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Ultra-high spatio-temporal resolution imaging with parallel acquisition-readout structured illumination microscopy(PAR-SIM)

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Light(Science & Applications) 2024年第6期13卷 1140-1158页

作者： xinzhu xu Wenyi Wang Liang Qiao Yunzhe Fu Xichuan Ge Kun Zhao Karl Zhanghao Meiling Guan Xin Chen Meiqi Li Dayong Jin Peng Xi Department of Biomedical Engineering College of Future TechnologyPeking UniversityBeijing100871China Wallace H.Coulter Dept.of Biomedical Engineering Georgia Institute of Technology and Emory UniversityAtlanta30332GAUSA National Biomedical Imaging Center Peking UniversityBeijing100871China Department of Biomedical Engineering College of EngineeringSouthern University of Science and TechnologyShenzhen518055GuangdongChina Airy Technologies Co. Ltd.Beijing100086China Eastern Institute for Advanced Study Eastern Institute of TechnologyNingboZhejiang315200China School of Life Science Peking UniversityBeijing100871China Institute for Biomedical Materials and Devices(IBMD) Faculty of ScienceUniversity of Technology SydneySydneyNSW2007Australia

Structured illumination microscopy(SIM)has emerged as a promising super-resolution fluorescence imaging technique,offering diverse configurations and computational strategies to mitigate phototoxicity during real-time imaging of biological *** efforts to enhance system frame rates have concentrated on processing algorithms,like rolling reconstruction or reduced frame reconstruction,or on investments in costly sCMOS cameras with accelerated row readout *** this article,we introduce an approach to elevate SIM frame rates and region of interest(ROI)coverage at the hardware level,without necessitating an upsurge in camera expenses or intricate ***,parallel acquisition-readout SIM(PAR-SIM)achieves the highest imaging speed for fluorescence imaging at currently available detector *** using the full frame-width of the detector through synchronizing the pattern generation and image exposure-readout process,we have achieved a fundamentally stupendous information spatial-temporal flux of 132.9 MPixels·s^(−1),9.6-fold that of the latest techniques,with the lowest SNR of−2.11 dB and 100 nm ***-SIM demonstrates its proficiency in successfully reconstructing diverse cellular organelles in dual excitations,even under conditions of low signal due to ultra-short exposure ***,mitochondrial dynamic tubulation and ongoing membrane fusion processes have been captured in live COS-7 cell,recorded with PAR-SIM at an impressive 408 *** posit that this novel parallel exposure-readout mode not only augments SIM pattern modulation for superior frame rates but also holds the potential to benefit other complex imaging systems with a strategic controlling approach.

关键词： hardware illumination resolution

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某核电厂蒸汽发生器排污净化系统泵出口管道泄漏原因

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腐蚀与防护 2024年第6期45卷 119-125页

作者：韩辉许新竹江苏核电有限公司连云港222042

国内某机组运行15 a后,蒸汽发生器排污净化系统泵出口管道发生泄漏。采用超声波测厚、化学成分分析、金相检验、扫描电镜观察、能谱分析及流场模拟计算等方法,分析了管道泄漏的原因。结果表明:低温流动加速腐蚀(FAC)导致管壁减薄,最终... 详细信息

国内某机组运行15 a后,蒸汽发生器排污净化系统泵出口管道发生泄漏。采用超声波测厚、化学成分分析、金相检验、扫描电镜观察、能谱分析及流场模拟计算等方法,分析了管道泄漏的原因。结果表明:低温流动加速腐蚀(FAC)导致管壁减薄,最终导致管道泄漏,管件材质、几何形状,介质pH、温度、溶解氧含量及流速等是发生FAC的重要因素。

关键词：管道泄漏流动加速腐蚀(FAC) 温度介质

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Rare nanoparticles shine colors with low-power STED

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Light(Science & Applications) 2022年第7期11卷 1324-1326页

作者： xinzhu xu Peng Xi College of Future Technology Peking UniversityBeijingChina

The effect of cascade amplified depletion in lanthanide upconversion systems boosts their own emission bands inhibition,which facilitates multi-color nanoscopy with only one pair of low-power NIR CW lasers.

关键词： Rare lanthanide bands

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中医院校基于雨课堂的医学免疫学与病原生物学混合式教学实践

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中国中医药现代远程教育 2024年第16期22卷 37-40页

作者：雷萍徐铭王建光董墨思许欣竹韩晓伟辽宁中医药大学免疫与病原生物学教研室辽宁沈阳110847

构建基于雨课堂的医学免疫学与病原生物学混合式教学模式。进行线上资源及线下教学资料的筛选,并从课前、课中和课后3个环节进行教学设计并实施。结果显示:2019级中医学专业学生(混合式教学)在期末考试中取得了比2017级中医学专业学生(... 详细信息

构建基于雨课堂的医学免疫学与病原生物学混合式教学模式。进行线上资源及线下教学资料的筛选,并从课前、课中和课后3个环节进行教学设计并实施。结果显示:2019级中医学专业学生(混合式教学)在期末考试中取得了比2017级中医学专业学生(单纯线下教学)以及2018级中医学专业学生(单纯线上教学)更好的成绩。并且,通过雨课堂把课程思政有效融入医学免疫学与病原生物学,起到了润物细无声的育人效果,可为培养新时代的中医药人才提供一定的借鉴方法。

关键词：雨课堂混合式教学课程思政医学免疫学与病原生物学

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Two-photon MINFLUX with doubled localization precision

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eLight 2022年第1期2卷 58-67页

作者： Kun Zhao xinzhu xu Wei Ren Dayong Jin Peng Xi Department of Biomedical Engineering College of Future TechnologyPeking UniversityBeijing 100871China Wallace H.Coulter Department of Biomedical Engineering Georgia Institute of Technology and Emory UniversityAtlanta30332 GeorgiaUSA UTS‑SUStech Joint Research Centre for Biomedical Materials&Devices Department of Biomedical EngineeringCollege of EngineeringSouthern University of Science and TechnologyShenzhenGuangdong 518055China National Biomedical Imaging Center Peking UniversityBeijing 100871China

Achieving localization with molecular precision has been of great interest for extending fluorescence microscopy to *** pioneers this transition through point spread function(PSF)engineering,yet its performance is primarily limited by the signal-to-background *** we demonstrate theoretically that two-photon MINFLUX(2p-MINFLUX)could double its localization precision through PSF engineering by nonlinear ***ér-Rao Bound(CRB)is studied as the maximum localization precision,and CRB of two-photon MINFLUX is halved compared to single-photon MINFLUX(1p-MINFLUX)in all three ***,in order to achieve same localization precision with 1p-MINFLUX,2p-MINFLUX requires only 1/4 of fluorescence *** simultaneous two-photon excitation of multiple fluorophore species,2p-MINFLUX may have the potential for registration-free nanoscopy and multicolor tracking.

关键词： Super-resolution microscopy Single-molecule localization MINFLUX Two-photon microscopy

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Raster-scanning Donut simplifies MINFLUX and provides alternative implement on other scanning-based microscopes

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Light(Science & Applications) 2022年第10期11卷 2271-2275页

作者： xinzhu xu Shu Jia Peng Xi Department of Biomedical Engineering College of Future TechnologyPeking UniversityBeijing 100871China Wallace H.Coulter Department of Biomedical Engineering Georgia Institute of Technology and Emory UniversityAtlantaGeorgia 30332USA UTS-SUStech Joint Research Centre for Bio-medical Materials&Devices Department of Biomedical EngineeringCollege of EngineeringSouthern University of Science and TechnologyShenzhenGuangdong 518055China National Biomedical Imaging Center Peking UniversityBeijing 100871China

A donut excitation moves around a single molecule with a zigzag configuration lattice by *** a method implemented in scanning fluorescence microscopy simplifies the conventional MINFLUX *** of hollow zero-intensity excitation,single-pixel detection,time-correlated single photon counting,and drift stabilization,the system achieves localization precision and resolution very close to conventional MINFLUX theoretically and *** averaged high-SNR reference,and pixel-registered intensity from a single molecule is essential to reconstruct localization in maximum likelihood *** performance reaching nearly conventional MINFLUX's,the proposed raster-scanning MINFLUX can inspire researchers expertized in STED or confocal setup to quickly transform to MINFLUX and develop for further exploring on bio-specimens or optical applications.

关键词： scanning excitation stabilization

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Frequency-domain diagonal extension imaging

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Advanced Photonics 2020年第3期2卷 61-68页

作者： Shan Jiang Meiling Guan Jiamin Wu Guocheng Fang xinzhu xu Dayong Jin Zhen Liu Kebin Shi Fan Bai Shu Wang Peng Xi Peking University College of EngineeringDepartment of Biomedical EngineeringBeijingChina Southern University of Science and Technology China Department of Biomedical EngineeringShenzhenGuangdongChina Beijing Institute of Collaborative Innovation(BICI) BeijingChina Tsinghua University Department of AutomationBeijingChina University of Technology Sydney Faculty of ScienceInstitute for Biomedical Materials&Devices(IBMD)UltimoAustralia Peking University School of PhysicsBeijingChina Peking University School of Life SciencesBiodynamic Optical Imaging Center(BIOPIC)BeijingChina Peking University People’s Hospital Breast Center BeijingChina

The pixel size of a charge-coupled device(CCD)camera plays a major role in the image resolution,and the square pixels are attributed to the physical anisotropy of the sampling *** synthesize the high sampling frequency directions from multiple frames acquired with different angles to enhance the resolution by 1.4×over conventional CCD orthogonal *** directly demonstrate the improvement of frequency-domain diagonal extension(FDDE)microscopy,lens-free microscopy is used,as its resolution is dominantly determined by the pixel *** demonstrate the resolution enhancement with a mouse skin histological specimen and a clinical blood smear ***,FDDE is extended to lens-based photography with an ISO 12233 resolution *** method paves a new way for enhancing the image resolution for a variety of imaging techniques in which the resolution is primarily limited by the sampling pixel size,for example,microscopy,photography,and spectroscopy.

关键词： frequency domain diagonal sampling super-resolution

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Insights into spinal muscular atrophy from molecular biomarkers

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Neural Regeneration Research 2025年第7期20卷 1849-1863页

作者： Xiaodong Xing xinzhu Liu Xiandeng Li Mi Li Xian Wu Xiaohui Huang Ajing xu Yan Liu Jian Zhang Department of Clinical Pharmacy Xinhua HospitalShanghai Jiao Tong University School of MedicineShanghaiChina College of Pharmacy Chongqing Medical UniversityChongqingChina

Spinal muscular atrophy is a devastating motor neuron disease characterized by severe cases of fatal muscle *** is one of the most common genetic causes of mortality among infants aged less than 2 *** research is currently receiving more attention,and new candidate biomarkers are constantly being *** review initially discusses the evaluation methods commonly used in clinical practice while briefly outlining their respective pros and *** also describe recent advancements in research and the clinical significance of molecular biomarkers for spinal muscular atrophy,which are classified as either specific or non-specific *** review provides new insights into the pathogenesis of spinal muscular atrophy,the mechanism of biomarkers in response to drug-modified therapies,the selection of biomarker candidates,and would promote the development of future ***,the successful utilization of biomarkers may facilitate the implementation of gene-targeting treatments for patients with spinal muscular atrophy.

关键词： biomarkers disease progression gene-targeting therapy neurofilaments Nusinersen spinal muscular atrophy(SMA) survival motor neuron therapeutic evaluation treatment outcomes

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Mitophagy in the Skeletal Muscle Is Suppressed in Spleen Qi Deficiency

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Chinese Medicine 2019年第1期10卷 11-18页

作者： Dan Ma Wenjun Liu Lu Wang xinzhu xu Lingzhi Wang Huaxin Yu xudong Liu Huihui Liu Deshan Wang Dehong Shan School of Basic Medicine Liaoning University of Traditional Chinese Medicine Shenyang China Teaching and Experimental Center Liaoning University of Traditional Chinese Medicine Shenyang China

Objective: Mitochondrial impairment in the skeletal muscle contributes to useless of limbs in spleen qi deficiency;however the genesis of such impairment is not clear. Herein, PTEN-induced putative kinase 1 (PINK1)-Parkin pathway and mitophagy were studied to explore the machinery of mitochondrial impairment. Methods: 16 male SD rats were randomly divided in the control group and spleen qi deficiency group (model group);transmission electron microscope was used to observe mitochondrial morphology;mitochondrial oxidative phosphorylation was assessed by testing mitochondrial membrane potential (MMP) and levels of ATP and ROS;western blot was used to analyze expressions of PINK1, Parkin, microtubule-associated protein 1 light chain 3-II (LC3-II) and p62. Results: Compared with those in the control group, mitochondria became small, less and scattered, MMP and the ATP level were reduced, the ROS level was elevated, PINK1 expression was decreased, p62 expression was increased, but Parkin and LC3-II expressions were not altered, in the model group. Conclusions: Suppression of mitophagy might be related to the mitochondrial damage in the skeletal muscle when spleen qi deficiency develops.

关键词： Spleen Qi Deficiency Mitophagy PINK1 Parkin LC3 p62 Muscle

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某核电厂核级设备法兰泄漏监测管开裂原因

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腐蚀与防护 2022年第6期43卷 97-102页

作者：杨兴旺马若群许新竹江苏核电有限公司连云港222042 生态环境部核与辐射安全中心北京100082

在国内某核电厂运行1 a后的大修期间,发现核级设备法兰泄漏监测管发生开裂.采用化学成分分析、金相检验、拉伸性能测试、扫描电镜及能谱分析等方法,分析了泄漏监测管开裂的原因.结果表明:该泄漏监测管的开裂形式为应力腐蚀开裂;泄漏监... 详细信息

在国内某核电厂运行1 a后的大修期间,发现核级设备法兰泄漏监测管发生开裂.采用化学成分分析、金相检验、拉伸性能测试、扫描电镜及能谱分析等方法,分析了泄漏监测管开裂的原因.结果表明:该泄漏监测管的开裂形式为应力腐蚀开裂;泄漏监测管在安装阶段冲洗不彻底,导致氯离子残留在管内,在氯离子聚集处首先形成腐蚀坑,在应力及腐蚀性介质的共同作用下,该处产生裂纹,裂纹不断扩展,最终导致泄漏监测管发生开裂.

关键词：法兰泄漏监测管氯离子腐蚀坑应力腐蚀开裂

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