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基于高效聚合特征增强网络的合成孔径雷达船舰检测方法

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光学学报 2024年第12期44卷 309-319页

作者：单慧琳刘文星王兴涛付相为李长帅张银胜南京信息工程大学电子与信息工程学院江苏南京210044 无锡学院电子信息工程学院江苏无锡214105

合成孔径雷达(SAR)由于散射效应以及波长和天线尺寸的分辨率限制,难以获取小尺寸目标的细节和边界信息,因此,检测准确性不高。为了提高SAR船舰检测的准确率以及降低误检率,提出了一种基于高效聚合特征增强网络的SAR船舰检测方法。首先,... 详细信息

合成孔径雷达(SAR)由于散射效应以及波长和天线尺寸的分辨率限制,难以获取小尺寸目标的细节和边界信息,因此,检测准确性不高。为了提高SAR船舰检测的准确率以及降低误检率,提出了一种基于高效聚合特征增强网络的SAR船舰检测方法。首先,在主干网络中采用空间通道注意力机制,构建出高效层卷积块作为主要的特征提取模块,以增强模型的特征获取性能,提高模型对船舰目标的识别能力;其次,特征融合部分采用Inception NeXt模块来提高算法效率;最后,在主干网络以及特征提取部分之间构建出一种全局增强特征金字塔分支结构,进一步融合全局特征,避免传输过程中的低维度特征损失,以提升网络的特征融合能力,使其即使对于复杂背景下的小目标仍然能展现出可靠的检测能力。为了证明所提网络的有效性,在SSDD数据集上作了对比实验,实验结果表明,相较于YOLOv7,所提网络的准确率提升了2.5个百分点,召回率提升了9.2个百分点,交并比(IoU)阈值为0.5时的平均精度提升了6.4个百分点,IoU为0.5∶0.95时的平均精度提升了9.9个百分点。实验结果证明,所提网络在提升SAR船舰检测精度、改善误检漏检等方面有显著优势,可作为高精度的检测方法来有效应对SAR船舰检测中存在的问题。

关键词：深度学习目标检测高效聚合特征增强网络注意力机制合成孔径雷达船舰检测

基于MPSoC的轻量化汽车检测系统及硬件加速平台设计与优化

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国外电子测量技术 2024年第6期43卷 161-169页

作者：王伟王坤许圳兴付相为无锡学院江苏省集成电路可靠性技术与检测系统工程研究中心无锡214105 南京信息工程大学电子与信息工程学院南京210044

针对车辆分类检测在精度和实时性方面存在的挑战,提出了一项改进方案,以优化YOLOv5s模型,旨在实现轻量化的汽车检测。通过在MPSoC硬件架构的现场可编程门阵列(FPGA)上设计系统,成功打造了一个具备高精度、快速检测和低能耗的解决方案。... 详细信息

针对车辆分类检测在精度和实时性方面存在的挑战,提出了一项改进方案,以优化YOLOv5s模型,旨在实现轻量化的汽车检测。通过在MPSoC硬件架构的现场可编程门阵列(FPGA)上设计系统,成功打造了一个具备高精度、快速检测和低能耗的解决方案。为了使得模型更适合嵌入式设备部署,采用了MobileNetv3 Small替代YOLOv5s的主干网络,并引入卷积块注意力模块(CBAM)注意力机制和Inner-IoU Loss优化方法,使模型在轻量化的同时提升了检测精度和速度。改进后的模型相较于原始Yolov5s模型,平均精度均值(mAP)提升了14.8%,参数量减少了49.7%,模型体积减小了40.7%,计算量减少了48.9%,在NVIDIA 3060上,改进后的检测速度提升了48.8%,达到了82 fps。此外,还利用FPGA对YOLOv5s进行了硬件加速。经过优化的系统达到了45 fps的检测帧率,并保持了较高的精度和速度,这一系统易于部署,适用于智能交通系统,满足其高效实时监测的需求。

关键词：车辆分类 YOLOv5s轻量化 MobileNetv3 Small FPGA 硬件部署

Enriched endoplasmic reticulum-mitochondria interactions result in mitochondrial dysfunction and apoptosis in oocytes from obese mice

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Journal of Animal Science and Biotechnology 2018年第1期9卷 91-98页

作者： Lihong Zhao Tengfei Lu Lei Gao xiangwei fu Shien Zhu Yunpeng Hou State Key Laboratory of Agrobiotechnolocjy College of Biolocjical Sciences China Agricultural University Yuanmincjyuan west Rd 2 Bejing100193 China Key Laboratory of Animal Genetics Breeding and Reproduction College of Animal Science and Technology China Agricultural University Beijing China

Background： Maternal obesity alters oocytes and subsequent fetal metabolism. An increasing number of studies have shown that the endoplasmic reticulums（ER） or mitochondria have important effects on oocyte quality, but there has been no study of the effect of mitochondria-associated ER membranes（MAMs） on oocyte quality. The present study was designed to assess whether the level of MAM and MAM-related proteins were different in oocytes from obese and control ***： First, oocytes from mice with high-fat-diet（HFD）-induced obesity had higher levels（either greater numbers or a higher proportion for the same numbers） of MAM than oocytes from control mice. The abundance of MAM-related proteins in oocytes from obese mice was significantly greater at both the messenger RNA and protein levels, including inositol 1,4,5-trisphosphate receptor, type 1（IP3R1）, inositol 1,4,5-trisphosphate receptor,type 2（IP3 R2） and phosphofurin acidic cluster sorting protein 2（PACS-2）. further, there was an increase in mitochondrial Ca^（2＋）（[Ca^（2＋）]_m） which was associated with increased apoptosis and compromised cytoplasmic maturation in oocytes from obese mice. Down-regulation of MAM-related protein IP3R1 in oocytes from obese mice decreased [Ca^2＋]_mand apoptosis and improved cytoplasmic maturation but did not reduce the overal MAM level. However, down-regulating MAM-related protein PACS-2 in oocytes from obese mice did reduce the level of MAM and [Ca^（2＋）]_m, which decreased the rate of apoptosis and improved cytoplasmic maturation of oocytes from obese ***： It is possible that enriched MAM could increase [Ca^（2＋）]_m, and this increase has been found to be associated with increased apoptosis and compromised cytoplasmic maturation in oocytes from obese mice. This finding suggests a novel therapeutic target for obesity-induced oocyte defects.

关键词： Apoptosis IP3R1 MAM Mitochondrial Ca2+ Obesity Oocyte PACS-2

基于DFECANet的遥感图像飞机目标检测方法

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电子测量与仪器学报 2024年第2期38卷 19-29页

作者：单慧琳吕宗奎付相为胡宇翔段修贤张银胜无锡学院电子信息工程学院无锡214105 南京信息工程大学电子与信息工程学院南京210044

针对现有的遥感图像目标检测方法中对小尺寸飞机目标的检测精度不高、特征信息传递不准确、信息交互不充分等问题,提出了一种基于可辨别特征提取和上下文感知的遥感图像飞机目标检测方法。设计了以可辨别特征提取模块为主体的主干网络,... 详细信息

针对现有的遥感图像目标检测方法中对小尺寸飞机目标的检测精度不高、特征信息传递不准确、信息交互不充分等问题,提出了一种基于可辨别特征提取和上下文感知的遥感图像飞机目标检测方法。设计了以可辨别特征提取模块为主体的主干网络,用以加强对多尺度飞机目标的特征提取;引入自适应特征增强模块,选择性关注小目标、优化特征信息的传递与信息交互;并设计了特征融合上采样模块对特征图进行上采样操作,用以提升高层语义信息的准确性。在DOTAv1数据集上的检测精度达到了95.2%,相较于YOLOv5s、SCRDet、ASSD等主流算法,飞机目标的检测精度提高了3.7%~18%。此外,该方法的检测速度以及模型参数量分别为147 fps和13.4 M,相较于当前主流算法具备较强的竞争力,满足在遥感背景下对飞机目标的实时检测需求。

关键词：图像处理目标检测多尺度特征融合遥感图像特征上采样

An efficient method for the sanitary vitrification of bovine oocytes in straws

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Journal of Animal Science and Biotechnology 2014年第4期5卷 399-405页

作者： Yanhua Zhou xiangwei fu Guangbin Zhou Baoyu Jia Yi Fang Yunpeng Hou Shien Zhu National Engineering Laboratory for Animal Breeding Key Laboratory of Animal geneticsBreeding and ReproductionMinistry of AgricultureCollege of Animal Science and TechnologyChina Agricultural University Institute of Animal Genetics and Breeding College of Animal Science and TechnologySichuan Agricultural University(Chengdu Campus) State Key Laboratory for Agrobiotechnology College of Biological SciencesChina Agricultural University

Background：At present,vitrification has been widely applied to humans,mice and farm *** improve the efficiency of vitrification in straw,bovine oocytes were used to test a new two-step vitrification method in this ***：When in vitro matured oocytes were exposed to 20%ethylene glycol（EG20） for 5 min and 40%ethylene glycol（EG40） for 30 s,followed by treatment with 30%glycerol（Gly30）,Gly40 or Gly50,a volume expansion was observed in Gly30 and Gly40 but not *** indicates that the intracellular osmotic pressure after a 30 s differs between EG40 and ranged between Gly40（approximately 5.6 mol/L） and Gly50（approximately 7.0 mol/L）.Since oocytes are in EG40 just for only a short period of time（30 s） and at a lower temperature（4℃）,we hypothesize that the main function of this step in to induce *** on these results,we omitted the EG40 step,before oocytes were pretreated in EG20 for 5 min,exposed to pre-cooled（4℃） Gly50,for 30 s,and then dipped into liquid *** warming,81.1%of the oocytes survived,and the surviving oocytes developed into cleavage stage embryos（63.5%） or blastocysts（20.0%） after parthenogenetic ***：These results demonstrate that in a two-step vitrification procedure,the permeability effect in the second step is not *** is possible that the second step is only required to provide adequate osmotic pressure to condense the intracellular concentration of CPAs to a level required for successful vitrification.

关键词： Bovine Cryopreservation Oocytes Straw Vitrification

Cryopreservation of farm animal gametes and embryos:recent updates and progress

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Frontiers of Agricultural Science and Engineering 2019年第1期6卷 42-53页

作者： Zhengyuan HUANG Lei GAO Yunpeng HOU Shien ZHU xiangwei fu National Engineering Laboratory for Animal Breeding/Key Laboratory of Animal Genetics Breeding and Reproduction of Ministry of Agriculture/College of Animal Science and TechnologyChina Agricultural University State Key Laboratory of Agrobiotechnology/College of Biological Sciences China Agricultural University

Cryopreservation has undergone tremendous advances and is widely used in animal production based on decades of study of cellular permeability, freezability and empirical generalization. Several improvement are particularly important: the cryopreservation protocol has been continuously re?ned over the years to achieve greater reproductive performance; cryoprotective agents are more effective and less toxic than previously; there has been signi?cant innovation in advanced cryopreservation systems and carriers. Despite this, there are still problems that urgently require practical solutions, such as remedies for cryodamage and encouraging the use of frozen–thawed porcine sperm in pig production.

关键词： vitrification gametes embryo animal production cryoprotective agent freezability

纳米颗粒在配子及胚胎生物技术中的应用研究进展

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中国畜牧杂志 2024年第2期60卷 64-70页

作者：海桂萍李俊刘倩刘昱成万鹏程阿布力孜·吾斯曼傅祥伟新疆农业大学动物科学学院新疆乌鲁木齐830052 河北医科大学第一医院生殖中心河北石家庄050031 中国农业大学动物科学技术学院北京100193 新疆农垦科学院省部共建绵羊遗传改良与健康养殖国家重点实验室新疆石河子832000

辅助生殖技术(ART)在不孕不育诊治、濒危物种保存、良种家畜快速高效扩繁中具有重要应用价值。ART已常规开展并在世界范围内应用,但该技术面临着效率低下等挑战。非生理状态下的培养和保存可致配子和胚胎处于氧化应激状态,越来越多的研... 详细信息

辅助生殖技术(ART)在不孕不育诊治、濒危物种保存、良种家畜快速高效扩繁中具有重要应用价值。ART已常规开展并在世界范围内应用,但该技术面临着效率低下等挑战。非生理状态下的培养和保存可致配子和胚胎处于氧化应激状态,越来越多的研究表明氧化应激是影响精子获能、损伤卵母细胞及胚胎发育能力的主要因素。近年来,创新性使用纳米颗粒(NPs)的相关技术和方案有效降低了配子氧化应激水平,提高了胚胎生物技术的效率。本文系统分析了NPs进入细胞的方式、分布及影响因素,深入探讨了NPs的作用机理,归纳NPs在配子、体外胚胎生产、低温保存中的应用,以期为ART总体效率的提高提供新方法和理论依据。

关键词：纳米颗粒配子胚胎体外胚胎生产低温保存氧化应激

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Effects of the TLR4 transgene on reproductive traits and DNA methylation pattern of oocytes in ewes

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Frontiers of Agricultural Science and Engineering 2014年第4期1卷 314-320页

作者： Yi FANG xiangwei fu Junjie LI Ming DU Baoyu JIA Jinlong ZHANG Xiaosheng ZHANG Shien ZHU National Engineering Laboratory for Animal Breeding and Key Laboratory of Animal Genetics Breeding and Reproduction of Ministry of AgricultureCollege of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China College of Animal Science and Technology Agricultural University of HebeiBaoding 071000China Animal Husbandry and Veterinary Research Institute of Tianjin Tianjin 300412China

This study was conducted to systematically assess the reproductive performance of transgenic TLR4 *** the TLR4 transgenic founders(F_(0))and their positive offspring(F_(1)),hematological and reproductive parameters and the global DNA methylation level in oocytes at various stages were *** values of the physiological and biochemical parameters determined from the blood samples did not differ significantly between the transgenic and wild-type ***,the transgenic ewes showed reproductive traits similar to the wildtype *** traits included characteristics of puberty,the estrus cycle,estrus duration,gestation,the pregnancy rate and the superovulation ***,no significant differences were found between transgenic and wild-type ewes in the DNA methylation level of the oocytes at various *** summary,the preliminary evidence presented in this paper demonstrates that the presence of the TLR4 transgene did not affect the reproductive performance in sheep.

关键词： TLR4 transgenic ewe safety assessment reproductive trait oocyte DNA methylation

DNA Methylation Pattern in Pronuclear-Stage Mouse Embryos:Effect of Oocyte Vitrification

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Journal of Animal Science and Biotechnology 2011年第4期2卷 192-198页

作者： Ying Liang xiangwei fu Junjie Li Dianshuai Yuan Shien Zhu Key Laboratory of Animal Genetics Breeding and Reproduction Ministry of Agriculture and National Engineering Laboratory for Animal Breeding College of Animal Science and Technology China Agricultural University Beijing 100193 China

This study was conducted to investi- gate the pattern of DNA methylation in pronuclearstage mouse embryos derived from vitrified-warmed oocytes. Mouse oocytes at metaphase II （MII） stage of meiosis were allocated randomly into three groups： 1） untreated （control）; 2 ） exposed to vitrification solution without being plunged into liquid nitrogen （toxicity）; and 3 ） vitrified by open-pulled straw （OPS） method （vitrification）. Oocytes were fertilized in vitro （IVF）. The level of DNA methylation was examined at 8 hpf （hours post-fertilization） by immunofluorescence using an anti-5-methylcytosine （5-MeC） monoclonal antibody and fluorescein isothiocyanate （FITC）-conjugated goat anti-mouse IgG. After IVF, rates of 2-cell embryos （ 51.39% ） and blastocysts （35.82%） for vitrified-warmed oocytes were lower （P〈0.01） than that for control （70.83%, 47.82% ） or vitrification solution treated （64.80%, 46.29% ） oocytes. At 8 hpf, there were more （P 〈 0.05） pronuclear-stage oocytes in which syngamy of pronuclei had not occurred in the vitrifica- tion group. In addition, 5-MeC fluorescent intensities for the female pronucleus and zygote were lower in the vitrification group （P 〈0.01 ） compared to pronuclear-stage embryos in the control and toxicity groups. In conclusion, oocyte vitrification causes a reduction in global genomic methylation in the female pronucleus and zygote, resulting in delayed fusion of pronuclei and compromising the developmental potential of mouse zygotes and embryos.

关键词： DNA methylation mouse oocyte open-pulled straw vitrification pronucleus