Wenjing Zhitong recipe exhibits potential anti-primary dysmenorrhea properties by inhibiting COX2 and PKC signaling pathway in rats induced by estradiol benzoate and oxytocin

作     者：Jialin Li Xiaoyao Liu Mei Jiang Yantong Xu Chunguo Wang Yujie Hu Song Yang Jian Gao Weiling Wang Ting Wang Jialin Li;Xiaoyao Liu;Mei Jiang;Yantong Xu;Chunguo Wang;Yujie Hu;Song Yang;Jian Gao;Weiling Wang;Ting Wang

作者机构：School of Traditional Chinese MedicineBeijing University of Chinese MedicineBeijing 102488China Beijing Research Institute of Chinese MedicineBeijing University of Chinese MedicineBeijing 102488China Key Laboratory of Famous Doctors and Famous PrescriptionsState Administration of Traditional Chinese MedicineBeijing 100020China 

出 版 物：《Journal of Traditional Chinese Medical Sciences》 (中医科学杂志（英文）)

年 卷 期：2023年第10卷第3期

页      面：296-309页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by Beijing University of Chinese Medicine(1000061224003) National Natural Science Foundation of China(81903766,81900603,82104440) 

主　　题：Wenjing zhitong recipe Primary dysmenorrhea Network pharmacology PGF2a PKC system 

摘      要：Objective:To explore the effect and mechanism of action of the Wenjing Zhitong recipe(WZR)in primary dysmenorrhea(PD)***:Uterine contractions were induced by estradiol benzoate and oxytocin in a PD model and WZR was *** rate of change in uterine contractility and the writhing test were used to evaluate the effects of *** serum levels of prostaglandin F_(2a)(PGF_(2a))and prostaglandin E_2(PGE_2),and the activity of cyclooxygenase-2(COX2)were detected by enzyme-linked immunosorbent assay(ELISA).The changes in phosphor-phospholipase C(pPLC/PLC),phosphor-protein kinase C(pPKC/PKC),and connexin 43(CX43)expression were detected using immunohistochemistry and western ***:WZR significantly reduced the rate of change in uterine contractility and writhing times in the PD *** treatment inhibited the enzymatic activity of COX2 and reduced the levels of PGF_(2a),PGF_(2a)/PGE2and COX2 in the PD *** also significantly reduced the expression of pPLC/PLC,pPKC/PKC and *** the inhibition of COX2 activity,caffeic acid and 1-acetyl-β-carboline were validated as the active ingredients in WZR responsible for reducing uterine ***:WZR attenuated PD by inhibiting COX2 activity,downregulating PGF_(2a)/PGE_2 expression,and inhibiting the PKC signaling pathway.