USP10 Alleviates Palmitic Acid-induced Steatosis through Autophagy in HepG2 Cells
作者机构：Department of Infectious DiseasesPeking University First HospitalBeijingChina Division of GastroenterologyUnion HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubeiChina
出 版 物：《Journal of Clinical and Translational Hepatology》 (临床与转化肝病杂志（英文版）)
年 卷 期：2023年第11卷第1期
页 面：45-57页
核心收录：
学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学]
主 题：Autophagy Nonalcoholic fatty liver disease Steatosis Ubiquitinspecific peptidase-10 C-jun N-terminal protein kinase-1 Tuberous sclerosis complex-2
摘 要：Background and Aims:Nonalcoholic fatty liver disease(NAFLD)is a common chronic liver disease caused by *** autophagy is closely related to NAFLD ***,ubiquitin-specific peptidase-10(USP10)was reported to ameliorate hepatic steatosis,but the underlying mechanism is still *** view of the potential effects of USP10 on autophagy,we investigated whether USP10 alleviated steatosis through ***:HepG2 cells were treated with palmitic acid(PA)to model NAFLD in *** was used to regulate USP10 level in *** regulators were used to autophagic progression in *** blotting,real-time fluorescence quantitative polymerase chain reaction,lipid drop staining and immunofluorescent staining were performed to determine the effect of USP10 on lipid ***’s t-test and Tukey’s post hoc test were used to compare the means among ***:PA induced cellular steatosis with dependance on ***10 overexpression alleviated PA-induced steatosis,restored autophagic activity,promoted autophagic flux,including synthesis and degradation of autophagosomes,and lipid-targeted *** the presence of autophagy inhibitors,the protective effectiveness of USP10 on steatosis ***,the specific inhibitor to C-jun N-terminal protein kinase-1(JNK1),DB07268,abolished USP10-induced ***,during early stage inhibition of JNK1,compensatory expression of tuberous sclerosis complex-2(TSC2)maintained *** degree of TSC2-to-JNK1 compensation was positively associated with USP10 ***,JNK1 and TSC2 were involved in the lipid-lowering effect of ***:USP10 alleviated hepatocellular steatosis in autophagy-dependent ***1/TSC2 signaling pathways were required for USP10-induced autophagy.
维普期刊数据库