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Topical dihydroartemisinin inhibits suture-induced neovascularization in rat corneas through ERK1/2 and p38 pathways

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International Journal of Ophthalmology(English edition) 2011年第2期4卷 150-155页

作者： Yan-Yan Zhong, xiao-he lu Department of Ophthalmology Zhujiang Hospital Southern Medical University Guangzhou 510282 Guangdong Province China Department of Febrile Disease the First Affiliated Hospital Guangzhou University of Chinese Medicine Guangzhou 510405 Guangdong Province China Department of Ophthalmology Nanfang Hospital Southern Medical University Guangzhou 510515 Guangdong Province China

AIM: To determine if topical instillation of dihydroartemisinin (DHA) inhibits corneal neovascularization (NV) in rats and to investigate the role of the extracellular regulated kinases (ERK) 1/2 and p38 pathways in this process. O METHODS: Suture-induced corneal NV was produced in rats and the eyes were topically treated with different concentrations of DHA (20mg/L, 10mg/L or 5mg/L) or normal saline 4 times a day for 7 days. The corneal NV was quantified as the proportion of NV area to the whole cornea. Western blot was used to determine the expressions of vascular endothelial growth factor (VEGF) and the phosphorylation status of VEGF receptor-2, ERK1/2 and p38 in the corneas. Immunofluorescent staining was used to determine the expressions of phospho-ERK1/2 and phospho-p38 in the corneal tissues from the eyes treated with 20 mg/L DHA (DHA group) or normal saline (control group). RESULTS: The proportion of corneal NV area in the eyes treated with normal saline or DHA at dosages of 20mg/L, 10mg/L or 5mg/L was (23.74 +/- 3.00)%, (15.73 +/- 2.88)%, (19.53 +/- 2.42)%, and (23.38 +/- 2.79)%, respectively. In the eyes treated with 20mg/L or 10mg/L DHA, the corneal NV area was significantly reduced when compared to that in eyes with normal saline (P he expressions of VEGF and phospho-VEGFR-2. Both 20mg/L and 10mg/L DHA inhibited the expressions of phospho-ERK1/2 and phospho-p38. Immunofluorescent staining further demonstrated that 20mg/L DHA lowered the Expression levels of phospho-ERK1/2 and phospho-p38 in the corneas with suture-induced NV. O CONCluSION: Suture-induced NV in rat corneas was significantly inhibited by topical treatment with 20mg/L and 10mg/L DHA. The results suggest that the effects could be partially dependent on the DHA-mediated inhibitions of the ERK1/2 and p38 pathways.

关键词： cornea neovascularization dihydroartemisinin extracellular regulated kinases 1/2 p38

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Proteomic analysis of human serum from diabetic retinopathy

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International Journal of Ophthalmology(English edition) 2011年第6期4卷 616-622页

作者： Yin-Ping Liu, xiao-he lu Department of Ophthalmology Zhujiang Hospital Southern Medical University Guangzhou 510282 Guangdong Province China Department of Pathophysiology Southern Medical University Guangzhou 510515 Guangdong Province China Department of Ophthalmology Yijishan Hospital Wannan Medical College Wuhu 241001 Anhui Province China

AIM: To establish and compare serum proteomic of diabetic retinopathy (DR) patients in various phases and discuss pathogenesis of DR so as to find out possible serum specific molecular markers for early diagnosis of DR. METHODS: Thirty-two subjects were divided into four groups: one group of eight type 2 diabetes mellitus (T2DM) patients without apparent DR (No-DR, NDR), one group of eight T2DM patients with non-proliferative diabetic retinopathy (NPDR), one group of eight T2DM patients with proliferative diabetic retinopathy(PDR) and one group of eight healthy volunteer participants. Two dimensional fluorescence difference gel electrophoresis (2D-DIGE) was applied to establish differential protein expression profiles in four groups. Matrix-assisted laser desorption/ionization time of flight tandem mass spectrometry (MALDI-TOF-TOF MS) was applied to identify mass spectrometry of differential proteins and analyze follow-up bioinformatics. RESULTS: 2D-DIGE maps of serum protein were satisfactory obtained from NDR, NPDR, PDR and normal control groups. Twenty-six different proteins spots were screened(the volume ratio was > 1.5 based on DeCyder software analysis). Twenty-four of them were verified and two of them were not. Fifteen proteins were verified. Most of them were high-abundant proteins in serum. The four relatively low-abundant ones were beta 2-glycoprotein I (beta (2)-GPI), alpha2-HS-glycoprotein (AHSG), alpha1-acid glycoprotein (alpha(1)-AGP) and apolipoprotein A-1 (apo A-1). beta (2)-GPI expression was gradually increased in the development of DR but unrelated to the severity of DR. The volume ratio of beta (2)-GPI is 1.54, 2.43, and 2.84 in NDR, NPDR and PDR group respectively compared with normal control group. CONCluSION: Serum proteomic analysis of 2D-DIGE combined with MALDI-TOF-TOF MS is feasible to be applied in the study of DR. 13 2-GPI probably takes part in the process of DR occurrence and development and it could be a candidate biomarker on DR dia

关键词： diabetic retinopathy difference gel electrophoresis β2-glycoprotein I proteomics serum type 2 diabetes

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Concentration change of TGF-β1 in aqueous humor of rabbits

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Asian Pacific Journal of Tropical Medicine 2014年第3期7卷 241-243页

作者： xiao-Yi Yu Wei Wu xiao-he lu Department of Ophthalmology Southern Medical University Department of Ophthalmology Wuyi Traditional Chinese Medicine Hospital

Objective:To observe the influence of the the transforming growth factorβ1(TGF-β1)eve drops on rabbit aqueous humor TGF-β1 concentration,and to analyze the best drug ***:A total of 30 New Zealand white rabbits were randomly divided into5 groups with 6 in *** in control group had PBS eye drops,group A,B.C,D adopted TGF-β1 eye drops at 0.5,1.***.2.0.4.0 mg/L,respectively.4 times a *** humor of right eye was extracted 1 week after administration lo delect concentration changes of TGF-β1 by ELISA;rabbits in fpur hroups adopted 2.0 mg/L eye drops to left eyes 4 times a day,0.2 mL aqueous humor was extracted left eye at the scheduled time point 0,30 min,2 h,4 h,24 h for testing,the slit lamp was used to observe the cornea,chamber and ***:No obvious pathological changes in conjunctiva,cornea,rabbit conjunctival,anterior chamber,and the lens was *** of TGF-β1 in rabbit aqueous humor in C.D group was significantly higher than the control group(Plusions:TGF-β1 eye drops at 2.0 mg/L.4.0 mg/L can significantly increase concentration of TGF-β1 in rabbit aqueous humor,withe good ocular surfac permeability.

关键词： TGF-β1 eye drops Aqueous humor Transforming growth factor

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Choroidal thickness in pregnant women : a cross-sectional study

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International Journal of Ophthalmology(English edition) 2016年第8期9卷 1200-1206页

作者： Ru Liu Guo-Ping Kuang Di-Xian luo xiao-he lu Department of Ophthalmology Zhujiang Hospital of Southern Medical University Department of Ophthalmology the First People's Hospital of Chenzhou Institute of Translational Medicine the First People's Hospital of Chenzhou

AIM: To investigate choroidal thickness in pregnant women and compare the measurements with those of normal nonpregnant women. METHODS: Using enhanced depth imaging optical coherence tomography (EDI-OCT), choroidal thickness was measured at the fovea and at 1 mm and 3 mm superior, inferior, temporal, and nasal to the fovea in both healthy pregnant women and nonpregnant women. Pearson correlation analysis was performed to evaluate the relationships between subfoveal choroidal thickness (SFCT) and the demographic and ocular parameters. Pooled odds ratio (OR) and 95% confidence interval (CI) were calculated using fixed-effects model when Meta analyses were conducted. RESULTS: Comparison of choroidal thickness between the groups showed that it was significantly greater in healthy pregnant women's eyes than in normal nonpregnant women's eyes at all locations except at 3 mm superior and 3 mm temporal from the fovea (Phe mean SFCT was 344.13 +/- 50.94 mu m in healthy pregnant women's eyes and 315.03 +/- 60.57 mu m in normal nonpregnant women's eyes, with a statistically significant difference (P=0.008). Pearson correlation analysis showed that age and axial length were significantly related to SFCT in healthy pregnant women, normal nonpregnant women, and all subjects. The results of our cross-sectional study were consistent with the results of the further Meta analysis, with a pooled weighted mean difference (WMD) of 33.66 mu m (95% CI: 26.16 to 41.15) for SFCT. CONCluSION: Our results, along with the comprehensive Meta-analysis, suggest that choroidal thickness in healthy pregnant women is greater than that in normal nonpregnant women.

关键词： choroidal thickness pregnant optical coherence tomography

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The prognosis of trabeculectomy in primary angleclosure glaucoma patients

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International Journal of Ophthalmology(English edition) 2019年第1期12卷 66-72页

作者： Jun-Dong Zhu Li-Lian Xie Zhi-Yuan Li xiao-he lu Department of Ophthalmology Zhujiang Hospital of Southern Medical University Department of Ophthalmology the First People’s Hospital of Chenzhou CitySouthern Medical University

AIM: To evaluate whether the level of thrombospondin-1(TSP-1) in aqueous humor can predict the prognosis of trabeculectomy in patients with primary angle-closure glaucoma(PACG). METHODS: This case-control study involved 26 patients with PACG who experienced a failed trabeculectomy(case group) and 78 age-and sex-matched patients with PACG who underwent successful trabeculectomy(control group). Aqueous humor was collected at the time of trabeculectomy and tested for TSP-1 and TGF-β2 levels with an enzyme-linked immunosorbent assay method. Logistic regression modeling was used to assess the risk factors for failed trabeculectomy. RESULTS: The mean TSP-1 aqueous concentrations were significantly higher in the case group(20.67±9.79 ng/mL) than the control group(5.17±2.29 ng/mL)(Phe transforming growth factor-β2(TGF-β2) aqueous concentrations were significantly different between the case and control group, at 3633.25 and 1090.24 pg/mL, respectively(PluSION: The aqueous humor TSP-1 and TGF-β2 levels are higher in PACG eyes with failed trabeculectomy than with successful trabeculectomy at one year. The aqueous humor TSP-1 level is an independent risk factor associated with failed trabeculectomy.

关键词： thrombospondin-1 prognosis primary angleclosure glaucoma

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Low concentration of sodium hyaluronate temporarily elevates the tear film lipid layer thickness in dry eye patients with lipid deficiency

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International Journal of Ophthalmology(English edition) 2018年第3期11卷 389-394页

作者： Yan Li Xuan Sang Liu Yang xiao-Ran Wang Jia-Hui Liu Xiong-Jun he Ying Liu xiao-he lu Zhi-Chong Wang State Key Laboratory of Ophthalmology Zhongshan Ophthalmic Center Sun Yat-sen University Guangzhou 510060 Guangdong Province China Department of Ophthalmology Zhujiang Hospital of Southem Medical University Guangzhou 510282 Guangdong Province China

AIM： To investigate the effects of different concentrations of artificial tears on lipid layer thickness （LLT） and blink rate （BR） in dry eye patients. METHODS： This study included 106 eyes of 58 patients with dry eye. The lipid deficiency type was defined as the LLT baseline 〈75 nm. The LLT and BR were measured at baseline and 1, 5 and 15min after the instillation of 0.1% or 0.3% sodium hyaluronate （SH） eye drops by using the LipiView ocular surface interferometer. RESULTS： In the lipid deficiency group, the LLT increased from baseline at 1rain post instillation. The LLT after the instillation of 0.1% SH was significantly higher than that after the instillation of 0.3% SH （P〈0.001）. The LLT returned to baseline at 15min post instillation of 0.1% SH and at 5min post instillation of 0.3% SH. In the non-lipid deficiency group, the LLT decreased from baseline at lmin and returned to baseline at 5rain for both treatments. The BRs were not significantly different at different time points for both treatments. CONCluSION： SH eye drops induce a short-term increase in LLT of patients with lipid deficiency. A low concentration of artificial tears have a stronger effect than a high concentration of artificial tears on the increase in LLT. in comparison, SH eye drops induce a transient and slight decrease in LLT of patients without lipid deficiency. A low concentration of artificial tears might be better for patients with lipid deficiency.

关键词： tear film lipid layer thickness dry eye artificialtears ocular surface interferometer sodium hyaluronate

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Spontaneous regression of retinopathy of prematurity:incidence and predictive factors

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International Journal of Ophthalmology(English edition) 2013年第4期6卷 475-480页

作者： Rui-Hong Ju Jia-Qing Zhang xiao-Yun Ke xiao-he lu Li-Fang Liang Wu-Jun Wang Department of Ophthalmology Zhujiang HospitalSouthern Medical University Department of Cardiothoracic Surgery Zhujiang HospitalSouthern Medical University Department of Cardiothoracic Surgery Nanfang HospitalSouthern Medical University

AIMTo evaluate the incidence of spontaneous regression of changes in the retina and vitreous in active stage of retinopathy of prematurity(ROP) and identify the possible relative factors during the regression.

关键词： retinopathy of prematurity spontaneous regression incidence duration of ROP

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Recent Strategies and Advances in Hydrogel‑Based Delivery Platforms for Bone Regeneration

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Nano-Micro Letters 2025年第3期17卷 389-439页

作者： xiao Wang Jia Zeng Donglin Gan Kun Ling Mingfang he Jianshu Li Yongping lu Scientific and Technological Innovation Center for Biomedical Materials and Clinical Research Guangyuan Key Laboratory of Multifunctional Medical HydrogelGuangyuan Central HospitalGuangyuan 628000People’s Republic of China Jiangsu Collaborative Innovation Center of Biomedical Functional Materials Jiangsu Key Laboratory of Bio‑Functional MaterialsSchool of Chemistry and Materials ScienceNanjing Normal UniversityNanjing 210023People’s Republic of China College of Polymer Science and Engineering State Key Laboratory of Polymer Materials EngineeringSichuan UniversityChengdu 610065People’s Republic of China

Bioactive molecules have shown great promise for effectively regulating various bone formation processes,rendering them attractive therapeutics for bone ***,the widespread application of bioactive molecules is limited by their low accumulation and short half-lives in *** have emerged as ideal carriers to address these challenges,offering the potential to prolong retention times at lesion sites,extend half-lives in vivo and mitigate side effects,avoid burst release,and promote adsorption under physiological *** review systematically summarizes the recent advances in the development of bioactive molecule-loaded hydrogels for bone regeneration,encompassing applications in cranial defect repair,femoral defect repair,periodontal bone regeneration,and bone regeneration with underlying ***,this review discusses the current strategies aimed at improving the release profiles of bioactive molecules through stimuli-responsive delivery,carrier-assisted delivery,and sequential ***,this review elucidates the existing challenges and future directions of hydrogel encapsulated bioactive molecules in the field of bone regeneration.

关键词： Hydrogel Bone regeneration Bioactive molecules Drug delivery Nano-/microscale carriers

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Efficacy of the WINROP algorithm for retinopathy of prematurity screening in Southern China

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International Journal of Ophthalmology(English edition) 2021年第1期14卷 127-132页

作者： Yi-Chen Bai Rong Wu Si-Zhe Chen Shi-Yu Wei Hui-Jie Chen Yan-Chen Chen Song-Fu Feng xiao-he lu Department of Ophthalmology Zhujiang HospitalSouthern Medical UniversityGuangzhou 510282Guangdong ProvinceChina Department of Ophthalmology Liuzhou People’s HospitalLiuzhou 545006Guangxi Zhuang Autonomous RegionChina Department of Pediatric Zhujiang HospitalSouthern Medical UniversityGuangzhou 510282Guangdong ProvinceChina

AIM:To evaluate the predicting efficacy of severe retinopathy of prematurity(ROP)by the WINROP algorithm(http://***)in Southern ***:All preterm infants with the gestational age(GA)less than 32 wk were *** ROP screening results and serial postnatal body weight were analysed *** body weight was entered into and measured by the WINROP *** outcomes were analysed,and the sensitivity,specificity,positive predictive value and negative predictive value(NPV)were ***:Totally 432 infants with a median GA of 30.0(24.0-31.9)wk,and a median birth weight(BW)of 1360(540-2700)g were *** these 432 infants,50 were diagnosed as type 1 ROP but only 28 were identified by the WINROP *** sensitivity was 56%(28/50)and the NPV was 92%(252/274).However,for infants with BWhe sensitivity was 93.8%(15/16)and 93.3%(14/15),***,with several postnatal complications added as additional risk factors,the sensitivity was increased to 96%(48/50).CONCluSION:The sensitivity of the WINROP algorithm from the Southern Chinese cohort is not as high as that reported in developed *** algorithm is effective for detecting severe ROP from extremely small or preterm *** of the algorithm with additional risk factors could improve the predictive value for infants with a GA>28 wk in China.

关键词： retinopathy of prematurity WINROP preterm infants

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未找到明确裂孔的原发性视网膜脱离的术式选择

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中国实用眼科杂志 2007年第2期25卷 198-200页

作者：何晓璐卢宁福建省级机关医院福建350003 北京市同仁眼科中心北京

目的探讨孔源性视网膜脱离术前找不到裂孔如何选择手术方式。方法回顾分析2001年1月～2004年3月我院收治的屈光间质较透明，瞳孔能散大、PVRC3级以下的原发性视网膜脱离患者，选择巩膜扣带术及玻璃体视网膜手术的术前术后视力及视网膜... 详细信息

目的探讨孔源性视网膜脱离术前找不到裂孔如何选择手术方式。方法回顾分析2001年1月～2004年3月我院收治的屈光间质较透明，瞳孔能散大、PVRC3级以下的原发性视网膜脱离患者，选择巩膜扣带术及玻璃体视网膜手术的术前术后视力及视网膜复位情况。结果54例54眼中，33例行巩膜扣带术，第一次手术解剖复位率有84．8％（29／33眼），21例行玻璃体视网膜手术，第一次手术解剖复位率有90．5％（19／21只眼），两组第一次手术解剖复位率在统计学上无显著性差异（p=0．437）。在巩膜扣带术组和玻璃体视网膜手术组两组术前术后矫正视力无显著性差异（p=0．392）。术后随访（3～27）月，平均8．5月，巩膜扣带术组并发症明显少于玻璃体视网膜手术组。结论对未找到明确裂孔的孔源性视网膜脱离患者。尽可能用损伤最小、操作简单的巩膜扣带术来获得最大成功机会。

关键词：视网膜脱离裂孔

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