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Effect of Echinacea Polysaccharide on Secretion of IL-1αmRNA by IEC-6 Injured by LPS

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Agricultural Biotechnology 2016年第6期5卷 35-36,43页

作者： Yanying ZHANG Qiumei SHI Qinghui JIA Guisheng GAO Guangping GAO xinhua shao Hebei Normal University of Science&Technology Hebei Province Key Laboratory of Preventive Veterinary MedicineChangli 066600China xinhua Keji Veterinary Medicine Co. Ltd.of Hebei ProvinceLuancheng 051430China

[ Objective ] This study was conducted to investigate Echinacea polysaccharide （EPS） on expression of tumor necrosis factor （TNF） under injury of intestinal epithelial cells （IEC-6） by lipopolysaccharide （LPS）, so as to discuss the action mechanism of EPS to injured cells. [Method] Total DNA was extracted with TRIzon reagent, TNF-α mRNA was amplified, and the amplification products were subjected to agarose gel electrophoresis and imaging. [Result] 50 μg/ml EPS could partially inhibited the production of IL-α mRNA by IEC-6 under the stimulation of LPS, while the inhibition of 2（10 and 500 μg/ml EPS on the level of IL-1α mRNA gradually increased with the concentration increasing; and IEC-6 cells pretreated with 50,100,200 and 500 μg/ml EPS for 24 h and then stimulated by 10 μml IPS for 1 and 4 h were analyzed by RT-PCR method, and it was found that the expression of IL-α mRNA induced by LPS could be effectively inhibited by EPS, and the inhibition rate at 4 h was higher than that at 1 h. [ Conclusion] EPS could play its role of protecting intestinal mucosa by inhibiting the secretion of IL-1α mRNA by cells under the stimulation of LPS, and such inhibition effects of EPS had concentration dependency and time dependency.

关键词： Echinacea pelysaccharide Lipopelysaccharide Intestinal epithelial cell Inhibition Protection IL-1αmRNA

In Vitro Antibacterial Effect of Traditional Chinese Medicine on Main Pathogenic Bacteria of Recessive Mastitis in Dairy Cows

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Animal Husbandry and Feed Science 2017年第1期9卷 34-36页

作者： Gao Guisheng Hu Tiefeng An Jiwei Gao Guangping Shi Qiumei shao xinhua Zhang Yanying Hebei Provincial Key Laboratory of Veterinary Preventive Medicine Hebei Normal University of Science and TechnologyChangli 066600China Animaland Veterinary Bureau of Laoting County Tangshan 063600China Hebei xinhua Veterinary Drug Co. Ltd.Luancheng 051430China

The inhibition zone diameter, minimal inhibitory concentration （MIC） and minimum bactericidal concentration （MBC） of 20 kinds of traditional Chi- nese medicine on Staphylococcus aureus and Streptococcus agalactiae isolated from recessive mastitis were studied using plate test and tube test. The results showed that S. aureus and S. agalactiae were sensitive to all these 20 kinds of traditional Chinese medicine, and were more sensitive to seven kinds of traditional Chinese medicine, including Taraxtwum mongolicum, Coptis chinensis, Forsythia suspense, Andrographis paniculata, Carthamus tinctorius, Scutellaria baicalensis and Den- dranthema indicum.

关键词： Traditional Chinese medicine Streptococcus agalactiae Inhibition zone diameter MIC MBC

Diagnosis and Control of Rabbit Hemorrhagic Disease(RHD)

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Agricultural Biotechnology 2016年第1期5卷 44-46,49页

作者： Fang XIANG Rui GUO Hongxue ZHANG Fujin LI Changhao LIU Qiumei SHI xinhua shao Changli Animal Epidemic Disease Prevention and Control Center Changli 066600China Qilu Animal Health Products Co. Ltd.Ji'nan 2501002China Key Laboratory of Preventive Veterinary Medicine of Hebei Province/Hebei Normal University of Science and Technology Changli 066600China Hebei xinhua Keji Veterinary Drug Co. Ltd.Luancheng 051430China

In this study, chronic-type rabbit hemorrhagic disease occurred in rabbit warrens of Shandong, Liaoning, Beijing and Hebei, which was mainly charac-terized by deaths of young and adult rabbits, longer durationand nerve symptoms, was surveyed by epidemiological investigation, bacterial isolation and incubation, artificial infection test and histopathologieal method to analyze the causes of chronic-type rabbit hemorrhagic disease and put forward the corresponding control measures.

关键词： Rabbit Chronic-type rabbit hemorrhagic disease Diagnosis and treatment

PCR Detection of Streptococcus agalactiae in Dairy Cows Suffering from Mastitis

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Agricultural Biotechnology 2013年第5期2卷 22-24页

作者： Yanying ZHANG Guisheng GAO Zhengben LI Guangping GAO Qiumei SHI xinhua shao Yinju LIANG Huiran LIU Key Laboratory of Preventive Veterinary Medicine of Hebei Province College of Animal Science and TechnologyHebei Normal University of Science and Technology Jinfuzhi Dairy Farm of Qian'an City Hebei xinhua Animal Drug Co. Ltd.

[ Objective ] This study aimed to identify Streptococcus agalactiae and lay the foundation for the prevention and control of mastiffs in dairy cows. [ Method] Ten strains were isolated from milk samples produced by diseased dairy cows suffering from mastitis for morphologic observation, culture characteristic investigation, biochemical identification and Lancefield grouping. The isolated strains were identified at the molecular level by nested-PCR. [ Result] Among the ten isolates, six strains were 13-hemolytic and Gram-positive on blood agar, belonging to Lancefield group B, which were identified as Streptococcus agalactiae by biochemical identification and nested-PCR. After overnight incubation, the coincidence rate between results of nested-PCR detection and biochemical identification reached 100%. [ Conclusion ] Bacterial incubation, rapid DNA extraction and specific PCR can provide basis for early epidemiological survey of Streptococcus agalactiae infection in cattle.

关键词： Mastitis Streptococcus agalactiae PCR 16S rRNA

Particle Sizes and Antibacterial Activity of Radix Astragalus and Radix Isatidis Ultrafine Powders

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Agricultural Biotechnology 2016年第4期5卷 42-45,50页

作者： Qiumei SHI xinhua shao Xiumin WANG Xia MENG Xiaoqiao HOU Baoxin YANG Leiyu GUO Jinglong GAO Key Laboratory of Preventive Veterinary Medicine of Hebei Province/Hebei Normal University of Science and Technology Changli 066600China Hebei xinhua Keji Pharmaceuticals Corporation Luancheng 051430China Beijing Shengtaier Biological Technological Co. Ltd.Beijing 102206China Yangzhou University Yangzhou 225009China Zhonghong Sanrong Group Co. Ltd.Chengde 067499China

Using 9200 laser particle size analyzer and KYKY-2800 scanning electron microscope, particle sizes and cellular morphology of Radix Astragalus and Radix Isatidis ultrafine powders were observed. According to the results, the particle size of 89. 1 % of Radix Astragalus ultrafine powders ranged from 1.729 [xm to 44.938 ｜xm, Z ）50 =4.368 ｜xm; the particle size of 93.411% of Radix Isatidis ultrafine powders ranged from 1.510 [xm to 44.938 ｜xm, Z ）50 = 8 .7 2 6 [xm. Radix As-tragalus and Radix Isatidis ultrafine powders were pulverized completely without intact cellular morphology. The antibacterial activity of Radix Astragalus and Radix Isatidis ultrafine powders against chicken-derived E. coli （078） was investigated. The results indicated that Radix Astragalus and Radix Isatidis ultrafine powders exhibited higher antibacterial activity against chicken-derived E. coli （078 ） compared with the corresponding coarse powders. This study laid a solid foundation for the development and application of Chinese medicine ultrafine powder preparations.

关键词： Radix Astragalus Radix Isatidis Ultrafine powder Antibacterial test

Histopathological Observation of Goose Escherichia coli Disease

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Agricultural Biotechnology 2013年第5期2卷 28-31页

作者： Ping SHEN Guangping GAO Guisheng GAO Yanying ZHANG Qiumei SHI xinhua shao Lining HE Wenwen SU Key Laboratory of Preventive Veterinary Medicine of Hebei Province Hebei Normal University of Science and Technology Hebei xinhua Animal Drug Co. Ltd. Shijiazhuang Institute of Veterinary Drug Control

[ Objective] This study aimed to provide reliable basis for in-depth understanding of histopathological changes in geese suffering from Escherichia coli disease and accurate diagnosis of goose E. coli disease. [ Method] By using paraffin embedding, section slicing and HE staining, histopathological changes in the heart, liver, spleen, lung, kidney and small intestine tissues of three diseased geese were observed. [ Result] The liver generated protein fiber membranes, showing liver cell degeneration, increasing number of red blood cells in sinusoids and liver congestion; the kidney had relatively serious lesions, including renal tubular epithelial cell degeneration, necrosis and shedding from the basement membrane and hyaline degeneration of glomerulars. The lung showed epithelial cell degeneration and necrosis and was filled with a large number of red blood cells and shedded epithelial cells, showing typical red hepatization; a large number of red blood ceils were aggregated in pulmonary blood vessels, resulting in lung congestion. Epithelial ceils of various digestive tubes all showed varying degrees of minor shed- ding and local infiltration of inflammatory ceils, thereby leading to death of geese due to substance metabolism, electrolyte and acid-base balance disorders and metabolic acidosis. [ Conclusion] Based on epidemiology survey, clinical symptom observation, pathological necropsy and laboratory diagnosis, the diseased geese were confirmed to be suffering from E. coli disease.

关键词： Goose Escherichia coli Histopathology Observation

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非编码RNA调控骨关节炎疾病的研究进展

中华地方病学杂志 2020年第5期39卷 386-390页

作者：邵新华邹宁哈尔滨医科大学中国疾病预防控制中心地方病控制中心中心实验室 150081

非编码RNA(non-coding RNA,ncRNA)在基因表达调控过程中起着重要作用,其在疾病发生、发展中的作用已成为当下研究热点之一.骨关节炎(osteoarthritis,OA)是一种与年龄密切相关的活动关节慢性退行性病变,主要由年龄、外伤等因素引起软骨... 详细信息

非编码RNA(non-coding RNA,ncRNA)在基因表达调控过程中起着重要作用,其在疾病发生、发展中的作用已成为当下研究热点之一.骨关节炎(osteoarthritis,OA)是一种与年龄密切相关的活动关节慢性退行性病变,主要由年龄、外伤等因素引起软骨细胞的功能逐渐降低,合成及代谢能力逐渐下降,不能有效地合成软骨基质来维持软骨量和软骨结构,进而导致了OA的发生.近年来,随着OA发病机制的研究在分子生物学领域的不断进展,关于OA与ncRNA关系的研究也逐渐深入.本文对ncRNA在OA发病过程中起调控作用的相关研究进行综述,为OA的防治及临床治疗提供参考依据.

关键词：非编码RNA 骨关节炎调控机制

Phylogenetic Analysis of Virulence Factor Genes in Salmonella from Chicken

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Agricultural Biotechnology 2016年第3期5卷 54-56页

作者： Qiumei SHI Yanying ZHANG Xiumin WANG Baoxin YANG Zhiqiang ZHANG Tonglei WU Xiaoqiao HOU xinhua shao Guoqiang ZHU Key Laboratory of Preventive Veterinary Medicine of Hebei Province/Hebei Normal University of Science and Technology Changli 066600China Beijing Centre Biology Co. Ltd.Beijing 102600China Hebei xinhua KeJi Pharmaceutical(Group)Corporation Luancheng 051430China Yangzhou University Yangzhou 25009China

Salmonella is a common genus of seriously harmful food-borne zoonotic bacteria. Humans and animals may be infected with Salmonella through ingestion of SalmoneUa-contaminated eggs and poultry meat. Therefore, in order to reduce the incidence of Salmonella infections, it is crucial to explore the pathogenic mech- anism of Salmonella. invA and invE are major virulence factor genes that encode invasion proteins of Salmonella. In order to explore the pathogenic mechanism of Salmonella, phylogenetic analysis of major virulence factor genes in 33 Salmonella strains isolated from chicken was analyzed. According to the results, ivnA gene was successfully amplified from 33 Salmonella strains; ivnE gene was successfully amplified from 32 Salmonella strains, ivnA nucleotide sequences shared 72.9% - 97.6% homology among 12 sequenced Salmonella strains and shared 78.9% - 97.2% homology with those in GenBank ; ivnE nucleotide sequences shared over 95.3% homology among 23 sequenced Salmonella strains and shared 89.6% -98.6% homology with those in GenBank, which exhibited no genetic relationship to other organisms. This study provided the basis for rapid molecular detection, epidemiological research and molecular pathogenesis analysis of Salmonella.

关键词： Salmonella Virulence factor gene PCR

PCR Detection of Pilus-associated Genes and Serotype Identification of Pathogenic Escherichia coli Isolated from Chickens in the Jidong Area

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Agricultural Biotechnology 2016年第2期5卷 43-45页

作者： Qiumei SHI Yanying ZHANG Xiumin WANG Baoxin YANG Zhiqiang ZHANG Xiaojiao Hou xinhua shao Xia MENG Yang YANG Guoqiang ZHU Key Laboratory of Preventive Veterinary Medicine of Hebei Province/Hebei Normal University of Science and Technology Changli 066600China Beijing Centre Biology Co. Ltd.Beijing 102600China Hebei xinhua Keji Pharmaceutical(Group)Corporation Luancheng 051430China Yangzhou University Yangzhou 225009China

[ Objective] This study aimed to explore the presence of type I pill （fimC gene） and P pill （papC gene） and identify the serotype of pathogenic E. coli isolated from chickens in the Jidong Area. [ Method] Type I pill （fimC gene） and P pill （papC gene） were detected by PCR. The serotype was identified by con- ventional agglutination test. [ Result ] The results showed that 100% of chicken-derived E. coil strains expressed type I pill （fimC gene） ; 39.1% （9/23） of chick- en-derived E. coli strains expressed P pill （papC gene）. In addition, 23 isolates of chicken-derived E. coil were assigned to 14 O serotypes, including O78, O93, O 45, O101, O38, O88, O24, O1, O163, O53, O15, O87, O34 and O29, among which O78 was the dominant serotype that accounted for 42.8% （6/14） of the total strain number. [ Conclusion] Chicken-derived E. coli strains in the Jidong Area belonged to 14 serotypes, and 078 was the dominant serotype; 83.3% of 078 serotvDe E. coli strains expressed both tvDe I Dill and P Dill.

关键词： Chicken Escherichia coli Serotype Pili