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Systematic review and meta-analysis on phosphodiesterase 5 inhibitors and α-adrenoceptor antagonists used alone or combined for treatment of LUTS due to BPH

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Asian Journal of Andrology 2015年第6期17卷 1022-1032,I0012页

作者： xing-huan wang Xiao wang Ming-Jun Shi Sheng Li Tao Liu Xin-Hua Zhang Department of Urology Zhongnan Hospital of Wuhan University Wuhan 430071 China.

The aim of this systematic review is to determine the comparative effectiveness and safety of phosphodiesterase 5 inhibitors （PDE5-1s） and （x-blockers used alone or combined for the treatment of lower urinary tract symptoms （LUTS） due to benign prostatic hyperplasia （BPH）. An electronic search of PubMed, Cochrane Library and Embase up to January 2014 was performed to identify randomized controlled trials comparing the efficacy and safety of PDE5-Is and （x-blockers for treatment of lower urinary tract symptoms due to benign prostatic hyperplasia, which assessed IPSS score, maximum flow rate, postvoided residual urine, quality of life and Erectile Function （IIEF） score as outcomes. Data were analyzed by fixed or random effect models using Cochrane Collaboration review manager software. A total of 12 studies were included, Our novel data demonstrated that there was a trend that （x-blockers were more efficacious than PDE5-Is on decreasing IPSS score and increasing maximum flow rate. （x-blockers were significantly more effective than PDE5-Is on reduction of postvoided residual urine with a mean difference of 3.67 （95% CI 1.56 to 5.77, P = 0.0006） and PDE5-Is showed greater effect than （x-blockers on increasing IIEF score with a mean difference of 9.82 （95% CI 3.80 to 15.85, P = 0.001）. In conclusion, our novel data demonstrated that PDE5-Is plus ABs ranked the highest on the improvement of LUTS/BPH. PDE5-Is monotherapy was also effective in this kind of disorder except less reduction of PVR than ABs, In addition, both combined- or mono-therapy were safe.

关键词： benign prostatic hyperplasia lower urinary tract symptoms meta-analysis phosphodiesterase 5 inhibitors -adrenoceptorantagonists

A novel rat model of seminal vesiculitis

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Asian Journal of Andrology 2019年第4期21卷 360-364页

作者： Peng Zhang Xiao-Long wang Zhong-Hua Yang Xin-Jun Su xing-huan wang Department of Urology Zhongnan Hospital Wuhan University Wuhan 430071 China Department of Urology Research Lab/LIFE-Zentrum University of Munich (LMU) Munchen 81377 Germany

We aimed to establish a novel rat model of seminal vesiculitis that would provide an effective approach to investigate the pathogenesis of this disease in the future. Eight male rats received the same operation, during which the root of one of the two seminal vesicles was partly ligatured with sutures and the other vesicle was left in tact. The samples of seminal vesicles were harvested on the 8th day followi ng the operation. Hematoxylin and eosi n and Mass on's trichrome stains were used to observe the histopathology and the presenee of fibrous tissue in seminal vesicles, respectively. Immunoblotting and immunohistochemistry were applied to determine the tumor n ecrosis factor-alpha and cyclooxygenase-2 levels in semi nal vesicle tissues. Real-time fluoresce nee qua ntitative polymerase chain reaction was performed to measure the gene expression levels of proinflammatory cytokines. H2O2 levels in the seminal plasma from the seminal vesicle were also measured. Hematoxylin and eosin staining suggested that there was inflammatory cell in filtration into the semi nal vesicles treated by partial root ligati on. The tumor n ecrosis factor-alpha and cyclooxygenase-2 proteins were significantly upregulated in the treated seminal vesicles. The tumor necrosis factor-alpha, cyclooxygenase, interleukin 6, and inducible nitric oxide synthase mRNA expression levels were also upregulated in the treated seminal vesicles. The H2O2 levels in the seminal plasma from seminal vesicles with partial root ligation were significantly elevated compared with those from vesicle left intact. In conclusion, partially ligating the root of the seminal vesicle via sutures in rats is an effective method to establish a seminal vesiculitis rat model.

关键词： cyclooxygenase-2 rat model seminal vesiculitis tumor necrosis factor

A Novel Impact Tension Testing for OFHC Copper Bars under Local Strain Controlled

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Chinese Physics Letters 2011年第1期28卷 156-159页

作者： MA Dong-Fang HOU Yan-Jun CHEN Da-Nian WU Shan-xing wang huan-Ran Mechanics and Materials Science Research Center Ningbo University Ningbo 315211

A novel high-speed tensile facility （HSTF） with special fixture is applied to research the dynamic failure char- acteristics of oxygen-free higfl-conductivity （OFHC） copper bars at different levels of strain. The experimental tests are numerically simulated involving void evolution, It is indicated that the localized strains at necking region computed with the adjusted Johnson Cook model and the Zerilli Armstrong model are consistent with the experimental results.

关键词： Condensed matter: structural mechanical & thermal

Association of polymorphisms of A260G and A386G in DAZL gene with male infertility： a meta-analysis and systemic review

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Asian Journal of Andrology 2016年第1期18卷 96-101页

作者： Ping Chen Xiao wang Chang Xu He Xiao Wen-Hao Zhang xing-huan wang Xin-Hua Zhang Department of Urology Zhongnan Hospital of Wuhan University Wuhan 430071 China

To investigate the association of single nucleotide polymorphism 260 and 386 （SNP260 and SNP386） gene with male infertility, an electronic search was performed to identify case-control studies evaluating the relationship of SNP260 or SNP386 of deleted in azoospermia-like （DAZL） and male infertility. Review Manager 5 was used to process the meta-analysis and other statistical analysis. A total of 139 records were retrieved, of which 13 case-control studies with total 2715 patients and 1835 normozoospermic men were included. SNP260 was found not to play a functional role in male oligo/azoospermia either for Caucasians or for Asians. But for SNP386, models of allele （A/G）, dominant （AA/AG ＋ GG）, co-dominant （AA/AG） and super-dominant （AA ＋ GG/AG） had a strong correlation to spermatogenic failure with related odds ratio being 0.15 （95% confidence interval [95% CI] 0.07 to 0.34, P〈 0.00001）, 0.16 （95% CI 0.07 to 0.35, P〈 0.00001）, 0.15 （95% CI 0.06 to 0.33, P〈 0.00001） and 0.15 （95% CI 0.06 to 0.33, P 〈 0.00001）, respectively. Moreover, this correlation was only found in the Chinese Han population （decreasing around 85% risk of oligo/azoospermia infertility） and not found in India, Japan, and Caucasian countries. Our analysis demonstrated that SNP260 of DAZL did not contribute to oligo/azoospermia while SNP386 was correlated to male infertility. However, this correlation was only found in China with a country-specific and ethnicity-specific manner.

关键词： deleted in azoospermia-like male infertility meta-analysis polymorphism single nucleotide polymorphisms

Cavernous nerve reconstruction with autologous vein graft and platelet-derived growth factors

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Asian Journal of Andrology 2017年第3期19卷 298-302页

作者： Xie-Gang Ding Shi-Wen Li Xin-Min Zheng xing-huan wang Yi Luo Department of Urology Zhongnan Hospital Wuhan University Wuhan 430071 China.

In this study, we investigated the feasibility of using autologous vein graft and platelet-derived growth factors to bridge transected cavernous nerve in a rat model. A short defect in the bilateral cavernous nerve was created and repaired with vein graft from the right jugular vein or vein graft plus platelet-derived growth factors. The 32 rats were divided into four groups, namely Group 1 - no repair as a negative control, Group 2 - vein graft alone, Group 3 - vein graft plus platelet-derived growth factors, and Group 4 - sham operation as a positive control. We evaluated nerve regeneration and functional recovery using retrograde tracing study with FluoroGold, Toluidine blue staining of cavernous nerve, and the intracavernous pressure at 3 months. Three months after surgery, rich FluoroGold-positive cells were observed in the sham and vein graft plus platelet-derived growth factors group, but very few were found in the no repair group. The number of myelinated axons of regenerated cavernous nerve and intracavernous pressure were increased obviously in the two vein graft groups, especially in the vein graft plus platelet-derived growth factors group. These findings confirm the feasibility of using autologous vein as guides for cavernous nerve regeneration, and the regeneration can be further enhanced when the vein is filled with Dlatelet-derived growth factors.

关键词： cavernous nerve nerve regeneration platetet-rich plasma vein graft

Aspartoacylase suppresses prostate cancer progression by blocking LYN activation

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Military Medical Research 2024年第2期11卷 180-205页

作者： Hong Weng Kang-Ping Xiong wang wang Kai-Yu Qian Shuai Yuan Gang wang Fang Yu Jun Luo Meng‑Xin Lu Zhong‑Hua Yang Tao Liu xing huang Hang Zheng xing-huan wang Department of Urology Zhongnan Hospital of Wuhan UniversityWuhan 430071China Center for Evidence‑Based and Translational Medicine Zhongnan Hospital of Wuhan UniversityWuhan 430071China Department of Biological Repositories Zhongnan Hospital of Wuhan UniversityWuhan 430071China Hubei Key Laboratory of Tumor Biological Behaviors Wuhan 430071China Department of Pathology Zhongnan Hospital of Wuhan UniversityWuhan 430071China Center for Pathology and Molecular Diagnostics Wuhan UniversityWuhan 430071China Wuhan Research Center for Infectious Diseases and Cancer Chinese Academy of Medical SciencesWuhan 430071China

Background:Globally,despite prostate cancer(PCa)representing second most prevalent malignancy in male,the precise molecular mechanisms implicated in its pathogenesis remain ***,elucidating the key molecular regulators that govern disease progression could substantially contribute to the establishment of novel therapeutic strategies,ultimately advancing the management of ***:A total of 49 PCa tissues and 43 adjacent normal tissues were collected from January 2017 to December 2021 at Zhongnan Hospital of Wuhan *** advanced transcriptomic methodologies were employed to identify differentially expressed mRNAs in *** expression of aspartoacylase(ASPA)in PCa was thoroughly evaluated using quantitative real-time PCR and Western blotting *** elucidate the inhibitory role of ASPA in PCa cell proliferation and metastasis,a comprehensive set of in vitro and in vivo assays were conducted,including orthotopic and tumor-bearing mouse models(n=8 for each group).A combination of experimental approaches,such as Western blotting,luciferase assays,immunoprecipitation assays,mass spectrometry,glutathione S-transferase pulldown experiments,and rescue studies,were employed to investigate the underlying molecular mechanisms of ASPA's action in *** Student‘s t-test was employed to assess the statistical significance between two distinct groups,while one-way analysis of variance was utilized for comparisons involving more than two groups.A two-sided P<0.05 was deemed to indicate statistical ***:ASPA was identified as a novel inhibitor of PCa *** expression of ASPA was found to be significantly down-regulated in PCa tissue samples,and its decreased expression was independently associated with patients’prognosis(HR=0.60,95%CI 0.40–0.92,P=0.018).Our experiments demonstrated that modulation of ASPA activity,either through gain-or loss-of-function,led to the suppression or enhancement of PCa cell proliferation,mig

关键词： Prostate cancer Aspartoacylase LYN JNK AP-1 C-Jun Phosphorylation

Downregulation of cold-inducible RNA-binding protein activates mitogen-activated protein kinases and impairs spermatoRenic function in mouse testes

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Asian Journal of Andrology 2012年第6期14卷 884-889页

作者： Zhi-Ping Xia Xin-Min Zheng Hang Zheng Xiao-Jun Liu Gui-Yong Liu xing-huan wang Department of Urology Zhongnan Hospital of Wuhan University Wuhan 430071 China Department of Urology Qianjiang Central Hospital Qianjiang 433100 China

Cold-inducible RNA-binding protein （CIRP） is an RNA-binding protein that is expressed in normal testes and downregulated after heat stress caused by cryptorchidism, varicocele or environmental temperatures. The purpose of this study was to investigate the functions of CIRP in the testes. We employed RNAi technique to knock down the expression of CIRP in the testes, and performed haematoxylin and eosin staining to evaluate morphological changes following knockdown. Germ cell apoptosis was examined by terminal deoxynucleotidal transferase-mediated dUTP nick end labelling （TUNEL） assay, and mitogen-activated protein kinase （MAPK） signalling pathways were investigated by Western blotting to determine the possible mechanism of apoptosis. We found that using siRNA is a feasible and reliable method for knocking down gene expression in the testes. Compared to controls, the mean seminiferous tubule diameter （MSTD） and the thickness of the germ cell layers decreased following siRNA treatment, whereas the percentage of apoptotic seminiferous tubules increased. The p44/p42, p38 and SAPK/JNK MAPK pathways were activated after downregulation of CIRP. In conclusion, we discovered that downregulation of CIRP resulted in increased germ cell apoptosis, possibly viathe activation of the p44/p42, p38 and SAPK/JNK MAPK pathways.

关键词： cold-inducible RNA-binding protein (CIRP) mitogen-activated protein kinase (MAPK) siRNA in vivo spermatogenesis heat stress male infertility

Oral microbiota in the oral-genitourinary axis:identifying periodontitis as a potential risk of genitourinary cancers

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Military Medical Research 2021年第4期8卷 564-578页

作者： Shuai Yuan Cheng Fang Wei-Dong Leng Lan Wu Bing-Hui Li xing-huan wang Hailiang Hu Xian-Tao Zeng Center for Evidence-Based and Translational Medicine Zhongnan Hospital of Wuhan UniversityWuhan 430071China. Department of Stomatology Taihe HospitalHubei University of MedicineShiyan 442000China. Department of Stomatology Zhongnan Hospital of Wuhan UniversityWuhan 430071China. Department of Urology Zhongnan Hospital of Wuhan UniversityWuhan 430071China. Department of Pathology Duke University School of MedicineDurhamNC 27710USA. School of Medicine Southern University of Science and TechnologyShenzhen 518055GuangdongChina.

Periodontitis has been proposed as a novel risk factor of genitourinary cancers:although periodontitis and genitourinary cancers are two totally distinct types of disorders,epidemiological and clinical studies,have established associations between *** of oral microbiota has already been established as a major factor contributing to *** emerging epidemiological evidence and the detection of oral microbiota in genitourinary organs indicate the presence of an oral-genitourinary axis and oral microbiota may be involved in the pathogenesis of genitourinary ***,oral microbiota provides the bridge between periodontitis and genitourinary *** have carried out this narrative review which summarizes epidemiological studies exploring the association between periodontitis and genitourinary *** have also highlighted the current evidence demonstrating the capacity of oral microbiota to regulate almost all hallmarks of cancer,and proposed the potential mechanisms of oral microbiota in the development of genitourinary cancers.

关键词： Oral microbiota Oral-genitourinary axis Periodontitis Urogenital neoplasms Prostatic neoplasms Kidney neoplasms Urinary bladder neoplasms

Distribution profiles of transient receptor potential melastatin-related and vanilloid-related channels in prostatic tissue in rat

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Asian Journal of Andrology 2007年第5期9卷 634-640页

作者： Huai-Peng wang Xiao-Yong Pu xing-huan wang Department of Urology Guangdong Provnicial People＇s Hospital Guangzhou 510080 China

Aim： To investigate the expression and distribution of the members of the transient receptor potential （TRP） channel members of TRP melastatin （TRPM） and TRP vanilloid （TRPV） subfamilies in rat prostatic tissue. Methods： Prostate tissue was obtained from male Sprague-Dawley rats. Reverse transcription polymerase chain reaction （RT-PCR） and quantitative real-time polymerase chain reaction （PCR） were used to check the expression of all TRPM and TRPV channel members with specific primers. Immunohistochemistry staining for TRPM8 and TRPV1 were also performed in rat tissues. Results： TRPM2, TRPM3, TRPM4, TRPM6, TRPM7, TRPMS, TRPV2 and TRPV4 mRNA were detected in all rat prostatic tissues. Very weak signals for TRPM1, TRPVI and TRPV3 were also detected. The mRNA of TRPM5, TRPV5 and TRPV6 were not detected in all RT-PCR experiments. Quantitative real-time RT-PCR showed that TRPM2, TRPM3, TRPM4, TRPMS, TRPV2 and TRPV4 were the most abundantly expressed TRPM and TRPV subtypes, respectively. Fluorescence immunohistochemistry indicated that TRPM8 and TRPV 1 are highly expressed in both epithelial and smooth muscle cells. Conclusion： Our results demonstrate that mRNA or protein for TRPM1, TRPM2, TRPM3, TRPM4, TRPM6, TRPM7, TRPMS, TRPV1, TRPV2, TRPV3 and TRPV4 exist in rat prostatic tissue. The data presented here assists in elucidating the physiological function of TRPM and TRPV channels.

关键词： transient receptor potential channels prostate cation channels