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N-酰基高丝氨酸内酯降解菌的筛选及其群体感应淬灭活性研究

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食品工业科技 2025年第2期46卷 159-166页

作者：檀茜倩王晓晴崔方超王当丰吕欣然李学鹏励建荣渤海大学食品科学与工程学院/海洋研究院天津科技大学食品科学与工程学院

为了研究土壤中具有群体感应淬灭作用的细菌类型以及其群体感应淬灭活性，本研究对土壤中可降解蜂房哈夫尼菌（Hafnia alvei）所产N-酰基高丝氨酸内酯的菌株进行了筛选、分离和鉴定，对群体感应淬灭菌的特性以及其对蜂房哈夫尼菌生物被... 详细信息

为了研究土壤中具有群体感应淬灭作用的细菌类型以及其群体感应淬灭活性，本研究对土壤中可降解蜂房哈夫尼菌（Hafnia alvei）所产N-酰基高丝氨酸内酯的菌株进行了筛选、分离和鉴定，对群体感应淬灭菌的特性以及其对蜂房哈夫尼菌生物被膜的抑制和清除能力进行了分析，并通过特异性引物扩增判断菌株可能产群体感应淬灭酶类型。共从土壤中分离到4株对蜂房哈夫尼菌所分泌的群体感应信号分子具有较高降解活性的细菌，经鉴定分别为霍氏肠杆菌（Enterobacteri hormaechei）GS31、蜡样芽孢杆菌（Bacillus cereus）GS44、苏云金芽孢杆菌（Bacillus thuringiensis）GS48和沙雷氏菌（Serratia sp.）GS53；其中霍氏肠杆菌GS31的淬灭活性最强，其次为沙雷氏菌GS53、蜡样芽孢杆菌GS44和苏云金芽孢杆菌GS48。霍氏肠杆菌GS31和沙雷氏菌GS53的潜在群体感应淬灭活性物质存在无细胞上清液中（Cell-free supernatant,CFS），而蜡样芽孢杆菌GS44和苏云金芽孢杆菌GS48存在于细胞破碎提取物（Crude cell extract,CCE）中；结合结晶紫染色和光学显微镜分析结果发现，所分离的菌株具有不同程度的抑制和清除蜂房哈夫尼菌生物被膜能力，其中霍氏肠杆菌GS31的抑制率和清除率最高，分别为42.9%和53.0%。同源基因扩增结果表明，霍氏肠杆菌GS31、蜡样芽孢杆菌GS44、沙雷氏菌GS53中可能存在AiiA内酯酶和PvdQ酰化酶基因，苏云金芽孢杆菌GS48中可能存在AiiA内酯酶基因。通过对土壤中可降解N-酰基高丝氨酸内酯菌株的筛选及其群体感应淬灭活性研究，为进一步丰富群体感应淬灭菌资源，以及基于群体感应淬灭菌的水产保鲜剂的开发奠定了基础。

关键词： N-酰基高丝氨酸内酯群体感应群体感应淬灭生物被膜蜂房哈夫尼菌

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RNA-seq analysis reveals the critical role of the novel lnc RNA BIANCR in intramuscular adipogenesis through the ERK1/2 signaling pathway

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Journal of Animal Science and Biotechnology 2023年第3期14卷 917-935页

作者： Xinhao Ma xinran Yang Dianqi Zhang Wenzhen Zhang Xiaoyu Wang Kuncheng Xie Jie He Chugang Mei Linsen Zan College of Animal Science and Technology Northwest A&F UniversityYanglingShaanxi 712100People’s Republic of China Xi’an Dairy Cow Breeding Center Xi’an Agriculture and Rural BureauXi’anShaanxi 712100People’s Republic of China National Beef Cattle Improvement Center Northwest A&F UniversityYanglingShaanxi 712100People’s Republic of China

Background Long non-coding RNAs(lncRNAs)regulate numerous biological processes,including *** on adipogenesis will assist in the treatment of human metabolic diseases and improve meat quality in livestock,such as the content of intramuscular fat(IMF).However,the significance of lncRNAs in intramuscular adipogenesis remains *** research aimed to reveal the lncRNAs transcriptomic profiles in the process of bovine intramuscular adipogenesis and to identify the lncRNAs involved in the adipogenesis of bovine intramuscular *** In this research,a landscape of lncRNAs was identified with RNA-seq in bovine intramuscular adipocytes at four adipogenesis stages(0 d,3 d,6 d,and 9 d after differentiation).A total of 7035 lncRNAs were detected,including 3396 novel *** on the results of differential analysis,co-expression analysis,and functional prediction,we focused on the bovine intramuscular adipogenesis-associated long non-coding RNA(BIANCR),a novel lncRNA that may have an important regulatory *** knockdown of BIANCR inhibited proliferation and promoted apoptosis of intramuscular ***,BIANCR knockdown inhibited intramuscular adipogenesis by regulating the ERK1/2 signaling *** This study obtained the landscape of lncRNAs during adipogenesis in bovine intramuscular *** plays a crucial role in adipogenesis through the ERK1/2 signaling *** results are noteworthy for improving beef meat quality,molecular breeding,and metabolic disease research.

关键词： Adipogenesis BIANCR Bovine Intramuscular adipocyte LncRNAs

RO_(x) Budgets and O_(3) Formation during Summertime at Xianghe Suburban Site in the North China Plain

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Advances in Atmospheric Sciences 2021年第7期38卷 1209-1222页

作者： Min XUE Jianzhong MA Guiqian TANG Shengrui TONG Bo HU xinran ZHANG Xinru LI Yuesi WANG State Key Laboratory of Severe Weather&China Meteorological Administration Key Laboratory of Atmospheric Chemistry Chinese Academy of Meteorological SciencesBeijing 100081China State Key Laboratory of Atmospheric Boundary Layer Physics and Atmospheric Chemistry Institute of Atmospheric PhysicsChinese Academy of SciencesBeijing 100029China State Key Laboratory for Structural Chemistry of Unstable and Stable Species Beijing National Laboratory for Molecular SciencesChinese Academy of Sciences Research/Education Center for Excellence in Molecular SciencesInstitute of ChemistryChinese Academy of SciencesBeijing 100190China Department of Chemistry Capital Normal UniversityBeijing 100081China

Photochemical smog characterized by high concentrations of ozone(O_(3)) is a serious air pollution issue in the North China Plain(NCP)region,especially in summer and *** this study,measurements of O_(3),nitrogen oxides(NO_(x)),volatile organic compounds(VOCs),carbon monoxide(CO),nitrous acid(HONO),and a number of key physical parameters were taken at a suburban site,Xianghe,in the NCP region during the summer of 2018 in order to better understand the photochemical processes leading to O_(3)formation and find an optimal way to control O_(3)***,the radical chemistry and O_(3)photochemical budget based on measurement data from 1−23 July using a chemical box model is *** daytime(0600−1800 LST)average production rate of the primary radicals referred to as RO_(x)(OH+HO2+RO2)is 3.9 ppbv h−*** photolysis is the largest primary RO_(x)source(41%).Reaction of NO2+OH is the largest contributor to radical termination(41%),followed by reactions of RO2+NO2(26%).The average diurnal maximum O_(3)production and loss rates are 32.9 ppbv h−1 and 4.3 ppbv h−1,*** tests without the HONO constraint lead to decreases in daytime average primary RO_(x)production by 55%and O_(3)photochemical production by 42%,highlighting the importance of accurate HONO measurements when quantifying the RO_(x)budget and O_(3)photochemical *** heterogeneous reactions of trace gases and radicals on aerosols,aerosol uptake of HO2 contributes 11%to RO_(x)sink,and the daytime average O_(3)photochemical production decreases by 14%.The O_(3)-NO_(x)-VOCs sensitivity shows that the O_(3)production at Xianghe during the investigation period is mainly controlled by VOCs.

关键词： photochemical smog RO_(x)budgets O_(3)production rate O_(3)chemical regime

Reduction in leaf size at higher altitudes across 39 broad-leaved herbaceous species on the northeastern Qinghai-Tibetan Plateau

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Journal of Plant Ecology 2022年第6期15卷 1227-1240页

作者： xinran Ke Huixing Kang Yanhong Tang Department of Ecology College of Urban and Environmental SciencesPeking UniversityBeijing 100871China

Leaf size varies conspicuously within and among species under different ***,it is unclear how leaf size would change with elevation,whether there is a general elevational pattern,and what determines the altitudinal variation of leaf *** thus aimed to address these questions by focusing on the broad-leaved herbaceous species at high altitudes on the northeastern Qinghai-Tibetan *** measured the leaf size,leaf length,leaf width and leaf mass per area for 39 broad-leaved herbaceous species inhabited in the open areas along two mountain slopes from 3200 to 4400 m at the Lenglongling and the Daban Mountain,the northeastern Qinghai-Tibetan *** analyzed the altitudinal patterns in leaf size in relation to leaf inclination and leaf surface features,and applied a leaf energy balance model to discuss the underlying *** size decreased significantly at higher *** altitudinal reduction of leaf size was mainly attributed to the reduction of leaf length,and differed in different species,and in leaves with different inclination and leaf surface features.A leaf energy balance model with local environmental measurements demonstrates that leaf temperature tracks air temperature more closely in small than in large leaves,and that the leaf-size impact is stronger at higher *** on the observational findings,we propose that the distribution upper-limit for broad-leaved herbaceous species would be at an elevation of about 5400 m on the northeastern Qinghai-Tibetan Plateau.

关键词： elevation leaf area leaf energy balance leaf length leaf mass per area

Adjustable thermal expansion in La(Fe,Si)13-based conductive composites by high-pressure synthesis

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Progress in Natural Science:Materials International 2019年第1期29卷 28-31页

作者： Wei Wang Rongjin Huang Yi Shan Yuqiang Zhao xinran Shan Shibin Guo Chuanjun Huang Laifeng Li School of Materials Science and Engineering Tianjin University State Key Laboratory of Technologies in Space Cryogenic Propellants Technical Institute of Physics and Chemistry Chinese Academy of Sciences University of Chinese Academy of Sciences

Providing a suitable contact interface, where a high conductivity material with a desirable coefficient of thermal expansion(CTE) adjoins the target micro-electric devices, is very crucial to optimize the properties and service life of the relevant instruments. Regrettably, a high conductivity, low thermal expansion and relatively inexpensive material is very rare. Composites, fortunately, can offer a method to design materials with adjustable properties by mixing two or more diverse constituents. In this paper, high conductivity composites with adjustable thermal expansion were successfully prepared by a high-pressure synthesis. The composites are based on combining La(Fe,Si)-based compounds, the materials showing a giant, isotropic negative thermal expansion(NTE) properties, within Cu matrix. The La(Fe,Si)-based compounds were used to adjust the CTE of the composites, while the Cu phase is in charge of tuning the thermal conductivity properties. Thus, by changing the relative amount of the two components, the composites with high conductivities and adjustable CTE were achieved. Furthermore, the thermal expansion and magnetic properties of the composites were investigated by a physical property measurement system. The present results highlight the potential applications of the Cu-based high conductivity composites with room-temperature NTE properties in the thermal contacts to various semiconductor and microelectronic devices.

关键词： La(Fe, Si)13-based compounds Metal-matrix composites Negative thermal expansion

Cellulose synthesis genes CESA6 and CSI1 are important for salt stress tolerance in Arabidopsis

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Journal of Integrative Plant Biology 2016年第7期58卷 623-626页

作者： Shuang-Shuang Zhang Le Sun xinran Dong Sun-Jie Lu Weidong Tian Jian-Xiang Liu State Key Laboratory of Genetic Engineering Collaborative Innovation Center of Genetics and Development School of Life Sciences Fudan University Department of Biostatistics and Computational Biology School of Life Sciences Fudan University

Soil salinity is a widespread abiotic stress constraint threatening agricultural production, as it severely inhibits growth and development of crops. Several salt stress signaling pathways have been discovered in the model plant Arabidopsis thaliana, which include components for sensing the stress, transmitting the signal and regulating the downstream genes （Deinlein et al. 2o14; Julkowska and Testerink 2015）. In nature, plant roots directly encounter heterogeneous soils and therefore their responses to saline environments require dynamic changes in growth, a process in which plant cell walls play important roles （Tenhaken 2o15）. However, the molecular mechanisms underlying cell wall remodeling under stress conditions are still unclear.

关键词： Figure CSI

10,11-Dehydrocurvularin attenuates inflammation by suppressing NLRP3 inflammasome activation

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Chinese Journal of Natural Medicines 2023年第3期21卷 163-171页

作者： ZHAO Qun FENG Mengyuan JIN Shu LIU Xiaobo LI Shengbao GUO Jian CHENG xinran ZHOU Guangbiao YU Xianjun State Key Laboratory of Molecular Oncology National Cancer CenterNational Clinical Research Center for CancerCancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021China Department of Gastroenterology Taihe HospitalHubei University of MedicineShiyan 442000China Laboratory of Inflammation and Molecular Pharmacology School of Basic Medical Sciences&Biomedical Research InstituteSchool of PharmacyHubei Key Laboratory of Wudang Local Chinese Medicine ResearchHubei University of MedicineShiyan 442000China

10,11-Dehydrocurvularin(DCV)is a natural-product macrolide that has been shown to exert anti-inflammatory ***,the underlying mechanism of its anti-inflammatory activity remains poorly *** activation of the NLRP3 inflammasome is involved in diverse inflammation-related diseases,which should be *** results showed that DCV specifically inhibited the activation of the NLRP3 inflammasome in association with reduced IL-1βsecretion and caspase-1 activation,without effect on the NLRC4 and AIM2 ***,DCV disturbed the interaction between NEK7 and NLRP3,resulting in the inhibition of NLRP3 inflammasome *** C=C double bond of DCV was required for the NLRP3 inflammasome inhibition induced by ***,DCV ameliorated inflammation in vivo through inhibiting the NLRP3 *** together,our study reveals a novel mechanism by which DCV suppresses inflammation,which indicates the potential role of DCV in NLRP3 inflammasome-driven inflammatory disorders.

关键词： 10,11-Dehydrocurvularin Inflammation NLRP3 inflammasome NEK7-NLRP3 interaction

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过载和攻击时间约束下的非线性最优制导方法

系统工程与电子技术 2024年第2期46卷 649-657页

作者：王坤段欣然陈征黎军浙江大学航空航天学院浙江杭州310027 浣江实验室浙江诸暨311800

考虑导弹过载受限条件下,对以期望时间为攻击目标的非线性最优制导问题进行了研究。首先,建立了非线性最优制导问题的理论模型,基于庞特里亚金极大值原理和饱和函数方法建立了最优轨迹的最优性条件。其次,根据最优性条件和哈密尔顿轨迹... 详细信息

考虑导弹过载受限条件下,对以期望时间为攻击目标的非线性最优制导问题进行了研究。首先,建立了非线性最优制导问题的理论模型,基于庞特里亚金极大值原理和饱和函数方法建立了最优轨迹的最优性条件。其次,根据最优性条件和哈密尔顿轨迹参数化方法,建立了最优轨迹的参数化微分方程组,使得通过数值积分即可生成从飞行状态到最优制导指令映射关系的数据集。然后,通过前馈神经网络对上述映射关系进行近似,实现了非线性最优制导指令的毫秒量级实时生成。最后,通过数值仿真验证了所提非线性最优制导指令生成方法的有效性。

关键词：过载约束攻击时间控制非线性最优制导哈密尔顿轨迹参数化前馈神经网络

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Defining newly formed and tissue-resident bone marrow-derived macrophages in adult mice based on lysozyme expression

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Cellular & Molecular Immunology 2022年第12期19卷 1333-1346页

作者： Tong Lei Jiayu Zhang Qian Zhang xinran Ma Yanan Xu Yang Zhao Lianfeng Zhang Zhongbing Lu Yong Zhao State Key Laboratory of Membrane Biology Institute of ZoologyChinese Academy of SciencesBeijingChina College of Life Science University of Chinese Academy of SciencesBeijingChina Cunji Medical School University of Chinese Academy of SciencesBeijingChina Key Laboratory of Human Diseases Comparative Medicine Ministry of HealthInstitute of Laboratory Animal ScienceChinese Academy of Medical SciencesPeking Union Medical CollegeBeijingChina Beijing Institute for Stem Cell and Regenerative Medicine BeijingChina

Tissue-resident macrophages are derived from different precursor cells and display different *** of the tissue-resident macrophages of inflamed or damaged tissues in adults can be achieved by bone marrow-derived monocytes/*** lysozyme(Lysm)-GFP-reporter mice,we found that alveolar macrophages(AMs),Kupffer cells,red pulp macrophages(RpMacs),and kidney-resident macrophages were Lysm-GFP^(−),whereas all monocytes in the fetal liver,adult bone marrow,and blood were Lysm-GFP^(+).Donor-derived Lysm-GFP^(+)resident macrophages gradually became Lysm-GFP−in recipients and developed gene expression profiles characteristic of tissue-resident ***,Lysm may be used to distinguish newly formed and long-term surviving tissue-resident macrophages that were derived from bone marrow precursor cells in adult mice under pathological ***,we found that Irf4 might be essential for resident macrophage differentiation in all tissues,while cytokine and receptor pathways,mTOR signaling pathways,and fatty acid metabolic processes predominantly regulated the differentiation of RpMacs,Kupffer cells,and kidney macrophages,*** in ST2,mechanistic target of rapamycin(mTOR)and fatty acid-binding protein 5(FABP5)differentially impaired the differentiation of tissue-resident macrophages from bone marrow-derived monocytes/macrophages in the lungs,liver,and *** results indicate that a combination of shared and unique signaling pathways coordinately shape tissue-resident macrophage differentiation in various tissues.

关键词： Tissue-resident macrophages Kupffer cells Lysozyme Inflammation Metabolism Cytokine