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Recognition mechanism and sequence optimization of organophosphorus pesticides aptamers for better monitoring contaminations in food

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Food Science and Human Wellness 2023年第5期12卷 1708-1715页

作者： Pengfei Chen Chaoqiong Hu Xuan Tao Zheng Zhou Lijun Wang Xiao Yang Zhenming Che xianggui Chen Yukun Huang Chongqing Key Laboratory of Speciality Food Co-Built by Sichuan and Chongqing School of Food and Biological EngineeringXihua UniversityChengdu 610039China Chengdu Brilliant Pharmaceutical Co. Ltd.Chengdu 610041China Key Laboratory of Food Non-Thermal Processing Engineering Technology Research Center of Food Non-Thermal ProcessingYibinXihua University Research InstituteYibin 644004China

Aptamers as a kind of biological recognition element have shown great potential in monitoring and the rapid quantification of organophosphorus pesticides(OPPs). However, molecules of OPPs are structurally similar and original aptamers selected by systematic evolution of ligands by exponential enrichment are usually long-chain bases, which hamper the further application under OPPs-aptamer recognition. The aim of the research was to develop a new strategy to design oligonucleotide sequences for binding OPPs by combination of experimental and molecular modeling methods. 3D models of aptamers binding OPPs were constructed, and binding energy and the most probable binding site for the OPPs were then determined by molecular docking, and the binding sites were further confirmed by the results of 2-AP replaced experiments. Based on the docking results, a new aptamer for detection 4 representative OPPs with only 29 bases was designed by reasonable truncation and mutation of the reported aptamer(named S4-29). The interaction between this new aptamer and OPPs were analyzed by molecular docking, microscale thermophoresis, circular dichroism and fluorometric analysis. The results revealed that the new aptamer exhibit more superior recognition performance to OPPs, which can be promote the monitoring ability of OPPs contaminations in food.

关键词： Organophosphorus pesticides Aptamer Recognition mechanism Sequence optimization

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某核电厂蒸汽供能改造对汽轮机组运行的影响分析与优化研究

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电工技术 2024年第5期 217-220页

作者：黄兵张祥贵张现傲陈彬陈卫华江苏核电有限公司江苏连云港222000

通过模拟机验证分析机组蒸汽供能改造后系统运行参数的变化,评估改造对机组运行的影响并提出运行优化建议,供设计参考以完善蒸汽供能改造后的机组控制。从机组运行控制方式、功率失配量计算、蒸汽凝结水回水、汽轮发电机快速升负荷、发... 详细信息

通过模拟机验证分析机组蒸汽供能改造后系统运行参数的变化,评估改造对机组运行的影响并提出运行优化建议,供设计参考以完善蒸汽供能改造后的机组控制。从机组运行控制方式、功率失配量计算、蒸汽凝结水回水、汽轮发电机快速升负荷、发电机功率下降方面对机组控制逻辑进行分析,评估上述因素对机组运行的影响,对核电厂蒸汽供能改造后的运行控制提出优化建议,为蒸汽供能改造的核电机组或新建核能供热(供汽)机组提供设计参考。

关键词：蒸汽供能运行控制控制逻辑

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准东煤田西山窑组煤层地震反射特征及其地质意义

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华北地震科学 2024年第1期42卷 37-44,50页

作者：王秀东孔祥贵鲁楠陈亦磊王琛山东省煤田地质规划勘察研究院济南251000 山东泰山资源勘查有限公司济南251000

应用陆相聚煤盆地沉积学的理论,通过对地震反射波特征的认识,揭示了地震响应所反映的煤岩层赋存形态的地质意义;结合钻井岩心描述、测井资料等构建初始模型,反演煤层反射波物性参数,建立地震资料与复煤层宏观结构的联系,描述了煤层的赋... 详细信息

应用陆相聚煤盆地沉积学的理论,通过对地震反射波特征的认识,揭示了地震响应所反映的煤岩层赋存形态的地质意义;结合钻井岩心描述、测井资料等构建初始模型,反演煤层反射波物性参数,建立地震资料与复煤层宏观结构的联系,描述了煤层的赋存形态。

关键词：陆相盆地地震反射特征地质意义煤层宏观结构新疆准噶尔盆地

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宫内高血糖对新生儿炎症因子水平及婴儿过敏性疾病发生的影响

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新医学 2024年第6期55卷 437-442页

作者：陈晓玲黄向贵周泳宜罗立荣黄为民南方医科大学第一临床医学院(南方医院)新生儿科广东广州510515 广州市红十字会医院儿科广东广州510220 广州市红十字会医院产科广东广州510220

目的探讨宫内高血糖暴露对新生儿脐带血炎症细胞因子水平及婴儿过敏性疾病发生的影响。方法选择112例产妇及其新生儿,根据孕期血糖情况分为宫内高血糖暴露组(55例)和无宫内高血糖暴露组(57例),比较2组新生儿出生时脐带血中的炎症细胞... 详细信息

目的探讨宫内高血糖暴露对新生儿脐带血炎症细胞因子水平及婴儿过敏性疾病发生的影响。方法选择112例产妇及其新生儿,根据孕期血糖情况分为宫内高血糖暴露组(55例)和无宫内高血糖暴露组(57例),比较2组新生儿出生时脐带血中的炎症细胞因子水平差异。随访1年,比较2组婴儿过敏性疾病如特应性皮炎、婴幼儿喘息等的发生率,分析宫内高血糖对新生儿免疫力及婴儿过敏性疾病的影响。结果 2组新生儿脐带血IL-1β、IL-2、IL-6、IL-8、IL-10、IL-12、TNF-α质量浓度比较差异均无统计学意义(P均> 0.05)。宫内高血糖暴露组新生儿脐带血IL-4质量浓度为(30.180±8.648)pg/mL,高于无宫内高血糖暴露组的(22.500±10.100)pg/mL(P 0.05)。结论宫内高血糖暴露对新生儿脐带血细胞因子IL-4质量浓度有一定影响,这或许能为今后过敏性疾病早期干预提供依据。

关键词：新生儿脐带血细胞因子宫内高血糖过敏

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Analysis of the genomic landscape of primary central nervous system lymphoma using whole-genome sequencing in Chinese patients

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Frontiers of Medicine 2023年第5期17卷 889-906页

作者： xianggui Yuan Teng Yu Jianzhi Zhao Huawei Jiang Yuanyuan Hao Wen Lei Yun Liang Baizhou Li Wenbin Qian Department of Hematology the Second Affiliated HospitalZhejiang University School of MedicineHangzhou310009China Department of Pathology the Second Affiliated HospitalZhejiang University School of MedicineHangzhou310009China National Clinical Research Center for Hematologic Diseases the First Affiliated Hospital of Soochow UniversitySuzhou215006China Cancer Institute(Key Laboratory of Cancer Prevention and Intervention China National Ministry of Education)the Second Affiliated Hospital of Zhejiang University School of MedicineHangzhou310009China

Primary central nervous system lymphoma(PCNSL)is an uncommon non-Hodgkin’s lymphoma with poor *** study aimed to depict the genetic landscape of Chinese ***-genome sequencing was performed on 68 newly diagnosed Chinese PCNSL samples,whose genomic characteristics and clinicopathologic features were also *** variations were identified in all patients with a mean of 349,which did not significantly influence *** loss occurred in all samples,while gains were detected in 77.9%of the *** high level of copy number variations was significantly associated with poor progression-free survival(PFS)and overall survival(OS).A total of 263 genes mutated in coding regions were identified,including 6 newly discovered genes(ROBO2,KMT2C,CXCR4,MYOM2,BCLAF1,and NRXN3)detected in≥10%of the ***79B mutation was significantly associated with lower PFS,TMSB4X mutation and high expression of TMSB4X protein was associated with lower OS.A prognostic risk scoring system was also established for PCNSL,which included Karnofsky performance status and six mutated genes(BRD4,EBF1,BTG1,CCND3,STAG2,and TMSB4X).Collectively,this study comprehensively reveals the genomic landscape of newly diagnosed Chinese PCNSLs,thereby enriching the present understanding of the genetic mechanisms of PCNSL.

关键词： primary central nervous system lymphoma whole-genome sequencing TMSB4X copy number variation gene utation

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4种不同来源花青素的稳定性及抗氧化性比较

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食品研究与开发 2024年第4期45卷 31-39,67页

作者：马孟佳赵娟陈鹏飞陈祥贵黄玉坤西华大学食品与生物工程学院食品微生物四川省重点实验室四川成都610039 四川合泰新光生物科技有限公司四川成都610000 川渝共建特色食品重庆市重点实验室四川成都610039

为更好地探索不同原料的花青素作为食品着色剂和营养剂的潜力,对蓝莓、紫薯、紫甘蓝、黑豆的花青素进行提取,对4种原料花青素含量进行比较,并探究4种原料花青素的抗氧化性以及在不同因素下(光照、pH值、温度、金属离子、氧化剂、还原剂... 详细信息

为更好地探索不同原料的花青素作为食品着色剂和营养剂的潜力,对蓝莓、紫薯、紫甘蓝、黑豆的花青素进行提取,对4种原料花青素含量进行比较,并探究4种原料花青素的抗氧化性以及在不同因素下(光照、pH值、温度、金属离子、氧化剂、还原剂、葡萄糖浓度、蛋白质)的稳定性。结果表明,花青素含量排序为紫甘蓝(435.84 mg/L)>紫薯(401.77 mg/L)>蓝莓(388.08 mg/L)>黑豆(177.34 mg/L)。稳定性最佳的原料花青素为紫甘蓝花青素,抗氧化性最强的为蓝莓花青素(羟自由基清除率、DPPH自由基清除率、ABTS^(+)自由基清除率分别为76.41%、83.05%、88.13%,总还原能力测得吸光值为2.04)。

关键词：花青素色素保存率稳定性自由基清除率抗氧化性

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Composite B-cell and T-cell lymphomas:clinical,pathological,and molecular features of three cases and literature review

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2023年第8期24卷 711-722页

作者： Xueli JIN Hui LIU Jing LI Xibin XIAO xianggui YUAN Panpan CHEN Boxiao CHEN Yun LIANG Fengbo HUANG Department of Hematology the Second Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhou 310009China Department of Pathology the Second Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhou 310009China Department of Nuclear Medicine the Second Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhou 310009China School of Medicine Zhejiang UniversityHangzhou 310029China

Composite lymphoma(CL)involving B-cell lymphoma and T-cell lymphoma is extremely ***,we report three such cases using immunohistochemistry,flow cytometry,and the next-generation sequencing(NGS)to identify the pathological and molecular characteristics of *** the first case,the patient was admitted to hospital for generalized pruritic maculopapular rash over the whole *** excisional biopsy of the skin lesions showed T-cell *** the same time,the staging bone marrow(BM)biopsy revealed a diffuse large B-cell lymphoma(DLBCL).After R-CHOP(rituximab,cyclophosphamide,doxorubicin,vincristine,and prednisone)therapies,the patient produced a good response with substantial dissipation of the rashes and relief of *** other two patients were admitted to hospital due to lymphadenopathy and were diagnosed with DLBCL and follicular lymphoma(FL)after core needle biopsy of lymph nodes,BM biopsy,BM aspiration,and flow *** R-CHOP and R-COP(rituximab,cyclophosphamide,vincristine,and prednisone)therapies,they achieved complete remission unconfirmed(CRu)and complete remission(CR).However,one or two years later,they suffered a relapse of *** shocking fact was that re-biopsy of lymphadenopathy revealed peripheral T-cell lymphoma(PTCL)and angioimmunoblastic T-cell lymphoma(AITL).NGS findings identified DNA methyltransferase 3a(DNMT3a),isocitrate dehydrogenase 2(IDH2),Ras homolog gene family,member A(RHOA),splicing factor 3B subunit 1(SF3B1),and tumor protein p53(TP53)*** immunochemotherapy,these patients achieved CRu and CR ***,they suffered a second relapse of T-cell ***,they died due to progression of *** found that the occurrence of CL is associated with Epstein-Barr virus infection and DNMT3a,IDH2,and TP53 mutations,and the prognosis of the disease is closely related to the T-cell lymphoma components.

关键词： Composite lymphoma B-cell lymphoma T-cell lymphoma

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An Electrocatalytic Strategy for Dehydrogenative[4+2]Cycloaddition over a Cobalt-Based Catalyst

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Chinese Journal of Chemistry 2022年第23期40卷 2741-2746页

作者： Xiaomeng Xue Ye Wang Lina Zhou Ruixiang Ge Jiangrong Yang xianggui Kong Ming Xu Zhenhua Li Lina Ma Haohong Duan State Key Laboratory of Chemical Resource Engineering Beijing University of Chemical TechnologyBeijing 100029China Department of Chemistry Tsinghua UniversityBeijing 100084China State Key Laboratory of Catalytic Materials and Reaction Engineering Research Institute of Petroleum ProcessingSiNOPECBeijing 100083China

Exploring the anodic reaction to substitute conventional oxygen evolution reaction(OER)for the synthesis of complex pharmaceutical molecules is highly ***,we report an electrocatalytic strategy for dehydrogenative[4+2]cycloaddition of N,N-dialkylanilines with maleimides via dual functionalization of both C(sp3)-H and C(sp2)-H bonds,by using an electrochemically activated cobalt carbonate hydroxide hydrate supported on carbon cloth(CCHH-A/CC),affording various tetrahydroquinolines with high *** electrochemical transformation proceeds with high activity and stability,as well as good substrate *** study shows thatα-aminoalkyl radical exists in the electrooxidation *** strategy shows significant potential for the synthesis of valuable chemicals by using an electrocatalytic strategy.

关键词： Cycloaddition C-H activation Dehydrogenation Eletrooxidation reactions Cobalt-based catalyst

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Molecular cloning and characterization of the endothelin 3 gene in black bone sheep

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Journal of Animal Science and Biotechnology 2018年第4期9卷 847-857页

作者： Hesham Y.A.Darwish Yuanyuan Zhang Kai Cui Zu Yang Deping Han xianggui Dong Huaming Mao Weidong Deng Xuemei Deng National Engineering Laboratory for Animal Breeding and Key Laboratory of Animal Genetics Breeding and Reproduction of the Ministry of AgricultureChina Agricultural University Animal Production Research Institute Agricultural Research Center Ministry of Agriculture and Land Reclamation College of Veterinary Medicine China Agricultural University College of Animal Science and Technology Yunnan Agricultural University

Background: Black bone sheep was first discovered in Yunnan province of China in 1970, with unique black pigmentation on the body and internal organs. Endothelin 3(EDN3) has been known as a key gene causing hyperpigmentation in black bone chicken, the Silky ***: In this study, EDN3 was employed as a candidate gene for regulating black color pigmentation. First,EDN3 was cloned from sheep to obtain the full-length cDNA by using the rapid amplification of cDNA ends(RACE).Genomic EDN3 was screened and a total of thirty predicted single nucleotide polymorphisms(SNPs) were genotyped for allele and genotype frequency analysis in a case-control study involving two black bone sheep populations. Genomic copy number analysis of EDN3 in sheep was conducted to measure the variation in copy number. EDN3 expression levels were observed among the groups in adult liver, lymph node, and kidney tissues, as well as embryo kidney samples. Also, among the tissues of black bone and non-black bone ***: The size of the full-length cDNA was 1,578 bp, which included 426 bp of 5′-untranslated region(5′-UTR),an open reading frame(ORF) of 639 bp encoding a protein of 212 amino acids, and a 3′-UTR of 513 bp. Genotype and allele frequencies of all the discovered SNPs were found insignificantly different in black bone and non-black bone sheep(P > 0.05). Genomic copy number analysis of EDN3 in sheep revealed no significant difference between the two sheep groups. No significant variations were found in the adult liver and kidney embryo samples. However,the expression in lymph node and kidney tissue was significantly higher in black bone sheep than that in non-black bone sheep(P < 0.05). Significant variations in the EDN3 expression levels were observed among the tissues of nonblack bone ***: The findings of the present study indicate that unlike in Silky chickens, EDN3 is not responsible for hyperpigmentation but may play a key functional role in immune and excretory

关键词： Black pigmentation Expression level Sequence analysis SNP

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Construction of self-sensitized LiErF4:0.5%Tm^3+@LiYF4 upconversion nanoprobe for trace water sensing

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Nano Research 2020年第10期13卷 2803-2811页

作者： Ling Zhang Xiaodan Li Wang Wang Xu Zhao Xu Yan Chenguang Wang Haoqiang Bao Yang Lu xianggui Kong Fengmin Liu Xiaomin Liu Geyu Lu State Key Laboratory of Integrated Optoelectronics hilin Key Laboratory of Advanced Gas SensorsCollege of Electronic Science and EngineeringJjilin UniversityChangchun 130012China Department of Respiratory Medicine The First Hospitaljilin UniversityChangchun 130021China State Key Laboratory of Luminescence and Applications Changchun Institute of OpticsFine Mechanics and PlhysicsChinese Academy of ScienceChangchun 130033China

LiErF4 was commonly used as a dipolar-coupled antiferromagnet,and was rarely considered as a luminescent ***,we achieved the strong red upconversion emission of LiErF4 simply by an inert shell coating,i.e.,LiErF4@*** to the unique and intrinsic ladder-like energy levels of Er3+ions,this LiErF4 core–shell nanostructures present red emission(~650 nm)under multi-band excitation in the near-infrared(NIR)region(~808,~980,and^1,530 nm).A brighter and monochromic red emission can be further obtained via doping 0.5%Tm3+into the LiErF4 core,i.e.,LiErF4:0.5%Tm3+@*** enriched Er3+ions and strong monochromic red emission natures make LiErF4:0.5%Tm3+@LiYF4 nanocrystals very sensitive for trace water probing in organic solvents with detection limit of 30 ppm in acetonitrile,50 ppm in dimethyl sulfoxide(DMSO),and 58 ppm in N,N-dimethylformamide(DMF)under excitation of 808 *** to their superior chemical and physical stability,these nanoprobes exhibit excellent antijamming ability and recyclability,offering them suitable for real-time and long-term water monitoring.

关键词： LiErF4 water detection upconversion emission sensor self-sensitized

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