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Development and characterization of monoclonal antibodies against human aryl hydrocarbon receptor

Journal of Environmental Sciences 2016年第1期28卷 165-174页

作者： Wenjing Tian xinhui Pei Heidi Qunhui Xie Sherry Li xu Jijing Tian Qin Hu Haiming xu Yangsheng Chen Hualing Fu Zhengyu Cao Bin Zhao Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing I00085 China China Pharmaceutical University Nanjing 211198 China

Aryl hydrocarbon receptor（Ah R）, a ligand-dependent nuclear receptor, is involved in a diverse spectrum of biological and toxicological effects. Due to the lack of three dimensional（3D）crystal or nuclear magnetic resonance structure, the mechanisms of these complex effects of AhR remain to be unclear. Also, commercial monoclonal antibodies（mA bs） against human AhR protein（h Ah R）, as alternative immunological tools, are very limited. Thus, in order to provide more tools for further studies on h Ah R, we prepared two m Abs（1D6 and 4A6） against h Ah R. The two newly generated m Abs specifically bound to amino acids 484–508（located in transcription activation domain） and amino acids 201–215（located in Per-ARNT-Sim domain）of h Ah R, respectively. These epitopes were new as compared with those of commercial m *** m Abs were also characterized by enzyme-linked immunosorbent assay, western blot,immunoprecipitation and indirect immunofluorescence assay in different cell lines. The results showed that the two m Abs could recognize the linearized AhR s in six different human cell lines and a rat hepatoma cell line, as well as the h Ah R with native conformations. We concluded that the newly generated m Abs could be employed in AhR-based bioassays for analysis of environmental contaminants, and held great potential for further revealing the spatial structure of AhR and its biological functions in future studies.

关键词： Aryl hydrocarbon receptor Monoclonal antibody Western blot Immunoprecipitation Indirect immunofluorescence assay

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香芹酚对恶臭假单胞菌的抑制作用

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肉类研究 2024年第6期38卷 71-75页

作者：李佳玟吴轩潘雅洁肖龙泉刘达玉王新惠徐纲周闯成都大学食品与生物工程学院四川成都610106 宜宾市娥天歌食品有限公司四川宜宾644100

以恶臭假单胞菌(Pseudomonas putida)为对象,通过测定恶臭假单胞菌生长曲线、群集性、泳动性、核酸泄漏情况及产胞外蛋白酶和生物被膜能力等指标,考察不同体积分数香芹酚对恶臭假单胞菌的抑菌效果。结果表明:当香芹酚体积分数≥0.015%时... 详细信息

以恶臭假单胞菌(Pseudomonas putida)为对象,通过测定恶臭假单胞菌生长曲线、群集性、泳动性、核酸泄漏情况及产胞外蛋白酶和生物被膜能力等指标,考察不同体积分数香芹酚对恶臭假单胞菌的抑菌效果。结果表明:当香芹酚体积分数≥0.015%时,对恶臭假单胞菌的生长、运动能力、产胞外蛋白酶和生物被膜能力具有极显著抑制作用(P<0.01);当香芹酚体积分数为0.025%时,恶臭假单胞菌所产生物被膜含量比空白组极显著减少86.35%(P<0.01)。香芹酚可通过抑制生长、产生物被膜能力及运动能力破环细胞膜通透性,导致大量细胞内容物泄漏,从而对恶臭假单胞菌产生抑菌作用。

关键词：恶臭假单胞菌香芹酚鲜肉保鲜泳动性群集性

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中枢神经系统曲霉病1例

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中国感染与化疗杂志 2011年第5期11卷 386-388页

作者：黄欢张柯基何双军徐欣晖周巍陈怡吕利雄朱长清上海交通大学医学院附属仁济医院急诊与危重病学科 200127

过去的30年中,侵袭性曲霉病的病例报道逐渐增多。尽管中枢神经系统（CNS）曲霉感染并不常见,但临床进展迅速,预后极差。由于组织活检病理检查较难实施,因此诊断常依靠临床判断。本文通过我院诊断的1例CNS曲霉病,结合文献报道对该病进行... 详细信息

过去的30年中,侵袭性曲霉病的病例报道逐渐增多。尽管中枢神经系统（CNS）曲霉感染并不常见,但临床进展迅速,预后极差。由于组织活检病理检查较难实施,因此诊断常依靠临床判断。本文通过我院诊断的1例CNS曲霉病,结合文献报道对该病进行回顾复习。

关键词：中枢神经系统曲霉病抗真菌药物

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Influence of Climate on Soil Organic Carbon in Chinese Paddy Soils

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Chinese Geographical Science 2017年第3期27卷 351-361页

作者： WANG Dandan YAN Yechao LI xinhui SHI xuezheng ZHANG Zhongqi David C WEINDORF WANG Hongjie xu Shengxiang School of Geography and Remote Sensing Nanjing University of Information Science & Technology Nanjing 210044 China State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil Science Chinese Academy of Sciences Nanjing 210008China Jiangsu Normal University Xuzhou 221116 China Department of Plant and Soil Sciences Texas Tech University Lubbock 79409 USA

Soil organic carbon(SOC) is a major component of the global carbon cycle and has a potentially large impact on the greenhouse effect. Paddy soils are important agricultural soils worldwide, especially in Asia. Thus, a better understanding of the relationship between SOC of paddy soils and climate variables is crucial to a robust understanding of the potential effect of climate change on the global carbon cycle. A soil profile data set(n = 1490) from the Second National Soil Survey of China conducted from 1979 to 1994 was used to explore the relationships of SOC density with mean annual temperature(MAT) and mean annual precipitation(MAP) in six soil regions and eight paddy soil subgroups. Results showed that SOC density of paddy soils was negatively correlated with MAT and positively correlated with MAP(P < 0.01). The relationships of SOC density with MAT and MAP were weak and varied among the six soil regions and eight paddy soil subgroups. A preliminary assessment of the response of SOC in Chinese paddy soils to climate indicated that climate could lead to a 13% SOC loss from paddy soils. Compared to other soil regions, paddy soils in Northern China will potentially more sensitive to climate change over the next several decades. Paddy soils in Middle and Lower Yangtze River Basin could be a potential carbon sink. Reducing the climate impact on paddy soil SOC will mitigate the positive feedback loop between SOC release and global climate change.

关键词： soil organic carbon paddy soils mean annual temperature mean annual precipitation climate change

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Synergetic Alkoxy Side-Chain and Chlorine-Contained End Group Strategy toward High Performance Ultra-Narrow Bandgap Small Molecule Acceptors

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Chinese Journal of Chemistry 2023年第20期41卷 2664-2670页

作者： Qingya Wei Songting Liang Beibei Qiu Wei Liu Xiang xu xinhui Lu Yuang Fu Jun Yuan Yingping Zou State Key Laboratory of Powder Metallurgy College of Chemistry and Chemical EngineeringCentral South UniversityChangsha Hunan 410083China Key Laboratory of Solid State Optoelectronic Devicesof Zhejiang Province College of Physics and Electronic Information EngineeringZhejiang Normal UniversityJinhuaZhejiang 321004China Departmentof Physics The Chinese University of Hong KongNew TerritoriesHong Kong 999077China School of Materials Science and Engineering Zhengzhou UniversityZhengzhouHenan 450001China

Ultra-narrow bandgap(ultra-NBG)small molecule acceptors(SMAs)show great potential in organic solar cells(OSCs)due to the extended near-infrared(NIR)*** this work,a synergetic alkoxy side-chain and chlorine-contained end group strategy is employed to achieve A-DA'D-A type ultra-NBG SMAs by introducing alkoxy chains with oxygen atom at the second position into the thiopheneβposition as well as replacing the F atoms with Cl atoms in the end *** a result,the heptacyclic BZO-4F shows a redshifted absorption onset(960 nm)compared with Y11(932 nm)without oxygen atoms in the side ***,the fluorinated end groups are substituted with the chlorinated ones to synthesize *** absorption onset of BZO-4Cl is further redshifted to 990 nm,corresponding to an optical ultra-NBG of 1.25 *** blending with the polymer donor PBDB-T,the binary devices based on PBDB-T:BZO-4F and PBDB-T:BZO-4Cl deliver power conversion efficiencies(PCEs)over 12%.Furthermore,ternary devices with the addition of BZ4F-O-1 into PBDB-T:BZO-4Cl system achieve the optimal PCE of 15.51%.This work proposes a synergetic alkoxy side-chain and chlorine-contained end group strategy to achieve A-DA'D-A type ultra-NBG SMAs,which is important for future molecular design.

关键词： Organic solar cells A-DA'D-A type small molecule acceptors Ultra-narrow bandgap Alkoxy side chains Ternary strategy π-πstacking

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以临床技能培养为导向的中医护理学教学改革探索

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中国中医药现代远程教育 2018年第7期16卷 34-35页

作者：邹颖张轶卢洪梅邓少东许新晖卢阳佳成晓燕迟宏罡广东医科大学第二临床医学院中医学教研室广东东莞523808

中医护理学是护理学专业的一门重要课程,本教研室对该课程的教学大纲、教学内容、教学方法和考核方式等进行了一系列的改革和探索,积累了丰富的教学经验,也培养了护理专业学生的学习兴趣,促使他们把中医学的知识技能运用到临床护理工作... 详细信息

中医护理学是护理学专业的一门重要课程,本教研室对该课程的教学大纲、教学内容、教学方法和考核方式等进行了一系列的改革和探索,积累了丰富的教学经验,也培养了护理专业学生的学习兴趣,促使他们把中医学的知识技能运用到临床护理工作中,不断提升实践应用能力,教学效果显著。

关键词：中医护理学教学改革临床技能

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Genomic selection of orange-spotted grouper(Epinephelus coioides)based on multiplex PCR enrichment capture sequencing

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Aquaculture and Fisheries 2023年第6期8卷 681-688页

作者： Xinxin Shan xinhui Zhang Zhiqiang Ruan Jieming Chen Qiong Shi Junmin xu Xinxin You Shenzhen Key Lab of Marine Genomics Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic AnimalsBGI Academy of Marine SciencesBGI MarineBGIShenzhen518083China Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai) State Key Laboratory of BiocontrolSchool of Marine SciencesSun Yat-Sen UniversityGuangzhou510275China College of Life Sciences University of Chinese Academy of SciencesBeijing100049China

Orange spotted grouper(Epinephelus coioides)is an important mariculture fish,and genomic breeding of this grouper species has been hindered due to lack of efficient genotyping ***,we developed a single nucleotide polymorphism(SNP)genotyping technology based on multiplex PCR enrichment capture sequencing,which mainly aims at target area for high-throughput sequencing,and 741 SNPs were designed for genomic selection(GS)of growth and ammonia tolerance traits at the same *** multiplex PCR enrichment capture sequencing assay showed that the genotyping efficiency was more than 99%in the orange-spotted grouper and the predictive accuracy of body weight and ammonia tolerance traits was 82%and 96%,*** importantly,the average identity of the sequences with these SNPs aligned to the genomes of giant grouper(***)and brown-marbled grouper(***)were both over 96%.Test data showed that the SNP genotyping efficiency was more than 94%in both giant grouper and brown-marbled *** summary,these results indicated that the development of SNP loci and genotyping approach based on the multiple PCR enrichment capture sequencing are suitable for GS of growth and ammonia tolerance traits in various grouper species,and it would provide technical support for practical grouper breeding.

关键词： Grouper SNP genotyping Approach Multiple PCR enrichment Capture sequencing Genomic selection

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基于ferritin纳米载体的抑制素α亚基的多克隆抗体的制备

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基因组学与应用生物学 2024年第5期43卷 877-889页

作者：韩璐宋新辉徐佳寅张家宁覃朝斌羊玉凤刘庆友李志鹏广西大学动物科学技术学院亚热带农业生物资源保护与利用国家重点实验室南宁530004 佛山科学技术学院生命科学与工程学院动物分子设计与精准育种广东省重点实验室佛山528225

为了制备高效价和多动物适用性的抑制素α(inhibinα,INH-α)多克隆抗体,通过生物信息学分析INH-α物种同源保守性,并以pET-32α为骨架,构建INH-α和INH-α-Ferritin表达载体,原核表达纯化后的重组蛋白用于免疫日本大耳兔,采用间接ELIS... 详细信息

为了制备高效价和多动物适用性的抑制素α(inhibinα,INH-α)多克隆抗体,通过生物信息学分析INH-α物种同源保守性,并以pET-32α为骨架,构建INH-α和INH-α-Ferritin表达载体,原核表达纯化后的重组蛋白用于免疫日本大耳兔,采用间接ELISA方法测定兔血清中抗体效价。结果表明,本研究成功构建了INH-α和INH-α-Ferritin原核表达载体,在0.4 mmol/L IPTG、37℃诱导6 h的条件下,分别获得了35 kDa和50 kDa包涵体形式的重组蛋白。通过免疫获得了高效价的兔抗多克隆抗体,INH-α-Ferritin抗血清效价达到1∶19531250。生物信息学分析结果显示INH-α在哺乳动物间的同源性高、保守性强。本研究成功构建INH-α和INH-α-Ferritin原核表达载体,并通过INH-α-Ferritin显著提高多克隆抗体的效价,为提高哺乳动物家畜繁殖力的研究奠定基础。

关键词：抑制素α 生物信息学原核表达多克隆抗体

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Fast screening compositions of PM2.5 by ATR-FTIR： Comparison with results from IC and OC/EC analyzers

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Journal of Environmental Sciences 2018年第9期30卷 76-88页

作者： xu Yu Wei Song Qingqing Yu Sheng Li Ming Zhu Yanli Zhang Wei Deng Weiqiang Yang Zhonghui Huang xinhui Bi Xinming Wang State Key Laboratory of Organic Geochemistry and Guangdong Key Laboratory of Environmental Protection and Resources Utilization Guangzhou Institute of Geochemistry Chinese Academy of Sciences Guangzhou 510640 China Center for Excellence in Regional Atmospheric Environment Institute of Urban Environment Chinese Academy of SciencesXiamen 361021 China Uniuersity of Chinese Academy of Sciences Beijing 100049 China Department of Chemistry Hong Kong University of Science and Technology Kowloon Hong Kong China

Chemical speciation of fine particles or PM2.5 collected on filters is still a cosily and time- consuming task. In this study, filter-based PM2.5 samples were collected during November-December 2013 at four sites in Guangzhou, and the major components were fast screened （~ 7 rain per filter sample） by Attenuated Total Reflectance （ATR）-Fourier Transform Infrared Spectroscopic （FTIR） in comparison with that measured by Organic carbon/Element carbon （OC/ EC） analyzer and Ion Chromatography （IC）. The concentrations of nitrate, ammonium, sulfate, primary organic carbon （POC） and secondary organic carbon （SOC） measured by OC/EC and IC analyzers were better correlated with their infrared absorption peak heights at 1320 cm 1 for nitrate, 1435, 3045 and 3215 cm^-1 for ammonium, 615 cm^-1 for sulfate, 690, 760 and 890 cm^-1 for POC and 1640 and 1660 cm^-1 for SOC respectively, during polluted days （PM2.5 〉 75 μg/m^3） than during clean days （PM2.5〈 75 μg/m^3）. With the evolution of a haze episode during our field campaign, the concentrations of the major PM2.5 components displayed consistent variations with their infrared absorption peak heights, suggesting ATR-FTIR could be a fast and useful technique to characterize filter-based PM2.5 compositions particularly during pollution events although cautions should be taken when PM2.5 levels are low. Notably, elevated PM2.5 mass concentrations occurred with enhanced ratios of [NO^-3][SO^2-4] and [NH^＋4]/[SO^2-4], implying that nitrogenous components play vital roles in the PM2.5 pollution events in the study region.

关键词： ATR-FTIR Fine particles (PM2.5) Haze episode Sulfate Nitrate Ammonium Organic carbon

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Alkyl side chain engineering enables high performance as-cast organic solar cells of over 17%efficiency

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Fundamental Research 2023年第4期3卷 611-617页

作者： Xiang xu Yazhou Qi Xiaoyan Luo Xinxin Xia xinhui Lu Jun Yuan Yonghua Zhou Yingping Zou State Key Laboratory of Powder Metallurgy College of Chemistry and Chemical EngineeringCentral South UniversityChangsha 410083China Department of Physics The Chinese University of Hong KongNew TerritoriesHong Kong 999077China

Achieving high-performance as-cast OSCs is crucial for industrialization in the future,owing to the advantages of better stability,environmental-friendly,and decreasing production *** this regard,we synthesized an ADA′D-A type acceptor,Y6-eC6-BO,by shortening the straight alkyl side-chains on the thiophene position from C_(11) to C_(6) as well as lengthening the branched alkyl side-chains on the pyrrole position of Y6 to achieve a stronger crystallization and better miscibility than *** a result,the corresponding chloroform-processed as-cast PM6:Y6-eC6-BO OSC showed a high PCE of 17.33%,which was one of the highest efficiencies of as-cast *** the as-cast PM6:Y6-eC6-BO OSCs processed from o-xylene displayed a PCE of 16.38%,as far as we know,this is among the highest efficiencies of non-halogenated-solvent processed as-cast *** results demonstrated tailoring the alkyl side-chain of NFAs is a feasible and simple approach to achieve high performance as-cast OSCs and provides guideline in molecular design in the future.

关键词： Organic solar cells Alkyl side chains As-cast Green solvent Stability

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