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Characteristics and Kinetics of Nitrobenzene Reduction by Sucrose-modified Nanoiron

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Chemical Research in Chinese Universities 2013年第4期29卷 765-770页

作者： LI hui zhao Yong-sheng zhao Ran MA Bai-wen CHEN Zi-fang SU Yan ZHOU Rui Key Laboratory of Groundwater Resources and Environment Ministry of Education Jilin University Changchun 130021 P. R. China Department of Chemistry & Biochemistry College of Liberal Arts & Science Arizona State University Phoenix 85001 USA State Key Laboratory of Environmental Aquatic Chemistry Research Center for Eeo-Environmental Sciences Chinese Academy of Sciences Beijing 100085 P. R. China University of Chinese Academy of Sciences Beijing 100049 P. R. China

Sucrose-modified nanoscale zero-valent iron（SM-NZVI） was prepared with liquid phase reducing method, and characterized by transmission electron microscopy（TEM） and X-ray diffraction（XRD） for the shape and structure The size of SM-NZVI particles was about 100--150 nm and they displayed better dispersity. The degradation of ni- trobenzene（NB） in water by SM-NZVI was carried out in batch experiments. The results indicate that the efficiency of NB reduction via SM-NZVI increased by 44.24% compared with that via NZV, I, and coexistent Ca2＋, Mg2＋, HCO3- and other ions showed little effect on NB reduction but negative influence on aniline（AN） production. Furthermore, the kinetic researches indicate that NB reduction with SM-NZVI could be described by pseudo first-order kinetic model at different initial pH values and iron dosage. The oxidation products of iron were mainly Fe304 and Fe203 with irregular shape.

关键词： Nanoiron Sucrose Nitrobenzene Reduction reaction Kinetic

Line profile analysis of an astronomical spectrograph with a laser frequency comb

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Research in Astronomy and Astrophysics 2014年第8期14卷 1037-1045页

作者： Fei zhao Gang zhao Gaspare Lo Curto hui-Juan Wang Yu-Juan Liu Liang Wang Wei Wang Key Laboratory of Optical Astronomy National Astronomical ObservatoriesChinese Academy of Sciences University of Chinese Academy of Sciences Shandong Provincial Key Laboratory of Optical Astronomy and Solar-Terrestrial Environment School of Space Science and PhysicsShandong University European Southern Observatory Karl-Schwarzschild-Str. 285748 GarchingGermany

We present a study of the spectral line shape associated with a High Resolution Spectrograph on the 2.16 m telescope at the Xinglong Observing Station of National Astronomical Observatories, Chinese Academy of Sciences. This measurement is based on modeling the instrumental line shape obtained by unresolved modes from a Yb-fiber mode-locked laser frequency comb. With the current repetition rate of 250 MHz and 26 GHz mode spacing on the spectrograph, we find the absolute variation of the line center, 0.0597 pixel in the direction of the CCDs, and 0.00275 pixel （～3 m s^-1） for relative variation in successive exposures on a short timescale. A novel double-Gaussian model is presented to improve the quality of the fit by a factor of 2.47 in a typical single exposure. We also use analysis with raw moments and central moments to characterize the change in line shape across the detector. A trend in charge transfer efficiency can be found on the E2V 4096 ×4096 CCD that provides a correction for wavelength calibration aiming to reach a level of precision for radial velocity below 1 m s^- 1.

关键词： techniques radial velocities -- instrumentation laser frequency comb

MP3RNA-seq:Massively parallel 3’end RNA sequencing for high-throughput gene expression profiling and genotyping

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Journal of Integrative Plant Biology 2021年第7期63卷 1227-1239页

作者： Jian Chen xiangbo Zhang Fei Yi xiang Gao Weibin Song Haiming zhao Jinsheng Lai State Key Laboratory of Plant Physiology and Biochemistry National Maize Improvement CenterDepartment of Plant Genetics and BreedingChina Agricultural UniversityBeijing 100193China Center for Crop Functional Genomics and Molecular Breeding China Agricultural UniversityBeijing 100193China

Transcriptome deep sequencing(RNA‐seq)hasbecome a routine method for global geneexpression ***,its application tolarge‐scale experiments remains limited by costand labor *** we describe amassively parallel 3′end RNA‐seq(MP3RNA‐seq)method that introduces unique samplebarcodes during reverse transcription to permitsample pooling immediately following this ***3RNA‐seq allows for handling of hun-dreds of samples in a single experiment,at acost of about$6 per sample for libraryconstruction and ***3RNA‐seq iseffective for not only high‐throughput geneexpression profiling,but also *** its utility,we applied MP3RNA‐seqto 477 double haploid lines of *** iden-tified 19,429 genes expressed in at least 50%ofthe lines and 35,836 high‐quality singlenucleotide polymorphisms for *** with these data,we performedexpression and agronomic trait quantitativetrait locus(QTL)mapping and identified 25,797expression QTLs for 15,335 genes and 21 QTLsfor plant height,ear height,and relative *** conclude that MP3RNA‐seq is highlyreproducible,accurate,and sensitive forhigh‐throughput gene expression profiling andgenotyping,and should be generally applicableto most eukaryotic species.

关键词： gene expression profiling genotyping maize MP3RNA‐seq quantitative trait loci

Preparation and tribological behavior of electrodeposited Ni-W-GO composite coatings

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Rare Metals 2019年第7期38卷 695-703页

作者： Xue-hui Zhang Xiao-Xian Li Wei-Jiang Liu Yu-Qi Fan Hao Chen Tong-xiang Liang School of Materials Science and Engineering Jiangxi University of Science and Technology Ganzhou 341000 China School of Metallurgy and Chemical Engineering Jiangxi University of Science and Technology Ganzhou 341000 China

Ni-W-GO composite coatings were successfully plated on 45# steel substrate by co-electrodeposition technique in a Ni-W electrolyte solution, with different contents of graphene oxide (GO) nanoparticles in suspen? sion. The structure, phase composition and surface morphology of as-plated composite coatings were characterized by Raman, X-ray diffraction (XRD), scanning electron microscopy (SEM) attached with energy disperse spectroscopy (EDS), respectively. The hardness and tribological behavior of the present coatings were also evaluated by Vickers Hardness tester and high-speed reciprocating friction and wear tester, and the wear mechanism was discussed as well. The results show that layer-structured GO nanoparticles significantly affect the microstructure and grain size of the Ni-W-GO composite coatings. Meanwhile, GO nanoparticles embedded in Ni- W-GO coatings can obviously improve the hardness and wear resistance in comparison with the corresp on ding Ni- W coatings. The highest microhardness and wear resistance of Ni-W-GO composite coatings are obtained with 0.15 g·L^-1 GO employing.

关键词： Ni-W-GO composite coatings Graphene oxide Electrodeposition Microstructure Tribological behavior Microhardness

Loading of tacrolimus containing lipid based drug delivery systems into mesoporous silica for extended release

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Asian Journal of Pharmaceutical Sciences 2016年第6期11卷 751-759页

作者： Li Liu Jia Li Mei-hui zhao hui Xu Lin-sen Li Shao-ning Wang School of Pharmacy Shenyang Pharmaceutical UniversityShenyang 110016China Department of Biochemistry Shenyang Medical CollegeShenyang 110034China School of Pharmaceutical Engineering Shenyang Pharmaceutical UniversityShenyang 110016China

Many studies had been focused on designing tacrolimus sustained release preparations based on solid dispersion technique, but no one had tried to employ mesoporous silica as the carrier material to realize this goal. The purpose of this study was to develop a novel, simple and environmental friendly drug loading method with mesoporous silica to obtain tacrolimus sustained-release preparation. Tacrolimus was firstly dissolved in the molten mixed lipid composed of Compritol 888 ATO and Gelucire 50/13 to prepare a drug loaded lipid-based drug delivery systems(LBDDS), then the liquid LBDDS was adsorbed by mesoporous silica to transfer the liquid into solid powder, ie. the tacrolimus sustained release silica-lipid hybrid(SLH). The SLH was characterized by SEM, CLSM, XRPD and DSC, and the in vitro drug release was tested using a paddle method. SEM and CLSM observation showed that the LBDDS was efficiently distributed throughout the pores of the silica. The results of DSC and XRPD illustrated that the lipid existed inside the silica at amorphous state. The drug-loaded SLH showed good flowability, compressibility, compactibilty and two-phase in vitro drug release process within 24 hours, which did not change obviously even after storage at 40 °C for 10 *** present study provided a novel and simple method to prepare tacrolimus sustained release powder, which provided a feasible solution to solidify the liquid LBDDS of not only extended drug release behavior, but also improved stability and micromeritic properties.

关键词： Tacrolimus Lipid-based drug delivery system Mesoporous silica Silica-lipid hybrid Sustained-release

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气相色谱法测定白酒中的乙酸、己酸含量

实验室检测

实验室检测 2024年第1期2卷 17-22页

作者：彭晓鲲黄小芳肖慧徐鸿翔赣州市综合检验检测院赣州341000

目的准确测量在白酒生产和质量控制中的两个关键成分:乙酸和己酸。方法采用直接进样-气相色谱技术(GC),快速且准确地同时测定了白酒中的乙酸和己酸含量。结果利用本方法,乙酸的检出限达到了0.012 g/L,己酸的检出限为0.0050 g/L。所有相... 详细信息

目的准确测量在白酒生产和质量控制中的两个关键成分:乙酸和己酸。方法采用直接进样-气相色谱技术(GC),快速且准确地同时测定了白酒中的乙酸和己酸含量。结果利用本方法,乙酸的检出限达到了0.012 g/L,己酸的检出限为0.0050 g/L。所有相关系数均超过0.999。相对标准偏差(RSD)分别为0.32%(乙酸)和0.33%(己酸),加标回收率分别为96.15%(乙酸)和96.69%(己酸)。结论本研究提出的新检测方法,不仅克服了传统检测方法和气相色谱在诸多方面的限制,而且展示了高效率、高灵敏度和优良选择性。这一方法对于提升白酒的生产工艺、确保产品风味的一致性和安全性具有重要的实际意义。

关键词：气相色谱法乙酸己酸白酒定量分析

Book review “Continuous Cover Forestry – Theories, Concepts, and Implementation” by Arne Pommerening

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Forest Ecosystems 2023年第6期10卷 774-775页

作者： Zhonghua zhao Gangying hui Chinese Academy of Forestry Beijing 100091China

Arne Pommerening,Continuous Cover Forestry:Theories,Concepts,and ***(2023),pp.416;ISBN:978-1-119-89532-9(E-BOOK),ISBN:978-1-119-89530-5(PRINT).Forests are the largest,most complex,and most diverse terrestrial ecosystems,known as the overall regulator of nature and the“lungs of the earth”.They play an important role in maintaining global ecological balance and biodiversity,mitigating climate change,and promoting economic ***,strengthening the protection and cultivation of forest resources is an important way to achieve sustainable *** around the world are actively exploring and developing ways to achieve sustainable forest management.

关键词： PRINT forest strengthening

Comprehensive silk gland multi-omics comparison illuminates two alternative mechanisms in silkworm heterosis

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Zoological Research 2022年第4期43卷 585-596页

作者： Han Xu Lei Chen Xiao-Ling Tong Hai Hu Li-Yuan Liu Gui-Chun Liu Ya-Nan Zhu Ruo-Ping zhao Wen Wang Fang-Yin Dai Xin Li hui xiang School of Medicine Shenzhen Campus of Sun Yat-Sen UniversityShenzhenGuangdong 518107China Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology Guangzhou Key Laboratory of Insect Development Regulation and Application ResearchInstitute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhouGuangdong 510631China School of Ecology and Environment Northwestern Polytechnical UniversityXi’anShaanxi 710072China State Key Laboratory of Silkworm Genome Biology Key Laboratory of Sericultural Biology and Genetic BreedingMinistry of Agriculture and Rural AffairsCollege of BiotechnologySouthwest UniversityChongqing 400715China State Key Laboratory of Genetic Resources and Evolution Kunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan 650223China

Heterosis is a common phenomenon in plants and animals with diverse underlying ***,we applied two widely used silkworm hybrid systems and performed multi-omics analysis to identify possible intrinsic associations between different hybrid strategies and epigenetic mechanisms with silkworm *** found significant differences in the silk gland transcriptomic landscape between the two systems,including differentially expressed genes and expression patterns in the hybrid offspring compared to their *** the quaternary hybrid system,hybrid vigor was primarily due to upregulated genes and the parent-dominant upregulated expression pattern,involving multiple transport processes,cellular nitrogen compound catabolism,glucose metabolism,and tricarboxylic acid *** the binary system,hybrid vigor was mainly due to the down-regulated genes and transgressively down-regulated expression pattern,mainly involving basic nitrogen synthesis metabolism and body *** also demonstrated that DNA methylation may affect hybrid vigor by regulating the expression of several heterosis-related ***,this study revealed two alternative mechanisms that may contribute to silkworm heterosis,both of which facilitate the efficient utilization of energy and nitrogen for silk production.

关键词： Heterosis Silkworm Hybrid systems Multi-omics Silk production

Purification and Characterization of a New Heme-Binding （HBP59） from the Mutant Strain DJ35 of Azotobacter vinelandii

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Journal of Integrative Plant Biology 2007年第3期49卷 336-342页

作者： Shao-Min Bian Huang-Ping Wang hui-Na Zhou Ying zhao Jian-Feng zhao Ju-Fu Huang Key Laboratory of Photosynthesis and Environmental Molecular Physiology Institute of Botany the Chinese Academy of Sciences Beijing 100093 China Bioengineering College Fujian Normal University Fuzhou 350007 China Graduate School the Chinese Academy of Sciences Beijing 100039 China

A new protein, an approximately 59-kDa monomer containing iron atoms, was first isolated from the mutant strain DJ35 of Azotobacter vinelandii Llpmann. After analysis by matrix-assisted laser desorptlon ionization time-of-flight mass spectrometry, the protein was Identified as the product of a predicted gene. Thus, the protein was tentatively called HBP59. Its absorption spectra （ABS） In the reduced state exhibited three peaks at 421,517, and 556 nm and the maximal peak was shifted from 421 to 413 nm after exposure of HBP59 to air. The Soret circular dichrolsm （CD） spectrum of HBP59 In the reduced state displayed four positive peaks at 364, 382, 406, and 418 nm and two negative peaks at 398 and 433 nm; the △ε （CD extinction coefficient） values of these peaks were found to be 0.92, 0.58, 0.87, 0.72, -0.65 and -1.12 L/mol per cm, respectively. Titration with heme showed that the protein has 0.1 heme molecules/protein molecule. After HBP59 had fully Interacted with heme, Its maximal ABS value and Soret CD Intensity were increased by approximately 10-fold compared with values before Interaction. Therefore, It seems that one molecule of HBP59 can be interacted with only one heme. These results indicate that HBP59 contains heme with low spin and may be Involved In heme utilization or adhesion.

关键词： absorption spectra Azotobacter vinelandii characterization by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) circular dichroism spectra and titration heme-binding protein (HBP59) mutant strain DJ35 purification