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Numerical study of opposed zero-net-mass-flow jet-induced erythrocyte mechanoporation

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Applied Mathematics and Mechanics(English Edition) 2022年第11期43卷 1763-1776页

作者： xinyue liu Jinfang AI Jun XIE Guohui HU Shanghai Institute of Applied Mathematics and Mechanics School of Mechanics and Engineering ScienceShanghai Key Laboratory of Mechanics in Energy EngineeringCollaborative Innovation Center for the Marine Artificial IntelligentShanghai UniversityShanghai 200072China Zhejiang Lab Hangzhou 311121China

With the advantages of biosafety and efficiency,increasing attention has been paid to the devices for gene and macromolecular drug delivery based on *** transient pore formation on the cell membrane allows cargo transportation when the membrane areal strain is beyond the critical pore value and below the lysis tension *** on this principle,we propose a method to apply the proper fluid stress on cells moving in a microchannel under the action of zero-net-mass-flux(ZNMF)*** this study,an immersed finite element method(IFEM)is adopted to simulate the interaction between the cells and the fluid fields so as to investigate the cell movement and deformation in this mechanoporation *** evaluate the efficiency of the cargo delivery,a pore integral is defined as the mean pore rate when the cell passes through the jet *** analyzing the effects of the parameters,including the pressure gradient along the microchannel,the jet amplitude,and the jet frequency,on the pore integrals,a group of optimized parameters for cargo delivery efficiency are ***,the stability and safety of this system are analyzed in *** results are helpful in designing the mechanoporation devices and improving their efficiency of drug delivery.

关键词： drug delivery membrane mechanoporation zero-net-mass-flux(ZNMF)jet immersed finite element method(IFEM)

Numerical study of clathrin-mediated endocytosis of nanoparticles by cells under tension

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Acta Mechanica Sinica 2019年第3期35卷 691-701页

作者： xinyue liu Hongwei Yang Yunqiao liu Xiaobo Gong Huaxiong Huang Key Laboratory of Hydrodynamics (Ministry of Education) Department of Engineering MechanicsSchool of Naval ArchitectureOcean and Civil EngineeringShanghai Jiao Tong UniversityShanghai 200240China Department of Aeronautics and Astronautics Fudan UniversityShanghai 200433China Department of Mathematics and Statistics York UniversityTorontoON M3J 1P3Canada

In this study, a three-dimensional mathematical model was used to study the contribution of clathrins during the process of cellular uptake of spherical nanoparticles under different membrane tensions. The clathrin-coated pit (CCP) that forms around the inward budding of the cell membrane was modeled as a vesicle with bending rigidity. An optimization algorithm was proposed for minimizing the total energy of the system, which comprises the deforming nanoparticle, receptor-ligand bonds, cell membrane, and CCP, in which way, the profile of the system is acquired. The results showed that the CCP enable full wrapping of the nanoparticles at various membrane tensions. When the cell membrane tension increases, the total deformation energy also increases, but the ratio of CCP bending to the minimum value of the total energy of the system decreases. The results also showed that the diameter of the endocytic vesicles determined by the competition between the stretching of the cell membrane and confinement of the coated pits are much larger than the nanoparticles, which is quit different as the results in passive endocytosis that is not facilitated by the CCPs. The present results indicate that variations of tension on cell membranes constitutes a biophysical marker for understanding the size distribution of CCPs observed in experiments. The present results also suggest that the early abortion of endocytosis is related to that the receptor-ligand bonds cannot generate adequate force to wrap the nanoparticles into the cell membrane before the clathrins respond to support the endocytic vesicles. Correspondingly, late abortion may relate to the inability of CCPs to confine the nanoparticles until the occurrence of the necking stage of endocytosis.

关键词： Endocytosis Minimum energy method Membrane tension Clathrin-coated pit Receptor-ligand bond

A Numerical Study of Passive Receptor-Mediated Endocytosis of Nanoparticles:The Effect of Mechanical Properties

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Computer Modeling in Engineering & Sciences 2018年第8期116卷 281-300页

作者： xinyue liu Yunqiao liu Xiaobo Gong Huaxiong Huang Key Laboratory of Hydrodynamics(Ministry of Education) Department of Engineering MechanicSchool of Naval ArchitectureOcean and Civil EngineeringShanghai Jiao Tong UniversityShanghai200240China. Department of Mathematics and Statistics York UniversityTorontoOntarioM3J1P3Canada.

In this work,a three-dimensional axisymmetric model with nanoparticle,receptor-ligand bonds and cell membrane as a system was used to study the quasi-static receptor-mediated endocytosis process of spherical nanoparticles in drug *** minimization of the system energy function was carried out numerically,and the deformations of nanoparticle,receptor-ligand bonds and cell membrane were *** show that passive endocytosis may fail due to the rupture of receptor-ligand bonds during the wrapping process,and the size and rigidity of nanoparticles affect the total deformation energy and the terminal wrapping *** results suggest that,in addition to the energy requirement,the success of passive endocytosis also depends on the maximum strength of the receptor-ligand bonds.

关键词： Receptor-mediated endocytosis nanoparticle uptake optimization method receptor-ligand bonds drug delivery

GAMYB transcription factor LoMYB65 from lily plays a vital role in pollen development

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Horticultural Plant Journal 2024年第1期10卷 223-238页

作者： xinyue liu Ling He Ze Wu Nianjun Teng Key Laboratory of Landscaping Agriculture Ministry of Agriculture and Rural AffairsCollege of HorticultureNanjing Agricultural UniversityNanjingJiangsu 210095China Key Laboratory of Biology of Ornamental Plants in East China National Forestry and Grassland AdministrationCollege of HorticultureNanjing Agricultural UniversityNanjingJiangsu 210095China Jiangsu Graduate Workstation of Nanjing Agricultural University and Nanjing Oriole Island Modern Agricultural Development Co. Ltd.NanjingJiangsu 210043China

Lily(Lilium spp.)is an important horticultural crop,but its use is limited due to serious pollen contamination *** are many studies on pollen development in model plants,but few on flower crops such as ***(GA)is a large class of hormones and plays an important role in plant vegetative growth and reproductive *** is a group of the R2R3-MYB family upregulated by gibberellin,and plays an important role in anther ***,we isolated a novel GAMYB,named LoMYB65,from lily,which was closely related to the AtMYB65 and AtMYB33 in *** quantitative PCR results showed that LoMYB65 was mainly expressed in lily ***65 could be activated by 288μmol·L^(-1)GA3treatment and the LoMYB65 protein was located in the nucleus and cytoplasm,and had transactivation in yeast and tobacco leaf *** conserved motif within 226 amino acids of the C-terminal of LoMYB65 contributed to its *** of LoMYB65 caused dwarf phenotype,unnormal tapetum development,less seeds of siliques in transgenic Arabidopsis plants,the transgenic plants showed partly male ***,silencing of LoMYB65 with VIGS(Virus Induced Gene Silencing)in lily anthers caused unnormal pollen development and reduced the pollen *** of LoMYB65 in Arabidopsis and silencing of LoMYB65 in lily resulted in decreased pollen counts,so we speculate that LoMYB65 may be ***,these findings suggest that LoMYB65 may play an important role in anther development and pollen formation in ***65 may provide a useful candidate gene for pollenless breeding of lily.

关键词： Lily Anther development Pollen pollution GAMYB VIGS

Prediction of single cell mechanical properties in microchannels based on deep learning

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Applied Mathematics and Mechanics(English Edition) 2024年第11期45卷 1857-1874页

作者： Jiajie GONG xinyue liu Yancong ZHANG Fengping ZHU Guohui HU Shanghai Institute of Applied Mathematics and Mechanics School of Mechanics and Engineering ScienceShanghai Frontier Science Center of MechanoinformaticsShanghai Key Laboratory of Mechanics in Energy EngineeringShanghai UniversityShanghai200072China Shanghai Institute of Aircraft Mechanics and Control Shanghai200092China Department of Neurosurgery Huashan HospitalShanghai Medical CollegeFudan UniversityShanghai200040China

Traditional methods for measuring single-cell mechanical characteristics face several challenges,including lengthy measurement times,low throughput,and a requirement for advanced technical *** overcome these challenges,a novel machine learning(ML)approach is implemented based on the convolutional neural networks(CNNs),aiming at predicting cells'elastic modulus and constitutive equations from their deformations while passing through micro-constriction *** the present study,the computational fluid dynamics technology is used to generate a dataset within the range of the cell elastic modulus,incorporating three widely-used constitutive models that characterize the cellular mechanical behavior,i.e.,the Mooney-Rivlin(M-R),Neo-Hookean(N-H),and Kelvin-Voigt(K-V)*** this dataset,a multi-input convolutional neural network(MI-CNN)algorithm is developed by incorporating cellular deformation data as well as the time and positional *** approach accurately predicts the cell elastic modulus,with a coefficient of determination R^(2)of 0.999,a root mean square error of 0.218,and a mean absolute percentage error of 1.089%.The model consistently achieves high-precision predictions of the cellular elastic modulus with a maximum R^(2)of 0.99,even when the stochastic noise is added to the simulated *** significant feature of the present model is that it has the ability to effectively classify the three types of constitutive equations we *** model accurately and reliably predicts single-cell mechanical properties,showcasing a robust ability to *** demonstrate that incorporating deformation features at multiple time points can enhance the algorithm's accuracy and *** algorithm presents a possibility for high-throughput,highly automated,real-time,and precise characterization of single-cell mechanical properties.

关键词： cell deformation single-cell mechanics machine learning(ML) constitutive law convolutional neural network(CNN)

A numerical method to predict the membrane tension distribution of spreading cells based on the reconstruction of focal adhesions

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Science China(Physics,Mechanics & Astronomy) 2022年第6期65卷 88-100页

作者： xinyue liu Keni-chi Tsubota Yi Yu Wang Xi XiaoBo Gong Shanghai Institute of Applied Mathematics and Mechanics School of Mechanics and Engineering ScienceShanghai Key Laboratory of Mechanics in Energy EngineeringShanghai UniversityShanghai 200072China Key Laboratory of Hydrodynamics(Ministry of Education) Department of Engineering MechanicsSchool of Naval ArchitectureOcean and Civil EngineeringShanghai Jiao Tong UniversityShanghai 200240China Department of Mechanical Engineering Chiba UniversityChiba 263-8522Japan Department of Aeronautics and Astronautics Fudan UniversityShanghai 200433China Mechanobiology Institute National University of SingaporeSingapore 117411Singapore Institut Jacques Monod UniversitéParis CitéCentre National de la Recherche ScientifiqueParis F-75013France Institute of Mechanobiology and Biomedical Engineering School of Life Science and TechnologyShanghai Jiao Tong UniversityShanghai 200240China

Changes in membrane tension significantly affect the physiological functions of cells in various ***,directly measuring the spatial distribution of membrane tension remains an ongoing *** this study,an algorithm is proposed to determine the membrane tension inversely by executing a particle-based method and searching for the minimum deformation energy based on the cell images and focal adhesions.A standard spreading cell model is established using 3D reconstructions with images from structured illumination microscopy as the reference cell *** membrane tension distribution,forces across focal adhesions,and profile of the spread cell are obtained using this method,until the cell deformation energy function optimization *** and quantitative comparisons with previous experimental results validated the reliability of this *** results show that in the standard spreading cell model,the membrane tension decreases from the bottom to the top of the *** method can be applied to predict the membrane tension distribution of cells freely spreading into different shapes,which could improve the quantitative analysis of cellular membrane tension in various studies for cell mechanics.

关键词： cell spread membrane tension distribution particle-based method optimization method 3D reconstructions

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Acoustic emissions evaluation of the dynamic splitting tensile properties of steel fiber reinforced concrete under freeze-thaw cycling

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Frontiers of Structural and Civil Engineering 2023年第9期17卷 1341-1356页

作者： Hua ZHANG xinyue liu Lingyu BAI Shanshan JI Luoyu PAN Xuechen LI College of Civil and Transportation Engineering Hohai UniversityNanjing 210098China

This study empirically investigated the influence of freeze-thaw cycling on the dynamic splitting tensile properties of steel fiber reinforced concrete(SFRC).Brazilian disc splitting tests were conducted using four loading rates(0.002,0.02,0.2,and 2 mm/s)on specimens with four steel fiber contents(0%,0.6%,1.2%,and 1.8%)subjected to 0 and 50 freeze-thaw *** dynamic splitting tensile damage characteristics were evaluated using acoustic emission(AE)parameter analysis and Fourier transform spectral *** results quantified using the freeze-thaw damage factor defined in this paper indicate that the degree of damage to SFRC caused by freeze-thaw cycling was aggravated with increasing loading rate but mitigated by increasing fiber *** percentage of low-frequency AE signals produced by the SFRC specimens during loading decreased with increasing loading rate,whereas that of high-frequency AE signals ***-thaw action had little effect on the crack types observed during the early and middle stages of the loading process;however,the primary crack type observed during the later stage of loading changed from shear to tensile after the SFRC specimens were subjected to freeze-thaw ***,the results of this study indicate that the freeze-thaw damage to SFRC reduces AE signal activity at low frequencies.

关键词： steel fiber reinforced concrete freeze-thaw cycling Brazilian disc splitting test acoustic emission technique dynamic splitting tensile acoustic emission properties

Design and test of the anti-skid system for self-propelled high-stem crop sprayers

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International Journal of Agricultural and Biological Engineering 2023年第6期16卷 20-27页

作者： Haojun Wen xinyue liu Zhetian Yi Zhongxiang Li School of Mechanical and Electrical Engineering Shihezi UniversityShihezi 832003XinjiangChina Northwest Key Laboratory of Agricultural Equipment Ministry of Agriculture and Rural AffairsShihezi 832003XinjiangChina

Aiming at the problem of skidding in the operation of a self-propelled high-stem crop sprayer due to uneven road surface and uneven distribution of soil cohesive force,This study proposed a four-wheel drive hydraulic anti-skid control system based on RTM *** theoretical analysis of control components such as the RTM valve and steering cylinder,the control model of the anti-skid drive system of the spray machine is established,and the simulation test is carried out in Matlab/*** simulation results show that the slip rate of the anti-skid drive system based on the PID control strategy is controlled below 0.05,and the RTM valve keeps the driving pressure of the system basically *** order to verify the reliability of the simulation results,the designed drive anti-skid control system was carried out on a spray machine for the field *** experimental results show that the slip rate of the drive anti-skid system decreases from 86.7%to 1.25%when the anti-skid function is turned on,indicating that the designed drive anti-skid system has good anti-skid performance.

关键词： self-propelled sprayer high-stem crops drive anti-skid PID control Matlab Simulink

Performance measurement of adaptive optics system based on Strehl ratio

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The Journal of China Universities of Posts and Telecommunications 2016年第3期23卷 94-100页

作者： Wang Liang Chen Tao Lin Xudong Wei Peifeng liu xinyue Jia Jianlu Changchun Institute of Optics Fine Mechanics and Physics Chinese Academy of Sciences Changchtm 130033 China University of Chinese Academy of Sciences Beijing 100049 China

In this article, a method for measuring the performance of adaptive optics （AO） systems is designed and validated by experiments. The Strehl ratio （SR） which is based on the target images is used to evaluate the performance quantitatively because it relates to the effect of AO correction directly. In the calculation of the SR, to avoid energy scaling in the diffraction-limited point spread function, an algorithm based on the integral of the optical transfer function （OTF） is proposed. Then, a 97-element AO system is established to validate this method, and a white-light fiber source is used as a point-like target. To simulate the practical conditions which influence the performance of the AO system, targets of different brightness are simulated in terms of different signal-to-noise ratios （SNRs） of the Shack-Hartmann （SH）, and atmospheric turbulence is simulated in terms of the Fried＇s coherence length and the Greenwood＇s frequency. Finally, two experiments are conducted in which the SR of different simulated conditions are measured. The results of the experiments show that for a moderate SNR of SH the experimenting AO system is capable of closed-loop wavefront correction when the Fried＇s coherence length is larger than 5cm and the Greenwood＇s frequency is lower than 60 Hz. The results also show that the performance of AO is susceptible to the SNR of SH. The experiments validates the effectiveness of this method.

关键词： adaptive optics performance measurement Strehl ratio turbulence simulator