METTL5 promotes cell proliferation,invasion,and migration by up-regulating Toll-like receptor 8 expression in colorectal cancer

作     者：Ling-Shang Kong Ran Tao Yi-Fan Li Wen-Bin Wang Xue Zhao 

作者机构：Department of General SurgeryThe Second Affiliated Hospital of Anhui Medical UniversityHefei 230001Anhui ProvinceChina Department of Vascular SurgeryThe Second Affiliated Hospital of Anhui Medical UniversityHefei 230002Anhui ProvinceChina Department of General SurgeryThe First Affiliated Hospital of Anhui Medical UniversityHefei 230001Anhui ProvinceChina Anhui Public Health Clinical CenterHefei 230012Anhui ProvinceChina Department of Scientific ResearchAnhui Public Health Clinical CenterHefei 230012Anhui ProvinceChina Department of Scientific ResearchThe First Affiliated Hospital of Anhui Medical UniversityHefei 230001Anhui ProvinceChina 

出 版 物：《World Journal of Gastrointestinal Oncology》 (世界胃肠肿瘤学杂志（英文版）（电子版）)

年 卷 期：2024年第16卷第5期

页      面：2006-2017页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：Supported by Natural Science Foundation in Anhui Province of China,No.2008085MH279 Key Project of Anhui Translational Medicine Research Institute,No.2022zhyx-B08 

主　　题：METTL5 Toll-like receptor 8 Colorectal cancer 

摘      要：BACKGROUND N6-methyladenosine(m6A)modification represents the predominant alteration found in eukaryotic messenger RNA and plays a crucial role in the progression of various ***,despite its significance,the comprehensive investigation of METTL5,a key m6A methyltransferase,in colorectal cancer(CRC)remains *** To investigate the role of METTL5 in *** We assessed METTL5 expression levels in clinical samples obtained from CRC patients as well as in CRC cell *** elucidate the downstream targets of METTL5,we performed RNA-sequencing analysis coupled with correlation analysis,leading us to identify Toll-like receptor 8(TLR8)as a potential downstream *** vitro functional assessments of METTL5 and TLR8 were conducted using CCK-8 assays,scratch assays,as well as assays measuring cell migration and *** Our findings reveal a pronounced upregulation of METTL5 expression in both CRC cells and tissues,which correlated significantly with an unfavorable *** vitro experiments unequivocally demonstrated the oncogenic role of METTL5,as evidenced by its promotion of CRC cell proliferation,invasion,and ***,we identified TLR8 as a downstream target of METTL5,and subsequent down-regulation of TLR8 led to a significant inhibition of CRC cell proliferation,invasion,and tumor *** The heightened expression of METTL5 in CRC is strongly associated with clinicopathological features and a poor prognosis,thereby underscoring its potential utility as a critical marker for facilitating early diagnosis and prognostication in CRC.