pathway is a potential new target for treating cerebral hemorrhage. In this study, we aimed to determine whether acupuncture through Baihui （DU20） to Qubin （GBT） is an effective treatment for intracerebral hemorrhage through the Mincle/Syk signaling pathway. An intracerebral hemorrhage rat model was established by autol- ogous blood infusion into the caudate nucleus. Acupuncture through Baihui to Qubin was performed for 30 minutes, once every 12 hours, for a total of three times. Piceatannol （34.62 mg/kg）, a Syk inhibitor, was intraperitoneally injected as a control. Modified neurological severity score was used to assess neurological function. Brain water content was measured. Immunohistochemistry and western blot assay were used to detect immunoreactivity and protein expression levels of Minde, Syk, and CARD9. Real-time polymerase chain reaction was used to determine interleukin-1[~ mRNA levels. Hematoxylin-eosin staining was performed to observe histopathological changes. Our re- suits showed that acupuncture through Baihui to Qubin remarkably improved neurological function and brain water content, and inhibited immunoreactivity and expression of Mincle, Syk, CARDg, and interkeukin-1β Moreover, this effect was similar to piceatannol. These find- ings suggest that acupuncture through Baihui to Qubin can improve neurological impairment after cerebral hemorrhage by inhibiting the Mincle/Syk signaling pathway.