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Morphology and leaf nutrition of introduced Robinia pseudoacacia clones

Forestry Studies in China 2013年第1期15卷 24-31页

作者： Guo-Jun ZHANG jiang-Jie SHEN Li-mei ZHAO Yu-Han SUN Yun LI Zhao-He xu Peng SUN National Engineering Laboratory for Tree Breeding Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education College of Biological Sciences and Technology Beijing Forestry University College of Horticultural Science and Technology Hebei Normal University of Science & Technology College of Forestry Key Laboratory for Silviculture and Conservation of Ministry of Education Beijing Forestry University

In order to study and popularize clones from the introduced Robinia pseudoacacia, morphological charac- teristics and leaf nutrition of samples of a three-year-old stand were observed and analyzed during its growing season. Combined with data on rooting ability, height and basal diameter, comparisons were made on morphological characteris- tics, growth traits and leaf nutrition of 11 introduced R. pseudoacacia clones （two from Hungary and nine from South Ko- rea） and two domestic clones, The results show that there are significant differences in growth and morphological char- acteristics among the 13 clones, but no significant differences in the contents of crude protein and crude fibers in the leaves （p 〉 0.05）. Height and basal diameter growth of clones B and G were recorded as the fastest, while clone H1 was the slowest. Clone H2 had the largest leaflets, three times as large as other clones, while K4 had the heaviest dry weight per 100 leaflets because of its thick leaves. The 13 clones can be divided into four classes based on the number of leaf- lets per compound leaf, i.e., 1-3, 13-17, 15-23 and 21-25. There were significant differences in thorn size： H2, with the largest leaflets had the smallest thorns. While of course all clones produced roots, there were significant differences; clones 2N, K5 and B had many adventitious roots, while clones K3, K4 and H1 had few. Correlation between content of crude protein and （length x width of leaf） was positive （p 〈 0.05）, while correlations of the content of crude protein, with the number of leaflets per compound leaf, petiole length of compound leaf and thorn length were negative （p 〈 0.01）. Plant height and basal diameter were positively correlated with each other （p 〈 0.01） and negatively correlated with base width of thorns （p 〈 0.05）.

关键词： Robinia pseudoacacia morphological characteristics leaf nutrition growth characteristics rooting ability

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HLA-mismatched allogeneic adoptive immune therapy in severely immunosuppressed AIDS patients

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Signal Transduction and Targeted Therapy 2021年第6期6卷 1780-1787页

作者： Ruonan xu Ji-Yuan Zhang Bo Tu Zhe xu Hui-Huang Huang Lei Huang Yan-mei Jiao Tao Yang Chao Zhang En-Qiang Qin Tian-Jun jiang Yun-Bo Xie Yuan-Yuan Li Lei Jin Chun-Bao Zhou Ming Shi mei Guo Hui-Sheng Ai Linqi Zhang Fu-Sheng Wang Treatment and Research Center for Infectious Diseases The Fifth Medical CenterPLA General HospitalBeijingChina Department of Hematology and Transplantation The Fifth Medical CenterPLA General HospitalBeijingChina Comprehensive AIDS Research Center School of MedicineTsinghua UniversityBeijingChina

Severely immunosuppressed AIDS patients with recurrent opportunistic infections(Ols)represent an unmet medical need even in the era of antiretroviral therapy(ART).Here we report the development of a human leukocyte antigen(HLA)-mismatched allogeneic adaptive immune therapy(AAIT)for severely immunosuppressed AIDS *** severely immunosuppressed AIDS patients with severe Ols were enrolled in this single-arm *** donors received subcutaneous recombinant granulocyte-colony-stimulating factor twice daily for 4-5 days to stimulate *** blood mononuclear cells were collected from these donors via leukapheresis and transfused into the coupled ***,immunological,and virological parameters were monitored during a 12-month follow-up *** found AAIT combined with ART was safe and well-tolerated at the examined doses and transfusion regimen in all 12 *** in clinical symptoms were evident throughout the study *** patients exhibited a steady increase of peripheral CD4^(+)T cells from a median 10.5 to 207.5 cells/μ*** increase in peripheral CD8^(+)T-cell count from a median 416.5 to 1206.5 cells/μl was found in the first 90 days since initiation of *** addition,their inflammatory cytokine levels and HIV RNA viral load decreased.A short-term microchimerism with donor cells was *** were no adverse events associated with graft-versus-host disease throughout the study ***,AAIT treatment was safe,and might help severely immunosuppressed AIDS patients to achieve a better immune restoration.A further clinical trial with control is necessary to confirm the efficacy of AAIT medication.

关键词： patients doses transfusion

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Network pharmacology-based prediction and verification of the mechanism for Bushen Chengyun granule on low endometrial receptivity

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Journal of Traditional Chinese Medical Sciences 2020年第1期7卷 2-11页

作者： Ling Huang Tiegang Liu mei jiang Chen Bai Jingnan xu Shaoyang Liu Ning Kang Ghulam Murtaza He Yu Xiaohong Gu School of Traditional Chinese Medicine Beijing University of Chinese MedicineBeijing102488China School of Life Sciences Beijing University of Chinese MedicineBeijing102488China Department of Acupuncture and Minimally Invasive Oncology Beijing University of Chinese Medicine Third Affiliated HospitalBeijing100029China China Association of Chinese Medicine Beijing100029China Department of Pharmacy COMSATS Institute of Information TechnologyAbbottabad22060Pakistan

Objective:Bushen Chengyun granule(BCG)is an empirical treatment for female infertility(FI)caused by low endometrial receptivity(LER)involving a poorly understood *** this study,network pharmacology was used to explore the potential therapeutic mechanism of BCG on FI caused by ***:The corresponding herb targets were obtained by conducting a search in the Traditional Chinese Medicine Systems Pharmacology Database and PubMed-reported *** targets were obtained from the following databases:Comparative Toxicogenomics Database,Human Phenotype Ontology,and Therapeutic Target *** for LER using BCG have used target matching(BCG e LER target).Then,the predicted targets were uploaded to the Search Tool for the Retrieval of Interacting Genes/Proteins database for gene ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway ***,triptorelin acetate for injection t menotrophin t chorionic gonadotropin for injection were used to establish a mouse model of blastocyst implantation disorder and to evaluate the in vivo effect of BCG on blastocyst ***:Overall,156 bioactive chemical components and 1092 targets of BCG were *** results indicated that 482 biological processes(FDR<0.01)and 15 pathways(FDR<0.01)related to BCG participated in the complex treatment effects and were associated with the endocrine system,inflammatory responses,metabolism,apoptosis,ovulatory performance,and ***,16 hub nodes of BCG including estrogen receptor(ESR1),estrogen receptor beta(ESR2),progesterone receptor,et al,were recognized as potential treatment targets and might help clarify the underlying therapeutic mechanisms of BCG for female *** significantly increased the protein expressions of estrogen receptors and progesterone ***:These findings reveal the potential therapeutic mechanism of BCG for female infertility involves low endometrial receptivity,wh

关键词： Bushen Chengyun granule Low endometrial receptivity Female infertility Network pharmacology

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Transcriptomic responses to aluminum(Al)stress in maize

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Journal of Integrative Agriculture 2018年第9期17卷 1946-1958页

作者： xu Li-ming LIU Chan CUI Bao-ming WANG Ning ZHAO Zhuo ZHOU Li-na HUANG Kai-feng DING Jian-zhou DU Han-mei jiang Wei ZHANG Su-zhi Key Laboratory of Biology and Genetic Improvement of Maize in Southwest China Ministry of Agriculture/Maize Research InstituteSichuan Agricultural UniversityChengdu 611130P.R.China Crop Research Institute of Binzhou Binzhou 256600P.R.China

Aluminum （AI） toxicity is a major factor limiting crop production and plant growth in acid soils. The complex inheritance of AI toxicity and tolerance mechanisms in maize has uncharacterized yet. In this study, the maize inbred line 178 seedlings were treated with 200 μmol L-1 CaCI2＋0 μmol L^-1AICI3 （control） and 200μmol L^-1 CACI2＋60 IJmol L^-1 AICI3 （AI treatment） for I and 6 h, respectively. The experiment was repeated three times. Then a detailed temporal analysis of root gene expression was performed using an Agilent GeneChip with 34 715 genes, only the genes showing more than 2.0-fold difference （P〈0.01） between the control and the AI treatment maize seedlings were analyzed further. Thus, a total of 832 different expression genes, 689 significantly up-regulated and 143 down-regulated, were identified after the seedlings were treated with AI for 6 h. And 60 genes, 59 up-regulated and one down-regulated, were also detected after the seedlings were treated for 1 h. Replicated transcriptome analyses further showed that about 61% of total significantly genes could be annotated based on plant genome resources. Quantitative real-time PCR （qRT-PCT） of some selected candidate genes was used to demonstrate the microarray data, indicating significant differences between the control and AI-treated seedlings. Exposure to AI for 6 h triggered changes in the transcript levels for several genes, which were primarily related to cell wall structure and metabolism, oxidative stress response, membrane transporters, organic acid metabolism, signaling and hormones, and transcription factors, etc. After AI-treated for 1 h, differential abundance of transcripts for several transporters, kinase, and transcription factors were specifically induced. In this study, the diversity of the putative functions of these genes indicates that AI stress for a short stage induced a complex transcriptome changes in maize. These results would further help us to understand rapid and early mechanisms o

关键词： aluminum maize microarray mechanism transcriptome

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Classification and genetic counselling for a novel splicing mutation of the MLH1 intron associated with Lynch syndrome in colorectal cancer

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Gastroenterology Report 2021年第6期9卷 552-559页

作者： Ling-Ling Wang Shuang-mei Zou Lin Dong Ming Yang Dan Qi Zhao Lu Jia-Nan Chen Shi-Wen mei Zhi-xun Zhao xu Guan Zheng jiang Qian Liu Zheng Liu Xi-Shan Wang Department of Colorectal Surgery National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingP.R.China Department of Pathology National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingP.R.China

Background:Lynch-syndrome-associated cancer is caused by germline pathogenic mutations in mismatch repair *** major challenge to Lynch-syndrome screening is the interpretation of variants found by diagnostic *** study aimed to classify the MLH1 c.1989t5G>A mutation,which was previously reported as a variant of uncertain significance,to describe its clinical phenotypes and characteristics,to enable detailed genetic ***:We reviewed the database of patients with Lynch-syndrome gene detection in our hospital.A novel variant of MLH1 c.1989t5G>A identified by next-generation sequencing was further investigated in this *** staining was carried out to assess the expression of MLH1 and PMS2 protein in tumour *** silico analysis by Alamut software was used to predict the MLH1 c.1989t5G>A variant *** transcription-polymerase chain reaction and sequencing of RNA fromwhole bloodwere used to analyse the functional significance of this ***:Among affected family members in the suspected Lynch-syndrome pedigree,the patient suffered from late-stage colorectal cancer but had a good *** found the MLH1 c.1989t5G>A variant,which led to aberrant splicing and loss of MLH1 and PMS2 protein in the nuclei of tumour *** aberrant transcript was detectable and skipping of MLH1 exon 17 in carriers of MLH1 c.1989t5G>A was ***:MLH1 c.1989t5G>A was detected in a cancer family pedigree and identified as a pathological variant in patients with Lynch *** spectrumof Lynch syndrome was enriched through enhanced genetic testing and close surveillancemight help future patients who are suspected of having Lynch syndrome to obtain a definitive early diagnosis.

关键词： Lynch syndrome MLH1 genetic testing colorectal cancer genetic counselling

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Wenjing Zhitong recipe exhibits potential anti-primary dysmenorrhea properties by inhibiting COX2 and PKC signaling pathway in rats induced by estradiol benzoate and oxytocin

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Journal of Traditional Chinese Medical Sciences 2023年第3期10卷 296-309页

作者： Jialin Li Xiaoyao Liu mei jiang Yantong xu Chunguo Wang Yujie Hu Song Yang Jian Gao Weiling Wang Ting Wang School of Traditional Chinese Medicine Beijing University of Chinese MedicineBeijing 102488China Beijing Research Institute of Chinese Medicine Beijing University of Chinese MedicineBeijing 102488China Key Laboratory of Famous Doctors and Famous Prescriptions State Administration of Traditional Chinese MedicineBeijing 100020China

Objective:To explore the effect and mechanism of action of the Wenjing Zhitong recipe(WZR)in primary dysmenorrhea(PD)***:Uterine contractions were induced by estradiol benzoate and oxytocin in a PD model and WZR was *** rate of change in uterine contractility and the writhing test were used to evaluate the effects of *** serum levels of prostaglandin F_(2a)(PGF_(2a))and prostaglandin E_2(PGE_2),and the activity of cyclooxygenase-2(COX2)were detected by enzyme-linked immunosorbent assay(ELISA).The changes in phosphor-phospholipase C(pPLC/PLC),phosphor-protein kinase C(pPKC/PKC),and connexin 43(CX43)expression were detected using immunohistochemistry and western ***:WZR significantly reduced the rate of change in uterine contractility and writhing times in the PD *** treatment inhibited the enzymatic activity of COX2 and reduced the levels of PGF_(2a),PGF_(2a)/PGE2and COX2 in the PD *** also significantly reduced the expression of pPLC/PLC,pPKC/PKC and *** the inhibition of COX2 activity,caffeic acid and 1-acetyl-β-carboline were validated as the active ingredients in WZR responsible for reducing uterine ***:WZR attenuated PD by inhibiting COX2 activity,downregulating PGF_(2a)/PGE_2 expression,and inhibiting the PKC signaling pathway.

关键词： Wenjing zhitong recipe Primary dysmenorrhea Network pharmacology PGF2a PKC system

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An experimental study of extracellular signal-regulated kinase and its interventional treatments in hepatic fibrosis

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Hepatobiliary & Pancreatic Diseases International 2008年第1期7卷 51-57页

作者： jiang, Ming-De Zheng, Shu-mei xu, Hui Zeng, Wei-Zheng Zhang, Yong Sun, Hao-Ping Wang, Yun-Xia Qin, Jian-Ping Wu, Xiao-Ling Gen Hosp Chenghu Milt Command Dept Gastroenterol Chengdu 610083 Peoples R China

BACKGROUND: The pathogenesis of hepatic fibrosis and cirrhosis is still not fully understood. The extracellular signal-regulated kinase (ERK) pathway is involved in the regulation of cell proliferation and differentiation. The aim of this study was to investigate the effects of PD98059, a specific inhibitor of ERK, on the cell cycle, cell proliferation, secretion of type I collagen and expression of cyclin D1 mRNA, CDK4 mRNA and transforming growth factor-beta 1 (TGF-beta 1) mRNA in rat hepatic stellate cells (HSCs) stimulated by acetaldehyde. METHODS: Rat HSCs stimulated by acetaldehyde were incubated with PD98059 at different concentrations. The cell cycle was analysed by flow cytometry. Cell proliferation was assessed by the methyl thiazolyl tetrazolium colorimetric assay. The mRNA expression of cyclin D1, CDK4 and TGF-beta 1 was examined using the reverse transcriptase-polymerase chain reaction. Type I collagen in the culture medium was detected by enzyme-linked immunosorbent assay. RESULTS: 20, 50 and 100 mu mol/L PD98059 significantly inhibited the proliferation and provoked a G0/G1-phase arrest of acetaldehyde-induced HSCs in a dose-dependent manner. The secretion of type I collagen and the expression of cyclin D1, CDK4 and TGF-beta 1 mRNA in acetaldehyde-induced HSCs were markedly inhibited by 50 and 100 mu mol/L PD98059, respectively. CONCLUSIONS: The ERK pathway regulates the cell proliferation, secretion of type I collagen and the expression of TGF-beta 1 mRNA in rat HSCs stimulated by acetaldehyde, which is likely related to its regulative effect on the cell cycle.

关键词： extracellular signal-regulated kinase hepatic stellate cell hepatic fibrosis

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Responses of plant diversity and soil microorganism diversity to nitrogen addition in the desert steppe,China

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Journal of Arid Land 2024年第3期16卷 447-459页

作者： YE He HONG mei xu xuehui LIANG Zhiwei jiang Na TU Nare WU Zhendan College of Grassland Resources and EnvironmentInner Mongolia Agricultural UniversityInner Mongolia Key Laboratory of Soil Quality and Nutrient ResourcesHohhot 010018China Key Laboratory of Agricultural Ecological Security and Green Development at University of Inner Mongolia Hohhot 010018China

Nitrogen(N)deposition is a significant aspect of global change and poses a threat to terrestrial *** impact of plant-soil microbe relationships to N deposition has recently attracted considerable *** microorganisms have been proven to provide nutrients for specific plant growth,especially in nutrient-poor desert steppe ***,the effects of N deposition on plant-soil microbial community interactions in such ecosystems remain poorly *** investigate these effects,we conducted a 6-year N-addition field experiment in a Stipa breviflora *** steppe in Inner Mongolia Autonomous Region,*** N treatment levels(N0,N30,N50,and N100,corresponding to 0,30,50,and 100 kg N/(hm2•a),respectively)were applied to simulate atmospheric N *** results showed that N deposition did not significantly affect the aboveground biomass of desert steppe plants.N deposition did not significantly reduce the alfa-diversity of plant and microbial communities in the desert steppe,and low and mediate N additions(N30 and N50)had a promoting effect on *** variation pattern of plant Shannon index was consistent with that of the soil bacterial Chao1 index.N deposition significantly affected the beta-diversity of plants and soil bacteria,but did not significantly affect fungal *** conclusion,N deposition led to co-evolution between desert steppe plants and soil bacterial communities,while fungal communities exhibited strong stability and did not undergo significant *** findings help clarify atmospheric N deposition effects on the ecological health and function of the desert steppe.

关键词： soil microorganisms plant-microbial community interaction plant diversity nitrogen deposition desert steppe

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Altered integrity of hepatocyte tight junctions in rats with triptolide-induced cholestasis

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Chinese Journal of Natural Medicines 2021年第3期19卷 188-194页

作者： HUANG Shan LIU Li mei Hui-Fang ZHANG Qian-Wen ZHANG Xi xu Xiao-Ting WANG Xin-Zhi HUANG Xin WANG Tao jiang Zhen-Zhou ZHANG Lu-Yong SUN Li-Xin jiangsu Key Laboratory of Drug Screening China Pharmaceutical UniversityNanjing 210009China Center for Drug Research and Development Guangdong Pharmaceutical UniversityGuangzhou 510006China jiangsu Center for Pharmacodynamics Research and Evaluation China Pharmaceutical UniversityNanjing 210009China Changde Vocational Technology College Changde 415000China

Triptolide(TP),an active component of Tripterygium wilfordii Hook.f.(TWHF),has been widely used for centuries as a traditional Chinese ***,the clinical application of TP has been restricted due to multitarget toxicity,such as *** this study,28 days of oral TP administration(100,200,or 400μg·kg^(-1)·d^(-1))induced the occurrence of cholestasis in female Wistar rats,as evidenced by increased serum levels ofγ-glutamyl transpeptidase(γ-GGT),alkaline phosphatase(ALP)and hepatic total bile acids(TBAs).In addition,the heptocyte polarity associated with the strcture of tight junctions(TJs)was disrupted in both rats and sandwich-cultured primary *** revealed decreased expression of the TJ-associated proteins occludin,claudin^(-1),and zonula occludens protein(ZO^(-1)),and downregulated m RNA levels of these TJs was also detected by real-time *** immunofluorescence analysis showed abnormal subcellular localization of occludin,claudin^(-1) and ZO^(-1),which was also confirmed by transmission electron ***,the concentration of FITC-dextran,a marker of paracellular penetration,was found to increase rapidly in bile increased rapidly(within 6 minutes)after treatment with TP,which indicated the functional impairment of *** together,these results suggest that the administration of TP for 28 consecutive days to rats could induce cholestatic injury in the liver,and the increased paracellular permeability might play an important role in these pathological changes.

关键词： Triptolide Cholestatic injury Tight junction Sandwich-cultured primary hepatocytes

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Chloromycetin resistance of clinically isolated E coli is conversed by using EGS technique to repress the Chloromycetin acetyl transferase

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World Journal of Gastroenterology 2005年第46期11卷 7368-7373页

作者： mei-Ying Gao Chuan-Rui xu RU Chen Shou-Gui Liu jiang-Nan Feng Department of Infectious Diseases Tongji Hospital Tongji JVledical CollegeHuazhong University of Science and Technology Wuhan 430030Hubei Province China School of Pharmacy Tongji Medical CollegeHuazhong University of Science and Technology Wuhan 430030Hubei Province China Laboratory of Immunology Wuhan Bioproduct Institute of Ministry of Public Health Wuhan 430060 Hubei Province China

AIM： To explore the possibility of repression of chloromycetin （Cm） acyl transferase by using external guided sequence （EGS） in order to converse the clinical E coli isolates from Cm- resistant to Cm- sensitive. METHODS： EGS directed against chloromycetin acetyl transferase gene （cat） was cloned to vector pEGFP-C1 which contains the kanamycin （Kin） resistance gene. The recombinant plasmid pEGFP-C1＋EGScatl＋cat2 was constructed and the blank vector without EGS fragment was used as control plasmids. By using the CaCl2 transformation method, the recombinant plasmids were introduced into the clinically isolated Cm resistant but Km sensitive E coli strains. Transformants were screened on LB agar plates containing Kin. Extraction of plasmids and PCR were applied to identify the positive clones. The growth curve of EGS transformed bacteria cultured in broth with Cm resistance was determined by using spectrophotometer at A600. Drug sensitivity was tested in solid culture containing Cm by using KB method. RESULTS： Transformation studies were carried out on 16 clinically isolated Cm-resistant （250 μg/mL of Cm） E colistrains by using pEGFP-C1-EGScatlcat2 recombinant plasmid. Transformants were screened on LB-agar plates containing Km after the transformation using EGS. Of the 16 tested strains, 4 strains were transformed successfully. Transformants with EGS plasmid showed growth inhibition when grown in liquid broth culture containing 200 μg/mL of Cm. In drug sensitivity test, these strains were sensitive to Cm on LB-agar plates containing 200 μg/mL of Cm. Extraction of plasmids and PCR amplification showed the existence of EGS plasmids in these four transformed strains. These results indicated that the Cat of the four clinical isolates had been suppressed and the four strains were converted to Cm sensitive ones. CONCLUSION： The EGS directed against Cat is able to inhibit the expression of Cat, and hence convert Cm- resistant bacteria to Cm-sensitive ones. Thus, the EGS h

关键词： External guide sequence Drug-resistant bacteria Conversion of drug resistance

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