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Thymoquinone affects hypoxia-inducible factor-1αexpression in pancreatic cancer cells via HSP90 and PI3K/AKT/mTOR pathways

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World Journal of Gastroenterology 2024年第21期30卷 2793-2816页

作者： Zhan-xue zhao Shuai Li Lin-Xun Liu Department of General Surgery Qinghai Provincial People's HospitalXining 810007Qinghai ProvinceChina Department of Clinical Pharmacy The Affiliated Hospital of Qinghai UniversityXining 810001Qinghai ProvinceChina

BACKGROUND Pancreatic cancer(PC)is associated with some of the worst prognoses of all major ***(TQ)has a long history in traditional medical practice and is known for its anti-cancer,anti-inflammatory,anti-fibrosis and antioxidant pharmacological *** studies on hypoxia-inducible factor-1α(HIF-1α)and PC have shown that HIF-1αaffects the occurrence and development of PC in many *** addition,TQ could inhibit the development of renal cancer by decreasing the expression of HIF-1α.Therefore,we speculate whether TQ affects HIF-1αexpression in PC cells and explore the *** To elucidate the effect of TQ in PC cells and the regulatory mechanism of HIF-1α*** Cell counting kit-8 assay,Transwell assay and flow cytometry were performed to detect the effects of TQ on the proliferative activity,migration and invasion ability and apoptosis of PANC-1 cells and normal pancreatic duct epithelial(hTERTHPNE)*** real-time polymerase chain reaction and western blot assay were performed to detect the expression of HIF-1αmRNA and protein in PC *** effects of TQ on the HIF-1αprotein initial expression pathway and ubiquitination degradation in PANC-1 cells were examined by western blot assay and *** TQ significantly inhibited proliferative activity,migration,and invasion ability and promoted apoptosis of PANC-1 cells;however,no significant effects on hTERT-HPNE cells were *** significantly reduced the mRNA and protein expression levels of HIF-1αin PANC-1,AsPC-1,and BxPC-3 *** significantly inhibited the expression of the HIF-1αinitial expression pathway(PI3K/AKT/mTOR)related proteins,and promoted the ubiquitination degradation of the HIF-1αprotein in PANC-1 *** had no effect on the hydroxylation and von Hippel Lindau protein mediated ubiquitination degradation of the HIF-1αprotein but affected the stability of the HIF-1αprotein by inhibiting the interaction between HI

关键词： Thymoquinone Pancreatic cancer Hypoxia-inducible factor-1α PI3K/AKT/mTOR HSP90

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A Layered Energy-Efficient Multi-Node Scheduling Mechanism for Large-Scale WSN

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Computers, Materials & Continua 2024年第4期79卷 1335-1351页

作者： xue zhao Shaojun Tao Hongying Tang Jiang Wang Baoqing Li Science and Technology on Microsystem Laboratory Shanghai Institute of Microsystem and Information TechnologyChinese Academy of SciencesShanghai200050China

In recent years, target tracking has been considered one of the most important applications of wireless sensornetwork (WSN). Optimizing target tracking performance and prolonging network lifetime are two equally criticalobjectives in this scenario. The existing mechanisms still have weaknesses in balancing the two demands. Theproposed heuristic multi-node collaborative scheduling mechanism (HMNCS) comprises cluster head (CH)election, pre-selection, and task set selectionmechanisms, where the latter two kinds of selections forma two-layerselection mechanism. The CH election innovatively introduces the movement trend of the target and establishesa scoring mechanism to determine the optimal CH, which can delay the CH rotation and thus reduce energyconsumption. The pre-selection mechanism adaptively filters out suitable nodes as the candidate task set to applyfor tracking tasks, which can reduce the application consumption and the overhead of the following task setselection. Finally, the task node selection is mathematically transformed into an optimization problem and thegenetic algorithm is adopted to form a final task set in the task set selection mechanism. Simulation results showthat HMNCS outperforms other compared mechanisms in the tracking accuracy and the network lifetime.

关键词： Node scheduling pre-selection target tracking WSN

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Ultrafine nano-copper derived from dopamine polymerization&synchronous adsorption achieve electrochemical purification of nitrate to ammonia in complex water environments

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Chinese Chemical Letters 2024年第8期35卷 383-390页

作者： xue zhao Mengshan Chen Dan Wang Haoran Zhang Guangzhi Hu Yingtang Zhou Faculty of Chemistry and Chemical Engineering Yunnan Normal UniversityKunming 650092China National Engineering Research Center for Marine Aquaculture Marine Science and Technology CollegeZhejiang Ocean UniversityZhoushan 316004China College of Chemistry Nankai University Nankai UniversityTianjing 300071China Institute for Ecological Research and Pollution Control of Plateau Lakes School of Ecology and Environmental ScienceYunnan UniversityKunming 650504China

Electrochemical-nitrate-reduction-reaction(eNitRR)synthesis of ammonia is an effective way to treat ni-trate wastewater and alleviate the pressure of the Haber-Bosch ammonia production *** to develop effective catalysts to electrochemically reduce nitrate to ammonia and purify sewage under com-plex environmental conditions is the focus of current ***,the dopamine polymerization pro-cess and the[(C_(12)H_(8)N_(2))_(2)Cu]^(2+)complex embedding process were run simultaneously in time and space,and ultrafine Cu nanoparticles(Cu/CN)were effectively loaded on nitrogen-doped carbon after heat *** Cu/CN as the catalyst,the ammonia yield rate and Faradaic efficiency of the electrochemical conversion of NO_(3)^(-)to NH_(3)are highly 8984.0μg h^(−1)mg cat.^(−1)and 95.6%,*** in the face of complex water environments,such as neutral media,acidic media,coexisting ions,and actual nitrate wastewater,nitrate wastewater can be effectively purified to form high value-added *** strat-egy of simultaneous embedding increases the exposure rate of Cu sites,and the support of CN is also beneficial to reduce the energy barrier of ^(∗)NO_(3)*** study rationally designed catalysts that are beneficial to eNitRR,and considered the situation faced by practical applications during the research stage,reducing the performance gap between laboratory exploration and industrial applications.

关键词： Electrochemical nitrate reduction reaction Synthetic ammonia Sewage treatment Nano copper Dopamine

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Fully sprayed MXene-based high-performance flexible piezoresistive sensor for image recognition

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Nano Materials Science 2024年第1期6卷 77-85页

作者： Zhi-Dong Zhang xue-Feng zhao Qing-Chao Zhang Jie Liang Hui-Nan Zhang Tian-Sheng Zhang Chen-Yang xue Key Laboratory of Instrumentation Science&Dynamic Measurement of Ministry of Education North University of ChinaTaiyuan030051People's Republic of China State Key Laboratory of ASIC and System Shanghai Institute of Intelligent Electronics&SystemsSchool of MicroelectronicsFudan UniversityShanghai200433People's Republic of China Shanxi Hosipital of Acupuncture and Moxibustion Taiyuan030006People's Republic of China

High-performance flexible pressure sensors provide comprehensive tactile perception and are applied in human activity monitoring,soft robotics,medical treatment,and human-computer ***,these flexible pressure sensors require extensive nano-architectural design and complicated manufacturing and are ***,a highly sensitive,flexible piezoresistive tactile sensor is designed and fabricated,consisting of three main parts:the randomly distributed microstructure on T-ZnOw/PDMS film as a top substrate,multilayer Ti_(3)C_(2)-MXene film as an intermediate conductive filler,and the few-layer Ti_(3)C_(2)-MXene nanosheetbased interdigital electrodes as the bottom *** MXene-based piezoresistive sensor with randomly distributed microstructure exhibits a high sensitivity over a broad pressure range(less than 10 kPa for 175 kPa^(-1))and possesses an out-standing permanence of up to 5000 ***,a 16-pixel sensor array is designed,and its potential applications in visualizing pressure distribution and an example of tactile feedback are *** fully sprayed MXene-based pressure sensor,with high sensitivity and excellent durability,can be widely used in,electronic skin,intelligent robots,and many other emerging technologies.

关键词： Piezoresistive sensor Ti_(3)C_(2)-MXene T-ZnOw/PDMS film Randomly distributed microstructure

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A chromatographic method for pursuing potential GPCR ligands with the capacity to characterize their intrinsic activities of regulating downstream signaling pathway

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Chinese Chemical Letters 2024年第10期35卷 451-455页

作者： Ting Li Xinxin Zheng Lejing Qu Yuanyuan Ou Sai Qiao xue zhao Yajun Zhang Xinfeng zhao Qian Li Key Laboratory of Resource Biology and Biotechnology in Western China Ministry of Education College of Life Sciences Northwest University

GPCRs are dominant targets for approved drugs and the discovery of lead compound targeting them is still challengeable. Affinity-based screening technique is a promising platform to uncover GPCR ***, the intrinsic activities of them are seldom simultaneously determined during the *** beta2-adrenoceptor（β2AR） as a probe, this work created a strategy for screening GPCR ligands with simultaneous characterization of their downstream G protein binding responses associated with GTP. The strategy included（i） the design and expression of a protein miniature formed by β2AR and G protein α-subunit（Gαs） using circularly permuted HaloTag（cpHalo） as a flexible linker;（ii） immobilization of the miniature onto silica gel by a click dehalogenation reaction;（iii） systematic characterization of the immobilized miniature by fluorescent and chromatographic studies, and（iv） simulating of ligand-induced β2AR-Gαssignaling cascade by chromatographic assays using GTP as an indicator. The immobilized miniature exhibited specificity to β2AR and Gαsantibodies and ligands. The specificity is stable at least within fifteen days with the variation less than 1%. The intrinsic activities of β2AR ligands were distinguished by the changes of GTP chromatographic behaviors on Gαs-cpHalo-β2AR *** strengthened the binding affinity and kinetics of GTP with Gαs, while antagonist did not give any effect on them. With the intrinsic activity evaluation, we believe, it will improve the attributes of chromatographic methods for drug discovery efforts with minimizing false-positive results.

关键词： Agonist/antagonist Circularly permuted HaloTag GPCR-G protein miniature GTP binding High-performance receptor chromatography Intrinsic efficacy

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Plutonium utilization in a small modular molten-salt reactor based on a batch fuel reprocessing scheme

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Nuclear Science and Techniques 2024年第4期35卷 15-28页

作者： xue-Chao zhao Rui Yan Gui-Feng Zhu Ya-Fen Liu Jian Guo Xiang-Zhou Cai Yang Zou Shanghai Institute of Applied Physics Chinese Academy of SciencesShanghai 201800China CAS Innovative Academies in TMSR Energy System Chinese Academy of SciencesShanghai 201800China University of Chinese Academy of Sciences Beijing 100049China

A molten salt reactor(MSR)has outstanding features considering the application of thorium fuel,inherent safety,sustainability,and resistance to ***,fissile material^(233)U is significantly rare at the current stage,thus it is difficult for MSR to achieve a pure thorium-uranium fuel ***,using plutonium or enriched uranium as the initial fuel for MSR is more *** this study,we aim to verify the feasibility of a small modular MSR that utilizes plutonium as the starting fuel(SM-MSR-Pu),and highlight its advantages and ***,the structural design and fuel management scheme of the SM-MSR-Pu were ***,the neutronic characteristics,such as the graphite-irradiation lifetime,burn-up performance,and coefficient of temperature reactivity were calculated to analyze the physical characteristics of the *** results indicate that plutonium is a feasible and advantageous starting fuel for a SM-MSR;however,there are certain shortcomings that need to be *** a 250 MWth SM-MSR-Pu,approximately 288.64 kg^(233)U of plutonium with a purity of greater than 90% is produced while 978.00 kg is burned every ten *** temperature reactivity coefficient decreases from -4.0 to -6.5 pcm K^(-1) over the 50-year operating time,which ensures a long-term safe ***,the amount of plutonium and accumulation of minor actinides(MAs)would increase as the burn-up time increases,and the annual production and purity of^(233)U will *** achieve an optimal burn-up performance,setting the entire operation time to 30 years is ***,more than 3600 kg of plutonium eventually accumulate in the *** research is required to effectively utilize this accumulated plutonium.

关键词： Molten salt fuel Plutonium utilization ^(233)U TRUs mole fraction Temperature feedback coefficient

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Application of multidisciplinary in situ simulation training in the treatment of acute ischemic stroke: a quality improvement project

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World Journal of Emergency Medicine 2024年第1期15卷 41-46页

作者： Ganying Huang Huijie Yang Huan Yao Xinxin Fan Wenqin Xia Yuansheng Xu Xiaoling Shen xue zhao Fourth School of Clinical Medicine Zhejiang Chinese Medical UniversityHangzhou 310053China Emergency Medicine Departent Hangzhou First People's HospitalHangzhou 310006China

BACKGROUND:Ischemic stroke refers to a disorder in the blood supply to a local area of brain tissue for various reasons and is characterized by high morbidity,mortality,and *** reperfusion of brain tissue at risk of injury is crucial for the treatment of acute ischemic *** purpose of this study was to evaluate comfort levels in managing acute stroke patients with hypoxemia who required endotracheal intubation after multidisciplinary in situ simulation training and to shorten the door-to-image ***:This quality improvement project utilized a comprehensive multidisciplinary in situ simulation exercise.A total of 53 participants completed the two-day in situ simulation *** main outcome was the self-reported comfort levels of participants in managing acute stroke patients with hypoxemia requiring endotracheal intubation before and after simulation training.A 5-point Likert scale was used to measure participant comfort.A paired-sample t-test was used to compare the mean self-reported comfort scores of participants,as well as the endotracheal intubation time and door-to-image time on the fi rst and second days of in situ simulation *** door-to-image time before and after the training was also ***:The findings indicated that in situ simulation training could enhance participant comfort when managing acute stroke patients with hypoxemia who required endotracheal intubation and shorten door-to-image *** the emergency management of hypoxemia or tracheal intubation,the mean post-training self-reported comfort score was signifi cantly higher than the mean pre-training comfort score(hypoxemia:4.53±0.64 vs.3.62±0.69,t=-11.046,P<0.001;tracheal intubation:3.98±0.72 vs.3.43±0.72,t=-6.940,P<0.001).We also observed a decrease in the tracheal intubation and door-to-image time and a decreasing trend in the door-to-image time,which continued after the ***:Our study demonstrates that the implementation of in s

关键词： Ischemic stroke Endotracheal intubation Door-to-image time In situ simulation Multidisciplinary approach

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METTL5 promotes cell proliferation,invasion,and migration by up-regulating Toll-like receptor 8 expression in colorectal cancer

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World Journal of Gastrointestinal Oncology 2024年第5期16卷 2006-2017页

作者： Ling-Shang Kong Ran Tao Yi-Fan Li Wen-Bin Wang xue zhao Department of General Surgery The Second Affiliated Hospital of Anhui Medical UniversityHefei 230001Anhui ProvinceChina Department of Vascular Surgery The Second Affiliated Hospital of Anhui Medical UniversityHefei 230002Anhui ProvinceChina Department of General Surgery The First Affiliated Hospital of Anhui Medical UniversityHefei 230001Anhui ProvinceChina Anhui Public Health Clinical Center Hefei 230012Anhui ProvinceChina Department of Scientific Research Anhui Public Health Clinical CenterHefei 230012Anhui ProvinceChina Department of Scientific Research The First Affiliated Hospital of Anhui Medical UniversityHefei 230001Anhui ProvinceChina

BACKGROUND N6-methyladenosine(m6A)modification represents the predominant alteration found in eukaryotic messenger RNA and plays a crucial role in the progression of various ***,despite its significance,the comprehensive investigation of METTL5,a key m6A methyltransferase,in colorectal cancer(CRC)remains *** To investigate the role of METTL5 in *** We assessed METTL5 expression levels in clinical samples obtained from CRC patients as well as in CRC cell *** elucidate the downstream targets of METTL5,we performed RNA-sequencing analysis coupled with correlation analysis,leading us to identify Toll-like receptor 8(TLR8)as a potential downstream *** vitro functional assessments of METTL5 and TLR8 were conducted using CCK-8 assays,scratch assays,as well as assays measuring cell migration and *** Our findings reveal a pronounced upregulation of METTL5 expression in both CRC cells and tissues,which correlated significantly with an unfavorable *** vitro experiments unequivocally demonstrated the oncogenic role of METTL5,as evidenced by its promotion of CRC cell proliferation,invasion,and ***,we identified TLR8 as a downstream target of METTL5,and subsequent down-regulation of TLR8 led to a significant inhibition of CRC cell proliferation,invasion,and tumor *** The heightened expression of METTL5 in CRC is strongly associated with clinicopathological features and a poor prognosis,thereby underscoring its potential utility as a critical marker for facilitating early diagnosis and prognostication in CRC.

关键词： METTL5 Toll-like receptor 8 Colorectal cancer

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Mechanical performance of 22SiMn2TiB steel welded with lowtransformation- temperature filler wire and stainless steel filler wire

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Journal of Iron and Steel Research International 2024年第4期31卷 967-981页

作者： Zi-dong Lin Kai-jie Song Zhen Sun Zi-qian Zhu xue-feng zhao Constantinos Goulas Wei Ya Xing-hua Yu School of Materials Science and Engineering Beijing Institute of TechnologyBeijing 100081China Beijing Institute of Technology Chongqing Innovation Center Chongqing 401120China Department of Design Production and Management Faculty of Engineering Technology.University of TwenteEnschede 7500 AETheNetherlands Rotterdam Additive Manufacture Fieldlab(RAMLAB) Rotterdam 3089 JWTheNetherlands

TX-80 low-transformation-temperature(LTT)welding wire was used to replace the traditional ER 307Si welding wire to realize the connection of 22SiMn2TiB armor steel in manual overlay *** previously existing issues,such as welding cracks,large welding deformation,and severe welding residual stress,were solved to ensure good strength and ductility *** particular,with the same welding conditions,TX-80 LTT wire eliminates welding *** reduces the welding deformation no matter the base pretreatment of pre-setting angle or no pre-setting *** comparison,it was found that the microstructure at the TX-80 weld is mainly composed of martensite and a small amount of retained *** contrast,the microstructure of the ER 307Si weld consists of a large amount of austenite and a small amount of skeleton-like *** variation trend of residual stress and microhardness from the weld to the base were investigated and compared with the mechanical properties of base *** TX-80 and the ER 307Si tensile samples elongation is 6.76%and 6.01%,while the ultimate tensile strengths are 877 and 667 MPa,*** average impact toughness at room temperature of the ER 307Si weld is 143.9 J/cm^(2),much higher than that of the TX-80 weld,which is only 36.7 J/cm^(2).The relationship between impact and tensile properties with microstructure species and distribution was *** addition,the fracture surface of the tensile and the impact samples was observed and *** dimples,fewer pores,larger radiation zone,and shear lips of TX-80 samples indicate better tensile ductility and worse impact toughness than those of ER 307Si weld.

关键词： Low-transformation-temperature material 22SiMn2TiB steel Welding deformation Residual stress Martensitic transformation Mechanical property

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Adenosine triphosphate mediates the pain tolerance effect of manual acupuncture at Zusanli(ST36) in mice

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Journal of Traditional Chinese Medicine 2024年第4期44卷 660-669页

作者： LI Zhongzheng zhao Yadan Ma Weigang Zhang Yonglong XU Zhifang XI Qiang LI Yanqi QIN Siru ZHANG Zichen WANG Songtao zhao xue LIU Yangyang GUO Yi GUO Yongming Research Center of Experimental Acupuncture Science Tianjin University of Traditional Chinese MedicineTianjin 301617China National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion Tianjin 301617China Department of Acupuncture and Moxibustion Qingdao Hiser Hospital Affiliated of Qingdao University(Qingdao Traditional Chinese Medicine Hospital)Qingdao 266033China School of Traditional Chinese Medicine Tianjin University of Traditional Chinese MedicineTianjin 301617China School of Acupuncture and Moxibustion and Tuina Tianjin University of Traditional Chinese MedicineTianjin 301617China

OBJECTIVE:To investigate the mechanisms behind the effects of acupuncture in Traditional Chinese Medicine,we delved into the adenosine triphosphate/peripheral purinergic P2X receptor 3(ATP/P2X3)receptor signaling system as an indicator of the body's energy state,commonly referred to as"Qi".METHODS:The tail-flick test was utilized to explore the impact of acupuncture on pain tolerance threshold(PTT)in mice,while also assessing adenosine(ADO)levels and adenylate energy charge(EC)at Zusanli(ST36).The study further investigated the dose-dependent effects of acupuncture on PTT and ADO levels at Zusanli(ST36).To shed light on the underlying mechanisms of acupuncture's effects,the study examined the impact of ATP,a P2X3 receptor antagonist,and adenosine disodium on PTT following acupuncture ***:Acupuncture at Zusanli(ST36)led to significant improvements in PTT in mice,with the most effective interventions being twirling for 2 min and needle retention for 28 *** interventions also resulted in significant increases in ATP *** effects of acupuncture were further augmented by administration of different doses of ATP at Zusanli(ST36),and pretreatment with a P2X3 receptor antagonist decreased *** EC peaked at 30 min after intraperitoneal injection of ATP,and pretreatment with various doses of *** 30 min prior to acupuncture increased PTT in a dose-dependent ***,pretreatment with an *** intramuscular injection of adenosine disodium enhanced the effects of ***:This research provides compelling evidence that ATP is involved in the regulation of PTT through acupuncture,revealing new avenues for achieving enhanced clinical outcomes.

关键词： acupuncture point ST36(Zusanli) adenosine triphosphate receptors,purinergic P2X3 energy state

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