An efficient method for the sanitary vitrification of bovine oocytes in straws

作     者：Yanhua Zhou Xiangwei Fu Guangbin Zhou Baoyu Jia Yi Fang Yunpeng Hou Shien Zhu 

作者机构：National Engineering Laboratory for Animal BreedingKey Laboratory of Animal geneticsBreeding and ReproductionMinistry of AgricultureCollege of Animal Science and TechnologyChina Agricultural University Institute of Animal Genetics and BreedingCollege of Animal Science and TechnologySichuan Agricultural University(Chengdu Campus) State Key Laboratory for AgrobiotechnologyCollege of Biological SciencesChina Agricultural University 

出 版 物：《Journal of Animal Science and Biotechnology》 (畜牧与生物技术杂志（英文版）)

年 卷 期：2014年第5卷第4期

页      面：399-405页

核心收录：

学科分类：0905[农学-畜牧学] 09[农学] 

基　　金：supported by the National "863" Project Foundation of China(No.2011AA100303) the National Science and Technology Support Projects of China(No.2011BAD19B01) 

主　　题：Bovine Cryopreservation Oocytes Straw Vitrification 

摘      要：Background：At present,vitrification has been widely applied to humans,mice and farm *** improve the efficiency of vitrification in straw,bovine oocytes were used to test a new two-step vitrification method in this ***：When in vitro matured oocytes were exposed to 20%ethylene glycol（EG20） for 5 min and 40%ethylene glycol（EG40） for 30 s,followed by treatment with 30%glycerol（Gly30）,Gly40 or Gly50,a volume expansion was observed in Gly30 and Gly40 but not *** indicates that the intracellular osmotic pressure after a 30 s differs between EG40 and ranged between Gly40（approximately 5.6 mol/L） and Gly50（approximately 7.0 mol/L）.Since oocytes are in EG40 just for only a short period of time（30 s） and at a lower temperature（4℃）,we hypothesize that the main function of this step in to induce *** on these results,we omitted the EG40 step,before oocytes were pretreated in EG20 for 5 min,exposed to pre-cooled（4℃） Gly50,for 30 s,and then dipped into liquid *** warming,81.1%of the oocytes survived,and the surviving oocytes developed into cleavage stage embryos（63.5%） or blastocysts（20.0%） after parthenogenetic ***：These results demonstrate that in a two-step vitrification procedure,the permeability effect in the second step is not *** is possible that the second step is only required to provide adequate osmotic pressure to condense the intracellular concentration of CPAs to a level required for successful vitrification.