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USP10 Alleviates Palmitic Acid-induced Steatosis through Autophagy in HepG2 Cells

Journal of Clinical and Translational Hepatology 2023年第1期11卷 45-57页

作者： Sheng-Liang Xin xiao-Li Pan xiao-yuan xu Yan-Yan Yu Department of Infectious Diseases Peking University First HospitalBeijingChina Division of Gastroenterology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubeiChina

Background and Aims:Nonalcoholic fatty liver disease(NAFLD)is a common chronic liver disease caused by *** autophagy is closely related to NAFLD ***,ubiquitin-specific peptidase-10(USP10)was reported to ameliorate hepatic steatosis,but the underlying mechanism is still *** view of the potential effects of USP10 on autophagy,we investigated whether USP10 alleviated steatosis through ***:HepG2 cells were treated with palmitic acid(PA)to model NAFLD in *** was used to regulate USP10 level in *** regulators were used to autophagic progression in *** blotting,real-time fluorescence quantitative polymerase chain reaction,lipid drop staining and immunofluorescent staining were performed to determine the effect of USP10 on lipid ***’s t-test and Tukey’s post hoc test were used to compare the means among ***:PA induced cellular steatosis with dependance on ***10 overexpression alleviated PA-induced steatosis,restored autophagic activity,promoted autophagic flux,including synthesis and degradation of autophagosomes,and lipid-targeted *** the presence of autophagy inhibitors,the protective effectiveness of USP10 on steatosis ***,the specific inhibitor to C-jun N-terminal protein kinase-1(JNK1),DB07268,abolished USP10-induced ***,during early stage inhibition of JNK1,compensatory expression of tuberous sclerosis complex-2(TSC2)maintained *** degree of TSC2-to-JNK1 compensation was positively associated with USP10 ***,JNK1 and TSC2 were involved in the lipid-lowering effect of ***:USP10 alleviated hepatocellular steatosis in autophagy-dependent ***1/TSC2 signaling pathways were required for USP10-induced autophagy.

关键词： Autophagy Nonalcoholic fatty liver disease Steatosis Ubiquitinspecific peptidase-10 C-jun N-terminal protein kinase-1 Tuberous sclerosis complex-2

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Effects of intracoronary injection of nicorandil and tirofiban on myocardial perfusion and short-term prognosis in elderly patients with acute ST-segment elevation myocardial infarction after emergency PCI

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World Journal of Emergency Medicine 2020年第3期11卷 157-163页

作者： Guo-xiong Chen Hong-na Wang Jin-lin Zou xiao-xu yuan Department of Cardiology Zhoushan Hospital of Zhejiang ProvinceZhoushan 316000China

BACKGROUND:This study investigated the effects of the intracoronary injection of nicorandil and tirofiban on myocardial perfusion and short-term prognosis in elderly patients with acute ST-segment elevation myocardial infarction(STEMI)after emergency percutaneous coronary intervention(PCI).METHODS:Seventy-eight STEMI patients with age>65 years who underwent emergency PCI were consecutively *** patients received conventional PCI and were randomly divided into a control group and a treatment group(n=39 per group).The control group received an intracoronary injection of tirofiban followed by a maintenance infusion for 36 hours after *** treatment group received intracoronary injection of tirofiban and nicorandil,and then intravenous infusion of tirofiban and nicorandil 36 hours after *** following parameters were measured:TIMI grade,corrected TIMI frame count(c TFC),TIMI myocardial perfusion grade(TMPG),STsegment resolution(STR)rate 2 hours post-operatively,resolution of ST-segment elevation(STR)at 2 hours postoperatively,peak level of serum CK-MB,left ventricular end diastolic diameter(LVEDD)and left ventricular ejection fraction(LVEF)at 7–10 days postoperatively,and major adverse cardiac events(MACEs)in-hospital and within 30 days ***:Compared with the control group,more patients in the treatment group had TIMI 3 and TMPG 3,and STR after PCI was significantly *** treatment group also had significantly lower c TFC,lower infarction relative artery(IRA),lower peak CK-MB,and no reflow ratio after *** treatment group had significantly higher LVEDD and LVEF but lower incidence of MACEs than the control ***:The intracoronary injection of nicorandil combined with tirofiban can effectively improve myocardial reperfusion in elderly STEMI patients after emergency PCI and improve shortterm prognoses.

关键词： Acute ST-elevation myocardial infarction Elderly Emergency coronary intervention Nicorandil Tirofiban Myocardial reperfusion

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Screening of a Composite Microbial System and Its Characteristics of Wheat Straw Degradation

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Agricultural Sciences in China 2011年第10期10卷 1586-1594页

作者： LI Pei-pei WANG xiao-juan yuan xu-feng WANG xiao-fen CAO Yan-zuan CUI Zong-jun College of Agronomy and Biotechnology China Agricultural University Beijing 100193 P.R.China

To accelerate the decomposition of wheat straw directly returned to soil, we constructed a microbial system （ADS-3） from agricultural soil containing rotting straw residues using a 40-wk limited cultivation. To assess its potential use for accelerating straw decomposing, the decomposing characteristics and the microbial composition of ADS-3 were analyzed. The results indicated that it could degrade wheat straw and filter paper by 63.8 and 80%, respectively, during 15 d of incubation. Straw hemicellulose degraded dramatically 51.2% during the first 3 d, decreasing up to 73.7% by the end of incubation. Cellulose showed sustained degradation reaching 53.3% in 15 d. Peak values of xylanase and cellulase activities appeared at 3 and 11 d, with 1.32 and 0.15 U mL-1, respectively. Estimated pH averaged 6.4-7.6 during the degradation process, which approximated acidity and alkalinity of normal soils. The microbial composition of ADS-3 was stable based on denaturing gradient gel electrophoresis （DGGE） analysis. By using bacterial 16S rRNA and fungal 26S rRNA gene clone library analysis, 20 bacterial clones and 7 fungal clones were detected. Closest identified relatives of bacteria represented by Bacillus fusiformis, Cytophaga sp., uncultured Clostridiales bacterium, Ruminobacillus xylanolyticum, Clostridium hydroxybenzoicum, and uncultured proteobacterium and the fungi were mainly identified as related to Pichia sp. and uncultured fungus.

关键词： wheat straw degradation composite microbial system cellulose hemicelulose cellulase xylanase microbial composition

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De novo combination therapy with lamivudine and adefovir dipivoxil in chronic hepatitis B patients

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World Journal of Gastroenterology 2011年第43期17卷 4804-4809页

作者： xiao-Hong Fan Jian-Zhang Geng Li-Fen Wang Ying-Ying Zheng Hai-Ying Lu Jing Li xiao-yuan xu Department of Infectious Diseases Peking University First Hospital Beijing 100034 China

AIM:To investigate the appropriate time for combination therapy in HBeAg positive chronic hepatitis B(CHB) patients with decompensated ***:Thirty HBeAg positive CHB patients with decompensated cirrhosis were enrolled in the *** of the patients were given 48 wk combination therapy with lamivudine(LAM) and adefovir dipivoxil(ADV) .Briefly,10 patients were given the de novo combination therapy with LAM and ADV,whereas the other 20 patients received ADV in addition to LAM after hepatitis B virus(HBV) genetic ***:Serum alanine aminotransferase and total bilirubin were both improved in the two groups at 4,12,24 and 48 wk after *** albumin was also improved at 24 and 48 wk after combination therapy in both *** serum HBV DNA level wasstill detectable in every patient in the two groups at 4 and 12 wk after combination ***,in the de novo combination group,serum HBV DNA levels in 4(40%) and 9(90%) patients was decreased to below 1×10 3 copies/mL at 24 and 48 wk after the combination treatment,*** parallel,serum HBV DNA levels in 2(20%) and 8(40%) patients in the add-on combination group became undetectable at 24 and 48 wk after combination treatment,***,6(60%) patients in the de novo combination group achieved HBeAg seroconversion after 48 wk treatment,whereas only 4(20%) patients in the add-on combination group achieved ***-Pugh score of patients in the de novo combination group was better than that of patients in the add-on combination group after 48 wk ***,patients in the de novo combination group had a significantly decreased serum creatinine level and elevated red blood cell ***:De novo combination therapy with LAM and ADV was better than add-on combination therapy in terms of Child-Pugh score,virus inhibition and renal function.

关键词： Hepatitis B Chronic Cirrhosis Decompensated De novo combination Lamivudine Adefovir dipivoxil

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Early renal injury indicators can help evaluate renal injury in patients with chronic hepatitis B with long-term nucleos(t)ide therapy

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World Journal of Clinical Cases 2020年第24期8卷 6306-6314页

作者： Tong-Tong Ji Ning Tan Hai-Ying Lu xiao-yuan xu Yan-Yan Yu Department of Infectious Diseases Peking University First HospitalBeijing 100034China

BACKGROUND Patients with chronic hepatitis B(CHB)with long-term nucleos(t)ide therapy may experience renal *** renal function indicators,such as urine protein,serum urea nitrogen(BUN),and serum creatinine,are normal when early mild lesions ***,more sensitive renal function indicators are *** To investigate the significance of early renal injury indicators in evaluating renal injury in patients with CHB with long-term nucleos(t)ide *** We collected the clinical data of 69 outpatients with CHB at Peking University First Hospital from March 2018 to January 2020 who had been treated with longterm nucleos(t)ide therapy and analyzed the results of early renal injury *** normal distribution data were analyzed by the t-test to determine the difference between two *** non-normally distributed data were analyzed by the Mann-Whitney U-test between two *** Kruskal-Wallis H test was used to determine the differences among multiple *** data were analyzed by the chi-square *** related factors of early renal injury indicators were analyzed by logistic regression *** The average treatment duration with nucleos(t)ide analogs of the 69 patients with CHB was 99.7±28.7 *** cases of patients with elevated BUN and hypophosphatemia were 6(8.7%)and 13(18.8%),respectively;31(44.9%)patients had abnormal early renal injury indicators,including 9 patients with abnormal urine microalbumin,7 patients with abnormal urine immunoglobulin,6 patients with abnormal urine transferrin,and 19 patients with abnormalα1 *** were no significant differences in the mean values of age,sex,BUN,estimated glomerular filtration rate(eGFR),serum uric acid,serum calcium,or serum phosphorus between the two groups of patients with and without early renal injury ***,the mean levels of serum creatinine and urine creatinine,N-acetyl-β-D-glucosidase enzyme,α1 microglo

关键词： Early renal injury Chronic hepatitis B Nucleos(t)ide analog N-acetyl-β-Dglucosidase enzyme α1 microglobulin Urine immunoglobulin

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The stripper design and test at HIRFL-CSR

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Nuclear Science and Techniques 2010年第4期21卷 237-240页

作者： ZHANG Hongbin xiao Guoqing yuan Youjin xu Hushan LI Zhankui LU Ziwei MAO Ruishi ZHAO Tiecheng Institute of Modern Physics Chinese Academy of Sciences Lanzhou 730000 China

Charge stripping is employed to produce multi-charged ions for injecting the cooling storage *** penetrating through the carbon foil,the widened distribution of ion charge states poses a limit to the ion ***,the carbon foil plays a key role in the charge stripping *** this paper,four strippers for Heavy Ion Research Facility at Lanzhou(HIRFL) and Cooling Storage Ring(CSR) are *** charge state distribution of the stripped ions is measured and the stripping efficiency of the foils is *** experimental results are consistent with the theoretical values.

关键词：兰州重离子加速器 CSR 塔设计多电荷离子重离子研究装置冷却储存环电荷态分布测试

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Mutations in surface and polymerase gene of chronic hepatitis B patients with coexisting HBsAg and anti-HBs

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World Journal of Gastroenterology 2006年第26期12卷 4219-4223页

作者： Hai-Ying Lu Zheng Zeng xiao-yuan xu Nai-Lin Zhang Min Yu Wei-Bo Gong Department of Infectious Diseases Peking University First Hospital Beijing 100034 China

AIM： To investigate the clinical significance and presence of mutations in the surface （S） and overlapping polymerase gene of hepatitis B patients with coexisting HBsAg and anti-HBs. METHODS： Twenty-three patients with chronic hepatitis B were studied. Of the 23 patients, i i were both positive for hepatitis B virus （HBV） surface antigen （HBsAg） and antibody to HBV surface antigen （anti-HBs）, 12 were negative for anti-HBs while positive for HBsAg. DNA was extracted from 200 μL serum of the patients. Nucleotide of the surface and overlapping polymerase gene from HBV-infected patients was amplified by PCR, and the PCR products were sequenced. RESULTS： Forty-one mutations were found within the surface gene protein of HBV in 15 patients （10 with coexisting HBsAg and anti-HBs）. Six （14.6%） out of 41 mutations were located at ＂α＂ determinant region in 5 patients （4 positive for HBsAg and anti-HBs）. Eleven mutations （26.8%） occurred in the downstream or upstream of ＂α＂ determinant region. Lamivudine （LMV）- selected mutations were found in three patients who developed anti-HBs, which occurred in amino acid positions （196, 198, 199） of the surface protein and in YMDD motif （M204I/V） of the polymerase protein simultaneously. Presence of these mutations did not relate to changes in ALT and HBV DNA ***： Besides mutations in the ＂α＂ determinant region, mutations at downstream or upstream of the ＂α＂ determinant region may contribute to the development of anti-HBs. These mutations do not block the replicating competency of HBV in the presence of high titer of anti-HBs.

关键词： Hepatitis B virus surface antigen Antibody to hepatitis B virus surface Mutation

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Thyroid dysfunction in Chinese hepatitis C patients: Prevalence and correlation with TPOAb and CXCL10

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World Journal of Gastroenterology 2015年第33期21卷 9765-9773页

作者： Ren-Wen Zhang Cui-Ping Shao Na Huo Min-Ran Li Hong-Li Xi Min Yu xiao-yuan xu Department of Infectious Diseases Peking University First Hospital

AIM: To investigate the relationship among pretreatment serum CXC chemokine ligand 10(CXCL10),thyroid peroxidase antibody(TPOAb) levels and thyroid dysfunction(TD) in Chinese hepatitis C ***: One hundred and thirty-nine treatmentnaive genotype 1 chronic hepatitis C patients with no history of TD or treatment with thyroid hormones were enrolled in this *** underwent peginterferon alfa-2a/ribavirin(Peg IFNα-2a/RBV) treatment for 48 wk,followed by detection of clinical factors at each follow-up *** C virus(HCV) antibodies were analyzed using microsomal chemiluminescence,and serum HCV RNA was measured by real-time PCR assay at 0,4,12,24 and 48 wk after the initiation of therapy and 24 wk after the end of *** assess thyroid function,serum thyroid stimulating hormone(TSH),free thyroxine(FT4),free triodothyronine(FT3) and TPOAb/thyroglobulin antibody(TGAb) levels were determined using chemiluminescent immunoassays every 3 *** CXCL10 levels were determined at ***: The prevalence of TD was 18.0%.Twentyone(84.0%) out of twenty-five patients exhibited normal thyroid function at week 24 after *** rate of sustained virological response to Peg IFNα-2a/RBV in our study was 59.0%(82/139),independent of thyroid *** serum CXCL10 levels were significantly increased in patients with euthyroidstatus compared with patients with TD(495.2 ± 244.2 pg/m L vs 310.0 ± 163.4 pg/m L,P = 0.012).Patients with TD were more frequently TPOAb-positive than non-TD(NTD) patients(24.2% vs 12.3%,P = 0.047) at *** of the one hundred and fifteen patients without TPOAb at baseline developed TD at the end of treatment(37.5% vs 2.6%,P = 0.000).Female patients exhibited an increased risk for developing TD compared with male patients(P = 0.014).CONCLUSION: Lower pretreatment serum CXCL10 levels are associated with TD,and TD prevalence increases in female patients and patients who are positive for TPOAb at baseline.

关键词： Thyroid dysfunction Thyroid peroxidase antibody CX

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Management of chronic liver diseases and cirrhosis: current status and future directions

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Chinese Medical Journal 2020年第22期133卷 2647-2649页

作者： Jing-Hang xu Yan-Yan Yu xiao-yuan xu Department of Infectious Diseases Peking University First HospitalBeijing 100034China

Chronic liver diseases(CLD)and cirrhosis are substantial health burdens *** 2017,with an estimation of 1.5 billion cases,the age-standardized prevalence increases by 10.4%when compared with that in 2007.[1]Globally,the most common etiologies of CLD and cirrhosis are non-alcoholic fatty liver disease(NAFLD),followed by hepatitis B virus(HBV),hepatitis C virus(HCV),and alcohol liver disease(ALD)in 2017.[1]Similarly,NAFLD is a common cause of CLD and cirrhosis in China,with an estimation of 173 to 310 million cases according to recent surveys.

关键词： cirrhosis diseases NAFLD

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Serum hepatitis B virus RNA is a predictor of HBeAg seroconversion and virological response with entecavir treatment in chronic hepatitis B patients

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World Journal of Gastroenterology 2019年第6期25卷 719-728页

作者： Hao Luo Xia-Xia Zhang Li-Hua Cao Ning Tan Qian Kang Hong-Li Xi Min Yu xiao-yuan xu Department of Infectious Diseases Peking University First Hospital Department of Gastroenterology Capital Medical University Beijing Tiantan Hospital Department of Infectious Diseases the Third Hospital of Qinhuangdao

BACKGROUND Characteristics of alterations of serum hepatitis B virus(HBV) RNA in different chronic hepatitis B(CHB) patients still cannot be fully explained. Whether HBV RNA can predict HBeAg seroconversion is still *** To investigate whether HBV RNA can predict virological response or HBeAg seroconversion during entecavir(ETV) treatment when HBV DNA is *** The present study evaluated 61 individuals who were diagnosed and treated with long-term ETV monotherapy at the Department of Infectious Diseases of Peking University First Hospital(China) from September 2006 to December ***, 30 treatment-naive individuals were included. Serum HBV RNA were extracted from 140 μL serum samples at two time points. Then they were reverse transcribed to cDNA with the HBV-specific primer. The product was quantified by real-time quantitative PCR(RT-PCR) using TAMARA probes. Statistical analyses were performed with IBM SPSS *** Level of serum HBV RNA at baseline was 4.15 ± 0.90 log10 copies/mL. HBV RNA levels showed no significant difference between the virological response(VR)and partial VR(PVR) groups at baseline(P = 0.940). Serum HBV RNA significantly decreased among patients who achieved a VR during ETV therapy(P < 0.001). The levels of HBV RNA in both HBeAg-positive patients with seroconversion group and those with no seroconversion increased after 24 wk of treatment. Overall, HBV RNA significantly but mildly correlated to HBsAg(r =0.265, P = 0.041), and HBV RNA was not correlated to HBV DNA(r = 0.242, P =0.062). Furthermore, serum HBV RNA was an independent indicator for predicting HBeAg seroconversion and virological response. HBeAg seroconversion was more likely in CHB patients with HBV RNA levels below4.12 log10 copies/mL before *** The level of serum HBV RNA could predict HBeAg seroconversion and PVR during treatment. In the PVR group, the level of serum HBV RNA tends to be increasing.

关键词： Chronic hepatitis B Hepatitis B virus RNA Virological response HBeAg seroconversion Entecavir

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