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Matrix metalloproteinase-9 expression and blood brain barrier permeability in the rat brain after cerebral ischemia/reperfusion injury

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Neural Regeneration Research 2008年第5期3卷 505-508页

作者： Lifang Lei xiaohong zi Qiuyun Tu Department of Neurology Third Xiangya Hospital Central South University Changsha 410083 Hunan Province China

BACKGROUND： The integrity of the blood brain barrier （BBB） plays an important role in the patho-physiological process of cerebral ischemia/reperfusion injury. It has been recently observed that metalloproteinase-9 （MMP-9） is closely related to cerebral ischemia/reperfusion injury OBJECTIVE： This study was designed to observe MMP-9 expression in the rat brain after cerebral ischemia/reperfusion injury and to investigate its correlation to BBB permeability. DESIGN, TIME AND SETTING： This study, a randomized controlled animal experiment, was performed at the Institute of Neurobiology, Central South University between September 2005 and March 2006. MATERIALS： Ninety healthy male SD rats, aged 3-4 months, weighing 200-280 g, were used in the present study. Rabbit anti-rat MMP-9 polyclonal antibody （Boster, Wuhan, China） and Evans blue （Sigma, USA） were also used. METHODS： All rats were randomly divided into 9 groups with 10 rats in each group： normal control group, sham-operated group, and ischemia for 2 hours followed by reperfusion for 3, 6, 12 hours, 1, 2, 4 and 7 days groups. In the ischemia/reperfusion groups, rats were subjected to ischemia/reperfusion injury by suture occlusion of the right middle cerebral artery. In the sham-operated group, rats were merely subjected to vessel dissociation. In the normal control group, rats were not modeled. MAIN OUTCOME MEASURES： BBB permeability was assessed by determining the level of effusion of Evans blue. MMP-9 expression was detected by an immunohistochemical method. RESULTS： All 90 rats were included in the final analysis. BBB permeability alteration was closely correlated to ischemia/reperfusion time. BBB permeability began to increase at ischemia/reperfusion for 3 hours, then it gradually reached a peak level at ischemia/reperfusion for 1 day, and thereafter it gradually decreased. MMP-9 expression began to increase at ischemia/reperfusion for 3 hours, then gradually reached its peak level 2 days after perfusion, and th

关键词： ischemia/reperfusion injury matrix metalloproteinase-9 blood brain barrier

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Sensory changes, C-and A-fiber function, and shoulder-hand syndrome in hemiplegic patients after stroke

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Neural Regeneration Research 2008年第7期3卷 760-763页

作者： Yi Yuan xiaohong zi Xian Huang Department of Neurology the Third Xiangya Hospital Central South University Changsha 410013 Hunan Province China

BACKGROUND： Clinical diagnosis of various neurological disorders involving the sensory nerves depends primarily on subjective description, which cannot be quantitatively evaluated, and is also less reproducible and specific. Quantitative sensory testing methods can overcome these shortcomings and is currently used to identify the function of the C- and A-fibers. OBJECTIVE： To apply the quantitative sensory testing method for analyzing changes in temperature sensation, cryalgesia, thermalgesia, and vibration sense on the skin surface of hemiplegic patients with post-stroke shoulder-hand syndrome, and to analyze the relationship between these changes and shoulder-hand syndrome. DESIGN, TIME AND SETTING： A non-randomized, concurrent, control study was performed at the Clinic and Inpatient Department of the Third Xiangya Hospital, Central South University, between June 2000 and April 2001. PARTICIPANTS： Thirty post-stroke, hemiplegic patients were divided into shoulder-hand syndrome and control groups, according to whether patients exhibited shoulder-hand syndrome, with 15 patients in each group. METHODS： A TSA2001 quantitative sensory testing device （Medoc, Israel） was used for quantitative sensory testing. All sensory testing employed limits, testing temperature sense on the palm thenar eminence and vibration sense on the thumb metacarpal. Cold threshold was ≤ 28 ℃, warmth threshold was ≥ 36 ℃, cold-evoked pain threshold was ≤ 5 ℃, heat-evoked pain threshold was ≥ 51 ℃, vibration threshold was ≥ 5 μm/s; if a patient met one of these items, he/she was considered to be hypoanesthesia. MAIN OUTCOME MEASURES： Cold, warm, cold-evoked pain, heat-evoked pain and vibration threshold changes on skin from the paralyzed upper extremity was measured in the shoulder-hand syndrome and control groups. RESULTS： Incidence of sensory disability in the shoulder-hand syndrome group increased more significantly than in the control group （P 〈 0.05）, with the primary manifestati

关键词： shoulder-hand syndrome stroke quantitative sensory testing

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Endothelin-3 and glial fibrillary acidic protein expression in the frontal and parietal cortex of type-2 diabetic mice following ischemia-reperfusion injury

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Neural Regeneration Research 2008年第6期3卷 638-641页

作者： Qiuyun Tu Xiangqi Tang Lingling Zhao xiaohong zi Department of Neurology Third Xiangya Hospital Central South University Changsha 410013 Hunan Province China Department of Neurology Second Xingya Hospital Central South Unersity Changsha 410011 Hunan Province China

BACKGROUND： Patients with type-2 diabetes mellitus exhibit higher levels of plasma endothelin-1 （ET-1）. However, very few reports exist regarding altered endothelin-3 （ET-3） and ET-1 concentrations in brain tissue. OBJECTIVE： To observe expression changes of ET-3 and glial fibrillary acidic protein （GFAP） in the frontal and parietal cortex of type-2 diabetic mice following ischemia-reperfusion injury, with various reperfusion durations. DESIGN, TIME AND SETTING： Randomized controlled animal study. The experiment was conducted in the Xiangya Medical College of Central South University and the Third Xiangya Hospital between February 2002 and January 2003. MATERIALS： Sixty-six, adult, male, Kunming mice, weighing （30 ± 5） g, as well as rabbit anti-ET-3 polyclonal and rabbit anti-GFAP polyclonal antibodies, were provided by the Neurobiology Institute of Second Military Medical University in Japan. METHODS： Sixty-six mice were randomly divided into five groups： diabetes mellitus （DM, n = 6）, diabetes mellitus with ischemia-reperfusion （DM/IR, n = 24）, ischemia-reperfusion （IR, n = 24）, sham operation （SO, n = 6）, and control （n = 6）. MAIN OUTCOME MEASURES： Following ischemia-reperfusion for 1, 3, 5, and 10 days, respectively, expression of ET- 3 and GFAP was immunohistochemically measured in the frontal and parietal cortex. RESULTS： All 66 mice were included in the final result analysis. In the IR and DM/IR groups, ET-3- and GFAP-positive neurons increased in the frontal and parietal cortex in response to one day reperfusion, peaked at five days, and decreased at 10 days. ET-3 and GFAP expression was significantly greater in the DM/IR group after reperfusion for 1 day compared to the IR group. However, at other time points, there were no significant differences between the two groups. CONCLUSION： Brain ischemia-reperfusion injury results in overexpression of ET-3 and activation of astrocytes. Diabetes increases the number of ET-3- and GFAP-positive astrocytes in brain ti

关键词： diabetes mellitus brain ischemia-reperfusion endothelin-3 glial fibrillary acidic protein

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MFG-E8 Alleviates Cognitive Impairments Induced by Chronic Cerebral Hypoperfusion by Phagocytosing Myelin Debris and Promoting Remyelination

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Neuroscience Bulletin 2024年第4期40卷 483-499页

作者： xiaohong Dong Zhi Zhang Xin Shu zi Zhuang Pinyi Liu Renyuan Liu Shengnan Xia Xinyu Bao Yun Xu Yan Chen Department of Neurology Nanjing Drum Tower HospitalAffiliated Hospital of Medical SchoolNanjing UniversityNanjing210008China Department of Neurology Nanjing Drum Tower Hospital Clinical College of Traditional Chinese and Western MedicineNanjing University of Chinese MedicineNanjing210008China Department of Neurology Drum Tower Hospital of Nanjing Medical UniversityNanjing210008China Jiangsu Key Laboratory for Molecular Medicine Medical School of Nanjing UniversityNanjing210008China Jiangsu Provincial Key Discipline of Neurology Nanjing210008China

Chronic cerebral hypoperfusion is one of the pathophysiological mechanisms contributing to cognitive decline by causing white matter *** phagocytosing myelin debris in a timely manner can promote remyelination and contribute to the repair of white ***,milk fat globule-epidermal growth factor-factor 8(MFG-E8),a microglial phagocytosis-related protein,has not been well studied in hypoperfusion-related cognitive *** found that the expression of MFG-E8 was significantly decreased in the brain of mice after bilateral carotid artery stenosis(BCAS).MFG-E8 knockout mice demonstrated more severe BCAS-induced cognitive impairments in the behavioral *** addition,we discovered that the deletion of MFG-E8 aggravated white matter damage and the destruction of myelin microstructure through fluorescent staining and electron ***,MFG-E8 overexpression by AAV improved white matter injury and increased the number of mature oligodendrocytes after ***,in vitro and in vivo experiments showed that MFG-E8 could enhance the phagocytic function of microglia via theαVβ3/αVβ5/Rac1 pathway and IGF-1 production to promote the differentiation of oligodendrocyte progenitor cells into mature ***,we found that MFG-E8 was mainly derived from astrocytes,not *** findings suggest that MFG-E8 is a potential therapeutic target for cognitive impairments following cerebral hypoperfusion.

关键词： White matter injury Cognitive dysfunction MFG-E8 Remyelination Microglial phagocytosis

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Clinical classification and gene mutation of Chinese probands with Charcot-Marie-Tooth disease Analysis of 57 cases

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Neural Regeneration Research 2011年第9期6卷 706-711页

作者： Ruxu Zhang Xiaobo Li xiaohong zi Shunxiang Huang Fufeng Zhang Kun Xia Qian Pan Beisha Tang Department of Neurology the Third Xiangya Hospital Central South University Changsha 410013 Hunan Province China Department of Neurology Xiangya Hospital Central South University Changsha 410008 Hunan Province China National Key Laboratory of Medical Genetics Central South University Changsha 410078 Hunan Province China

Charcot-Marie-Tooth （CMT） disease is the most common inherited peripheral neuropathic disorder. CMT is clinically and genetically heterogeneous. To date, 27 genes associated with the disease have been cloned. The present study carried out clinical classification according to clinical, electrophysiological and pathological features, conducted inheritance classification according to inheritance patterns, and performed mutation analysis of 13 CMT disease genes （PMP22, CX32, HSPB1, MNF2, MPZ, HSPB8, GDAP1, NFL, EGR2, SIMPLE, RAB7, LMNA, MTMR2） in 57 Chinese probands with CMT. Five cases of AD-CMT1 and 13 cases of sporadic CMT1 were diagnosed as CMT1A; five cases of X-CMT1, one case of X-CMT2 and one case of sporadic CMT1 were diagnosed as CMTXl; four cases of AD-CMT2 were diagnosed as CMT2F; one case of AD-CMT2 and one case of sporadic CMT2 were diagnosed as CMT2A2; one case of AD-CMT2 was diagnosed as CMT2L; one case of AD-CMT2 was diagnosed as CMT2J; one case of AR-CMT1 was diagnosed as CMT4A. Among the 57 CMT probands, seven genotypes were determined among 34 patients, with a detection rate of 59.6%. The results indicated that the clinical classification and inheritance classification are indispensable for selecting potential disease genes for mutation detection, and for efficient molecular diagnosis.

关键词： Charcot-Marie-Tooth disease clinical classification gene mutation analysis

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A novel transgenic mouse model of Chinese CharcotMarie-Tooth disease type 2L

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Neural Regeneration Research 2014年第4期9卷 413-419页

作者： Ruxu Zhang Fufeng Zhang Xiaobo Li Shunxiang Huang xiaohong zi Ting Liu Sanmei Liu Xuning Li Kun Xia Qian Pan Beisha Tang Department of Neurology Third Xiangya HospitalCentral South University Department of Neurology Xiangya HospitalCentral South University National Key Laboratory of Medical Genetics Central South University

We previously found that the K141N mutation in heat shock protein B8 （HSPB8） was responsible for Charcot-Marie-Tooth disease type 2L in a large Chinese family. The objective of the present study was to generate a transgenic mouse model bearing the K141N mutation in the human HSPB8 gene, and to determine whether this K141NHSPB8 transgenic mouse model would manifest the clinical phenotype of Charcot-Marie-Tooth disease type 2L, and consequently be suitable for use in studies of disease pathogenesis. Transgenic mice overexpressing K141N HSPB8 were generated using K141N mutant HSPB8 cDNA cloned into a pCAGGS plasmid driven by a human cytomegalovirus expression system. PCR and western blot analysis confirmed integration of the KI41NHSPB8 gene and widespread expression in tissues of the transgenic mice. The K141N HSPB8 transgenic mice exhibited decreased muscle strength in the hind limbs and impaired motor coordination, but no obvious sensory disturbance at 6 months of age by behavioral assessment. Electrophysiological analysis showed that the compound motor action potential amplitude in the sciatic nerve was significantly decreased, but motor nerve conduction velocity remained normal at 6 months of age. Pathological analysis of the sciatic nerve showed reduced myelinated fiber density, notable axonal edema and vacuolar degeneration in K141N HSPB8 transgenic mice, suggesting axonal involvement in the peripheral nerve damage in these animals. These findings indicate that the KI4mHSPB8 transgenic mouse successfully models Charcot-Marie-Tooth disease type 2L and can be used to study the pathogenesis of the disease.

关键词： nerve regeneration peripheral nerve injury axonal injury animal models Charcot-Ma-rie-Tooth disease type 2L gene mutation pronuclear injection transgenic model small heat shockprotein B8 NSFC grant neural regeneration

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iTRAQ-based proteomics reveals the mechanism of action of Yinlai decoction in treating pneumonia in mice consuming a high-calorie diet

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Journal of Traditional Chinese Medical Sciences 2024年第1期11卷 21-32页

作者： Qianqian Li Tiegang Liu Chen Bai Xueyan Ma Hui Liu zi’an Zheng Yuxiang Wan He Yu Yuling Ma xiaohong Gu School of Traditional Chinese Medicine Beijing University of Chinese MedicineBeijing 102488China Institute of Chinese Medicine Epidemic Disease Beijing University of Chinese MedicineBeijing 102488China School of Traditional Chinese Medicine Guizhou University of Traditional Chinese MedicineGuiyang 550025China Department of Acupuncture and Mini-invasive Oncology Beijing University of Chinese Medicine Third Affiliated HospitalBeijing 100029China OCMRC Center University of OxfordOxford OX13PTUnited Kingdom

Objective:To uncover the underlying mechanisms of action of the Yinlai decoction on high-calorie dietinduced pneumonia through proteomics ***:Based on the Gene Expression Omnibus(GEO)database,lung tissue samples from normal and high-fat diet(HFD)fed mice in the GSE16377 dataset were selected as test cohorts to identify differentially expressed genes and conduct bioinformatics *** the animal experiments,mice were randomly divided into the control(N),high-calorie diet pneumonia(M),and Yinlai decoction treatment(Y)*** in the M group received high-calorie feed and a 0.5 mg/mL lipopolysaccharide solution spray for 30 min for 3 *** mice in the Y group were intragastrically administered 2 mL/10 g Yinlai decoction twice daily for 3 *** evaluation of the lung tissue was *** expressed proteins(DEPs)in the lung tissue were identified using quantitative proteomics and bioinformatics *** drug-target relationships between Yinlai decoction and core DEPs in the lung tissue were verified using AutoDock Vina and Molecular Graphics Laboratory(MGL)*** were verified by western ***:GEO data mining showed that an HFD altered oxidative phosphorylation in mouse lung *** Yinlai decoction alleviated pathological damage to lung tissue and pneumonia in mice that were fed a high-calorie diet.A total of 47 DEPs were identified between the Y and M *** analysis revealed their association with energy metabolism pathways such as the tricarboxylic acid cycle(TCA)and oxidative *** protein-protein interaction network revealed that Atp5a1,Pdha1,and Sdha were the target proteins mediating the therapeutic effects of Yinlai *** docking results suggested that the mechanism of the therapeutic effect of Yinlai decoction involves the binding of brassinolide,praeruptorin B,chrysoeriol,and other components in Yinlai decoction to ***:The Yinlai decoction allevia

关键词： Yinlai decoction High-calorie diet Pneumonia iTRAQ proteomics Energy metabolism

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Rapid genetic screening of Charcot-Marie-Tooth disease type 1A and hereditary neuropathy with liability to pressure palsies patients

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Neural Regeneration Research 2012年第32期7卷 2522-2527页

作者： Xiaobo Li xiaohong zi Lin Li Yajing Zhan Shunxiang Huang Jin Li Xuning Li Xigui Li Zhengmao Hu Kun Xia Beisha Tang Ruxu Zhang Department of Neurology Third Xiangya Hospital Central South University Changsha 410013 Hunan Province China Department of Ultrastructure School of Basic Medical Science Central South University Changsha 410078 Hunan Province China State Key Laboratory of Medical Genetics Central South University Changsha 410078 Hunan Province China

We used the allele-specific PCR-double digestion method on peripheral myelin protein 22 （PMP22） to determine duplication and deletion mutations in the proband and family members of one family with Charcot-Marie-Tooth disease type 1 and one family with hereditary neuropathy with liability to pressure palsies. The proband and one subclinical family member from the Charcot-Marie-Tooth disease type 1 family had a PMP22 gene duplication; one patient from the hereditary neuropathy with liability to pressure palsies family had a PMP22 gene deletion. Electron microscopic analysis of ultrathin sections of the superficial peroneal nerve from the two probands demonstrated demyelination and myelin sheath hyperplasia, as well as an ＇onion-like＇ structure in the Charcot-Marie-Tooth disease type 1A patient. We observed an irregular thickened myelin sheath and ＇mouse-nibbled＇-Iike changes in the patient with hereditary neuropathy with liability to pressure palsies. In the Charcot-Marie-Tooth disease type 1A patient, nerve electrophysiological examination revealed moderate-to-severe reductions in the motor and sensory conduction velocities of the bilateral median nerve, ulnar nerve, tibial nerve, and sural nerve. Moreover, the compound muscle action potential amplitude was decreased. In the patient with hereditary neuropathy with liability to pressure palsies, the nerve conduction velocity of the bilateral tibial nerve and sural nerve was moderately reduced, and the nerve conduction velocity of the median nerve and ulnar nerve of both upper extremities was slightly reduced.

关键词： Charcot-Marie-Tooth disease hereditary neuropathy with liability to pressure palsies peripheral myelin protein 22 gene mutation PCR-double digestion method myelin sheath action potentia neuropathology neural regeneration

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克拉申输入假设理论对大学英语大班教学的启示

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沈阳大学学报（社会科学版） 2013年第4期15卷 546-549页

作者：訾晓红中国药科大学外语系江苏南京211198

分析了大学英语大班教学存在的问题。介绍了克拉申输入假设理论的内涵及对大班英语教学的启示。提出大学英语大班教学可以通过与多媒体辅助教学、任务型教学模式及分级教学相结合来使学生获得高质量的可理解性输入,从而提高教学效果。

关键词：输入假设理论大学英语大班教学

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社会场域视角下“三好儿童”的内涵诠释与教育重塑

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河南师范大学学报（哲学社会科学版） 2024年第2期51卷 151-156页

作者：刘晓红訾扬州河南师范大学教育学部河南新乡453007

“三好儿童”指具有“好思想、好品行、好习惯”的青少年儿童,是继“三好学生”之后国家对人才培养提出的新要求。话语表述方式的差异,既反映出“三好儿童”内涵与外延的变化,也蕴含着时代的变迁。首先,“三好儿童”的提出是社会资本的... 详细信息

“三好儿童”指具有“好思想、好品行、好习惯”的青少年儿童,是继“三好学生”之后国家对人才培养提出的新要求。话语表述方式的差异,既反映出“三好儿童”内涵与外延的变化,也蕴含着时代的变迁。首先,“三好儿童”的提出是社会资本的象征,包含社会声望、社会符号和社会文化,即权威人士和学者的话语、政策文件以及传统教育思想。其次,“三好儿童”的内涵可以从社会场域视角进行阐释。在政治与文化场域中,品行代表着社会文明。在养成教育场域中,思想推动品行形成习惯。再次,“三好儿童”的养成是一个文化再生产的过程,在追求教育公平的前提下,从三个向度拓展“三好儿童”的养成路径:弱化资本差距,实现教育的伦理价值;悉力形塑惯习,革新弱势阶层的认知;提振场域润染,匡助教育目标的达成,最终实现再造“三好儿童”的目标。

关键词：三好儿童社会场域教育塑造

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