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毛细管电泳在纳米粒子分离和性质表征中的应用进展

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化学研究与应用 2008年第9期20卷 1095-1100页

作者：冯刚肖尚友夏之宁重庆大学化学化工学院重庆400030

毛细管电泳方法被成功用于分离性质不同的纳米粒子,并且可对纳米粒子的表面zeta电势、等电点及表面电荷等表面性质进行表征。本文综述了毛细管电泳法在纳米粒子的分离分析以及其表面性质研究中的应用进展。

关键词：毛细管电泳纳米粒子表面性质综述

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符号教育学论纲

高等教育研究 2017年第10期38卷 48-54页

作者：崔岐恩张晓霞岭南师范学院粤西教师教育研究中心广东湛江524048 岭南师范学院教育科学学院广东湛江524048

符号教育学是以符号学为工具研究教育现象和问题的教育流派。符号教育学者在教育符号域(教育情境和教育活动)中观察和解释符号及其意义,探究教育符号行为的心理动机、意义及其各种指号关系中的认知和释义过程,并通过对符号的研究来认知... 详细信息

符号教育学是以符号学为工具研究教育现象和问题的教育流派。符号教育学者在教育符号域(教育情境和教育活动)中观察和解释符号及其意义,探究教育符号行为的心理动机、意义及其各种指号关系中的认知和释义过程,并通过对符号的研究来认知教育现象和思考教育问题,从符号学视角描述、解释、预测、改进教育。符号教育学体系的纵向结构含原理层、规范层、实践层。符号教育学的研究重点有符号中的教育、教育中的符号、符号与教育的关系。符号教育化和教育符号化是符号教育学的两大走向。

关键词：符号学教育学符号教育符号教育学

Neuroprotective effect of isoliquriitin on corticosterone-induced apoptosis in PC12 cells

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中国药理学与毒理学杂志 2017年第5期31卷 467-467页

作者： LI xiao GONG Wen-xia ZHOU Yu-zhi QIN Xue-mei Modern Research Center for Traditional Chinese Medicine Shanxi University College of Chemistry and Chemical Engineering Shanxi University

OBJECTIVE Isoliquiritin,a flavonoid glycosides compound,possess a broad spectrum of pharmacological activities including antioxidant,antiinflammatory. However,rare studies in the field of isoliquiritin antidepressant-like effects have been carried out,the molecular mechanism also remains unclear. In this study,the model of corticosterone-damaged rat adrenal pheochromocytoma(PC12)cells has been used to generate an in vitro experimental model of depression. The aim was to investigate thecytoprotective efficiency and potential mechanisms of isoliquiritin in corticosterone-damaged PC12 cells. METHODS The cells were treated with 400 μmol·L^(-1)corticosterone in the absence or presence of isoliquiritin for 24 h; cells viability and lactate dehydrogenase(LDH) leakage were then determined,Hoechst 33342/PI and Annexin V/PI double staining were performed to evaluate the cytoprotective efficiency of isoliquiritin. Next,intracellular calcium([Ca^(2+)]_i),mitochondrial membrane potential(MMP),reactive oxygen species(ROS),malondialdehyde(MDA),the activity of superoxide dismutase(SOD)and catalase(CAT),and Western blotting analysed for the expression of Bcl,Bax,caspase-3 and cytochrome C(Cyt-C) were investigated to explore the potential mechanisms. RESULTS The results of the present study showed that pretreatment of PC12 cells with isoliquiritinsignificantly prevented the corticosterone-induced cell apoptosis. In addition,isoliquiritin increased the activity of SOD and CAT,decreased the contents of ROS and MDA. These findings suggest that isoliquiritin provided a protective action against corticosterone-induced celldamage by reducing oxidative stress. Furthermore pretreatmented with isoliquiritin reduced corticosterone-induced mitochondrial dysfunction by preventing mitochondrial membrane potentials *** findings indicated that isoliquiritin might exert its therapeutic effects viaregulating mitochondrial dysfunction. Moreover isoliquiritin strongly attenuated [Ca^(2+)]_i

关键词： isoliquiritin neurotoxicity oxidative stress mitochondria apoptotic calcium

Establishment and performance analysis of a new multiplex detection method for influenza an and B virus antigen

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World Journal of Clinical Cases 2024年第23期12卷 5338-5345页

作者： Cheng-Jing xia Bao-Hua Li Yan-Ni Guo xiao-He Zhou Run-Ling Zhang Ying-No Niu Department of Clinical Laboratory West WingShenzhen Hospital(Guangming)of University of Chinese Academy of SciencesShenzhen 518106Guangdong ProvinceChina Laboratory Nanjing Vazyme Biotech Co.LtdNanjing 210033Jiangsu ProvinceChina

BACKGROUND Influenza A and B virus detection is pivotal in epidemiological surveillance and disease *** and accurate diagnostic techniques are crucial for timely clinical intervention and outbreak *** dot-encoded microspheres have been widely used in *** integration of quantum dot-encoded microspheres with flow cytometry is a well-established technique that enables rapid ***,establishing a multiplex detection method for influenza A and B virus antigens based on flow cytometry quantum dot microspheres will help in disease *** To establish a codetection method of influenza A and B virus antigens based on flow cytometry quantum dot-encoded microsphere technology,which forms the foundation for the assays of multiple respiratory virus *** Different quantum dot-encoded microspheres were used to couple the monoclonal antibodies against influenza A and *** known influenza A and B antigens were detected both separately and simultaneously on a flow cytometer,and the detection conditions were optimized to establish the influenza A and B antigen codetection method,which was utilized for their detection in clinical *** results were compared with the fluorescence quantitative polymerase chain reaction(PCR)method to validate the clinical performance of this *** The limits of detection of this method were 26.1 and 10.7 pg/mL for influenza A and B antigens,respectively,which both ranged from 15.6 to 250000 pg/*** the clinical sample evaluation,the proposed method well correlated with the fluorescent quantitative PCR method,with positive,negative,and overall compliance rates of 57.4%,100%,and 71.6%,*** A multiplex assay for quantitative detection of influenza A and B virus antigens has been established,which is characterized by high sensitivity,good specificity,and a wide detection range and is promising for clinical applications.

关键词： Influenza A Influenza B Quantum dot microspheres Antigen detection Multiplex detection

Soil vanadium pollution and microbial response characteristics from stone coal smelting district

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Transactions of Nonferrous Metals Society of China 2015年第4期25卷 1271-1278页

作者：肖细元杨淼郭朝晖姜智超刘亚男曹霞中南大学冶金与环境学院环境工程系长沙410083

A field investigation was performed to study the content, speciation and mobility of vanadium, as well as microbial response in soil from a stone coal smelting district in Hunan province of China. The results showed that the contents of soil V ranged from 168 to 1538 mg/kg, which exceeded the maximum permissible value of Canadian soil quality for V. The mean soil V content from wasteland area reached 1421 mg/kg, and those from the areas related with slag heap, ore pile and smelting center were 380, 260 and 225 mg/kg, respectively. Based on the results of the modified BCR sequential extraction procedure, V contents in the mobile fractions varied from 19.2 to 637 mg/kg accounting for 7.4%-42.3% of total V, and those of V(+5) species were between 21.9 and 534.0 mg/kg. Soil enzyme activity and microbial basal respiration were adversely affected by high level of soil V. More attention should be paid to soil V pollution and potential hazardous surrounding the stone coal smelting district.

关键词： vanadium soil pollution mobility microbial response stone coal smelting district

两种不同型号的Ahmed阀治疗难治性青光眼的疗效评价

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眼科 2018年第5期27卷 367-371页

作者：肖红霞叶汉元湖北省荆门市第二人民医院眼科 448000

目的探讨两种不同型号Ahmed青光眼阀治疗难治性青光眼的有效性和安全性。设计回顾性病例对照研究。研究对象 2010年7月至2015年7月行Ahmed阀植入术治疗难治性青光眼的患者106例。方法根据患者植入Ahmed青光眼阀的型号分为FP8组(睑裂相... 详细信息

目的探讨两种不同型号Ahmed青光眼阀治疗难治性青光眼的有效性和安全性。设计回顾性病例对照研究。研究对象 2010年7月至2015年7月行Ahmed阀植入术治疗难治性青光眼的患者106例。方法根据患者植入Ahmed青光眼阀的型号分为FP8组(睑裂相对小的患者,46例)和FP7组(睑裂相对大的患者,60例)。患者接受FP8或者FP7Ahmed房水引流物植入术。术后1天,1周,1、3、6、12个月随访,观察并比较两组患者眼压,术后抗青光眼用药的数目,手术成功率,术后最佳矫正视力以及并发症。主要指标眼压,术后抗青光眼用药的数目,手术成功率,术后最佳矫正视力以及并发症。结果 FP8组和FP7组术前眼压分别为(39.78±7.37)mmHg和(41.68±9.60)mmHg,术后随访各时间点平均眼压分别为(15.13±7.41)mmHg和(16.32±6.94)mmHg。FP8组和FP7组术后每个随访时间点分别与术前比较,眼压均明显下降(P均<0.001)。两组之间比较,每个随访时间点差异均无统计学意义。FP8组、FP7组术前抗青光眼用药数目分别为(3.44±0.78)种和(3.35±0.66)种,术后随访各时间点用药数目均明显下降,平均分别为(0.28±0.67)种和(0.33±7.36)种(P均<0.001)。随访结束时,FP8组末次检查22眼视力不同程度提高,19眼视力不变,5眼视力下降;FP7组术后30眼视力改善,22眼视力不变,8眼视力下降。两组术后随访一年视力比较,差异无统计学意义(χ~2=0.049, P=0.824)。Kaplan-Meier生存曲线显示,随访1年时,FP8组和FP7组手术总成功率分别为69.6%和78.3%,差异无统计学意义(P=0.499)。结论 Ahmed青光眼阀植入是治疗难治性青光眼安全有效的方法。不同型号的Ahmed青光眼阀治疗难治性青光眼有效性和安全性相当。

关键词：难治性青光眼 Ahmed青光眼阀

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一种固定时间收敛模型参考终端滑模控制方法

自动化学报 2022年第3期48卷 712-723页

作者：张骁骏袁夏明王向阳朱纪洪李春文清华大学自动化系北京100084 清华大学计算机科学与技术系北京100084 清华大学精密仪器系北京100084

针对一类具有模型不确定性和外部扰动的时变非线性系统,基于模型参考控制方法,设计了具有固定时间收敛特性的终端滑模控制器.首先,提出一种带有输入饱和限幅和补偿信号滤波的模型参考控制结构;然后针对广义误差信号,采用新型终端滑模面... 详细信息

针对一类具有模型不确定性和外部扰动的时变非线性系统,基于模型参考控制方法,设计了具有固定时间收敛特性的终端滑模控制器.首先,提出一种带有输入饱和限幅和补偿信号滤波的模型参考控制结构;然后针对广义误差信号,采用新型终端滑模面设计了补偿控制器,较好地平衡靠近和远离平衡点的收敛速度.基于李雅普诺夫方法证明了闭环系统的稳定性和固定时间收敛特性,并给出了收敛时间上界.最后将该方法应用到含有极限环的非线性系统跟踪控制中,仿真结果验证了该方法的有效性.

关键词：固定时间收敛模型参考控制终端滑模控制时变非线性系统

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大肠杆菌悬浮液强化扩散传质的实验研究

工程热物理学报 2021年第9期42卷 2394-2400页

作者：刘志军程阳孜力汗刘凤霞魏炜许晓飞大连理工大学流体与粉体工程研究设计所大连116024 大连理工大学生物工程学院大连116024

实验测量罗丹明B在大肠杆菌悬浮液中的扩散系数,考察大肠杆菌悬浮液温度、浓度和细菌活性对罗丹明B扩散传质的影响。实验结果表明,罗丹明B在悬浮液中的有效扩散系数比其在磷酸缓冲盐溶液(PBS)中的扩散系数大1个数量级,且有效扩散系数随... 详细信息

实验测量罗丹明B在大肠杆菌悬浮液中的扩散系数,考察大肠杆菌悬浮液温度、浓度和细菌活性对罗丹明B扩散传质的影响。实验结果表明,罗丹明B在悬浮液中的有效扩散系数比其在磷酸缓冲盐溶液(PBS)中的扩散系数大1个数量级,且有效扩散系数随悬浮液温度升高和细菌浓度增大而增大;细菌自驱动行为是强化悬浮液中小分子扩散传质的重要因素,罗丹明B的活性扩散系数随细菌悬浮液浓度增加和温度升高而增大,活性扩散系数因细菌的集群运动会随悬浮液浓度变化发生阶跃增大;均方位移和扩散时间的对应关系表明罗丹明B的扩散在短时间内为标准菲克扩散,一段时间后转变为超扩散,且转变时间随悬浮液温度升高而延迟,随着悬浮液浓度增加而提前。

关键词：大肠杆菌悬浮液活性流体小分子扩散增强活性扩散系数

苦参碱联合丹参注射液治疗轻中度慢性乙型肝炎的临床疗效分析

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中西医结合学报 2006年第6期4卷 648-650页

作者：胡侠周岳进谢庆荣孙晓彩肖扬解放军118医院传染科浙江温州325000

慢性乙型肝炎(chronic hepatitis B,CHB)因持续的病毒感染、免疫工能紊乱,治疗比较棘手,而反复炎症后出现的肝纤维化引起肝内、外循环障碍,加重肝细胞损伤,促进肝纤维化进一步发展.

关键词：苦参碱丹参注射液乙型肝炎肝纤维化