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Dry Port-Seaport Logistics Network Construction under the Belt and Road Initiative: A Case of Shandong Province in China

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Journal of Systems Science and Systems Engineering 2021年第2期30卷 178-197页

作者： Jingci Xie Yiran Sun xin huo School of Management Shandong UniversityJi'nan 250100China CRRC Qingdao Sifang Co.Ltd Qingdao 266111China

In recent years,China's dry ports have entered into a period of rapid development,especially driven by the Belt and Road initiative(BRI).This initiative not only provides valuable opportunities,but also intensifies fierce competition between *** order to earn more profits and maintain an advantageous position,dry ports generally tend to rely on seaports to increase competitiveness and attract more goods,and seaports are also willing to cooperate with dry ports to expand their *** a large coastal province in China,Shandong Province has good maritime *** of the fierce competition among seaports inside and outside Shandong Province,port operators should make good use of the opportunity offered by BRI to make connections with the inland cities through the dry port-seaport logistics network and enhance the *** order to take an active part in the process of the BRI,forming a dry port-seaport logistics network is a win-win strategy for Shandong *** research first analyzes the impact of the Belt and Road initiative on ports of Shandong and its development,then uses complex network theory and TOPSIS method to select dry port candidate cities from BRI's important transportation *** considering economic benefits,carbon emissions and construction costs,a multi-objective optimization model is established with a construction cost preference *** the NSGA-II algorithm is used to solve the realistic *** study finds that when the construction cost increases,the transportation cost and carbon emission cost will decrease,which indicates that the dry port-seaport logistics network established under the BRI can reduce the cost of logistics transportation and environmental pollution.

关键词： Dry port Belt and Road Initiative logistics network mathematical programming

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Akt Gene Therapy Enhances Angiogenesis in Ischemic Hind Limb

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Chemical Research in Chinese Universities 2010年第3期26卷 409-414页

作者： huo xin ZHANG Qiang LIU Ying Department of Geriatrics First Affiliated Hospital China Medical University Shenyang 110001 P. R. China

Both bone marrow-derived mesenchymal stem cells（MSCs） therapy and gene therapy have been applied to animal studies and clinical trials. The goal of this study was to compare the effects of three types of MSCs transplantation. In the first part of the study, MSCs were transduced with the pEGFP-C1 and transfected with the pEGFP-C1/Akt in a model of bilateral hind limb ischemia in rats. Following gene transduction, the production of Akt and VEGF protein was more in the culture media of GFP-Akt/MSCs group than that in those of the GFP/MSCs group In the second part of the study, Sprague-Dawley rats（n=10/group） underwent surgery to create bilateral hind limb ischemia and were randomized into two groups consisting of a GFP/Akt-MSCs group（MSCs suspension, 1×10^7 MSCs/100 μL transfected pEGFP-C1/Akt） and a GFP-MSCs group（MSCs suspension, 1 × 10^7 MSCs/100 μL）. These PEGFP-C1/Akt and PEGFP-C 1 transfected MSCs suspensions were slowly infused into the adductor muscles of the rat＇s left hind limb, while the rat＇s right hind limb in the control group received an equal volume of PBS. Endpoints includes angiographic analysis, evaluation of capillary density, immunohistochemistry for von Willebrand factor （vWF）, immunodetection of Akt and VEGF protein, RT-PCR of VEGF and Akt mRNA levels in vitro and in vivo. Our data indicate that the tissue perfusion can improve capillary density and the mature of vasculature in the GFP-Akt/MSCs group compared to that in the GFP-MSCs group or control group in the rat model of bilateral hind limb ischemia. Transplantation of MSCs transfected with Akt gene may become the future therapy for hind limb ischemia.

关键词： Bone mesenchymal stem cell pEGFP-C1/Akt Hindlimb Ischemia Angiogenesis

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Light-induced nonhomogeneity and gradient bending in photochromic liquid crystal elastomers

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Science China(Physics,Mechanics & Astronomy) 2006年第5期49卷 553-563页

作者： JIN Lihua JIANG xin huo Yongzhong Department of Mechanics and Engineering Science Fudan UniversityShanghai 200433China Department of Mechanics and Engineering ScienceFudan UniversityShanghai 200433China Department of Mechanics and Engineering ScienceFudan UniversityShanghai 200433China

The recently reported opto-mechanical effect of some photochromic liquid crystal elastomers (LCEs) is studied. It is found that in such LCEs, material parameters such as the Young’s modulus and the stress-free strains will become nonhomogeneous under light irradiations. One may call them the light-induced functionally gradient materials. Analytical expressions for the dependence of the material parameters on the space varia- ble and possibly on the time variable are obtained. Exponential dependence can be derived under certain approximations. As an example, the light-induced bending of a beam is studied. Two neutral planes are found in the beam. Thus, along the thickness of the beam, there are extensions in the upper and lower parts and contractions in the middle.

关键词： opto-mechanical effect material nonhomogeneity functionally gradient materials light-induced bending

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The application of microfluidic-based technologies in the cycle of metabolic engineering

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Synthetic and Systems Biotechnology 2016年第3期1卷 137-142页

作者： Xiaoyan Ma Yi-xin huo College of Life Science Beijing Institute of Technology5 South Zhongguancun StreetHaidian DistrictBeijing100081China

The process of metabolic engineering consists of multiple cycles of design,build,test and learn,which is typically laborious and *** increase the efficiency and the rate of success of strain engineering,novel instrumentation must be ***,the control of liquid flow in microstructures,has enabled flexible,accurate,automatic,and high-throughput manipulation of cells in liquid at picoliter to nanoliter *** attributes hold great promise in advancing metabolic engineering in terms of the phases of design,build,test and *** promote the application of microfluidic-based technologies in strain improvement,this review addressed the potentials of microfluidics and the related approaches in DNA assembly,transformation,strain screening,genotyping and phenotyping,and highlighted their adaptations for single-cell *** a result,this facilitates in-depth understanding of the metabolic network,which in turn promote efficient optimization in the following cycles of strain *** together,microfluidic-based technologies enable on-chip workflow,and could greatly accelerate the turnaround of metabolic engineering.

关键词： Metabolic engineering Microfluidics High-throughput Single-cell analysis

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Developing a Transformation-Independent and Unbiased qPCR Assay to Rapidly Evaluate the Determinants of DNA Assembly Efficiency

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Engineering 2019年第4期5卷 803-810页

作者： Xiaoyan Ma xinxin Liang Yi-xin huo Key Laboratory of Molecular Medicine and Biotherapy School of Life ScienceBeijing Institute of TechnologyBeijing 100081China UCLA Institute for Technology Advancement(Suzhou) Suzhou 215123China

Synthetic biology is moving in the direction of larger and more sophisticated design,which depends heavily on the efficient assembly of genetic *** evaluation of the DNA assembly efficiency(AE)requires transformation,and the whole process requires up to 10 h and is susceptible to various *** achieve rapid and reliable determination of the AE,an alternative transformation-independent method was established using a modified quantitative polymerase chain reaction(qPCR)*** AE is represented by the proportion of the ligated fragment,which can be determined within 3 *** qPCR-based measurement was tested by the commonly used restriction ligation,Golden Gate assembly,and Gibson assembly for the assembly of two or more DNA pieces;the results correlated significantly with the AEs represented by the counting of the colony-forming units(CFUs).This method outperformed the CFU-based measurement by reducing the measuring bias and the random deviations that stem from the transformation *** method was then employed to investigate the effects of terminal secondary structures on DNA *** results revealed the major effects of the overall properties of the overlap sequence and the negative effects of hairpin structures on the AE,which are relevant for all assembly techniques that rely on homologous annealing of the terminal *** qPCR-based approach presented here should facilitate the development of DNA assembly techniques and the diagnosis of inefficient assemblies.

关键词： Assembly efficiency DNA assembly qPCR Secondary structure Transformation

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Breeding ecology of ground tits in northeastern Tibetan plateau, with special reference to cooperative breeding system

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Current Zoology 2011年第6期57卷 751-757页

作者： xin LU Ran huo Yang LI Wenbo LIAO Chen WANG Department of Zoology College of Life Sciences Wuhan University Wuhan 430072 China

Data on breeding ecology of a color-band marked population of the ground tit Parus humilis were collected in north Qinghai on the Tibetan plateau, during 2008 and 2009. In spring the birds excavated 0.8-3.2 m long nesting burrows under the ground. First-egg laying occurred between late April and late June during which a pair produced one brood. Incubation was done by female alone for 15-16 days and nestling-feeding by both sexes and helpers in any for 23-25 days. Average brood size at fledging was 5.8 （＋ 1.4 SD, 3-8） and all the 27 observed nesting attempts fledged at least one young. At the population level, brood sex ratio did not differ from 1：1. The birds are a territory-living resident, with annual resight rates being 48% （22 of 46） in adult breeders and 10% （7 of 67） in yearlings. Pairs were socially monogamous, of which 23% （9 of 40） contained one and some- times two male helpers, most likely being philopatric sons of the breeders. The formation of cooperative groups is similar to the population in central Tibet but differs from that in south Guansu where breeding ground tits exhibit a high level of annual turnover

关键词： Burrow-nesting Cooperative breeding Life history Parus humilis Tibetan plateau

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Torquer Current Analysis and an Improved Filter Design for Gyrowheel System 36

Torquer Current Analysis and an Improved Filter Design for G...

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第36届中国控制会议

作者： Yuyu Zhao Hui Zhao xin huo Yu Yao Harbin Institute of Technology

ISBN: (纸本)9781538629185

Gyrowheel is a unique attitude control device to measure external rates of the spacecraft body as well as provide momentum *** ensure the external rate sensing accuracy of Gyrowheel,study of the accurate characterization of the currents in the torque coils is performed in this *** of the current signals and the sources of them are analyzed,and an effective filtering method is proposed,which can be used for engineering ***,experimental results are presented to show that the work in this paper is practicable and effective.

关键词： Gyrowheel external rate sensing torquer current frequency spectrum analysis digital filter

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Semi-integer harmonic generation from an argon atom by bichromatic counter-rotating circularly polarized laser field

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Chinese Physics B 2020年第5期29卷 219-224页

作者： Tong Qi Xiao-xin huo Jun Zhang Xue-Shen Liu Institute of Atomic and Molecular Physics Jilin UniversityChangchun 130012China

We theoretically investigated the properties of the high-order harmonic generation from an argon atom by bichromatic counter-rotating circularly polarized(BCCP)laser *** harmonic emission processes have been illustrated by numerically solving the two-dimensional time-dependent Schr¨odinger equation of an atom in intense laser *** is found that with the decrease of the right-circularly polarized laser wavelength,the harmonic spectra are gradually splitting and the harmonic orders move towards the higher ***,the integer and semi-integer harmonic emission will be generated when the frequency ratios of right-and left-circularly polarized lasers are *** emission mechanism of the semi-integer-order harmonics has been investigated by using the rules of photon absorption and emission.

关键词： high-order harmonic generation semi-integer-order photon absorption and emission bichromatic counter-rotating circularly polarized laser field

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Autopilot Design of Missile with Input Delay Based on Self-Stable Region Approach 36

Autopilot Design of Missile with Input Delay Based on Self-S...

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第36届中国控制会议

作者： Jiping Peng xin huo Kemao Ma Jipeng Dong Jinpeng Zhang Control and Simulation Center Harbin Institute of Technology China Airborne Missile Academy

ISBN: (纸本)9781538629185

In this paper,the synthesis method,self-stable region(SSR) approach,is applied to uncertain and time delay *** chattering phenomenon is the main drawback of sliding mode control(SMC),and the response delay of actuators is a primary ***,the existence of time delay is common in real *** SSR approach is an improvement for SMC method.A simple second order system with input delay is taken as example to show the superiority of the SSR approach dealing with uncertainties and time *** contrast to the SMC method,the SSR approach avoids chattering essentially,and shows great tolerance for time *** the response delay of lateral thrusters,the SSR approach is applied to missile’s autopilot *** numerical simulation verifies that the SSR approach can succeed in tracking pitch angle command and decreasing the switching of lateral forces.

关键词： Self-Stable Region Sliding Mode Control Time Delay System Autopilot Design Blended Control

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A Practical Strategy of Unbalance Identification and Correction for 2-DOF Precision Centrifuges

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Journal of Harbin Institute of Technology(New Series) 2018年第1期25卷 29-38页

作者： xin huo Shuangpeng Zheng Yu Yao Weishan Chen Control and Simulation Center School of AstronauticsHarbin Institute of Technology School of Mechatronics Engineering Harbin Institute of Technology

Unbalance existing in the mechanical systems is one of the most common causes which leads to unexpected vibration,nonsmooth motions,uncertain dynamics and even instability. In this paper,the problem of unbalance identification and correction is investigated for the countershaft system of a precision centrifuge with two degrees of freedom. According to the characteristics of the load under test installed on the countershaft,a gradual subdivision algorithm is proposed to identify the phase of the unbalance,and its amplitude is calculated by using a space vector algorithm,where the vibration information of the mainshaft system is obtained by utilizing two axis-layout displacement transducers installed associated to the *** on ADAMS software,some numerical simulations are presented and compared,and further,the validity of the strategy is demonstrated by experimental examples.

关键词： dynamic unbalance identification and correction precision centrifuge countershaft system ADAMS

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